• Title/Summary/Keyword: slime

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The Cell and Genetic Characteristics of Slime Forming Bacteria on Antibiotic Resistance in the Paper-making Process

  • Kim, Eun-Hee;Kang, Kyu-Young;Jo, Byoung-Muk;Oh, Jung-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.3
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    • pp.26-33
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    • 2002
  • The seven strains, Pseudomonas paucimobilis, Pseudomonas cepacia, Staphylococcus auricularis, Staphylococcus saprophyticus, Acidovorax spp., Acinetobacter calcoaceticus, and Actinobacillus capsulatus were tested with three slimicides. Most of the tested bacteria were inhibited with slimicide K (an isothiazolin based compound), even at its low concentration, except for Actinobacillus capsulatus and Staphylococcus auricularis. Both slimicides B (an organic bromine based compound) and S (aldehydes) also couldn't prevent these two strains even at their highest concentration. Five different sizes of plasmid DNA were isolated from Actinobacillus capsulatus. Staphylococcus auricularis, a gram-positive bacteria, showed the slimy substances around its cell distinctively. The results suggest that two strains, Actinobacillus capsulatus, Staphylococcus auricularis, have presumably developed a resistance to the slimicide, by plasmid DNA or slimy substance. Our findings also suggest that not only gram-negative bacteria, but also gram-positive bacteria should not be neglected

Isolation and Characterization of Oxygen-tolerant Mutant of Bifidobacterium longum. (Bifidobacterium longum 산소변이주의 분리와 변이주의 산소내성)

  • 안준배;김광엽;박종현
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.476-482
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    • 1998
  • Growth sensitivity of bifidobacteria on oxygen hindered their industrial applications so that it was necessary to select oxygen-tolerant strains. Studies on their responses to oxygen might facilitate the effective utilization of bifidobacteria in industry. Oxygen-tolerant strain of Bifidobacterium longum JI-1 was able to remove 3% dissolved oxygen within 10 min whilst oxygen-sensitive strain of B. adolescentis, slime non-former, was not. The ability to remove environmental oxygen seemed to be related to the oxygen-tolerance of bifidobacteria. Mutant B. longum ADJ-1 was induced from the B. longum JI-1 under microaerobic atmosphere. There were no differences in sugar utilization pattern, NADH oxidative enzymes and cellular fatty acid compositions between them. The maximal cell density of the mutant was a little bit reduced to 81% of that of the mother strain. However, the mutant formed thick slime layer around its cell. The layer visualized with confocal scanning laser microscopy from the mutant was 6 ${\mu}{\textrm}{m}$ in diameter but that from the mother strain was only 3 ${\mu}{\textrm}{m}$. Therefore, the improved tolerances of the mutant might come from the slime layer, indicating the increase of the layer might be one of oxygen tolerance mechanisms for bifidobacteria.

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Electro-refining Characteristics of PCB-based Copper Anode for the Enrichment of Precious Metals (귀금속 농축을 위한 PCB 기반 양극동의 전해정련 특성)

  • Ahn, Nak-Kyoon;Shim, Hyun-Woo;Park, Kyung-Soo;Park, Jeung-Jin
    • Resources Recycling
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    • v.27 no.5
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    • pp.14-22
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    • 2018
  • In this study, scarp of PCB containing copper and precious metals was manufactured as an anode, and electrorefining experiments were conducted on change of $H_2SO_4$ concentration and current density. Through electrolytic refining experiments, the concentration of Cu and slime recovered from each electrode was analyzed, element behavior was confirmed, and current efficiency was also calculated. As the $H_2SO_4$ concentration was increased, the current efficiency and the purity of Cu decreased, but the precious metals in the anode slime were maximally concentrated with 2.0 M $H_2SO_4$. In addition, as the current density was increased, the current efficiency decreased and the purity of Cu showed a tendency to increase, and the precious metals in the anode slime were maximally concentrated with $300A/m^2$. As a result of the pilot scale experiments, the Au content was 8,705 mg/kg, the Ag content was 35,092 mg/kg in the anode slime. As compared with the initial content, Au was concentrated 16 times and Ag concentrated 14 times.

Cellular Slime Molds of Halla Mountain, IV Description of Polar Granule Negative Species (한라산의 세포성 점균 (IV) 극낭 음성 종의 기록)

  • 홍정수
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.9-17
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    • 1993
  • Seven dictyostelid cellular slime molds isolated from the forest soils of Mt. Halla were described and illustrated. Dictyostelium flavidum Hong et Chang and three unidentified Dictyostelium, HL-2, HL-3 and CJ-9 were compared and discussed. All the isolates were cultivated on low nutrient media, 0.1- L-P(pH6) with Escherichia coli at 20-22$^{\circ}C$. Seven unrecorded species were all polar granule (PG) negative. These were Dictyostelium crassicaule Hagiwara, D. sphaerocephalum (Oud.) Sacc. et March, D. macrocephalum Hagiwara, Yeh et Chien, D. capitatum Hagiwara, D. brefeldianum Hagiwara, D. aureum var. aureum Cavender, Worley and Raper, and D. giganteum Singh.

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Chloride Ion Penetration Resistance of Mortars including Expanded Vermiculite Immobilizing Bacteria (박테리아 흡착 팽창질석을 혼입한 모르타르의 염소이온 침투 저항성)

  • Jung, Seung-Bae;Yang, Keun-Hyeok
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2017.11a
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    • pp.151-152
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    • 2017
  • This tests examined the effectiveness of bacteria slime on the chloride ion penetration resistance of cement mortar. Test results exhibited that the chloride ion penetration depth of mortars including 5% expanded vermiculite immobilizing bacteria was 17% smaller than that of the control mortar without expanded vermiculite.

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Occurrence of Cellular Slime Molds in Turfgrass Communities (잔디(Zoysia japonica Steud)군락에서의 세포성 점균의 출현)

  • 장남기;박미아;이정은
    • Asian Journal of Turfgrass Science
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    • v.7 no.2_3
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    • pp.113-120
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    • 1993
  • Dictyostelid cellular slime molds were quantitatibly isolated from Korean lawngrass turf in Seoul National University, Korea, by the 'Clonal Isolation Technique(Cavender and Raper, 1965a)'. Total six species were found. These are Dictyostelium sphaerocephalum. D. purpuream. D. flavidam, D. crassica ale, Polysphondilium pallidum. P. violaceum. D. sphaerocephalum was the dominant species and P. violaceum, D. flaviidum, D. crassicaule were rare. Compared with other region, species diversity and species richness of Korean lawngrass turf appeared to he low. The dominant species, however, is not very common in other plant communities, so it cnay he characteristic of Korean lawngrass turf.

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Distribution of Cellular Slime Molds of Golf Courses in Kyonggi Province (경기도 골프장의 코스별 세포성 점균의 출현분포)

  • 최성영;홍정림;장남기
    • Asian Journal of Turfgrass Science
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    • v.8 no.2
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    • pp.105-110
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    • 1994
  • Dictyostelid cellular slime molds were quantitatively isolated from three golf courses in Kyonggi province. The samples were collected in tee, fairway, rough and out of bounds at each golf courses. Dictyostelium muoroides was found at rough of Seo-Seoul C.C and D. minutum at out of hounds of New Korea C.C Compared with other forests, species diversity and species richness appeared to be low. The properties of soils were investigated in three golf courses. This results were very low in water content, organic matter compared with artificial Korean lawngrsss turf in Seoul National University.

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Cellular Slime Molds in Forest Soils of Central Areas of Korea (중남부 삼림 지역에서의 세포성 점균의 출현과 분포)

  • 박미아;장남기
    • Asian Journal of Turfgrass Science
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    • v.10 no.3
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    • pp.213-230
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    • 1996
  • A study of occurrence and distribution of cellular slime molds(CSMs) in forest soils of central areas of Korea was carried out. Samples for CSMs isolation were collected from 4 study sites; Mt.Kyeryong, Mt. Taebaek, Mt. Sobaek and Mt. Sokri. In Mt. Kyeryong, six species were found. These were Dictyostelium fasciculatum, D.firmibasis, D. mucoroides, D. minutum, D. brefeldianum and Polysphondylium pallidum. The average number of species isolated at one site was 0.75, and average density(clones /g soil) was 292. The results of soil sample analyses were that the concentration of Pb was higher than any other areas. In Mt. Taebaek, seven species were found; D. fasciculatum, D.firmibasis, D. mucoroides, D. miuutum, P.pallidum. P. violaceum, P. candidum. The average number of species isolated at one site was 2.3,and average density was 1,108. Based on importance values calculated from study sites within each of three elevation ranges, several of the more widely distributed and abundant species have distribution patterns that show a response to elevation. In Mt. Sobaek, eleven species were found. These were D. fasciculatum. D.firmibasis, D. mucoroides, D. miuutum, D. brefeldianum, D. crassicaule,D. deminutivum, D. implicatum, P. pallidum. P. violaceum and P. candidum. The average number of species isolated at one site was 3, and average density was 793. Species diversity appeared to be the highest in this area. In Mt. Sokri, six species were found. These were D. fasciculatum. D. mucoroides, D. minutum, D. purpureum, P. pallidum. and P. violaceum. The average number of species isolated at one site was 2.4 and average density was 858. It was noticeable that D.purpureum were much more comrnonly found in this pinus evergreen forest. Key words: Cellular slime molds(CSMs), Importance value, Elevation, Average number of species, Average density.

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Leaching of Gold and Silver from Anode Slime with Inorganic Reagents (양극슬라임으로부터 무기침출제에 의한 금과 은의 침출)

  • Xing, Wei Dong;Lee, Ki Woog;Lee, Man Seung
    • Resources Recycling
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    • v.26 no.1
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    • pp.30-36
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    • 2017
  • Leaching experiments of anode slime were performed with several inorganic acids (HCl, $HNO_3$ and $H_2SO_4$) together with thiourea and thiosulfate solution to recover gold and silver. Gold was not dissolved at all into these inorganic acids in the absence of any oxidizing agents. At the same concentration of inorganic acid, the leaching of percentage of Ag was the highest in the sulfuric acid solution. The leaching percentage of silver increased with the increase of HCl concentration owing to the formation of $AgCl_2{^-}$. Copper, nickel and zinc except tin was almost dissolved in these inorganic acids but no tin was dissolved in nitric acid solution. Most of Au and Ag were dissolved into the mixture of sulfuric acid and thiourea solution. Thiosulfate could dissolve some silver from the anode slime but no gold was dissolved by this agent.

Regional Variations of Cellular Slime Molds Referred to Ribosomal DNA

  • Hong, Young-Bin;Kang, Kyoung-Mi;Chang, Nam-Kee
    • The Korean Journal of Ecology
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    • v.24 no.6
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    • pp.359-364
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    • 2001
  • Regional variations of Dictyostelid cellular slime molds were examined using molecular data. The intertranscribed spacer regions including the 5.8S ribosomal DNA of 2 species(D. purpureum, P. violaceum) of Cellular Slime Molds were sequenced and analyzed. Among 13 strains of D. purpureum and 12 strains of P. violaceum analyzed, each two strains were obtained from ATCC and the others were isolated from the forest soils in Korea. The sequences of the 5.8S ribosomal DNA were conserved among the strains of the same species, but unexpectedly highly variable among species. A high level of genetic diversity was found which was best resolved at the genus/species level as well as the family level by sequence data from the ITS 1 and ITS 2 regions. According to the sequence alignments by CLUSTAL X and the phylogeographic analyses by PAUP, 12 strains of P. violaceum were divided into three groups among which there were no difference of the morphological characteristics. Among 13 strains of D. purpureum, genetic variations were related to two morphological types, the temperate and subtropical type. There was no variation pattern according to geography in Korea, but there were some variations between Korea and other countries.

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