• Journal of the Korean Wood Science and Technology
• /
• 제30권3호
• /
• pp.26-33
• /
• 2002

The seven strains, Pseudomonas paucimobilis, Pseudomonas cepacia, Staphylococcus auricularis, Staphylococcus saprophyticus, Acidovorax spp., Acinetobacter calcoaceticus, and Actinobacillus capsulatus were tested with three slimicides. Most of the tested bacteria were inhibited with slimicide K (an isothiazolin based compound), even at its low concentration, except for Actinobacillus capsulatus and Staphylococcus auricularis. Both slimicides B (an organic bromine based compound) and S (aldehydes) also couldn't prevent these two strains even at their highest concentration. Five different sizes of plasmid DNA were isolated from Actinobacillus capsulatus. Staphylococcus auricularis, a gram-positive bacteria, showed the slimy substances around its cell distinctively. The results suggest that two strains, Actinobacillus capsulatus, Staphylococcus auricularis, have presumably developed a resistance to the slimicide, by plasmid DNA or slimy substance. Our findings also suggest that not only gram-negative bacteria, but also gram-positive bacteria should not be neglected

• 한국미생물·생명공학회지
• /
• 제26권6호
• /
• pp.476-482
• /
• 1998

Bifidobacterium의 효과적인 산업적 이용을 위해서는 산소에 내성이 강한 균주를 선발하는 연구뿐만 아니라, 산소 존재하에서 비피도박테리아의 내성기작을 규명하는 기초적인 연구도 필요하다. 산소에 민감한 B. adolescentis 균주와 산소내성 B. longum JI-1균주의 용존산소 제거능을 비교한 결과산소 민감성 균주는 총존산소를 전혀 제거하지 못하였으나 산소내성 균주는 10분 이내에 3%이상의 용존산소를 제거하여 총존산소 제거능과 Bifidobacterium의 산소내성과는 밀접한 관계가 있음을 알 수 있었다. 이 B. longum JI-1 균주를 미호기성 조건에서 배양하여 내산소성 돌연변이 균주인 B. longum ADJ-1 균주를 분리하였다. B. longum ADJ-1과 모균주의 특성을 당자화능, NADH 산화효소 및 세포지방산등으로 비교하였을 때 차이점을 발견하지 못했으며, 균생육은 B. longum ADJ-1이 모균주의 80% 정도를 보여 주었다. 그러나 B. longum ADJ-1는 매우 두꺼운 slime layer를 형성하였는데 confocal scanning laser microscopy에 의한 분석 결과 돌연변이 균주는직경이 약 6 $\mu\textrm{m}$에 이르는 층을 형성한 반면 모균주는 약 3 $\mu\textrm{m}$인 층을 형성하였다. 그리고 돌연변이 균주는 산소에 24시간 이상 노출되었을 경우 모균주에 비하여 더 큰 산소내성을 보여주었다. 그러므로 돌연변이 균주의 산소내성의 증가는 slime layer 차이에서 유래된 것으로 생각되며 이는 Bifidobacterium의 내산소성 기작중의 하나인 것으로 보인다.

• 자원리싸이클링
• /
• 제27권5호
• /
• pp.14-22
• /
• 2018

본 연구에서는 구리와 귀금속이 함유된 PCB 스크랩을 양극동으로 사용하여 $H_2SO_4$농도, 전류밀도 변화에 대하여 전해정련 실험을 수행하였다. 전해정련 실험을 통해 각 전극에서 회수된 Cu와 slime에 대하여 농도 분석을 하고 원소들의 거동을 확인하였으며, 전류효율도 계산하였다. $H_2SO_4$ 농도가 증가할수록 전류효율과 Cu의 순도는 감소하였지만 양극 슬라임 내에 귀금속은 2.0 M $H_2SO_4$ 조건에서 최대로 농축되었다. 또한 전류밀도가 증가할수록 전류효율은 감소하였고 Cu의 순도는 증가하는 경향을 나타내었으며 양극 슬라임 내에 귀금속은 $300A/m^2$ 조건에서 최대로 농축되었다. Pilot scale 실험 결과, 양극 슬라임에 Au 함량은 8,705 mg/kg, Ag 함량은 35,092 mg/kg으로 나타났고, 초기의 함량과 비교했을 때 Au는 약 16배, Ag는 약 14배 농축이 가능하였다.

• Journal of Plant Biology
• /
• 제36권1호
• /
• pp.9-17
• /
• 1993

Seven dictyostelid cellular slime molds isolated from the forest soils of Mt. Halla were described and illustrated. Dictyostelium flavidum Hong et Chang and three unidentified Dictyostelium, HL-2, HL-3 and CJ-9 were compared and discussed. All the isolates were cultivated on low nutrient media, 0.1- L-P(pH6) with Escherichia coli at 20-22$^{\circ}C$. Seven unrecorded species were all polar granule (PG) negative. These were Dictyostelium crassicaule Hagiwara, D. sphaerocephalum (Oud.) Sacc. et March, D. macrocephalum Hagiwara, Yeh et Chien, D. capitatum Hagiwara, D. brefeldianum Hagiwara, D. aureum var. aureum Cavender, Worley and Raper, and D. giganteum Singh.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2017년도 추계 학술논문 발표대회
• /
• pp.151-152
• /
• 2017

This tests examined the effectiveness of bacteria slime on the chloride ion penetration resistance of cement mortar. Test results exhibited that the chloride ion penetration depth of mortars including 5% expanded vermiculite immobilizing bacteria was 17% smaller than that of the control mortar without expanded vermiculite.

• 아시안잔디학회지
• /
• 제7권2_3호
• /
• pp.113-120
• /
• 1993

Dictyostelid cellular slime molds were quantitatibly isolated from Korean lawngrass turf in Seoul National University, Korea, by the 'Clonal Isolation Technique(Cavender and Raper, 1965a)'. Total six species were found. These are Dictyostelium sphaerocephalum. D. purpuream. D. flavidam, D. crassica ale, Polysphondilium pallidum. P. violaceum. D. sphaerocephalum was the dominant species and P. violaceum, D. flaviidum, D. crassicaule were rare. Compared with other region, species diversity and species richness of Korean lawngrass turf appeared to he low. The dominant species, however, is not very common in other plant communities, so it cnay he characteristic of Korean lawngrass turf.

• 아시안잔디학회지
• /
• 제8권2호
• /
• pp.105-110
• /
• 1994

Dictyostelid cellular slime molds were quantitatively isolated from three golf courses in Kyonggi province. The samples were collected in tee, fairway, rough and out of bounds at each golf courses. Dictyostelium muoroides was found at rough of Seo-Seoul C.C and D. minutum at out of hounds of New Korea C.C Compared with other forests, species diversity and species richness appeared to be low. The properties of soils were investigated in three golf courses. This results were very low in water content, organic matter compared with artificial Korean lawngrsss turf in Seoul National University.

• 아시안잔디학회지
• /
• 제10권3호
• /
• pp.213-230
• /
• 1996

A study of occurrence and distribution of cellular slime molds(CSMs) in forest soils of central areas of Korea was carried out. Samples for CSMs isolation were collected from 4 study sites; Mt.Kyeryong, Mt. Taebaek, Mt. Sobaek and Mt. Sokri. In Mt. Kyeryong, six species were found. These were Dictyostelium fasciculatum, D.firmibasis, D. mucoroides, D. minutum, D. brefeldianum and Polysphondylium pallidum. The average number of species isolated at one site was 0.75, and average density(clones /g soil) was 292. The results of soil sample analyses were that the concentration of Pb was higher than any other areas. In Mt. Taebaek, seven species were found; D. fasciculatum, D.firmibasis, D. mucoroides, D. miuutum, P.pallidum. P. violaceum, P. candidum. The average number of species isolated at one site was 2.3,and average density was 1,108. Based on importance values calculated from study sites within each of three elevation ranges, several of the more widely distributed and abundant species have distribution patterns that show a response to elevation. In Mt. Sobaek, eleven species were found. These were D. fasciculatum. D.firmibasis, D. mucoroides, D. miuutum, D. brefeldianum, D. crassicaule,D. deminutivum, D. implicatum, P. pallidum. P. violaceum and P. candidum. The average number of species isolated at one site was 3, and average density was 793. Species diversity appeared to be the highest in this area. In Mt. Sokri, six species were found. These were D. fasciculatum. D. mucoroides, D. minutum, D. purpureum, P. pallidum. and P. violaceum. The average number of species isolated at one site was 2.4 and average density was 858. It was noticeable that D.purpureum were much more comrnonly found in this pinus evergreen forest. Key words: Cellular slime molds(CSMs), Importance value, Elevation, Average number of species, Average density.

• 자원리싸이클링
• /
• 제26권1호
• /
• pp.30-36
• /
• 2017

양극흙에 함유된 금과 은을 회수하기 위해 무기산(염산, 질산, 황산)과 thiourea 및 thiosulfate에 의한 침출실험을 수행했다. 산화제를 첨가하지 않은 조건에서 금은 무기산용액에 전혀 용해되지 않았다. 3종류의 무기산의 농도가 동일한 조건에서 황산용액에서 은의 침출율이 가장 높았으며, 염산의 농도가 증가함에 따라 $AgCl_2{^-}$의 형성에 따라 은의 침출율이 증가하였다. 한편 주석은 질산용액에 전혀 용해되지 않았으나, 구리, 니켈과 아연은 본 실험조건에서 모두 용해되었다. 황산과 thiourea의 혼합용액에서 금과 은은 모두 용해되었다. Thiosulfate용액에 은의 일부가 용해되었으나, 금은 전혀 용해되지 않았다.

• The Korean Journal of Ecology
• /
• 제24권6호
• /
• pp.359-364
• /
• 2001

Regional variations of Dictyostelid cellular slime molds were examined using molecular data. The intertranscribed spacer regions including the 5.8S ribosomal DNA of 2 species(D. purpureum, P. violaceum) of Cellular Slime Molds were sequenced and analyzed. Among 13 strains of D. purpureum and 12 strains of P. violaceum analyzed, each two strains were obtained from ATCC and the others were isolated from the forest soils in Korea. The sequences of the 5.8S ribosomal DNA were conserved among the strains of the same species, but unexpectedly highly variable among species. A high level of genetic diversity was found which was best resolved at the genus/species level as well as the family level by sequence data from the ITS 1 and ITS 2 regions. According to the sequence alignments by CLUSTAL X and the phylogeographic analyses by PAUP, 12 strains of P. violaceum were divided into three groups among which there were no difference of the morphological characteristics. Among 13 strains of D. purpureum, genetic variations were related to two morphological types, the temperate and subtropical type. There was no variation pattern according to geography in Korea, but there were some variations between Korea and other countries.