• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.1 s.32
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• pp.130-144
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• 2007

UV-irradiated skin shows acutely erythema, edema, pigmantation (sunbum) and chronically coarse wrinkling, roughness, dryness, laxity (photoaging). Jawoongo(紫雲膏, JW) is clinically useful external application and effective bum, sunburn, wound and symptom of dryness(燥症) in skin disease. In this experiment, we examined if JW could cure the UVB-mediated acute skin damages, inhibit UVB-mediated oxidative stress and inflammation of skin, and block the photoaging. In vivo test, we found that JW could effectively cure the UVB-mediated acute skin damages(erythema, edema, angiogenesis, hyperplasia, infiltration of lymphocytes) and inhibit expression of HSP70, CYP1A1 and p53. We also found that JW could repair destruction of collagen fiber and inhibit activation of MMP-9, and inhibit expression of $NF-{\kappa}B$ p65, iNOS, hyperplasia of keratynocyte. In vitro test, we found that JW could inhibit expression of IKK, iNOS mRNA, and production of NO. These findings shows that JW could cure the UVB-mediated acute skin damages, inhibit UVB-mediated oxidative stress and inflammation of skin, and block photoaging.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.31 no.3
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• pp.222-228
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• 2009

Objectives: This study is aimed to compare the wound healing processes between conventional scapel wound and Er,Cr:YSGG (Erbium,Chromium, Yttrium, Scandium, Gallium, Garnet) laser wound using experimental animals. Experimental Design: Two types of wounds were made by linear and round incisions using scalpel and Er,Cr:YSGG laser, respectively, on the thigh of Sprague-Dawley rats. Sprague-Dawley rats were serially sacrified as follows: post operative 12, 24, 48 hours, and 3, 7, 14 days. The skin wounds were grossly and microscopically analyzed during the healing period. Result: The Er,Cr:YSGG laser incision showed better wound healing for the linear incision experiment than the scapel incision. Whereas the scapel incision showed better wound healing for the round incision experiment than the Er,Cr:YSGG linear incision. As the Er,Cr:YSGG laser damage in the round incision experiment could be much increased compared with the round incision by scapel. So, the round incisions by the Er,Cr:YSGG laser were resulted in the poor wound healing compared with those by the scapel. Conclusion: The Er,Cr:YSGG laser is more favorable for the fast linear incision, while the scapel is more favorable for the modified round incision.

• Archives of Plastic Surgery
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• v.35 no.2
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• pp.121-126
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• 2008

Purpose: Poly-N-acetyl glucosamine(PGlcNAc) nanofiber-based materials, produced by a marine microalga, have been characterized as effective hemostatic and angiogenic agents. The similarity between PGlcNAc patch and the natural extracellular matrix allows it to support new healthy tissue growth in an injured area and to encourage fluid absorption. In this study, we hypothesized that a poly-N-acetyl glucosamine fiber patch(PGlcNAc patch) may enhance wound healing in the db/db mouse. Methods: PGlcNAc patches were applied on one square centimeter, full-thickness, skin wounds in the db/db mouse model. Wounds(n=15 per group) were dressed with a PGlcNAc nanofiber patch for 1 hour(1 h), 24 hours(24 h) or left untreated(NT). After the application time, patches were removed and wounds were allowed to heal spontaneously. The rate of wound closure was evaluated by digital analysis of unclosed wound area in course of time. At day 10, wounds(n=7 per group) were harvested and quantified with immunohistochemical markers of proliferation(Ki-67) and vascularization (platelet endothelial cell adhesion molecule, PECAM-1). Results: Wounds dressed with PGlcNAc patches for 1 hour closed faster than control wounds, reaching 90% closure in 16.6 days, nine days faster than untreated wounds. Granulation tissue showed higher levels of proliferation and vascularization following 1 h treatment than the 24 h and NT groups. In addition to its hemostatic properties, the PGlcNAc material also appears to accelerate wound closure in healing-impaired genetically diabetic mice. Conclusion: This material, with its combination of hemostatic and wound healing properties, has the potential to be effective agent for the treatment of complicated wounds.

• Archives of Plastic Surgery
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• v.36 no.5
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• pp.519-524
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• 2009

Purpose: This study was conducted to establish the most effective method of cell therapy by comparing and analyzing the level of wound healing after various cell delivery methods. Methods: Human mesenchymal stem cells were administered using 5 different methods on full thickness skin defects which were deliberately created on the back of 4 - week old mice using a 8 mm punch. Different modes of administration, cell suspension, local injection, collagen GAG matrix seeding, fibrin, and hydrogel mix methods were used. In each experiment group, $4{\times}105$ mesenchymal stem cells were administered according to 5 deferent methods, and were not for the corresponding control group. Results: The wound healing rate was fastest in the local injection group. The wound healing rate was relatively slow in the collagen matrix group, however, the number of blood vessels or VEGF increased most in this group. Conclusion: For rapid wound healing through wound contraction, it is advantageous to administer MSC by the local injection method. For the healing process of a wide area, such as a burn, the seeding of cells to collagen matrix is thought to be effective.

• The Journal of the Korean dental association
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• v.22 no.1 s.176
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• pp.49-55
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• 1984

The objective of this study was to observe the healing of skin and mucosal grafts, comparing with the control sites. Six adult dogs were divided into two groups of three. Mucosal defects were created in all four quadrants, and the left side was used as a control. In the Group I animals, a split-thickness skin from the chest was grafted, and in the group II animals, a split-thickness mucosa from the buccal cheek was grafted into the mouth. Gross and microscopic examination was done at 3, 7, 14, 28, 42 days. The results were as follows. 1. Wound epithelization was accelerated in the supraperiosteal grafts. 2. After healing, the grafted skin was unnatural to the oral cavity, showing grayish-white color, hair, and sebaceous gland. But the mucosal grafts could not be discriminated from the adjacent mucosa. 3. the grafting on the cortical bone failed.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.45 no.6
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• pp.374-376
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• 2019

Last week, after our receiving online journal regarding Journal of the Korean Association of Oral and Maxillofacial Surgeons, we found a recently published original article by Alawode et al., entitled "A comparative study of immediate wound healing complications following cleft lip repair using either absorbable or non-absorbable skin sutures". Although this clinical article was well written and provided a great deal of information regarding the suture materials in the cleft lip repair, I would like to add a few additional comments based on the importance of skin suture during cheiloplasties in the primary cleft lip or secondary revision patients with representative figures.

• Archives of Plastic Surgery
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• v.49 no.3
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• pp.405-412
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• 2022

Although modern medicine has made great strides in the management of burn injuries, associated complications such as pain, infection, dyspigmentation, and scarring have yet to be fully dealt with. Although skin grafting and meshing are routinely performed on burn patients, this method poses a risk for adverse effects. Activated autologous platelet-rich plasma (aaPRP), which is increasingly used in the field of plastic surgery, contains growth factors beneficial for wound regeneration. Seven cases of burns with varying severity and conditions that were treated with intralesional subcutaneous injection and intravenous aaPRP are presented and discussed herein. This case series indicates that subcutaneous and intravenous aaPRP is a safe procedure with the potential to be an alternative when skin grafting cannot be done or as an adjunct treatment to skin grafting.

• Archives of Plastic Surgery
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• v.48 no.6
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• pp.641-645
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• 2021

A 3,480 g male neonate showed tachypnea symptom with a serum ionized calcium level of 0.66 mmol/L by routine clinical analysis. He was injected calcium gluconate intravenously through femoral vein catheter to treat the hypocalcemia. On second day after the injection, he started to show erythema in the flank area. The lesion became firm and changed into whitish crust consist of small crystals. Abdominal X-ray and ultrasonography showed the accumulation of calcium deposit in the subcutaneous layer of the lesion. Surgical debridement was performed to remove the crust with calcium deposit and acellular fish skin graft rich in omega-3 (Kerecis) was applied to the defect site for secondary intention of the defect wound. After 2 months, the skin and soft tissue defect were fully covered with healthy normal skin without depression or contracture. This report is a first case of iatrogenic calcinosis cutis without extravasation symptom.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.2 no.1
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• pp.17-31
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• 1989

This study was under taken to investigate the healing of Sa Gun Ja Tang extract (SE) and Sa Gun Ja Tang added Radix Astragali extract (SHE) on the artificial wound on rabbit skin. The granulation tissues were observed by microscope at the five day interval for twenty days. In order to understand the healing mechanism chick embryo culture was carried out in the presence of the extracts and the growth ratio and fusion index were counted. The results obtained were as follows: 1. The healing power of SE treated group on wound was more significant than the control group. 2. The healing power on wound was more effective at the SHE than the SE. 3. The growth ratio and fusion index were higher at the SHE treated groups than the SE treated groups. From the above result, SHE was more effective than the SE on wound healing activity by the mechanism of stimulating the cell growth ratio and fusion index.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.8
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• pp.755-760
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• 2015

For proper wound healing, dermal contraction and remodeling are critical; during the natural healing process, differentiated fibroblasts called "myofibroblasts" typically undertake these functions. For severe wounds, however, a critical mass of dermal matrix and fibroblasts are lost, making self-regeneration impossible. To overcome this impairment, synthetic wound patches with embedded functional cells can be used to promote healing. In this study, we developed a polydioxanone (PDO)-based cell-embedded sheet on which dermal fibroblasts were cultured and induced for differentiation into myofibroblasts, whereby the following combinatorial physicochemical stimuli were also applied: aligned topology, electric field (EF), and growth factor. The results show that both the aligned topology and EF synergistically enhanced the expression of alpha smooth-muscle actin (${\alpha}$-SMA), a key myofibroblast marker. Our proof-of-concept (POC) experiments demonstrated the potential applicability of a myofibroblast-embedded PDO sheet as a wound patch.