Browse > Article
http://dx.doi.org/10.7736/KSPE.2015.32.8.755

Combinatorial Physical Stimulation and Synergistically-Enhanced Fibroblast Differentiation for Skin Regeneration  

Ko, Ung Hyun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Hong, Jungwoo (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Shin, Hyunjun (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Kim, Cheol Woong (Triple-C Medical Corporation)
Shin, Jennifer H. (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
Publication Information
Journal of the Korean Society for Precision Engineering / v.32, no.8, 2015 , pp. 755-760 More about this Journal
Abstract
For proper wound healing, dermal contraction and remodeling are critical; during the natural healing process, differentiated fibroblasts called "myofibroblasts" typically undertake these functions. For severe wounds, however, a critical mass of dermal matrix and fibroblasts are lost, making self-regeneration impossible. To overcome this impairment, synthetic wound patches with embedded functional cells can be used to promote healing. In this study, we developed a polydioxanone (PDO)-based cell-embedded sheet on which dermal fibroblasts were cultured and induced for differentiation into myofibroblasts, whereby the following combinatorial physicochemical stimuli were also applied: aligned topology, electric field (EF), and growth factor. The results show that both the aligned topology and EF synergistically enhanced the expression of alpha smooth-muscle actin (${\alpha}$-SMA), a key myofibroblast marker. Our proof-of-concept (POC) experiments demonstrated the potential applicability of a myofibroblast-embedded PDO sheet as a wound patch.
Keywords
Wound patch; Fibroblast; Electrospinning; Polydioxanone (PDO); Electrical stimulation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Park, S. C., Oh, S. H., and Lee, J. H., "Alginate/Aloe-Containing Poly (Vinyl Alcohol) Hydrogel Patch as a Wound Dressing," Biomaterials Research, Vol. 13, No. 4, pp. 138-143, 2009.
2 Murphy, P. S. and Evans, G. R., "Advances in Wound Healing: A Review of Current Wound Healing Products," Plastic Surgery International, Vol. 2012, Article ID. 190436, 2012.
3 Vikatmaa, P., Juutilainen, V., Kuukasjarvi, P., and Malmivaara, A., "Negative Pressure Wound Therapy: A Systematic Review on Effectiveness and Safety," European Journal of Vascular and Endovascular Surgery, Vol. 36, No. 4, pp. 438-448, 2008.   DOI
4 Cianfarani, F., Zambruno, G., Brogelli, L., Sera, F., Lacal, P. M., et al., "Placenta Growth Factor in Diabetic Wound Healing," The American Journal of Pathology, Vol. 169, No. 4, pp. 1367-1182, 2006.
5 Augustine, R., Kalarikkal, N., and Thomas, S., "Advancement of Wound Care from Grafts to Bioengineered Smart Skin Substitutes," Progress in Biomaterials, Vol. 3, No. 2-4, pp. 103-113, 2014.   DOI
6 Boateng, J. S., Matthews, K. H., Stevens, H. N., and Eccleston, G. M., "Wound Healing Dressings and Drug Delivery Systems: A Review," Journal of Pharmaceutical Sciences, Vol. 97, No. 8, pp. 2892-2923, 2008.   DOI
7 Sun, G., Zhang, X., Shen, Y.-I., Sebastian, R., Dickinson, L. E., et al., "Dextran Hydrogel Scaffolds Enhance Angiogenic Responses and Promote Complete Skin Regeneration during Burn Wound Healing," Proc. of the National Academy of Sciences, Vol. 108, No. 52, pp. 20976-20981, 2011.   DOI
8 Balint, R., Cassidy, N. J., and Cartmell, S. H., "Electrical Stimulation: A Novel Tool for Tissue Engineering," Tissue Engineering Part B: Reviews, Vol. 19, No. 1, pp. 48-57, 2012.   DOI
9 Evans, R. A., Tian, Y. C., Steadman, R., and Phillips, A. O., "Tgf-${\beta}1$-Mediated Fibroblast-Myofibroblast Terminal Differentiation-The Role of Smad Proteins," Experimental Cell Research, Vol. 282, No. 2, pp. 90-100, 2003.   DOI
10 Tomasek, J. J., Gabbiani, G., Hinz, B., Chaponnier, C., and Brown, R. A., "Myofibroblasts and Mechano-Regulation of Connective Tissue Remodelling," Nature Reviews Molecular Cell Biology, Vol. 3, No. 5, pp. 349-363, 2002.   DOI
11 Park, S. H., Park, J. H., Lee, H. J., and Lee, N. K., "Current Status of Biomedical Applications Using 3D Printing Technology," J. Korean Soc. Precis. Eng., Vol. 31, No. 12, pp. 1067-1076, 2014.   DOI
12 Park, S.-H., Koh, U. H., Kim, M., Yang, D.-Y., Suh, K.-Y., et al., "Hierarchical Multilayer Assembly of an Ordered Nanofibrous Scaffold via Thermal Fusion Bonding," Biofabrication, Vol. 6, No. 2, Paper No. 024107, 2014.
13 Guo, A., Song, B., Reid, B., Gu, Y., Forrester, J. V., et al., "Effects of Physiological Electric Fields on Migration of Human Dermal Fibroblasts," Journal of Investigative Dermatology, Vol. 130, No. 9, pp. 2320-2327, 2010.   DOI
14 Song, S., Han, H., Ko, U. H., Kim, J., and Shin, J. H., "Collaborative Effects of Electric Field and Fluid Shear Stress on Fibroblast Migration," Lab on a Chip, Vol. 13, No. 8, pp. 1602-1611, 2013.   DOI
15 Liao, I.-C., Liu, J. B., Bursac, N., and Leong, K. W., "Effect of Electromechanical Stimulation on the Maturation of Myotubes on Aligned Electrospun Fibers," Cellular and Molecular Bioengineering, Vol. 1, No. 2-3, pp. 133-145, 2008.   DOI