• Molecules and Cells
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• 제44권7호
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• pp.500-516
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• 2021

Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. Recent developments in mass spectrometry-based phosphoproteomics have led to the identification of thousands of differentially phosphorylated proteins and their phosphorylation sites. However, functional studies of these differentially phosphorylated proteins have not been conducted in a large-scale or high-throughput manner due to a lack of methods capable of revealing the functional relevance of each phosphorylation site. In this study, an integrated approach combining quantitative phosphoproteomics and cell-based functional screening using phosphorylation competition peptides was developed. A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. Cell-based functional screening against 18 selected phosphorylation sites identified three phosphorylation sites (Ser-98, Ser-179 of Ldb3, and Ser-1146 of palladin) displaying near-complete inhibition of cardiac hypertrophic growth of NRVMs. Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. Our integrated approach can be used to identify functionally important phosphorylation sites among differentially phosphorylated sites, and unlike conventional approaches, it is easily applicable for large-scale and/or high-throughput analyses.

• 대한토목학회논문집
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• 제41권6호
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• pp.745-750
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• 2021

최근 미세먼지에 대한 국민들의 불안감이 증가함에 비산먼지에 대한 관심이 증가하고 있다. 비산먼지는 도로재비산먼지 다음으로 건설현장에서 많이 발생하는데 이 중 대부분을 차지하는 소규모 현장은 비산먼지 관련 규정이 없어 제대로 된 관리가 이뤄지지 않고 있는 실정이다. 이에 본 연구에서는 소규모 현장 비산먼지 관리 규정 수립에 기여하고 비산먼지 저감방안을 마련하고자 서울시 소규모 현장에서 발생하는 비산먼지를 측정 및 분석을 진행했다. 소규모 현장에서 발생하는 비산먼지의 시간별 변동을 확인했고 담장과 난간 뒤에서 측정했을 때 상대적으로 농도가 낮은 것을 통해 비산먼지 저감방안으로 간이 펜스 설치 방안을 제시했다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2011년도 춘계학술발표대회
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• pp.544-546
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• 2011

Due to the explosive growth of online social network users, large numbers of users discover these social network sites are a place where they can be able to spend their spare time, share feelings, ideas freely, and to search for new friends or partners. These web sites give an opportunity for its users to socialize with new people and to keep in touch or reconnect with current or old friends and families across disperse continents via these web sites, which traditionally replace the traditional methods. These social network web sites need careful investigations and findings on the usability for effective interactivity and more usability. However, little research might have previously invested on the usability of these on social network web sites. Therefore, we propose a new framework to study the usability of these social network sites. We namely call our framework "Interactivity". This framework will enable us to assess the usability of the social network sites. It will provide an overview of the user's behavior while interacting in these social network web sites. Measurement of the interactivity will be measured using Camtasia software. This software will entirely capture the interactivity of users including the screen and the movements, which the screen and the motion of the user action will undergo to analysis at the end of our research.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권3호
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• pp.124-138
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• 2024

The International Union for Conservation of Nature and the Korean Red Data Book both categorize the dragon swallowtail Sericinus montela Grey, 1852 (Lepidoptera: Papilionidae) as a vulnerable insect. In South Korea, the main reason for this categorization is habitat destruction and deterioration, along with limited distribution mainly to the eastern region of South Korea, particularly to Gangwon-do and Gyeongsangbuk-do. Considering much of the information is older than ten years the renewed survey to estimate distributional stability is required, particularly under global warming. In addition, the population genetic diversity and isolation of S. montela, which is important information when seeking to evaluate its vulnerability, is not yet known. In this study, we visited 38 habitable sites, consisted of 15 sites with the previous record for the sightings of the species and 23 sites without such record. Moreover, nuclear ribosomal internal transcribed spacer region was sequenced for 56 individuals collected from seven sites. We observed S. montela at 20 sites, consisted of 11 recorded and 9 new sites in seven provinces, excluding Jeollanam-do and Jeju-do. Finding of the species in 9 new sites and location of the 4 in western region, which has a rare previous record are promising. However, failure to observe in 4 recorded sites and an extremely low observed number in new sites (5.3 vs. 17.6 individuals per site on average) is not optimistic. Population genetic data partially corroborate with the field observation data, showing that the populations in western region have lower genetic diversity and intermittent genetic isolation compared to those in eastern region. Taken these together, we suggest that S. montela should be remained as a vulnerable insect. However, more extensive field monitoring and co-dominant molecular data are required to verify this conclusion.

• 디지털융복합연구
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• 제12권1호
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• pp.365-370
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• 2014

웹의 역할이 커져가고 있는 정보화 사회에서 장애인을 위한 웹 접근성의 중요도 또한 커져가고 있다. 본 논문에서는 장애인 관련 웹 사이트들의 접근성 실태를 알아보고자 학교 웹 사이트들과 비교 분석하여 보았다. 웹 접근성 평가 실험 결과 장애인 관련 웹 사이트들의 접근성이 일반 학교의 웹 접근성보다 낮게 평가 되었다. 본 연구 결과는 일반 웹 사이트들에 비하여 장애인 관련 웹 사이트들의 웹 접근성 향상 노력이 더욱 필요함을 보여준다.

• 한국대기환경학회지
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• 제13권6호
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• pp.439-450
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• 1997

Atmospheric fine particles $(PM_{2.5})$ were collected at the background sites, Kangwha, Taean, and Kosan and characterized to understand their behaviors at the sites. Daily samples of $PM_{2.5}$ mass were measured and ionic species, carbonaceous species, and gaseous species were analyzed. Four-day backward trajectory analysis was also carried out. The mean concentrations of anthropogenic species were highest at Kangwha among three sites, while contributions from sea salts wree highest at Taean during the measurement period due to higher wind speed at Taean. Major chemical components in fine particles were sulfate, organic carbon, nitrate, and ammoniu. Most of the non-sea-salt (nss) sulfates in $PM_{2.5}$ might be present as ammonium sulfates at these sites. Most air parcels arriving at Kangwha and Taean were from northern China. Therefore, both sites were thought to be affected by the same air parcel. At Kosan, during the measurement period, air parcels were from either northern China or sourthern China. The nss sulfate concentration in the air parcels from southern China was higher, while the nss calcium, nitrate, and ammonium concentrations were higher when the air parcels were from northern China.

• 가정과삶의질연구
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• 제26권1호
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• pp.15-29
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• 2008

The study examined the characteristics of internet shopping mall sites including on-line market places and integrated internet stores from a perspective of consumer protection. Specifically, the study investigated 1) whether sufficient information is provided on the internet shopping mall sites, 2) whether shopping mall sites abide by internet advertising principles, and 3) specific examples of consumer misleading potentialities from internet shopping sites. Further, on-line market places and integrated internet stores were compared based on their informativeness and potential consumer misleading factors. Total 50 shopping mall sites were analyzed for examining the types of information provided and consumer misleading factors. To verify study questions, descriptive statistics were used. For comparison between on-line market places and integrated internet stores, Chi-square test and independent-sample t-test were employed. The results of this study were as the following: First, not a few of the internet shopping mall sites did not provided important information such as safety and warrantees. Second, important disclosures were not noticeable on the web pages and distracting factors were frequently used. Third, integrated internet stores were desirable compared to on-line marketplaces based on informativeness and consumer misleading potentialities. As the result of this study, internet shopping malls need to advised to provide sufficient information and reduce potential misleading factors.

• 한국토양비료학회지
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• 제47권6호
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• pp.506-509
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• 2014

This study was conducted to identify transition characteristics of mercury in several selected medicinal plants and to find the appropriate management for production of safety food. Cultivated soils and medicinal plants were collected at 29 sites for Angelica gigas (Korean angelica root), 68 sites for Platycodon grandiflorum (Balloon flower), 35 sites for codonopsis lanceolata (Deoduck), 36 sites for Dioscorea batatas (Chinese yam), 32 sites for Rehmannia glutinosa (Foxglove), 16 sites for Cnidium officinale makino (cnidium), and 26 sites for Astragalus membranaceus (milk vetch root) during the harvest season of 2013. Mercury in the soils and medicinal roots were analyzed with a Direct Mercury Analyzer. Average content of mercury in soils cultivated medicinal plants was $0.023mg\;kg^{-1}$ (range: from 0.003 to $0.074mg\;kg^{-1}$) and average content of mercury in medicinal plants was $0.003mg\;kg^{-1}$ (range: from 0.001 to $0.011mg\;kg^{-1}$), indicating that mercury in the surveyed soils and medicinal plants were not exceeded the Korean regulation.

• 한국안전학회지
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• 제28권5호
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• pp.27-34
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• 2013

Almost accidents in construction sites are occurred by unsafe work conditions and failure making safe environment. As recent construction projects become more complex, various and diversified, So the potential of construction-related accidents is increased as well. Comparing the frequency of construction-related accidents in large-sized construction sites, small-sized construction sites are more vulnerable because of lack of workforce and less effective technology system. Furthermore, the absence of safety committee, poor financial condition leads to lack of safety education, installation of safety facilities, and provision of individual protective equipment, which indicates difficulties handling even basic safety management. Therefore, in order to lower the hazard rate at small-Sized construction sites, it is necessary to proceed with more structural and thorough actions for preventing accidents. In other words, it is necessary to analyze the causes of the accidents at small-sized construction sites, set criteria for safety management, and suggest safety management items according to work classification by evaluating the relative importance of the items.

• 한국대기환경학회지
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• 제16권4호
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• pp.415-420
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• 2000

The daily mean concentrations of PM2.5 mass ionic species carbonaceous species and HNO3, HCl, and NH3 were analyzed at three background sites in Korea : Kangwha, Kosan, and Dongbaik during two intensive field studies between December 1996 and January 1997, Four-day backward trajectory analysis was also carried out. The mean concentrations of anthropogenic species were higher at Kangwha and Dongbaik than Kosan. At these two sites themean concentrations of nitrate and ammonium/ammonia were higher than those of Kosan. It was suggested that these two sites be considered as the background sites of the Seoul Metropolitan Area and Pusan respectively. Major chemical components in fine particles were sulfate organic carbon nitrate and ammonium for all the sites. More than 90% of sulfate were non-sea-salt(nss) sulfates and most of the nss sulfate in PM2.5 might be present as ammonium sulfates at all sites. Most of air parcels arriving a Kangwha and Dongbaik were from the northern China. At Kosan during the measurement period half of air parcels were from the northern China and the other half from the southern China. At Kosan the concentrations of anthropogenic air pollutants originated from the southern China were higher than those typically observed from the northern China.