• Title/Summary/Keyword: site characteristics

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Active-Site Mutants of Human Glutathione S-Transferase P1-1: Effects of the Mutations on Substrate Specificity and Inhibition Characteristics

  • Park, Hee-Joong;Yoon, Suck-Young;Kong, Kwang-Hoon
    • BMB Reports
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    • v.31 no.4
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    • pp.399-404
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    • 1998
  • In order to gain further insight on the relationship between structure and function of glutathione S-transferase (GST), the six active-site mutants, R13T, K44T, Q51A, Q64A, S65A, and D98A, of human GST P1-1 were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. The active-site mutants showed marked differences in substrate specificity. The substitution of Gln51 with threonine resulted in a drastic decrease in the specific activities to <10% of the wild-type value. The substitution of Arg13 with threonine resulted in more decreased specific activity toward cumene hydroperoxide and in the $I_{50}$ values of S-(2,4-dinitrophenyl) glutathione and benanstatin A. These results suggest that the substitution of Arg13 with threonine changes the conformation of the active site to increase the affinity for the product or electrophilic substrate. Lys44 seems to be in the vicinity of the H-site of hGST P1-1 or may contribute to some extents to the electrophile binding.

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A Case Study on Simplified Assessment Method for Site Selection of the Waste Treatment Facilities in Korea (폐기물 처리시설 입지선정의 효율화 방안에 관한 연구 - 여주군 폐기물 매립지 입지선정 사례를 중심으로 -)

  • Lee, Mu Choon;Koo, Ja Kon;Kim, Ki Cheol;Kwon, Yeon Jeong
    • Journal of Environmental Impact Assessment
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    • v.8 no.1
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    • pp.71-79
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    • 1999
  • The comparative evaluation is the most effective method for site selection because the selection of waste treatment facility is to determine the optimum site out of limited candidate sites. This study adopted the ordinal scale evaluation, one of methods of comparative evaluation. The ordinal scale evaluation aims to determine the investigating items referring to the character of sites, to determine the importance factors for investigating items, and to determine the optimum site according to the quantitative evaluation. As a result of this study, the defects of the former reports on the environmental characteristics, such as obscurity of meaning and subjective statement, were reduced by the ordinal scale evaluation which is one of the quantitative evaluation methods. This ordinal scale evaluation method has some valuable advantages, such as, to be able to consider the cost-effect efficiency, to consider the objectiveness and the clearness of the reports on the environmental characteristics. Therefore the reducement of social complications about site selection of the indisposed facilities could be expected by this study.

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Model development for site selection considering the characteristics of LNG receiving terminal (LNG 특성을 고려한 저장기지 입지선정 모델 개발)

  • Jeong, Nam Hoon;Liu, An Qi;Hwang, Geon Wook;Jang, Woosik;Han, Seung Heon
    • Korean Journal of Construction Engineering and Management
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    • v.16 no.1
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    • pp.82-91
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    • 2015
  • Recently, due to the increasing concern of environmental factors and low carbon usage, the use of natural gas has been inclining steadily. In order to meet the growing demand of natural gas, government have established strategies to secure the sufficient amount of gas that is mainly used by industries, power generation and residential use by constructing additional receiving terminals for Liquid Natural Gas (LNG). In the process of selecting the optimal site for the terminals, the characteristics of the terminals are not considered where the decision making is done through internal meetings or outsourcing. In respect to site selection, researches are done to derive the factors that are considered for optimal site selection. However, there have not yet been researches in creating a systematic model for analyzing the optimal site selection. To this aim, the paper aims to propose a model for site selection of LNG receiving terminals that considers the characteristics of the terminal construction. Total of 47 factors considered in site selection is derived through interviews with experts and analyzing the previous cases of site selection by various firms. Furthermore, the derived 47 factors are used for the survey for the previous LNG terminals in PT, IC, TY, SC and BR areas where the survey data is analyzed by factor analysis and multiple regression models to depict the optimal site. By applying the model for site selection, practitioners are able to make decisions for site selection in a systematic approach for new candidates of sites.

The Sequential Effects of Site and Customer Characteristics of Internet Shopping Malls on Perceived Risks and Purchase Intention (인터넷 쇼핑몰의 사이트 특성과 사용자의 특성이 지각된 위험과 구매의도에 미치는 영향)

  • Jeong, Seung-Ryul;Kang, Young-Shin;Lee, Choon-Yeul
    • The KIPS Transactions:PartD
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    • v.14D no.4 s.114
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    • pp.407-420
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    • 2007
  • The purpose of this study is to examine the effects of the site characteristics and the customer characteristics of Internet shopping malls on perceived risk and purchase intention. In order to fulfill this objective, we employ the survey methodology to gain data and analyze them using a structural equation model. The results of this study show that both the site characteristics and the customer characteristics of Internet shopping malls did not have a direct effect on purchase intention. Instead, perceived risk has effects on purchase intention. This implies that perceived risk is playing an important role in e-commerce.

Sewage Treatment Characteristics and Efficiencies of Absorbent Biofilter Systems (흡수성 바이오필터 시스템의 오수처리 특성 및 효율)

  • Cheon, Gi-Seol;Kwun, Soon-Kuk;Kim, Song-Bae
    • Journal of The Korean Society of Agricultural Engineers
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    • v.46 no.5
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    • pp.131-139
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    • 2004
  • In this study, on-site sewage treatment tests were conducted using the Absorbent Biofilter System (ABS) under different hydraulic loading rates to examine its treatment characteristics and efficiencies and to determine its feasibility as a small on-site sewage treatment system in a rural area. Results showed that the removal rates of BOD and SS were satisfactory at hydraulic loading rates of 100~150 cm/day, meeting the Korean effluent water quality standards for the riparian zone (10 mg/L). In the case of nutrients (N, P), however, the system did not perform well, necessitating further improvement for nutrient removal. A comparative analysis indicated that as a small on-site sewage treatment system, the ABS would be more suitable than other treatment systems in terms of performance stability, maintenance requirement, and cost-effectiveness and could be applied as an alternative treatment system in Korean rural areas.

산악지역 내 LNAPL 오염의 개념모델 정립을 위한 사례연구

  • Kang, U-Jae;Gong, Jun;Jeon, Jin-Oh;Lee, Sang-Bong;Hwang, Jong-Sik;Bae, U-Geun
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.04a
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    • pp.85-88
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    • 2001
  • Since mountainous area has access restrictions for field work, assessors need to establish a conceptual model of the contamination prior to the field investigation. In this study we established a conceptual model of the contamination based on site inspection and geological survey, followed by the field investigation for the petroleum spill site. In the conceptual model, we estimated that tile contamination should have spread by groundwater and topographical characteristics within the top soil layer. The spread of contamination through rock was not considered in the conceptual model due to impermeable characteristics of metasyenite. The contaminated environmental media of the petroleum spill site include soil and groundwater. According to the analysis result of the contamination, the volume of contaminated soil is estimated approximately 4, 150 cubic meters (7, 055 ton) with most contaminants existing along the groundwater flow within top soil layer.

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Analysis on the Site Characteristics for the Restoration of Sangrim Woodlands in Hamyang-Gun, Korea (함양 상림 복원을 위한 입지특성 분석)

  • Park, Jae-Hyeon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.8 no.1
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    • pp.1-9
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    • 2005
  • This study was conducted to establish a management plan for the Sangrim Woodlands restoration by analyzing the site characteristics of the Sangrim Woodlands Natural Monument(Natural Monument 154) in Hamyang-Gun, Gyoungsangnam-Do, Korea. Site preparation to enhance soil aeration should be applied because soil bulk density in all study sites was higher than soil compaction of natural forest soil area. Herbaceous plants could be introduced to hard soil strength for restoration of areas compacted by visitors. Also, visitors around forest areas should be restricted to enhance natural soil restoration. Soil pH in the Sangrim Woodlands was between 4.18 and 4.90. The values were lower than pH 5.34 of Korean forest soil originated from metamorphic parent materials. Lime fertilizer could be applied to reduce soil acidification in the woodlands. Short and long-term management plans such as periodical fertilizations to improve plant growth should be established to restore the Sangrim Woodlands which have high soil compaction, low soil pH and organic matter content.

Current Techniques for Residential Site Design -The Case of Onohara District, Japan- (주거환경을 생각한 주택단지 조성기법의 실태 -일본 소야원지구의 사례 연구-)

  • 김한수
    • Journal of the Korean housing association
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    • v.11 no.1
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    • pp.103-113
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    • 2000
  • The main purpose of this paper is to provide useful infonnation for residential site design in Korea by analyzing Japanese case. The important characteristics of Japanese design techniques are follows. First, the characteristics of residential site development can be expressed as "eco-friendly" and "public order". The patterns of spatial use also focuses on living environment improvement. Second, variety replaces unity in internal mobility plans. For example the width of street, size of park are not same in a site boundary. Third, to avoid street parking and commodity exhibition, commercial buildings are set-back and public parking lot is constructed. Fourth, fences of private house are made of trees rather than bricks. It connects green environment between internal and external space of houses.nal space of houses.

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Assessment of Landfill Hazard Using the Value-Structured Approach (가치구조화기법에 의한 매립지 유해성 등급화)

  • Hong, Sang-Pyo;Kim, Jung-Wuk
    • Journal of Environmental Impact Assessment
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    • v.6 no.1
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    • pp.93-103
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    • 1997
  • LHR(Landfill Site Hazard Ranking Model) was developed for ranking the relative hazard of landfill sites by using the method of value-structured approach. LHR consists of combining a multiattribute decision-making method with a Qualitative risk assessment approach. A pairwise com parisian method was applied to determine weights of landfill site factors related. To determine the hazard of landfill site, hydrogeological factors, waste characteristics factors and receptors factors were evaluated by LHR. LHR can help decision-makers prioritization of remediation of landfill sites through the relatively convenient and concise evaluation method of landfill site features related. LHR focuses mainly on pathways of groundwater and surfacewater for evaluating landfill hazard to receptors including humans. To validiate the applicability of LHR, Nanjido Landfill site, Metropolitan Landfill site, and Hwasung Landfill site were evaluated.

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Development of Site Classification System and Modification of Site Coefficients in Korea Based on Mean Shear Wave Velocity of Soil and Depth to Bedrock (기반암 깊이와 토층 평균 전단파속도를 이용한 국내 지반분류 방법 및 지반 증폭계수 개선)

  • Kim, Dong-Soo;Lee, Sei-Hyun;Yoon, Jong-Ku
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.1C
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    • pp.63-74
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    • 2008
  • Site response analyses were performed based on equivalent linear technique using the local geologic and dynamic site characteristics, which include soil profiles, shear wave velocity profiles and depth to bedrock for 125 sites collected in Korean Peninsula. From the results of site response analyses, 2-parameters site classification system based on the combination of mean shear wave velocity of soil and depth to bedrock was newly recommended for regions of shallow bedrock depth in Korea. First, as the borders of bedrock depth (H) for site classification were determined as 10m and 20m, the soil sites were divided into 3 classes as $H_1$, $H_2$ and $H_3$ sites. And then, the 3 site classes were subdivided into 7 classes based on the mean shear wave velocity of soil ($V_{s,soil}$). The feasibility of new site classification system was verified and the representative site coefficients ($F_a$ and $F_v$) and design response spectrum were suggested by analyzing uniform trend and dispersion of site coefficients for each site class. The suggested site coefficients and the regression curves present the nonlinear characteristics of soils according to the change of rock outcrop acceleration with uniform trend effectively. From the comparison between the mean values of response spectrum which was acquired from the site response analysis and the suggested design response spectrum, there was a little difference in some of site classes and it was verified to adjust the integration interval to make it more suitable for the site condition in Korea.