• Title/Summary/Keyword: single-pass

Search Result 485, Processing Time 0.023 seconds

DNA Sequencing Analysis Technique by Using Solid-State Nanopore (고체상 나노구멍을 이용한 DNA 염기서열 분석기술)

  • Kim, Tae-Heon;Pak, James Jung-Ho
    • Journal of Sensor Science and Technology
    • /
    • v.21 no.5
    • /
    • pp.359-366
    • /
    • 2012
  • Nanopore DNA sequencing is an emerging and promising technique that can potentially realize the goal of a low-cost and high-throughput method for analyzing human genome. Especially, solid-state nanopores have relatively high mechanical stability, simple surface modification, and facile fabrication process without the need for labeling or amplification of PCR (polymerized chain reaction) in DNA sequencing. For these advantages of solid-sate nanopores, the use of solid-state nanopores has been extensively considered for developing a next generation DNA sequencing technology. Solid-state nanopore sequencing technique can determine and count charged molecules such as single-stranded DNA, double-stranded DNA, or RNA when they are driven to pass through a membrane nanopore between two electrolytes of cis-trans chambers with applied bias voltage by measuring the ionic current which varies due to the existence of the charged particles in the nanopore. Recently, many researchers have suggested that nanopore-based sensors can be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000.

Sea Tangle Supplementation Alters Intestinal Morphology in Streptozotocin-induced Diabetic Rats and Lowers Glucose Absorption

  • Lee, Kyeung-Soon;Seo, Jung-Sook;Choi, Young-Sun
    • Food Science and Biotechnology
    • /
    • v.16 no.6
    • /
    • pp.879-883
    • /
    • 2007
  • This study examined whether dietary supplementation with sea tangle alters the intestinal morphology of streptozotocin-induced diabetic rats and affects the glucose absorption rate. Forty male Sprague-Dawley rats were divided into 2 groups and fed either a control (AIN76-based) diet or a sea tangle-supplemented diet. After 3 weeks, 10 rats in each group received an intramuscular injection of streptozotocin (45 mg/kg BW), and feeding was continued for 3 additional weeks. Dietary supplementation with sea tangle resulted in a lower fasting plasma glucose level compared with the control diet in diabetic rats. Scanning electron micrographs revealed serious damage to the jejunal villi of diabetic rats fed the control diet, whereas supplementation with sea tangle alleviated the damage. In a separate experiment, 20 male Sprague-Dawley rats were divided into 2 groups and fed either a control diet or a sea tangle-supplemented diet for 5 weeks, and fasted rats were subjected to in situ single-pass perfusion. The glucose absorption rate determined in the absence of digesta was decreased by 34% in the jejunum of rats fed a sea tangle diet compared with those fed a control diet. In conclusion, sea tangle supplementation lowered glucose absorption rate, altered intestinal morphology, and appeared to protect villi from damage caused by diabetes mellitus.

A Security-Enhanced Identity-Based Batch Provable Data Possession Scheme for Big Data Storage

  • Zhao, Jining;Xu, Chunxiang;Chen, Kefei
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.12 no.9
    • /
    • pp.4576-4598
    • /
    • 2018
  • In big data age, flexible and affordable cloud storage service greatly enhances productivity for enterprises and individuals, but spontaneously has their outsourced data susceptible to integrity breaches. Provable Data Possession (PDP) as a critical technology, could enable data owners to efficiently verify cloud data integrity, without downloading entire copy. To address challenging integrity problem on multiple clouds for multiple owners, an identity-based batch PDP scheme was presented in ProvSec 2016, which attempted to eliminate public key certificate management issue and reduce computation overheads in a secure and batch method. In this paper, we firstly demonstrate this scheme is insecure so that any clouds who have outsourced data deleted or modified, could efficiently pass integrity verification, simply by utilizing two arbitrary block-tag pairs of one data owner. Specifically, malicious clouds are able to fabricate integrity proofs by 1) universally forging valid tags and 2) recovering data owners' private keys. Secondly, to enhance the security, we propose an improved scheme to withstand these attacks, and prove its security with CDH assumption under random oracle model. Finally, based on simulations and overheads analysis, our batch scheme demonstrates better efficiency compared to an identity based multi-cloud PDP with single owner effort.

Activated Carbon-Nickel (II) Oxide Electrodes for Capacitive Deionization Process

  • Gandionco, Karl Adrian;Kim, Jin Won;Ocon, Joey D.;Lee, Jaeyoung
    • Applied Chemistry for Engineering
    • /
    • v.31 no.5
    • /
    • pp.552-559
    • /
    • 2020
  • Activated carbon-nickel (II) oxide (AC-NiO) electrodes were studied as materials for the capacitive deionization (CDI) of aqueous sodium chloride solution. AC-NiO electrodes were fabricated through physical mixing and low-temperature heating of precursor materials. The amount of NiO in the electrodes was varied and its effect on the deionization performance was investigated using a single-pass mode CDI setup. The pure activated carbon electrode showed the highest specific surface area among the electrodes. However, the AC-NiO electrode with approximately 10 and 20% of NiO displayed better deionization performance. The addition of a dielectric material like NiO to the carbon material resulted in the enhancement of the electric field, which eventually led to an improved deionization performance. Among all as-prepared electrodes, the AC-NiO electrode with approximately 10% of NiO gave the highest salt adsorption capacity and charge efficiency, which are equal to 7.46 mg/g and 90.1%, respectively. This finding can be attributed to the optimum enhancement of the physical and chemical characteristics of the electrode brought by the addition of the appropriate amount of NiO.

Microflora and Color of Soybean Paste Sterilized by Counterflow Hat Exchanger (향류식 열교환기에 의하여 멸균된 된장의 미생물군 및 색도)

  • 유승곤;김인호;김종생;최성현;오만진;김용국;이인기
    • KSBB Journal
    • /
    • v.13 no.6
    • /
    • pp.724-729
    • /
    • 1998
  • To develop a large scale countercurrent single pass heat exchanger for continuous transportation and sterilization of soybean paste, microflora and color value of sterilized soybean paste were examined at various sterile condition. Aerobes, anaerobes, molds, yeasts and lactic acid bacteria were 5.1 x 107 CFU/g, 7.1 x 107 CFU/g, 2.6 x 105 CFU/g, 4.3 x 106 CFU/g, 1.3 x 107 CFU/g, respectively in raw soybean paste. In gold band ampoule test, aerobes and anaerobes of soybean paste were viable up to 90$^{\circ}C$, but become unviable at 100$^{\circ}C$. Molds decreased rapidly and yeasts decreased slowly from 70$^{\circ}C$. Lactic acid bacteria were unviable at 60$^{\circ}C$ within 10 min. In color test, Hunter L, a, and b values of soybean paste were 50.2, +5.6, and +17.8, respectively. After heating in polyethylene film bag at 80$^{\circ}C$, Hunter values were not so much changed and become 50.2, +4.7, and +19.7, respectively. The micorflora and color of soybean paste sterilized in a large scale heat exchanger system resulted in very similar to those of gold band ampoule and polyethylene film bag by effective heat transfer.

  • PDF

Effective Trapping of Suspended Solids by Biofilter of Intensive Bioproduction Korean System

  • Kim, In-Bae;Jo, Jae-Yoon
    • Journal of Aquaculture
    • /
    • v.21 no.3
    • /
    • pp.181-183
    • /
    • 2008
  • It has been a very hard problem to reduce solids especially suspended solids (SS) in recirculating aquaculture systems. Present description is based on the performance of trapping SS by the biofilter of Intensive Bio-production Korean (IBK) system which is originally developed for nitrification. We found out that this filter has an excellent capability to remove SS in addition to nitrification. Filter element used here is corrugated plastic roofing plates readily available in the market, and cheaper than specially developed and patented products. It is easy to maintain the system, and requires low power consumption to operate for the treatment of a large amount of water. With 2 pumps of 5 hp each, about 500 $m^3$ of water is treated per hour. Flow speed in the filter was 2.6 mm/sec on average. This low flow speed and very large amount of water treated are the reasons for very effective trapping of fine SS. Upon single pass through this filter, 74.5% of total SS and 40% of non-settleable SS were removed. Wherever this filter is employed in recirculating fish farms water keeps high clarity, this having also been empirically ascertained.

A Fundamental Study on the Receiver Front-End of Satellite Communication (MMIC를 위한 위성통신 수신 전단부의 기초 연구)

  • Chin, Youn-Kang;Yoon, Hyun-Bo;Kang, Hee-Chang;Park, Yhl;Cho, Gwang-Rae
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.13 no.4
    • /
    • pp.277-284
    • /
    • 1988
  • A 12GHz low-noise amplifier, a single gate GaAs MESFET mixer, and a low-pass filter have been fabricated for DBS receiver applications by using MIC technology. Each subsystem contains DC block with symmetric line and ship capacitor, respectively. The frequency converter with chip capacitor exhibits a 20-23 dB conversin gain with a RF bandwidth of 11.581-11.981 GHz and an IF bandwidth of 581-981MHz. Rf bandwidth of 12.1GHz and an IF banewidth of 1GHz.

  • PDF

Phase-change optical media for computer data storage (컴퓨터 정보저장용 상변화형 광기록매체)

  • 김명룡
    • Electrical & Electronic Materials
    • /
    • v.8 no.2
    • /
    • pp.229-236
    • /
    • 1995
  • Multimedia has created a system environment that needs a combination of diverse peripherals, faster I/O, and easier configuration. The sheer volume of data one can expect with multimedia hardware and applications storage systems of higher capacity and faster data transfer rate. Unlike the magneto-optical(MO)disk technology which uses bias magnetic field in writing, both the reading and the writing in the phase change (PC) technology are performed only by laser light. In PC optical media, an active layer is reversibly converted between amorphous state and crystalline state by changing irradiation conditions of focused laser beam. Thus, as compared with MO disk, the PC disk has such great advantages that signals can be reproduced by change of reflectance of laser beams in the same manner as the compact disc. The reflectivity of a phase-change spot can be altered in a single pass under the head only through modulation of laser power. The principles and the current status of phase-change optical recording media combined with possible applications are discussed in the present article.

  • PDF

Thermodynamic Analysis of Re-liquefaction Cycle of LNG Boil-off Gas (LNG 증발기체의 재액화 사이클에 대한 열역학 해석)

  • Chin, Young-Wook;Moon, Jeong-Woo;Lee, Yoon-Pyo;Chang, Ho-Myung
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.19 no.7
    • /
    • pp.485-490
    • /
    • 2007
  • The LNG BOG re-liquefaction system for LNG carriers was designed based on the Claude refrigeration cycle and the thermodynamic analysis was carried out in order to find the design point of the three heat exchangers constituting the system. The thermodynamic analysis revealed that the system state could be defined by the three cold endpoint temperatures of the three-pass heat exchanger. Hence the iso-lines of the specific liquefaction work, taken as the performance indicator, were presented in terms of those three temperatures and discussed. The system was found most economical when those three temperatures approached a single temperature of $-140^{\circ}C$ and thus this system state could be taken as the design point for the heat exchangers.

The Effects of Welding Process Parameters on Weld bead Width in GMAW Processes (GMAW 공정 중 용접 변수들이 용접 폭에 미치는 영향에 관한 연구)

  • 김일수;권욱현;박창언
    • Journal of Welding and Joining
    • /
    • v.14 no.4
    • /
    • pp.33-42
    • /
    • 1996
  • In recent years there has been a significant growth in the use of the automated and/or robotic welding system, carried out as a means of improving productivity and quality, reducing product costs and removing the operator from tedious and potentially hazardous environments. One of the major difficulties with the automated and/or robotic welding process is the inherent lack of mathematical models for determination of suitable welding process parameters. Partial-penetration, single-pass bead-on-plate welds were fabricated in 12mm AS 1204 mild steel flats employing five different welding process parameters. The experimental results were used to develop three empirical equations: curvilinear; polynomial; and linear equations. The results were also employed to find the best mathematical equation under weld bend width to assist in the process control algorithms for the Gas Metal Arc Welding(GMAW) process and to correlate welding process parameters with weld bead width of bead-on-plates deposited. With the help of a standard statistical package program. SAS, multipe regression analysis was undertaken for investigating and modeling the GMAW process, and significance test techniques were applied for the interpretation of the experimental data.

  • PDF