• The Plant Pathology Journal
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• 제37권3호
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• pp.291-298
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• 2021

False smut caused by Ustilaginoidea virens is an important rice fungal disease that significantly decreases its production. In the recent past, conventional methods have been developed for its detection that is time-consuming and need high-cost equipments. The research and development in nanotechnology have made it possible to assemble efficient recognition interfaces in biosensors. In this study, we present a simple, sensitive, and selective oxidized graphene-based geno-biosensor for the detection of rice false smut. The biosensor has been developed using a probe DNA as a biological recognition element on paper electrodes, and oxidized graphene to enhance the limit of detection and sensitivity of the sensor. Probe single-stranded DNA (ssDNA) and target ssDNA hybridization on the interface surface has been quantitatively measured with the electrochemical analysis tools namely, cyclic voltammetry, and linear sweep voltammetry. To confirm the selectivity of the device, probe hybridization with non-complementary ssDNA target has been studied. In our study, the developed sensor was able to detect up to 10 fM of target ssDNA. The paper electrodes were employed to produce an effective and cost-effective platform for the immobilization of the DNA and can be extended to design low-cost biosensors for the detection of the other plant pathogens.

• 산업식품공학
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• 제21권3호
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• pp.191-200
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• 2017

There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.

• Nuclear Engineering and Technology
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• 제54권10호
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• pp.3816-3823
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• 2022

This work analyzed the dosimetric difference between the intensity modulated radiotherapy (IMRT), partial/single/double-arc volumetric modulated arc therapy (PA/SA/DA-VMAT) techniques in treatment planning for treating more than one target of lung cancer at different isocenters. IMRT and VMAT plans at different isocenters were created systematically using a Harold heterogeneous lung phantom. The conformity index (CI), homogeneity index (HI), gradient index (GI), dose-volume histogram and mean and maximum dose of the PTV were calculated and analyzed. Furthermore, the dose-volume histogram and mean and maximum doses of the OARs such as right lung, contralateral lung and non GTV were determined from the plans. The IMRT plans showed the superior target dose coverage, higher mean and maximum values than other VMAT techniques. PA-VMAT technique shows more lung sparing and DA-VMAT increases the V_5/10/20 values of contralateral lung than other VMAT and IMRT techniques. The IMRT technique achieves highly conformal dose distribution to the target than other VMAT techniques. Comparing to the IMRT plans, the higher V_5/10/20 and mean lung dose were observed in the contralateral lung in the DA-VMAT.

• The Journal of Korean Physical Therapy
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• 제35권2호
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• pp.43-47
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• 2023

Purpose: This study compared the movement control ability of the ankle joint according to the type of muscle contraction, namely, eccentric or concentric contractions. Methods: Thirty-four healthy adult subjects participated in this study. As a single group, before the experiment, the subjects were trained on achieving the required position of the ankle around the target point by manually controlling the ankle dorsiflexion by 10°. Concentric contraction starts at 0° and continues until the target point of 10° is reached. During an eccentric contraction, the ankle joint starts at 20° ankle dorsiflexion and continues till the target point is reached. Movements using eccentric contraction and concentric contraction were randomly performed 3 times each. Results: The results of comparing the difference in the movement control ability of each type of muscle contraction of ankle dorsiflexion showed that the measurement-remeasurement error was significant in eccentric contraction. Conclusion: In this study, we found a difference in the ability to control movement according to whether the contraction is eccentric or concentric. Therefore, we propose that the ability to control movement is affected by the type of muscle contraction.

• 국제학술발표논문집
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• The 4th International Conference on Construction Engineering and Project Management Organized by the University of New South Wales
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• pp.262-268
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• 2011

According to the United Nations Framework Convention on Climate Change (UNFCCC), many countries around the world have been concerned with reducing Greenhouse Gas (GHG) emissions. Reducing the level of building energy consumption is particularly important in bringing GHG down. Because of this, many countries including the US and the EU are enforcing energy-related policies. However, these policies are focused on management of single types of buildings such as public buildings and office buildings, instead of management on a national level. Thus, although various policies have been enforced in many countries, CO₂ management on a national level is still not an area of focus. Therefore, this study proposed a community-based CO₂ management process that allows government-led GHG management. The minimum unit of the community in this study is a plot, and the process consists of three steps. First, the current condition of the GHG emission was identified by plot. Second, based on the identified results, the GHG emission reduction target was distributed per plot by reflecting the weighted value according to (i) the target CO₂ reduction in the buildings in the standard year, (ii) region, and (iii) building usage and size. Finally, to achieve the allocated target reduction, building energy management was executed according to the properties of the building located on each plot. It can be expected that the proposed community-based CO₂ management process will enable government-level GHG management, through which environment-friendly building construction can be promoted.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2000년도 춘계 학술발표회 논문집 Proceedings of EESK Conference-Spring
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• pp.367-374
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• 2000

This study investigates the effect of hysteretic characteristics to the Inelastic Demand Spectrum (IDS) which was expressed by an acceleration(Sa) and a displacement response spectrum (Sd). Elasto Perfectly Plastic(EPP) model is used in this study and inelastic demand spectrum (Sa vs, Sd) are obtained from a given target ductility ratio. For a given target ductility ratio IDS can be obtained by using nonlinear time history analysis of single degree of system with forth five recorded earthquake ground motions for stiff soil site. The effect EPP model under demand spectrum is investigated by ductility factor and natural frequency. According to the results obtained in this study IDS has dependency on ductility factor and natural frequency.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 하계학술대회 논문집 C
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• pp.2128-2130
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• 2004

High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on the electrodes simultaneously. This method is based on redox of an electrochemical ligand.

• ETRI Journal
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• 제32권6호
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• pp.921-931
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• 2010

It has been shown that the principal subspace-based multichannel Wiener filter (MWF) provides better performance than the conventional MWF for suppressing interference in the case of a single target source. It can efficiently estimate the target speech component in the principal subspace which estimates the acoustic transfer function up to a scaling factor. However, as the input signal-to-interference ratio (SIR) becomes lower, larger errors are incurred in the estimation of the acoustic transfer function by the principal subspace method, degrading the performance in interference suppression. In order to alleviate this problem, a principal subspace modification method was proposed in previous work. The principal subspace modification reduces the estimation error of the acoustic transfer function vector at low SIRs. In this work, a frequency-band dependent interpolation technique is further employed for the principal subspace modification. The speech recognition test is also conducted using the Sphinx-4 system and demonstrates the practical usefulness of the proposed method as a front processing for the speech recognizer in a distant-talking and interferer-present environment.

• BMB Reports
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• 제50권11호
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• pp.554-559
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• 2017

MicroRNAs (miRNAs) are ~22nt-long single-stranded RNA molecules that form a RNA-induced silencing complex with Argonaute (AGO) protein to post-transcriptionally downregulate their target messenger RNAs (mRNAs). To understand the regulatory mechanisms of miRNA, discovering the underlying functional rules for how miRNAs recognize and repress their target mRNAs is of utmost importance. To determine functional miRNA targeting rules, previous studies extensively utilized various methods including high-throughput biochemical assays and bioinformatics analyses. However, targeting rules reported in one study often fail to be reproduced in other studies and therefore the general rules for functional miRNA targeting remain elusive. In this review, we evaluate previously-reported miRNA targeting rules and discuss the biological impact of the functional miRNAs on gene-regulatory networks as well as the future direction of miRNA targeting research.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.17-17
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• 2005

In this presentation, we will discuss several recent achievements developed in my laboratory for microarray-based diagnosis of human genetic mutations including HNF-1 and BRCA1 mutations. To determine the presence of the genetic mutations in a human sample, we prepared allele-specific oligonucleotide chips from selected mutation sites and generated target probes using a tow-step method for Cy-3 DNA $samples^{1)}$ or in vitro transcription of promoter-tagged PCR products for Cy-3 RNA $samples^{2)}$. Hybridization of the target probes to the chips successfully identified all of the genotypes for the tested sites. For more reliable diagnosis, we also employed single base extension (SBE) reaction and zip-code microarray technique for our strategy. Particularly we developed an efficient PNA zip-code microarray for the detection of $HNF-1{\alpha}$ $mutations^{3)}$. Using multiplex SBE reactions and zip-code strategy, we were able to correctly diagnose several mutation sites in exon 2 of $HNF-1{\alpha}$ with a wild-type and mutant including a MODY3 patient. These works represent successful applications of DNA microarray technology for the diagnosis of human genetic mutations.