• Journal of Korean Institute of Industrial Engineers
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• v.37 no.4
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• pp.279-288
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• 2011

The past researches focused on the supply chain network that consists of factories, distribution centers and retailers for single product type. This research is required because the factory for single product type is advanced to reconfigurable type in order to produce various products, according to customers' various purchase forms and time. This research is also required because in the past researches, the material flows from factories to distribution centers and from distribution centers to retailers, but recently, there are material flows between distribution centers. The supply chain network in this research consists of reconfigurable manufacturing system, multi-layered distribution centers, and retailers. A simulator is developed to analyze the material flow on the supply chain network. The developed simulator is web-based designed by using Java Server Page and MS-SQL, so as to maximize the convenience for users.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2006.05a
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• pp.187-192
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• 2006

In the absence of a clear command and control structure, a key challenge in supply chain management is the coordination and alignment of the supply chain members who pursue divergent and often conflicting goals. The newsvendor model is typically used as a framework to quantify the cost of misalignment and to assess the impact of coordination initiatives. This paper considers a fuzzy approach for the newsvendor problem which includes a single manufacturer and a single retailer. We use several fuzzy parameters in the model such as the demand, the wholesale price, and the market sales price. We apply a coordination policy, referred to as buyback, to solve the fuzzy newsvendor problem. Based on the buyback policy, the optimal order quantity of the retailer can be computed, and the possible profits of the members in the supply chain can be calculated with minimum sharing of private information. Focusing on the fuzzy model with buyback policy for the newsvendor problem, we illustrate exemplary fuzzy models. We also illustrate an integration model, which extends a single-manufacturer-single-retailer model to the single-manufacturer-multiple-retailer setting. In the extended model, we consider three coordination policies including quantity discount, profit sharing, and buyback, as well as non-coordination case.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.2
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• pp.144-149
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• 2001

For the production and purification of a single chain human insulin precursor, four types of fusion peptides $\beta$-galactosidase (LacZ), maltose binding protein (MBP), glutathione-S-transferase (GST), and (His)(sub)6-tagged sequence (HTS) were investigated. Recombinant E. coli harboring hybrid genes was cultivated at 37$\^{C}$ for 1h, and gene induction occurred when 0.2mM of isopropyl-D-thiogalactoside (IPTG) was added to the culture broth, except for E. coli BL21 (DE3) pLysS harboring a pET-BA cultivation with 1.0mM IPTG, followed by a longer than 4h batch fermentation respectively. DEAE-Sphacel and Sephadex G-200 gel filtration chromatography, amylose affinity chromatography, glutathione-sepharose 4B affinity chromatography, and a nickel chelating affinity chromatography system as a kind of immobilized metal ion affinity chromatography (IMAC) were all employed for the purification of a single chain human insulin precursor. The recovery yields of the HTS-fused, GST-fused, MBP-fused, and LacZ-fused single chain human insulin precursors resulted in 47%, 20%, 20%, and 18% as the total protein amounts respectively. These results show that a higher recovery yield of the finally purified recombinant peptides was achieved when affinity column chromatography was employed and when the fused peptide had a smaller molecular weight. In addition the pET expression system gave the highest productivity of a fused insulin precursor due to a two-step regulation of the gene expression, and the HTS-fused system provided the highest recovery of a fused insulin precursor based on a simple and specific separation using the IMAC technique.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.12
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• pp.74-82
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• 2008

In this paper, we propose a P2P based IPTV system using a multiple chain architecture. Proposed system is robust to the peer churn. As opposed to the internet the IPTV network managed by a single ISP has fewer bandwidth constraints and end-to-end connectivity. So, we emphasize preferentially robustness of a P2P network in IPTV environment. A single chain structure which was proposed previously emphasizes simplicity, however there exists considerable delay time at the end part of peers as the number of peers increasing. As a solution to the problem, we propose the scheme which seperates the chain into several levels and again divides each level into spans to diminish a delay time. Though the chain is separated into level and span, basic structure of proposed scheme is still a chain structure. So the scheme simplifies the recovery procedure caused by join or departure of peers. We will show the improved performance of proposed scheme rather than single chain structure with respect to the delay time and reliability.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.330-342
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• 2018

The Streptavidin and Biotin system has been studied most extensively as the high affinity non-covalent binding of Biotin to STR ($K_D=10^{-14}M$) and four Biotin binding sites in tetrameric Streptavidin makes this system useful for the production of multivalent antibody. For the application of this system, we cloned Streptavidin amplified from Streptomyces avidinii chromosome by PCR and fused to gene of hAY4 single-chain Fv antibody specific to death receptor 4 (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand. The hAY4 single-chain Fv antibody fused to Streptavidin expressed in Escherichia coli showed 43 kDa monomer in heated SDS-PAGE. However, this fusion protein shown in both non-heated SDS-PAGE and Size-exclusion chromatography exhibited 172 kDa as a tetramer suggesting that natural tetramerization of Streptavidin by non-covalent association induced hAY4 single-chain Fv tetramerization. This fusion protein retained a Biotin binding activity similar to natural Streptavidin as shown in Ouchterlony assay and ELISA. Death receptor 4 antigen binding activity of purified hAY4 single-chain Fv fused to Streptavidin was also confirmed by ELISA and Westernblot. In addition, surface plasmon resonance analysis showed 60-fold higher antigen binding affinity of the hAY4-STR than monomeric hAY4 ScFv due to tetramerization. In summary, hAY4 single-chain Fv fused to Streptavidin fusion protein was successfully expressed and purified as a soluble tetramer in E. coli and showed both Biotin and DR4 antigen binding activity suggesting possible production of bifunctional and tetrameric ScFv antibody.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.48-52
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• 2015

Control of polymer conformations in heterogeneous confinement plays an important role in natural and engineering processes. We present a simulation study on the conformational structure and dynamics of a single, flexible polymer in a dense array of nanoposts with different sizes and separations, especially, when the volume of the interstitial space formed between four nanoposts is less than the size of the polymer chain. When a polymer is placed in the array of nanoposts, the size of polymer increases compared with that in the absence of nanoposts due to the confinement effect. It is shown that when a polymer is confined in the array of nanoposts the chain is elongated in the direction parallel to the nanoposts. As the interstitial volume between four nanoposts decreases either by increasing the nanopost diameter or by decreasing the separation between nanoposts, the chain elongation becomes more pronounced. On the contrary, the polymer size varies in a non-monotonic fashion, with an initial elongation followed by a chain contraction, as the interstitial volume is reduced both by increasing the nanopost diameter and decreasing the separation at the same time while keeping constant the width of the passageway between two nanoposts. The simulation analysis shows that the non-monotonic dependence of polymer size is determined by interplay between the chain alignment along the nanoposts in each interstitial volume and the chain spreading through passageways over several interstitial volume.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2007.11a
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• pp.104-107
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• 2007

In this paper, we propose the coordination scheme in a single-manufacturer-multiple-retailer supply chain system. Traditionally, the bargaining power of the single manufacturer is superior to those of retailers. The business environment supported by the e-business scheme may give rise to new business behavior. The multitude of retailer requests the manufacturer to relax the his unilateral lot-sizing policy while they are willing to compensate more than the manufacturer's expected loss caused by abandon his superior bargaining power. The side payment aggregated by the multiple retailers is transferred to the manufacturer according to the degree of the manufacturer's relaxation from the current unilateral lot-sizing policy.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.34 no.4
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• pp.25-34
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• 2011

In this paper, we investigate an inventory system where a single manufacturer purchases and processes raw materials in order to deliver finished goods to multiple retailers. Earlier study in this type of supply chain only consider a single raw material in order to produce finished goods, but we consider multi-raw materials in order to produce finished goods. Also, we develop an iterative solution procedure to find the order quantity for the finished goods and raw materials, and the number of shipments between manufacturer and retailers that minimizes the total cost per unit time of the raw materials ordering and holding, manufacture's setup and finished goods holding, the retailer's ordering and inventory holding. Numerical examples are presented to illustrate that jointly considering the total cost results in less total cost than that of considering them separately.

• Journal of KIISE:Information Networking
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• v.30 no.2
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• pp.207-221
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• 2003

A hash chain is highly efficient, attractive structure to use in electronic cash. Previous systems using hash chain were, however, either credit-based vendor-specific cash or debit-based general-purpose cash which lacks efficiency due to double spending problem. In this paper, we propose a new divisible cash system using hash chain. This newly proposed cash is general-purpose, debit-based, anonymous, and offline. The efficiency of the system results from its capacity to pay variable amounts with no additional costs. A client always performs a single blind signature in the withdrawal phase, independent of the length of the chain. During payment, a client performs a single challenge-and-response or generates a single signature, independent of the amount paid. This system provides a new refund mechanism, which uses a refund ticket, that allows clients to refund the unspent part of the chain without revealing any connection to the spent part.

• Management Science and Financial Engineering
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• v.22 no.1
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• pp.1-4
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• 2016

We consider a two-tier supply chain with multiplicative random yield. We focus on the supply chain performance with respect to the control scheme of determining the production lot size. The profit loss due to distributed control is analyzed to give an insight for devising efficient supply contracts.