• Biotechnology and Bioprocess Engineering:BBE
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• 제1권1호
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• pp.9-12
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• 1996

In order to produce the single chain precursor of a novel human insulin analogue, (B30-Homoserine) insulin, the fermentative behaviors of Escherichia coli JM103 were studied, which harbors pKBA plasmid carrying a hybrid gene in which the gene for a single chain precursor was fused with lacZ gene under tac promoter. The maximal induction of gene expression was achieved when more than 0.05 mM of isopropyl-$\beta$-D-thiogalactopyranoside(IPTG) was supplemented to fermentation medium after 4 h cultivation of E. coli, and followed by longer than 2-h fermentation. The hybrid protein of the single chain insulin precursor was isolated from cytoplasmic inclusion bodies by dissolving in 8M urea solution, and purified through DEAE-Sephacel and Sephadex G-200 column chromatographies with a recovery of 35%. The finally purified hybrid protein showed a single band on sodium dodecyl sulfate-polyacrylamide gel.

• 산업경영시스템학회지
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• 제37권4호
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• pp.116-125
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• 2014

In this paper, we investigate an inventory and production system in a three-layer supply chain system involving a single supplier, single manufacturer and multiple retailers. Earlier study in this type of supply chain only consider a single raw material in order to produce single item, but we consider raw materials in order to produce multiple items. It is assumed that the cycle time at each stage is an integer multiple of the adjacent downstream stage. We develop an iterative solution procedure to find the order quantity for the multiple items and raw materials that minimizes the supply chain-wide total cost per unit time of the supplier and manufacturer's raw materials ordering and holding, setup and finished items holding, the retailer's ordering and inventory holding. Numerical examples are presented to show that the proposed heuristic gives good performance.

• BMB Reports
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• 제30권3호
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• pp.177-181
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• 1997

We constructed a single-chain immunoglobulin in which the carboxyl end of the heavy chain variable domain is covalently joined to the amino terminus of the light chain variable domain via peptide linker and the carboxyl end of the light chain variable domain is linked to human ${\gamma}1$ Fc region through the hinge region. The molecule was expressed in Chinese hamster ovary cells, assembled into a dimeric molecule and secreted into the culture medium. The dimeric molecule (2E11) was purified from the culture supernatant by affinity chromatography on Protein G-Sepharose column. The size of the unreduced or reduced protein was the expected molecular weight of approximately 120 or 60 kDa, respectively, as assessed by SDS-polyacrylamide gel electrophoresis. The antigen-binding affinity of 2E11 was almost the same as that of a native antibody counterpart (CS131A), suggesting that the single-chain immunoglobulin may function like a native antibody.

• Management Science and Financial Engineering
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• 제12권1호
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• pp.1-34
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• 2006

In this paper, the impact of information sharing, possibly with some delay, on costs in a simple supply chain in which there are two participants, a single retailer and a single manufacturer, is considered. When participants in the supply chain do not use fully integrated EDI, some delay associated with information sharing is inevitable. A mathematical model that allows us to quantify the cost incurred by the manufacturer in the supply chain under information sharing, possibly with some delay, vs. no information sharing is presented. From this model, some managerial implications are gleaned.

• 미생물학회지
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• 제47권4호
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• pp.328-334
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• 2011

Leucine zipper motif는 여러 개의 주기적인 leucine 잔기로 구성되어 amphipathic alpha helix형태의 구조를 나타내며 소수성 결합에 의해 이량체를 형성한다. 이 leucine zipper motif를 single chain Fv 항체의 C-terminus에 도입하면 leucine zipper motif의 소수성 결합에 의해 amphipathic alpha helix의 이량체가 형성되면서 융합된 single chain Fv 항체의 이량체 (Dimer) 형성 또한 유도할 수 있다. 이량체 형태의 single chain Fv 항체는 2개의 항원 결합부위를 갖게 되므로 단량체 형태의(monomer) single chain Fv 항체에 비해 항원 결합력(Avidity)이 증가 될 것이다. 이 개념에 기초하여 이전 연구에서 제조된 단량체 형태인 닭 single chain Fv 항체인 8C3 ScFv 항체의 C-terminus에 leucine zipper motif를 도입하여 이량체 형태의 8C3 ScFv 항체를 개발하였다. 이량체 8C3 ScFv 항체는 가금류의 대표적인 기생충 질병인 coccidiosis를 유발하는 Eimerian sporozoite에 특이적으로 결합하는 기능을 나타내었다. 또한 이량체 8C3 ScFv 항체는 avidity 증가로 인하여 단량체에 비해 항원 결합력이 약 3배 증가됨을 확인할 수 있었으며 단백질 회수율 또한 2배 증가되는 부수적인 효과를 얻을 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.168-173
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• 2009

Human follicle-stimulating hormone (hFSH) is a pituitary glycoprotein that regulates follicular development and ovulation. Clinically, hFSH has been used to induce follicular growth in infertile women. The hormone is composed of heterodimers, including a common ${\alpha}$ subunit among the gonadotropin family and a hormone-specific ${\beta}$ subunit. Since assembly of the heterodimer is a rate-limiting step in the production of functional hFSH, transgenic clone cows carrying a single-chain hFSH transgene may efficiently produce functional hormone. Genes encoding the ${\alpha}$ and ${\beta}$ subunits of hFSH were linked using the C-terminal peptide sequence from the ${\beta}$ subunit of human chorionic gonadotropin. Bovine fetal fibroblasts were transfected with the gene construct, including the goat ${\beta}$-casein promoter and a single-chain hFSH coding sequence. Transfected fibroblasts were transferred into enucleated oocytes, and individual nuclear transfer (NT) embryos developed to the blastocyst stage were analyzed for the transgene by polymerase chain reaction. Seventy eight blastocysts (30.8%) were developed from 259 reconstructed embryos. Among these blastocysts, the hFSH gene was detected in 70.8% (34/48) of the embryos. Subsequent transfer of hFSH-transgenic clone embryos to 31 recipients results in 11 (35.5%) early pregnancies. However, all fetuses were lost before reaching day 180 of gestation. The results from this study demonstrated that bovine NT embryos carrying single-chain hFSH could be produced, and further extensive studies in which NT embryos are transferred to more recipients may give rise to single chain hFSH-transgenic cows for biomedical applications.

• 한국전자파학회논문지
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• 제24권10호
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• pp.994-1000
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• 2013

기존의 MIMO 시스템은 수신 안테나 수만큼 신호처리를 위한 RF 체인이 필요하다. 안테나를 늘릴 경우, RF 체인도 같이 늘어나기 때문에 전력 소비량이 급격하게 증가하게 된다. 이러한 이유로 더 많은 안테나의 MIMO 시스템을 전력이 제한적인 모바일 단말기에 적용하기에는 어려움이 있다. 본 논문에서는 이러한 문제를 해결하기 위하여 단일 RF 체인을 이용하여 다중 안테나의 신호를 수신하는 시스템을 제안한다. 제안하는 시스템은 STO(Sampling Timing Offset)을 동기 신호와 신호의 위상 각 추정을 통해 보상하였을 때 다중 RF chain을 사용하는 일반적인 MIMO 시스템과 유사한 성능을 낸다. 따라서 단일 RF chain을 통하여 전력 소비를 감소시키면서 다중 안테나의 MIMO-OFDM 시스템을 구현할 수 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.376-380
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• 1999

Constructs, encoding a single-chain variable fragment of a catalytic antibody 4f4f (scFv-4f4f) with glycosidase activity, were made by combining the coding sequences for the heavy and light chain variable domains with a sequence encoding a linker (GGGGS). Using three different plasmid systems, single-chain antibodies were expressed separately in Escherichia coli, demonstrating significant differences in the expression level and amounts in soluble form of the recombinant protein. The protein expression from pET3a-scFv-4f4f was up to 20% of the total soluble proteins and, more importantly, the proteins were mostly found in a soluble form. An SDS-PAGE analysis of the purified single-chain proteins, yielding higher than 5mg from a 1-1 culture, showed a single band corresponding to its molecular weight of 29,100. A preliminary study shows that the expressed scFv-4f4f is catalytically active. The catalytic parameters for the hydrolysis of p-nitrophenyl-$\beta$-D-glucopyranoside by scFv-4f4f are being investigated.

• 한국통신학회논문지
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• 제38A권10호
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• pp.885-892
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• 2013

최근 기존의 배열 안테나를 사용하는 MIMO(multi-input multi-output) 시스템의 단점을 극복하기 위하여 1개의 능동 소자와 주변의 기생 소자를 이용하는 ESPAR(electronically steerable parasitic array radiator) 안테나에 대한 연구가 이루어지고 있다. 이 안테나의 가장 큰 장점은 단지 1개의 RF(radio frequency) chain만을 사용하는 것이다. 단일 RF chain을 사용하기 때문에 하드웨어 복잡도가 높지 않다. ESPAR 안테나를 사용하는 빔 공간 MIMO 시스템의 경우 각각의 직교 기저 패턴에 심볼을 맵핑하여 송신한다. 본 논문에서는 저 복잡도, 저 전력의 MIMO 시스템을 위해 단일 RF chain을 사용하는 ESPAR 안테나를 이용하여 시스템을 구성하고 각각의 위상 편이 변조에 따른 성능을 분석한다. 빔 공간 MIMO 시스템은 기존의 MIMO 시스템과 유사한 성능을 낸다. BPSK(binary phase shift keying), QPSK(quadrature phase shift keying), 8PSK, 16PSK, 32PSK의 고차 변조에 대한 시스템 성능을 분석한 결과, 빔 공간 MIMO 시스템이 저 복잡도와 저 전력소비로 기존 신호 도메인의 MIMO 시스템과 유사한 성능 특성을 가지는 것을 확인하였다.

• 전기학회논문지
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• 제56권5호
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• pp.980-986
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• 2007

Boundary scan test structure(JTAG IEEE 1149.1 standard) that supports an internal scan chain is generally being used to test CUT(circuit under test). Since the internal scan chain can only have a single scan-in port and a single scan-out port; however, existing boundary test methods can not be used when multiple scan chains are present in CUT. Those chains must be stitched to form a single scan chain as shown in this paper. We propose an efficient boundary scan test structure that adds a circuit called Clock Group Register(CGR) for multiple clocks testing within the design of multiple scan chains. The proposed CGR has the function of grouping clocks. By adding CGR to a previously existing boundary scan design, the design is modified. This revised scan design overcomes the limitation of supporting a single scan-in port and out port, and it bolsters multiple scan-in ports and out ports. Through our experiments, the effectiveness of CGR is proved. With this, it is possible to test more complicated designs that have high density with a little effort. Furthermore, it will also benefit in designing those complicated circuits.