• Title/Summary/Keyword: simultaneous administration

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A Study on Antitoxic Effects of Pine Leaf Extracts against Cadmium Toxicity in Rats (송엽 추출물을 이용한 카드뮴 독성의 해독에 관한 연구)

  • 이종섭;박경옥
    • Journal of Environmental Health Sciences
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    • v.22 no.3
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    • pp.88-97
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    • 1996
  • This study was conducted to investigate the antitoxic effects of Pine Leaf extracts against cadmium toxicity. The experimental rats were divided into 5 groups, such as control group cadmium alone treatment group and simultaneous treatment groups of cadmium and three doses of Pine Leaf extracts. Each group was administered with different dose of Pine Leaf extracts such as 0.5 mg, 2.5 mg, 5.0 mg/kg wet weight in pallets for 12 weeks. Cadmium Chloride($CdCl_2$) was administered by 4 mg/kg body weight. The results were summarized as follows: The simultaneous administration of cadmium and Pine Leaf significantly more decreased cadmium concentration in liver tissues compared to the administration of cadmium only. When blood were measured, no significantly difference in haemoglobin, haematocrit, erythrocyte values compared to the administration of cadmium only, but RBC significantly more increased. The simultaneous administration of cadmium and Pine Leaf more no sigmificantly difference metallothionein concentration in liver than the administration of cadmium only. There were showed the histopathological slight changes in the liver and kidney tissues of rats.

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A Study on the Effects of Radix Menispermi Extracts Against Cadmium Chloride Sub-chronic Toxicity in Rats (카드뮴 중독에 대한 산두근 추출액의 해독효과에 관한 연구)

  • 이종섭;박경옥;이정미
    • Toxicological Research
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    • v.12 no.1
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    • pp.59-68
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    • 1996
  • This study was conducted to investigate the antitoxic effects of Radix Menispermi extracts against cadmium toxicity. The experimental rats were divided into 5 groups such as control group, cadmium alone treatment group and simultaneous treatment groups of cadmium and three doses of Radix Menispermi extracts. Each group was administered with different dose of Radix Menispermi extracts such as 0.55 mg, 1.10 mg, 1.65 mg/kg wet weight in pallets for 12 weeks. Cadmium Chloride $(CdCl_2)$ was administered by 4 mg/kg body weight. The results were summarized as follows: 1. The simultaneous administration of cadmium and Radix Menispermi significantly more decreased cadmium concentration in liver tissues compared to the administration of cadmium only (P < 0.05). 2. When blood were measured, no significantly difference in haemoglobin, haematocrit, erythrocyte values compared to the administration of cadmium only. 3. The simultaneous administration of cadmium and Radix Menispermi more increased metallothionein concentration in liver than the administration of cadmium only (P < 0.05). 4. There were the histopathological slight changes in the liver and kidney tissues of rats.

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Development of Single-tube Multiplex Immunocapture RT-PCR Assay for Simultaneous Detection of Two Pepper Tobamoviruses

  • Kim, Jae-Hyun;Choi, Gug-Seoun;Kim, Jeong-Soo;Lee, Sin-Ho;Choi, Jang-Kyung;Ryu, Ki-Ryun
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.164-167
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    • 2006
  • An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.

Development of a Multiplex Polymerase Chain Reaction Method for Simultaneous Detection of Genetically Modified Soy and Maize

  • Park, Kyoung-Sik;Kim, Mi-Gyeong;Leem, Dong-Gil;Yoon, Tae-Hyung;No, Ki-Mi;Hong, Jin;Kwon, Eun-Mi;Moon, Ae-Rie;Jeong, Ja-Young
    • Journal of Food Hygiene and Safety
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    • v.25 no.3
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    • pp.278-280
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    • 2010
  • This study was aimed to develop a novel qualitative multiplex polymerase chain reaction (PCR) for simultaneous detection of genetically modified (GM) soy and maize within a single reaction. The specific primers designed to detect four respective GM events (A2704-12, MON88017, Bt11, and MON863) were included in the tetraplex PCR system. Each of PCR products for four GM events could be distinguished by agarose gel based on their different lengths. The specificity and reproducibility of this multiplex PCR were evaluated. This multiplex PCR consistently amplified only a fragment corresponding to a specific inserted gene in each of the four GM events and also amplified all four of the PCR products in the simulated GM mixture. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM soy and maize in a single reaction.

Exploring Simultaneous Presentation in Online Restaurant Reviews: An Analysis of Textual and Visual Content

  • Lin Li;Gang Ren;Taeho Hong;Sung-Byung Yang
    • Asia pacific journal of information systems
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    • v.29 no.2
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    • pp.181-202
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    • 2019
  • The purpose of this study is to explore the effect of different types of simultaneous presentation (i.e., reviewer information, textual and visual content, and similarity between textual-visual contents) on review usefulness and review enjoyment in online restaurant reviews (ORRs), as they are interrelated yet have rarely been examined together in previous research. By using Latent Dirichlet Allocation (LDA) topic modeling and state-of-the-art machine learning (ML) methodologies, we found that review readability in textual content and salient objects in images in visual content have a significant impact on both review usefulness and review enjoyment. Moreover, similarity between textual-visual contents was found to be a major factor in determining review usefulness but not review enjoyment. As for reviewer information, reputation, expertise, and location of residence, these were found to be significantly related to review enjoyment. This study contributes to the body of knowledge on ORRs and provides valuable implications for general users and managers in the hospitality and tourism industries.

Simultaneous Detection of 10 Foodborne Pathogens using Capillary Electrophoresis-Based Single Strand Conformation Polymorphism

  • Oh, Mi-Hwa;Hwang, Hee-Sung;Chung, Bo-Ram;Paik, Hyun-Dong;Han, Sang-Ha;Kang, Sun-Moon;Ham, Jun-Sang;Kim, Hyoun-Wook;Seol, Kuk-Hwan;Jang, Ae-Ra;Jung, Gyoo-Yeol
    • Food Science of Animal Resources
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    • v.32 no.2
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    • pp.241-246
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    • 2012
  • This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.

Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

  • Kang, In Jeong;Kang, Mi-Hyung;Noh, Tae-Hwan;Shim, Hyeong Kwon;Shin, Dong Bum;Heu, Suggi
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.575-579
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    • 2016
  • Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the $63^{\circ}C$ as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

Multiple Plasma Peaks of Acetaminophen and Ranitidine after Simultaneous Oral Administration to Rats

  • Shim, Chang-Koo;Suh, Mi-Kyung
    • Archives of Pharmacal Research
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    • v.15 no.3
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    • pp.246-250
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    • 1992
  • Acetaminophen (AAP) and ranitidine (RT) were coadministered orally to nine rats, and the possible contribution of the gastric emptying to the plasma concentration profiles of them was examined. The drugs showed multiple plasma peaks similar to the respective ones after separated administration of each durg. IT Implies that there is no significant interaction between AAP and RT in terms of the gastric emptying or drug absorption. There were no significant linear correlations of the peak patterns (peak height and peak time) between AAP andd RT. It is contrary to the expectation from the biphasic gastric emptying (BGE) theory previously suggested for AAP and RT. The BGE theory. Therefore, seemed to have some draw-backs in explaining satisfactorily the multiple plasma peaks of AAP and RT. Two more doubts raised previously against the BGE theory were also discussed.

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