• Journal of Pharmaceutical Investigation
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• v.21 no.1
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• pp.33-41
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• 1991

A high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of theophylline(TP) and its metabolites, 1-methyluric acid (1-MU) and 1,3-dimethyluric acid (1,3-DMU), in rat plasma and urine. An $100\;{\mu}l$ aliquot of a plasma or urine sample was mixed with $250\;{\mu}l$ of acetonitrite and vortexed. After centrifugation, $200\;{\mu}l$ (plasma) or $20\;{\mu}l$ (urine) aliquot of the supernatant was dried by $N_2$ stream and redissolved in $100\;{\mu}l$ (plasma) or $200\;{\mu}l$ (urine) of the mobile phase. A $20\;{\mu}l$ of the mobile phase solution was injected onto a $C_{18}$ reversed-phase column. The column was maintained at $45^{\circ}C$ by the aid of electric heating jacket. The mobile phase was a 3%(v/v) methanol solution in deionized water which contains sodium acetate (100 mM) and tetrabutyl ammonium hydroxide (4 mM). pH of the mobile phase was adjusted 4.5 by the addition of acetic acid. Detection limits for TP, 1-MU, and 1,3-DMU in plasma were 0.2, 0.1 and $0.1\;{\mu}/ml$, respectively and the corresponding values in urine were all $5\;{\mu}g/ml$. Inter- and intra-day variability of the assay for all compounds in the plasma samples was less than 5.5 and 3.8%, respectively. The retention times for 1-MU, 1,3-DMU, and TP were approximately 7, 8.5 and 18 min, respectively. Sample preparation procedure used in this method was simple, rapid and reproducible. Renal clearance of TP and its metabolites in rats showed plasma concentration dependency indicating renal tubular secretion and reabsorption of them.

• Analytical Science and Technology
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• v.7 no.2
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• pp.245-251
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• 1994

In order to determine sodium chondroitin sulfate in the mixture, chondroitinase ABC was used for enzymatic reaction. The procedure was rapid, simple, quantitative and the HPLC analysis of ${\Delta}Di-6S$(2-actamido-2-deoxy-3-0-(${\beta}$-D-gluco-4-enepyranosyluronic acid)-6-0-sulfo-D-galactose) in the sodium chondroitin sulfate was obtained. The absorbance was measured at 230nm and detection limit was $1{\mu}g/ml$. When we applied this method to the drugs(capsule, opthalmic solution), it gave the mean contents of $100.01{\pm}1.58%$ and $99.89{\pm}1.80%$ respectively.

• Journal of Sensor Science and Technology
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• v.7 no.6
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• pp.418-425
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• 1998

Stereo cameras are the most widely used sensing systems for automated machines including robots to interact with their three-dimensional(3D) working environments. The position of a target point in the 3D world coordinates can be measured by the use of stereo cameras and the camera calibration is an important preliminary step for the task. Existing camera calibration techniques can be classified into two large categories - linear and nonlinear techniques. While linear techniques are simple but somewhat inaccurate, the nonlinear ones require a modeling process to compensate for the lens distortion and a rather complicated procedure to solve the nonlinear equations. In this paper, a method employing a neural network for the calibration problem is described for tackling the problems arisen when existing techniques are applied and the results are reported. Particularly, it is shown experimentally that by utilizing the function approximation capability of multi-layer neural networks trained by the back-propagation(BP) algorithm to learn the error pattern of a linear technique, the measurement accuracy can be simply and efficiently increased.

• Science of Emotion and Sensibility
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• v.14 no.2
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• pp.191-196
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• 2011

Fabric extraction force measured through nozzle tester reflects a comprehensive fabric handle. Nozzle tester takes advantage of low cost, and simple and fast operating procedure compared with KES system. The paper is to develop the semi-emprical equation for assessment of the fabric handle measured with nozzle tester on the basis of friction law. The variables considered in the equation arc fabric's frictional coefficient and drape coefficient which is determined in terms of fabric bending length and shear strain. The experiment of 12 different cotton and polyester fabrics and comparisons between experimental and theoretical results were conducted. Fabrics of high frictional coefficients, high bending length, and low shear strain showed high fabric handle forces (low handle values). The handle forces predicted from the equation agreed well with those measured, which indicates that the equation can be used to objectively evaluate fabric handle with respect to fabric's own properties and also provide an information for fabric design to improve the handle performance.

• Journal of Embryo Transfer
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• v.26 no.2
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• pp.141-145
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• 2011

Seven male Himalayan tahrs were strongly assumed to have accidently fed on foreign bodies in 2007. At the time, zoo caretakers witnessed missing fence padding, such as carpet, plastic awning, and ropes. The incident occurred the morning after the items had been set up to protect indigenous, wild long-tailed gorals from self injury caused by head butting the steel fence. Adult male Himalayan tahrs were obviously suspected of mostly eating the paddings mainly composed of carpet, thin and long plastic awning, and ropes. Even though they had not shown digestive problems, surgery was determined necessary in order to remove any indigestible foreign bodies. Left flank rumenotomy was conducted on seven male Himalayan tahrs from April to May, 2011. After anesthesia with xylazine, rumenotomy was performed on a concrete floor, with legs and head secured by ropes. No access to water and hay prior to operation for two days was needed to make the surgical procedure done quickly. Two sheets of small hand towels protecting against inflow of ruminal contents were beneficial during surgery. Antibiotics were administered intramuscularly for seven days. No abscesses at the surgical site were found after surgery. Like domestic ruminants, wild ruminants also ingest metalic or non-metalic, indigestible foreign bodies by accident. Therefore, simplified rumenotomy must be developed to apply to those wild animals to lengthen their longevity and to advance the quality of life in captivity. This case report is the first showing how to perform rumenotomy of Himalayan tahr, a wild ruminant, in Korea.

• Korean Journal of Clinical Laboratory Science
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• v.42 no.2
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• pp.63-70
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• 2010

Antibody screening and identification tests before blood transfusion are important because unexpected red antibodies can cause acute or delayed hemolytic transfusion reactions. Although a tube method was used for detecting unexpected antibodies, a column agglutination method has recently been used because of its simple procedure and a high detection of warm antibodies. This study investigated the frequency and distribution of unexpected antibodies in transfusion candidates during the recent 5 years, and transfusion characteristics in the identified cases. From January 2005 to December 2009, 46,923 sera of the cases from E hospital were screened and 98 sera were identified by the DiaMed-ID System. 272 cases (0.58%) showed positive results out of all 46,923 cases that underwent unexpected antibodies screening. Among them, unexpected antibodies were identified in 98 cases. The anti-Rh antibodies included in warm antibodies were the most frequently detected in 47 cases (47.96%). Anti-Lewis and anti-MNSs antibodies were detected in 11 cases (11.22%) and 6 cases (6.12%), respectively. Unidentified antibodies were detected in 6 cases (6.12%). Among the patients with unexpected antibodies, 43 cases (43.88%) had a history of previous transfusion. Anti-E was the most frequently detected antibody (4/14 cases, 30.77%) in the cases who had a previous history of transfusion and showed different screening results from negative to positive, This study may provide the basic data for the frequency and characteristics of red cell antibodies.

• Structural Engineering and Mechanics
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• v.56 no.4
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• pp.569-588
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• 2015

A comprehensive methodology is proposed for design of metallic dampers in seismic retrofit of earthquake-damaged frame structures. It is assumed that the metallic dampers remain elastic and only provide stiffness during frequent earthquake (i.e., earthquake with a 63% probability of exceedance in 50-year service period), while in precautionary earthquake (i.e., earthquake with a 10% probability of exceedance in 50-year service period), the metallic dampers yield before the main frame and dissipate most of the seismic energy to either prevent or minimize structural damages. Therefore by converting multi-story frame to an equivalent single-degree-of-freedom system, the added stiffness provided by metallic dampers is designed to control elastic story drifts within code-based demand under frequent earthquake, and the added damping with the combination of added stiffness influences is obtained to control structural stress within performance-based target under precautionary earthquake. With the equivalent added damping ratio, the expected damping forces provided by metallic dampers can be calculated to carry out the configuration and design of metallic dampers along with supporting braces. Based on a detailed example for retrofit of an earthquake-damaged reinforced concrete frame by using metallic dampers, the proposed design procedure is demonstrated to be simple and practical, which can not only meet current China's design codes but also be used in retrofit design of earthquake-damaged frame with metallic damper for reaching desirable performance objective.

• Computers and Concrete
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• v.26 no.2
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• pp.185-201
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• 2020

This research is devoted to investigate the bending and free vibration behaviour of laminated composite/sandwich plates and shells, by applying an analytical model based on a generalized and simple refined higher-order shear deformation theory (RHSDT) with four independent unknown variables. The kinematics of the proposed theoretical model is defined by an undetermined integral component and uses the hyperbolic shape function to include the effects of the transverse shear stresses through the plate/shell thickness; hence a shear correction factor is not required. The governing differential equations and associated boundary conditions are derived by employing the principle of virtual work and solved via Navier-type analytical procedure. To verify the validity and applicability of the present refined theory, some numerical results related to displacements, stresses and fundamental frequencies of simply supported laminated composite/sandwich plates and shells are presented and compared with those obtained by other shear deformation models considered in this paper. From the analysis, it can be concluded that the kinematics based on the undetermined integral component is very efficient, and its use leads to reach higher accuracy than conventional models in the study of laminated plates and shells.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1219-1227
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• 2014

A whole genome association (WGA) study was carried out to find quantitative trait loci (QTL) for sensory evaluation traits in Hanwoo. Carcass samples of 250 Hanwoo steers were collected from National Agricultural Cooperative Livestock Research Institute, Ansung, Gyeonggi province, Korea, between 2011 and 2012 and genotyped with the Affymetrix Bovine Axiom Array 640K single nucleotide polymorphism (SNP) chip. Among the SNPs in the chip, a total of 322,160 SNPs were chosen after quality control tests. After adjusting for the effects of age, slaughter-year-season, and polygenic effects using genome relationship matrix, the corrected phenotypes for the sensory evaluation measurements were regressed on each SNP using a simple linear regression additive based model. A total of 1,631 SNPs were detected for color, aroma, tenderness, juiciness and palatability at 0.1% comparison-wise level. Among the significant SNPs, the best set of 52 SNP markers were chosen using a forward regression procedure at 0.05 level, among which the sets of 8, 14, 11, 10, and 9 SNPs were determined for the respectively sensory evaluation traits. The sets of significant SNPs explained 18% to 31% of phenotypic variance. Three SNPs were pleiotropic, i.e. AX-26703353 and AX-26742891 that were located at 101 and 110 Mb of BTA6, respectively, influencing tenderness, juiciness and palatability, while AX-18624743 at 3 Mb of BTA10 affected tenderness and palatability. Our results suggest that some QTL for sensory measures are segregating in a Hanwoo steer population. Additional WGA studies on fatty acid and nutritional components as well as the sensory panels are in process to characterize genetic architecture of meat quality and palatability in Hanwoo.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.9
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• pp.88-93
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• 2003

This Paper described the simulation program development for helicopter. In the design of flight control system to accomplish some special missions like UAV, it is important to minimize the execution time obtaining a linear model from nonlinear model that is used for design of controller. The first step for this kind of purpose is to complete a nonlinear model that contains full dynamic characteristics. The second step is to get the trim values that are obtained from the nonlinear model by solving an algebraic equation. And then stability and control derivatives are derived through hovering to forward flight by numerical perturbation that will be used for linear model for a specified flight condition. The software program(HeliSim) is developed by using MATLAB GUI and will provide easy modeling procedure. The suggested method in this paper is much more simpler than any other method like a fully scale helicopter model. The advantage of our suggested method will reduce the computational time due to simple formula to extract a linear model from nonlinear model that will be beneficially used for flight control system of unmanned helicopter by some reduction of computational load.