• 제목/요약/키워드: simple procedure

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조직배양(組織培養)된 마우스복강거식세포에서의 인나균증식실험(人癩菌增殖實驗) - 1. 나결절(癩結節)에서 trypsin 처리(處理)에 의(依)한 인나균(人癩菌)의 정제(精製) (Growth Experiment of Mycobacterium Leprae in Cultured Mouse Peritoneal Macrophages - 1. Purification of viable Myco. leprae from biopsied lepromatous nodules by trypsinization method)

  • 양용태;유준;조세훈;김준걸
    • 대한미생물학회지
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    • 제7권1호
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    • pp.21-27
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    • 1972
  • 생검(生檢)한 나결절(癩結節)에서 간단(簡單)하고 효과적(效果的)인 방법(方法)인 trypsin 처리(處理)와 고속도유침(高速度遠沈)에 의(依)하여 동물접종목적(動物接種目的)에 사용(使用)할수 있는 인나균(人癩菌)의 정제(精製)에 대(對)하여 기술(記述)하였다. 본(本) 방법(方法)의 한 특징(特徵)은 그 정제과정중(精製過程中)에 재래식(在來式)인 나결절조직(癩結節組織)의 마쇄(磨碎)나 유액화조작(乳液化操作)을 전혀 포함(包含)시키지 않은 점(點)이다. 이 방법(方法)에 의(依)하여 정제(精製)된 인나균(人癩菌)을 한국산(韓國産) 다람쥐(Tamias sibiricus asiaticus, Gmelin)의 족저부(足底部) 및 이타조직내(耳朶組織內)에 접종(接種)하였던 바 균접종(菌接種) 8 및 12개월(個月)에 이르러 균증식양상(菌增殖樣狀)이 야기(惹起)됨이 관찰(觀察)되었다.

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근적외선분광광도법을 이용한 식용유지의 산가, 요오드가, 지방산 정량법에 관한 연구 (Quantitative Analysis of Acid Value, Iodine Value and Fatty Acids Content in Vegetable Oils by NIRS)

  • 김재관;최옥경;황선일;정진아;김윤성;박신희;손미희;권혜정;이정복;김종찬
    • 환경위생공학
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    • 제22권4호
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    • pp.65-76
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    • 2007
  • The possibility of rapid quantitative analysis of AV(Acid Value), IV(Iodine Value) and fatty acids in vegetable oils with NIRS(Near-infrared spectroscopy) was evaluated. A calibration equation calculated by MPLS regression technique was developed and correlation coefficient of determination for AV, IV, $C_{16:0},\;C_{18:0},\;C_{18:1},\;C_{18:2},\;C_{18:3},\;and\;C_{20:0}$ content were 0.9727, 0.997, 0.9805, 0.942, 0.9987, 0.9994, 0.9966, and 0.975 respectively. According to the data obtained from validation study, $R^2$ of contents of perilla, corn, soybean, rapaseed oils were 0.897, 0.993, 0.935, 0.707, 0.994, 0.996, 0.984, 0.798, SEP of contents of 0.185, 1.367, 0.899, 0.640, 1.498, 1.360, 0.476, 0.076 by MPLS. The results indicate that the NIRS procedure can potentially be used as a non-destructive analysis method for the rapid and simple measurement of AV, IV and fatty acids in vegetable oils.

21세기 다차원 커뮤니케이션과 매체예술의 문제 (The Problem of multi-dimension communication and 21st century Media Art)

  • 박기웅
    • 조형예술학연구
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    • 제3권
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    • pp.5-32
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    • 2001
  • At the beginning of 21st Century, human desire of communication is forwarding to the Cosmos The way of Communication is going to be not by simple methodology but by the complicated methodology with high technology. It will not be monologue communication but be interactive communication, that will make intelligence Infra, which could be able to communicate from the location of information is present to which is not present; and the multi-media which will be able to solve the technical problems of these communication; will be developed continually. In the genre of plastic art, there are no exception in these changedness. More developed and proceed to develope intermedia could be able of intercommunication. The more development of technology could be able to the more development of new plastic art. Furthermore the development of science make the genre of art to be changed. There are no exception of this changedness in any part of society, The art always has been guided by the person who has proceeding idea for new value. The 21st century plastic art will be in the procedure of the intercommunication. Human-being's concern is to communicate with Universe, and that will be multi-dimension$(4{\cdot}5{\cdot}6{\cdot}7{\cdots}dimension)$ communication beyond our usual recognition. To conjoin this, the possibility of cyber space expressing is going to be considered, and the way is being done by the development of the Media Art, which is able to go and back to the cyber-space. And the message will be so complicate beyond our recognition. Because we will need to communicate with various newly-built vocabulary, so we need to magnify the repertories of new vocabulary.

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Using ranked auxiliary covariate as a more efficient sampling design for ANCOVA model: analysis of a psychological intervention to buttress resilience

  • Jabrah, Rajai;Samawi, Hani M.;Vogel, Robert;Rochani, Haresh D.;Linder, Daniel F.;Klibert, Jeff
    • Communications for Statistical Applications and Methods
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    • 제24권3호
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    • pp.241-254
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    • 2017
  • Drawing a sample can be costly or time consuming in some studies. However, it may be possible to rank the sampling units according to some baseline auxiliary covariates, which are easily obtainable, and/or cost efficient. Ranked set sampling (RSS) is a method to achieve this goal. In this paper, we propose a modified approach of the RSS method to allocate units into an experimental study that compares L groups. Computer simulation estimates the empirical nominal values and the empirical power values for the test procedure of comparing L different groups using modified RSS based on the regression approach in analysis of covariance (ANCOVA) models. A comparison to simple random sampling (SRS) is made to demonstrate efficiency. The results indicate that the required sample sizes for a given precision are smaller under RSS than under SRS. The modified RSS protocol was applied to an experimental study. The experimental study was designed to obtain a better understanding of the pathways by which positive experiences (i.e., goal completion) contribute to higher levels of happiness, well-being, and life satisfaction. The use of the RSS method resulted in a cost reduction associated with smaller sample size without losing the precision of the analysis.

Detection of Salmonella in Milk by Polymerase Chain Reaction

  • Park, Weon-Sang
    • 한국식품위생안전성학회지
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    • 제15권3호
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    • pp.262-266
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    • 2000
  • 우유에 포함된 Salmonella enteritidis를 효과적으로 분리하는 방법을 찾고 이를 이용하여 우유속의 S. enteritidis의 량을 추정하는 방법을 개발하였다. 일정량의 S. enteritidis를 접종한 우유로부터 guanidine thiocyanate/phenol/chloroform을 이용하여 DNA를 추출한 후 중합효소반응으로 S. enteritidis 섬모항원 유전자를 선택적으로 검출함으로써 우유 1ml당 200 colony forming unit까지 검출이 가능하였고 전체 과정의 수행에 단지 5시간 정도 걸렸다. S. enteritidis 섬모항원 유전자를 cloning한 pGem-4-Sef B(-) DNA와 인위적으로 접종된 우유로부터 추출한 Salmonella DNA를 함께 중합효소반응으로 증폭한 후 제한효소로 잘라 전기영동을 행하여 band의 강도를 비교함으로써 Salmonella DNA copy수를 추정하는 것이 가능하였다.

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비파괴시험에 의한 콘크리트 항만시설물의 주요 물성치 평가 (Evaluation of Material Properties of Concrete Harbour Facilities Using Nondestructive Testing Methods)

  • 이진학;한상훈;박우선
    • 한국해안·해양공학회논문집
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    • 제23권1호
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    • pp.1-10
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    • 2011
  • 항만 리모델링을 위한 콘크리트 시설물에 대한 평가가 필요하며, 특히 리모델링이 결정된 경우에는 소파 블럭이나 케이슨 블록 등에 대한 재활용 여부를 평가하여 재활용이 가능할 경우에는 유용하는 것이 경제적인 리모델링에 도움이 될 수 있다. 이 연구에서는 콘크리트 항만시설물에 대한 건전성 평가 및 재활용성 평가를 위하여 기존의 초음파 속도법을 비롯하여 슈미트 해머를 이용한 반발경도법, 충격반향법, 표면파 속도법 등의 비파괴시험을 실시하였다. 국내 5개 항만에 대하여 비파괴시험 및 시편을 채취하였으며, 채취한 시편에 대한 압축강도 시험을 수행하여 비파괴시험 결과와 비교하였다. 비파괴시험결과와 압축강도 시험을 통하여 콘크리트 재료의 탄성계수와 압축강도 등을 평가하였다.

혈우병 B 환아의 전신마취 하 치과치료 : 증례보고 (DENTAL MANAGEMENT OF CHILDREN WITH HEMOPHILIA UNDER THE GENERAL ANESTHESIA : A CASE REPORT)

  • 김수경;박재홍;이긍호;김광철;최성철
    • 대한장애인치과학회지
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    • 제4권1호
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    • pp.7-11
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    • 2008
  • 치과의사는 혈우병 환자의 치료 시 주의사항에 대해 잘 알고 있어야 한다. 혈우병 환자의 내원 시 소아과 의사 및 혈우재단 등 전문가에게 의뢰하여 환자의 상태에 대한 자문을 얻어야 하고, 치과 치료 시 적절한 계획 하에 최소의 침습적인 치료가 행해지도록 노력해야 하며, 응급 상황을 대비하여 지혈방법을 습득해야 한다. 또 환자와 보호자에게 평소 구강 관리의 중요성을 일깨워 침습적 치과 치료의 빈도를 줄일 수 있도록 해야 한다.

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소아에서의 합병성 위십이지장 궤양 (A Clinical Analysis of Complicated Gastroduodenal Ulcer in Children)

  • 정광용;정연준;김찬영;양두현;김재천
    • Advances in pediatric surgery
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    • 제10권1호
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    • pp.22-30
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    • 2004
  • A total of 30 cases of the peptic ulcer in children, who underwent operations from January 1981 to December 1995 because of complications at Department of the Surgery, Chonbuk National University Medical School, is reviewed. Twenty-three were males (76.7%), 7 females (23.3%) and male was preponderant at 3.3:1. There were 25 cases (83.3%) age 10 to 15 years, 3(10.0%) between 2 and 9 years, and 2 (6.7%) below 2. The ulcer was located at duodenum in 27(90.0%), and at stomach in 3 cases (10.0%). Complications were perforation in 19 cases (63.3%), pyloric obstruction in 9 (30.0%) and bleeding in 2 (6.7%). For perforation, truncal vagotomy with pyloroplasty was done in 11 cases, truncal vagotomy with hemigastrectomy and gastrojejunostomy in 6, and simple closure in 2 cases. For obstruction, truncal vagotomy with hemigastrectomy and gastrojejunostomy was done in 5, and truncal vagotomy and pyloroplasty in 3 cases. For bleeding lesions, truncal vagotomy and pyloroplasty was performed in 2 cases. Ten postoperative complications developed in 9 patients: adhesive ileus in 5, recurrence in 2, pneumonia 2, and wound seroma 1 case. One patient developed a primary duodenal perforation and another a recurrent obstruction. Both of patients had symptoms for more than 3 years and were treated with truncal vagotomy and pyloroplasty for the primary operations. Hospital stay was 11.5 days for the patient with perforated ulcer, 11.0 days for the patient with pyloric obstruction, and 14.5 days for the child with bleeding. Average hospital period was 11.6 days. To reduce recurrences after operation, extensive procedure such as distal gastrectomy with vagotomy at the first operation should be considered in case with severe complication or with patients who have been symptomatic for long periods.

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근육내 자극요법 후 목빗근내 농양의 치험례 (A Case of Intramuscular Abscess in the Sternocleidomastoid Muscle after Intramuscular Stimulation (IMS))

  • 김재인;최환준;최창용;양형은
    • Archives of Plastic Surgery
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    • 제37권2호
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    • pp.182-186
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    • 2010
  • Purpose: Intramuscular stimulation (IMS) shows good results in the treatment of chronic pain patients who did not respond to other treatments such as oral analgesics, trigger point injection, nerve block and epidural steroid injection. But, IMS procedure especially, patients with diabetes mellitus (DM) has sometimes serious problem. So, we present a very rare case of intramuscular abscess in the sternocleiomastoid muscle after IMS with literature review. Methods: A 66 year old male visited our department 7 days after IMS in the neck. His premorbid conditions and risk factors of deep neck infection was DM and old age. Computed tomographic scans of the head and neck region were performed in this patient: signs of deep neck infection, were seen enhanced abscess in the sternocleidomastoid muscle, cellulitis overlying tissue of the neck, and air bubbles involved muscle. Necrotic wound was excised serially and we treated this with the Vacuum-assisted closure (VAC, Kinetics Concepts International, San Antonio, Texas) system device. After appropriately shaping the sponge in the pockets, continuous negative pressure of 125 mmHg was applied. The VAC therapy was utilized for a period of 12 days. Results: We obtained satisfactory results from wide excision, drainage of the abscess with the VAC system, and then primary closure. The postoperative course was uneventful. Results: We suggest that many of the infectious complications may be preventable by strict adherence to aseptic techniques and that some of the other complications may be minimized by refining the techniques with a clear understanding of the medical disorders of patients. And, the refined technique using the VAC system can provide a means of simple and effective management for the cervical intramuscular abscess, with better cosmetic and functional results.

외측광근을 이용한 대전자부 피부결손의 치료 (Vastus Lateralis Muscle or Myocutaneous Flap for Trochanteric Skin Defect)

  • 정성원;김종관
    • Archives of Reconstructive Microsurgery
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    • 제15권2호
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    • pp.65-69
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    • 2006
  • Purpose: We performed vastus lateralis (VL) muslce or myocutaneous flap for close of the trochanteric skin defect usually happened in paraplegia and report our 6 cases. Materials and Methods: Between March 2004 and August 2005 we performed 4 cases of VL muscule flap with skin graft and 2 cases of VL myocutaneous flap for close of the trochanteric skin defect in 6 paraplegia patients. There were 5 men and 1 woman and mean age was 52.2 years and mean diameters of skin defect was $8.3{\times}8.3\;cm$. The mean follow up period was 18 months. The survival of flap, complications, healing time and patient's satisfaction were evaluated. Results: All flaps were survived except 1 case of margin necrosis. In 2 cases, blood-serous discharges were continued after operation which might be due to dead space and treated with $2{\sim}3$ times debridement and delayed close. Mean time to heal the skin defect was 7.6 weeks. No infection and recurrence in follow up periods. Cosmetic results judged by patients are that 3 cases are good and 3 cases are fair. Conclusion: VL muscle and myocutaneous flap is good treatment method among the numerous methods in treatment-failed cases of trochanteric skin defects of paraplegia patients. This surgical procedure is simple, constant blood supply, good pliability, cosmetic results and also appliable to other skin defect of pelvis girdle like sacrum and ischium.

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