• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1125-1129
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• 2005

Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology ($79\%$ identity, $88\%$ similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. The serine, histidine, and aspartic acid residues composing the catalytic triad of typical serine proteases are also well conserved. When the trypsin activity of the SprU was spectrophotometrically measured by the enzymatic hydrolysis of the artificial chromogenic substrate, N-${alpha}$-benzoyl-DL-arginine-p-nitroanilide, the S. lividans transformant with pWHM3-U gave 3 times higher activity than that of control. When the same recombinant plasmid was introduced into S. griseus, however, the gene dosage effect was not so significant, as in the cases of other genes encoding serine proteases, such as sprA, sprB, and sprD. Although two trypsins, SprU and SGT, have a high degree of homology, the pI values, the gene dosage effect in S. griseus, and the gene arrangement adjacent to the two genes are very different, suggesting that the biochemical and biological function of the SprU might be quite different from that of the SGT.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.373-381
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• 2020

There is a need for newer feed additives due to legal prohibition on inclusion of growth promoting antibiotics in livestock diets in several countries due to antimicrobial resistance. In this context, rare earth elements (REE) have gained attention among animal nutritionists as potential growth promoters. Currently, several studies have reported better weight gain, milk production, egg laying capacity and feed conversion efficiency among different breeds of farm animals following supplementation with REE, with however largely inconsistent results. Furthermore, REE supplementation has also shown to improve ruminal fibrolytic and proteolytic activities as well as flavor of meat with negligible residues in edible tissue, however the mechanism behind this action is still unclear. According to existing research, due to their poor absorption and similarity with calcium REE might exert their action locally on gut microbial populations within the gastrointestinal tract (GIT). Moreover, REE have also shown anti-inflammatory, anti-oxidative as well as immune stimulating effects. The present review aims to broaden the knowledge about use of REE as feed additives for livestock and sum up efficacy of REE supplementation on performance and health of animals by comparing the findings. Till date, researches with REE have shown properties that make them a promising, new and safe alternative feed additive but further exploration is recommended to optimize effects and clarify discrepancy of various results before practical proposals can be drafted.

• 한국정보통신학회논문지
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• 제19권3호
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• pp.543-551
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• 2015

인터넷과 모바일 기기의 사용이 보편화되면서 사용자들이 다양한 웹 사이트에서 자신이 원하는 정보를 찾기 위해 검색과 추천을 이용하는 것이 일상화되고 있다. 본 논문에서는 사용자에게 보다 적합한 아이템을 추천하기위해 사용자의 활동과 시간 정보를 적용하여 시간의 흐름에 따른 사용자의 선호도 변화를 반영한 추천 기법을 제안한다. 제안하는 기법은 아이템 선택 시 고려되는 태그 정보를 포함한 데이터를 카테고리별로 분류한 후 시간 변화에 따른 사용자 선호도 변화 정보를 반영한 데이터만을 사용한다. 해당 카테고리를 선호하는 사용자에게는 협업 필터링 기법에 태그 정보를 적용하여 추출한 아이템을 추천하고, 일반 사용자에게는 태그 정보를 사용하여 계산한 순위를 기반으로 아이템을 추천한다. 제안하는 기법은 hetrec2011-movielens-2k 데이터셋을 사용하여 실험하였으며 실험을 통해 제안한 기법이 기존의 아이템 기반, 사용자 기반 기법보다 추천의 정확성과 적합성이 향상되는 것을 확인하였다.

• 대한가정학회지
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• 제27권1호
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• pp.39-46
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• 1989

Mung bean starch gel (Mook) and gel made from starch of cowpea are similar properties in texture. In order to elucidate the similarity between these two starch gels, some physicochemical properties of cowpea starch were compared with those of mung bean starch. Water bildings capacity of cowpea starch (183.6%) was a little low than that of mung bean starch (184.2%). The solobility, swelling power and optical transmitance of the cowpea starch showed a smiliar pattern to mung bean starch, but cowpea starch had a little lower solubility than mung bean starch. Amylogram of mung bean strach (4, 5, 6, 7%) shoved no peak viscosity but cowpea starch (4, 5, 6%) showed peak viscosity and both starches showed high viscosities when cooling. Cowpea and mung bean starches had the blue value of 0.41 and 0.47, the alkali number of 8.4 and 8.0, the amylose content of 30.5 and 32.1%, the molecular weight of amylose of 30,000 adn 29,258 and glucose unit per segment of amylopectin of 27.6 and 26.8 respectively. The shape of cowpea and mung bean strach granules were round and elliptical, and the mean vlalue of major axis, minor axis and the ratio of these were 20.7 and 21.8 ${\mu}{\textrm}{m}$, 14.6 and 14.4 ${\mu}{\textrm}{m}$ and 1.42 and 1.51, respectively. The extent of retrogradation determined by the glucoamylase digestion method and syneresis showed that cowpea starch gel was larger than that syneresis showed than cowpea starch gel was larger than that of mung bean starch gel. The redults of X-ray diffraction studies showed A pattern for two starches, Diffraction peak of gels disappeared with gelatinization of starches but that of two starch gels storaged for 2 days at 5$^{\circ}C$ showed a similar patterm.

• Composites Research
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• 제31권5호
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• pp.215-220
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• 2018

본 연구에서는 적층 패턴이 다른 5가지 섬유금속적층판(Fiber Metal Laminates, FMLs)에 대한 유한요소해석을 수행하여 선행 연구로 수행한 낙추충격시험과의 유사성을 검증하였고, 효과적인 저속 충격 해석 모델을 개발하였다. 또한 동일한 두께를 가지는 연강(mild steel)과 에너지흡수율을 비교하여 Carbon-Steel 섬유금속적층판의 내충격성을 확인하였다. Carbon-Steel 섬유금속적층판은 동일한 두께를 가지는 연강에 비하여 우수한 충격흡수율을 보였으며, 모든 적층 패턴에서 96% 이상의 높은 에너지흡수율 갖는 것을 확인하였다. 본 연구에서 제시한 저속 충격 해석 모델은 복합형상 및 자동차 구조체 연구에 효과적으로 적용할 수 있을 것으로 판단된다.

• 대한토목학회논문집
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• 제33권4호
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• pp.1693-1705
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• 2013

국내의 수로교는 쌀문화로 상징되는 농업용수를 공급하는 교량으로서 수로교를 개보수하기 위해서는 기본설계를 실시하는 것이 바람직하나 현재 생략되고 있는 실정이므로 이에 소요되는 공사비를 산정할 필요가 있다. 이 연구에서는 2003년 이후 교체한 RC구조 수로교에 대한 실적자료를 기초로 개략공사비 산정 회귀분석(RA) 모델과 사례기반추론(CBR) 모델을 개발하였다. RA 모델의 경우 단순회귀 모델이 다중회귀 모델보다 오차율이 낮았다. CBR 모델의 경우 유전 알고리즘을 이용하였으며 영향요인의 가중치, 편차, 순위조건을 최적화 대상으로 하였고 특히 영향요인 가중치의 범위를 제한하여 수로교 개보수 공사비의 예측 정확도를 제고하였다. RA 모델과 CBR 모델 사이의 오차율은 통계적 차이를 보이지 않았다. 본 논문에서 제시된 수로교 개보수 개략공사비 산정방법은 개보수사업의 시행에 따른 신속한 의사결정을 하는데 활용될 수 있을 것으로 기대된다.

• Toxicological Research
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• 제26권1호
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• pp.47-52
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• 2010

The Malassezia fungi are responsible for various human skin disorders including dandruff and seborrheic dermatitis. Of the Malassezia fungi, Malassezia globosa (M. globosa) is one of the most common in human scalp. The completed genome sequence of M. globosa contains four putative cytochrome P450 genes. To determine the roles of Malassezia P450 enzymes in the biosynthesis of ergosterol, we isolated MGL3996 gene from M. globosa chromosomal DNA by PCR. The MGL3996 gene encodes an enzyme of 616 amino acids, which shows strong similarity with known CYP52s of other species. MGL3996 gene was cloned and expressed in Pichia pastoris (P. pastoris) heterologous yeast expression system. Using the yeast microsomes expressing MGL3996 protein, a typical P450 CO-difference spectrum was shown with absorption maximum at 448 nm. SDS-PAGE analysis revealed a protein band of apparent molecular weight 69 kDa and Western blot with anti-histidine tag antibody showed that MGL3996 was successfully expressed in P. pastoris. Cloning and expression of a new P450 gene is an important step to study the P450 monooxygenase system of M. globosa and to understand the role of P450 enzymes in pathophysiology of dandruff.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제21권4호
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• pp.278-288
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• 2018

Purpose: Dehydration is a paediatric medical emergency but there is no single standard parameter to evaluate it at the emergency department. Our aim was to evaluate the reliability and validity of capillary refilling time as a triage parameter to assess dehydration in children. Methods: This was a prospective pilot cohort study of children who presented to two paediatric emergency departments in Italy, with symptoms of dehydration. Reliability was assessed by comparing the triage nurse's measurements with those obtained by the physician. Validity was demonstrated by using 6 parameters suggestive of dehydration. Comparison between refilling time (RT) and a validated Clinical Dehydration Score (CDS) was also considered. The scale's discriminative ability was evaluated for the outcome of starting intravenous rehydration therapy by using a receiver operating characteristic (ROC) curve. Results: Participants were 242 children. All nurses found easy to elicit the RT after being trained. Interobserver reliability was fair, with a Cohen's kappa of 0.56 (95% confidence interval [CI], 0.41 to 0.70). There was a significant correlation between RT and weight loss percentage (r-squared=-0.27; 95% CI, -0.47 to -0.04). The scale's discriminative ability yielded an area under the ROC curve (AUC) of 0.65 (95% CI, 0.57 to 0.73). We found a similarity between RT AUC and CDS-scale AUC matching the two ROC curves. Conclusion: The study showed that RT represents a fast and handy tool to recognize dehydrated children who need a prompt rehydration and may be introduced in the triage line-up.

• Journal of Life Science
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• 제9권2호
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• pp.9-14
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• 1999

Pseudomonas iodinum IFO 3558 adenosine deaminase(ADA) gene was cloned by the polymerase chain reaction and deduced the amino acid sequence of the enzyme. DNA sequence homology of Pseudomonas iodinum IFO 3558 ADA gene was compared to those of E. coli, human and mouse ADA genes. Unambiguous sequence from both strands of pM21 was obtained for the region believed to encode ADA. The sequence included a 804-nucleotide open reading frame, bounded on one end by sense primer and on the other end by two antisense primer. This open reading frame encodes a protein of 268 amino acids having a molecular weight of 29,448. The deduced amino acid sequence shows considerable similarity to those of E. coli, mouse and human ADA. Pseudomonas iodinum IFO 3558 nucleotide sequence shows 98.5% homology with that of the E. coli ADA sequence and 51.7% homology with that of the mouse ADA sequence and 52.5% homology with that of the human ADA sequence. The ADA protein sequence of Pseudomonas iodinum IFO 3558 shows 96.9% homology with that of the E. coli and 40.7% homology with that of the mouse and 41.8% homology with that of the human. The distance between two of the conserved elements, TVHAGE and SL(1)NTDDP has veen exactly conserved at 76 amino acids for all four ADAs. Two of the four conserved sequence elements shared among the four ADAs are also present in the yeast, rat, human (M), and Human(L) AMP deaminase. The SLSTDDP sequence differs only in the conservative substitution of a serine for an asparagine. A conserved cysteine with conserved spacing between these two regions is also found. Thus, sequence analysis of four ADAs and four AMP deaminases revealed the presence of a highly conserved sequence motif, SLN(S)TDDP, a conserved dipeptide, HA, and a conserved cysteine residue.

• 한국의류학회지
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• 제33권11호
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• pp.1781-1793
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• 2009

This study classifies the lifestyle of elderly female consumers in their 60's and 70's and examines the influence of lifestyles on personal clothing purchase behavior. This paper classified the types of lifestyle and examined the influence of lifestyle types on the perceived importance of salesperson's characteristics and store evaluation criteria. A survey was conducted on 194 elderly females in their 60's and 70's living in Seoul. The SPSS 14.0 program was used to analyze the data. Descriptive statistics, factor analysis, reliability analysis, K-means cluster analysis, and one-way ANOVA followed by Duncan post hoc comparisons were conducted. The research results are as follow. First, in order to identify the lifestyle factors of elderly female consumers a factor analysis was conducted that indicated 9 factors. Second, as a result of the cluster analysis of lifestyle types, the respondents were classified into 'proactives', 'passive stagnants', 'independent misers', and 'conservative consumers'. Third, according to lifestyle, weight on 'appearance and image', 'professionalism', 'ethics', 'similarity', and 'customer orientation' abilities of the salesperson were significantly different. Fourth, in relation to the store evaluation criteria by lifestyle, a significant difference was found in the attention to 'physical service' by a salesperson.