• The Transactions of The Korean Institute of Electrical Engineers
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• v.60 no.12
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• pp.2352-2360
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• 2011

In this paper, we introduce a comprehensive design methodology of Radial Basis Function Neural Networks (RBFNN) that is based on mechanism of clustering and optimization algorithm. We can divide some clusters based on similarity of input dataset by using clustering algorithm. As a result, the number of clusters is equal to the number of nodes in the hidden layer. Moreover, the centers of each cluster are used into the centers of each receptive field in the hidden layer. In this study, we have applied Fuzzy-C Means(FCM) and K-Means(KM) clustering algorithm, respectively and compared between them. The weight connections of model are expanded into the type of polynomial functions such as linear and quadratic. In this reason, the output of model consists of relation between input and output. In order to get the optimal structure and better performance, Particle Swarm Optimization(PSO) is used. We can obtain optimized parameters such as both the number of clusters and the polynomial order of weights connection through structural optimization as well as the widths of receptive fields through parametric optimization. To evaluate the performance of proposed model, NXT equipment offered by National Instrument(NI) is exploited. The situation awareness system-related intelligent model was built up by the experimental dataset of distance information measured between object and diverse sensor such as sound sensor, light sensor, and ultrasonic sensor of NXT equipment.

• Journal of the Institute of Electronics and Information Engineers
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• v.54 no.4
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• pp.61-67
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• 2017

Low-dose X-ray fluoroscopic image sequences to avoid radiation exposure risk are contaminated by quantum noise. To restore these noisy sequences, we propose a 3D nonlocal means (NLM) filter based on stochastic distancesed can be applied to the denoising of X-ray fluoroscopic image sequences. The stochastic distance is obtained within motion-compensated noise filtering support to remove the Poisson noise. In this paper, motion-adaptive weight which reflected the frame similarity is proposed to restore the noisy sequences without motion artifact. Experimental results including comparisons with conventional algorithms for real X-ray fluoroscopic image sequences show the proposed algorithm has a good performance in both visual and quantitative criteria.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.344-350
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• 2006

Thermoplasma acidophilum is a thermoacidophilic archaeon that grows optimally at $59^{\circ}C$ and pH 2. Along with another thermoacidophilic archaeon, Sulfolobus solfataricus, it is known to metabolize glucose by the non-phosphorylated Entner-Doudoroff (nED) pathway. In the course of these studies, the specific activities of glyceraldehyde dehydrogenase and glycerate kinase, two enzymes that are involved in the downstream part of the nED pathway, were found to be much higher in T. acidophilum than in S. solfataricus. To characterize glycerate kinase, the enzyme was purified to homogeneity from T. acidophilum cell extracts. The N-terminal sequence of the purified enzyme was in exact agreement with that of Ta0453m in the genome database, with the removal of the initiator methionine. Furthermore, the enzyme was a monomer with a molecular weight of 49kDa and followed Michaelis-Menten kinetics with $K_m$ values of 0.56 and 0.32mM for DL-glycerate and ATP, respectively. The enzyme also exhibited excellent thermal stability at $70^{\circ}C$. Of the seven sugars and four phosphate donors tested, only DL-glycerate and ATP were utilized by glycerate kinase as substrates. In addition, a coupled enzyme assay indicated that 2-phosphoglycerate was produced as a product. When divalent metal ions, such as $Mn^{2+},\;CO^{2+},\;Ni^{2+},\;Zn^{2+},\;Ca^{2+},\;and\;Sr^{2+}$, were substituted for $Mg^{2+}$ the enzyme activities were less than 10% of that obtained in the presence of $Mg^{2+}$. The amino acid sequence of T. acidophilum glycerate kinase showed no similarity with E. coli glycerate kinases, which belong to the first glycerate kinase family. This is the first report on the biochemical characterization of an enzyme which belongs to a member of the second glycerate kinase family.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.530-541
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• 2014

The dredge gear is dragged along the bottom of the sea to catch targeted edible bottom dwelling species. Species composition and ecological index of the catches of dredge were estimated around Gangneung coastal fishing ground by dredge with different mesh size and tooth space from July to December 2013. Eight different types of dredge including four different tooth space (24.7 mm, 29.9 mm, 34.9 mm, 40.1 mm) and four different mesh size(15.5 mm, 32.7 mm, 51.1 mm, 60.0 mm) were used in the experiment. During the experiment, total catches were collected 31 species as sipunculida 1 species, mollusca 13 species, annelida 3 species, arthropoda 8 species, echinodermata 4 species and others 2 species. The dominant genus were mollusca and echinodermata while the dominant species were Megangulus venulosus, Pseudocardium sachalinensis, Schaphechinus brevis. The richness index was ranged 1.29-1.72, evenness index was 0.6-0.65 and diversity index was 1.65-1.83 according to the tooth space and mesh size of dredge. Richness index, diversity index were high at tooth space 34.9 mm dredge and ecological index showed decreasing tendency with the increasing of mesh size of dredge. Cluster and MDS analysis, based on a Bray-Curtis and similarity matrix of fourth root transformed data of number of species and wet weight, showed division into four different groups as four different tooth space (Group A), four different mesh size Group B (51.1 mm, 60.0 mm), Group C (32.7 mm) and Group D (15.5 mm).

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.391-397
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• 2014

Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a minei-mpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn ($170.50mg\;kg^{-1}$), As ($114.05mg\;kg^{-1}$), Zn ($92.07mg\;kg^{-1}$), Cu ($62.44mg\;kg^{-1}$), and Pb ($40.29mg\;kg^{-1}$). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

• Korean Journal of Microbiology
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• v.28 no.2
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• pp.128-133
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• 1990

The chromatin of all higher eukaryotic cells contains a group of very basic low-mole-cular weight proteins, the histones. But much less is known about histones in lower eukaryotes. Our purpose was to study the histones of the genus Rhizopus. After isolation and purification of nucleoprotein the basic nucleoproteins were analyzed by gel electrophoresis, in sodium dodecyl sulfate as well as acid/urea gels and compared with calf thymus histones. Their electrophoretic mobility in polyacrylamide gel indicate that they are histone homologous, although not identical, to the H2A, H2B, H3 and H4 histones of mammals with the exception of H1. The result suggests that Rhizopus thus appears to contain histone proteins which are homologous to the histones from in higher eukaryotes. The similarity between the calf thymus histone H1 and the Rhizopus high band group remains to be discussed.

• Journal of Korea Multimedia Society
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• v.12 no.11
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• pp.1692-1700
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• 2009

This paper proposes the direction distribution of surface normal vectors as a feature descriptor of three-dimensional models. Proposed the feature descriptor handles rotation invariance using a principal component analysis(PCA) method, and performs mesh simplification to make it robust and nonsensitive against noise addition. Our method picks samples for the distribution of normal vectors to be proportional to the area of each polygon, applies weight to the normal vectors, and applies interpolation to enhance discrimination so that the information on the surface with less area may be less reflected on composing a feature descriptor. This research measures similarity between models with a L1-norm in the probability density histogram where the distances of feature descriptors are normalized. Experimental results have shown that the proposed method has improved the retrieval performance described in an average normalized modified retrieval rank(ANMRR) by about 17.2% and the retrieval performance described in a quantitative discrimination scale by 9.6%~17.5% as compared to the existing method.

• Applied Biological Chemistry
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• v.30 no.4
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• pp.305-310
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• 1987

The enzymatic properties of ${\beta}-amylase$ from soybean and sweet potato were compared. The sweet potato enzyme consists of four identical subsunits whereas soybean enzyme has no subunit $structure^{12,\;15)}$. In the denatured state, both enzymes exhibited the same molecular weight on SDS-gel electrophoresis and on gel-filtration analysis. The spectra of circular dichroism revealed that both enzyme have almost same secondary structure but the environment of aromatic side chains are different. The chemical cleavage of soybean and sweet potato ${\beta}-amylases$ at cysteine residues and methionine residues demonstrated the homology of amino acid sequence between the enzymes. The similarity between soybean and sweet potato ${\beta}-amylase$ was also revealed by immunological method. The antibody for soybean enzyme inhibited the activity of sweet potato enzyme but it did not inhibit the activity of wheat, barley and Japanese-raddish ${\beta}-amylases$.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1753-1759
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• 2006

Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ionexchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and $50^{\circ}C$. The $K_m\;and\;V_{max}$ values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1125-1129
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• 2005

Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology ($79\%$ identity, $88\%$ similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. The serine, histidine, and aspartic acid residues composing the catalytic triad of typical serine proteases are also well conserved. When the trypsin activity of the SprU was spectrophotometrically measured by the enzymatic hydrolysis of the artificial chromogenic substrate, N-${alpha}$-benzoyl-DL-arginine-p-nitroanilide, the S. lividans transformant with pWHM3-U gave 3 times higher activity than that of control. When the same recombinant plasmid was introduced into S. griseus, however, the gene dosage effect was not so significant, as in the cases of other genes encoding serine proteases, such as sprA, sprB, and sprD. Although two trypsins, SprU and SGT, have a high degree of homology, the pI values, the gene dosage effect in S. griseus, and the gene arrangement adjacent to the two genes are very different, suggesting that the biochemical and biological function of the SprU might be quite different from that of the SGT.