• 생명과학회지
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• 제18권9호
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• pp.1186-1193
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• 2008

방글라데시 미멘싱에 소재하는 양식어장에서 pangasiid catfish (Pangasius hypophthalmus), silver carp (Hypophthalmichthys molitrix)와 catla (Catla catla)의 조합과 혼합비를 달리하여 16주간 복합양식하였을 때, 이들 어종의 성장률을 조사하였다. 헥타르당 30,000개체의 밀도로 복합양식하여 성장률을 측정하였으며, 혼합조성비는 다음과 같다: 실험구(treatment) 1은 pangasiid catfish와 silver carp을 1:1비율로, 실험구 2는 pangasiid catfish와 cata를 1:1비율로, 실험구 3과 4는 pangasiid catfish, silver carp과 catla를 각각 2:1:1과 3:2:1비율로 복합양식하였다. 양식환경에 영향을 주는 수온, 투명도, 용존산소량, pH, $NO_{3}-N$, $PO_{4}-P$, 엽록소 a와 같은 몇몇 물리화학적, 생물학적 요인과 플랑크톤의 군집을 정기적으로 조사하였다. 어류의 성장속도는 비 성장율(specific growth rate), 일간 체중 증가(daily weight gain)와 체중증가 비율(percent weight gain)을 측정하였다. Pangasiid catfish의 성장률과 사료이용률은 모든 실험구에서 유사하게 나타났다. Silver carp의 성장률은 모든 실험구에서 유의한 차이를 나타내었는데(p<0.05), 양식밀도가 증가할수록 성장률이 현저하게 감소하였으며, Silver carp의 밀도가 가장 낮은 복합양식 조성인 pangasiid catfish, silver carp과 catla의 비율이 각각 2:1:1일 때, silver carp의 성장률이 가장 높게 나타났다. Silver carp의 밀도가 증가할수록 catla의 성장률이 현저하게 감소하는 것으로 나타나, catla의 성장률은 silver carp의 유무에 영향을 받는 것으로 판단되었다. Catla의 성장률은 pangasiid catfish와 calta의 비율이 1:1일 때, 가장 높은 것으로 나타났다. 양식수율과 경제적 이익은 실험구에 따라 현저하게 다른 양상을 보였다(p<0.05). 가장 높은 양식수율과 경제적 이익은 pangasiid catfish, silver carp과 catla를 각각 2:1:1 비율로 양식한 결과에서 얻어졌는데, 이는 silver carp의 높은 성장률과 catla의 시장 단가가 높은 것에 기인하는 것으로 나타났다. 모든 실험구의 수질 상태는 양식기간 동안 적절한 수준을 유지하였으며, 각각의 값은 모든 실험구에서 뚜렷한 차이를 보이지 않았다(p<0.05).

• BMB Reports
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• 제41권3호
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• pp.204-209
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• 2008

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins.

• 한국어병학회지
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• 제34권2호
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• pp.169-176
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• 2021

Parasitic infection is among the most common problems for carp cultivation. They are also important for the principal entrance of other hazardous infections as well. This study was carried out for determining of parasitic fauna of two major carp known as silver and grass carp with the comparison of prevalence value and intensity rate of parasites among them, alongside the relationship between the biometric characteristics and host sex with the infection level. For this purpose, a total of 94 fish samples were caught randomly using a fishing net, from Guilan ponds during spring and summer of the year 2018 and transported alive to the laboratory. Upon arriving, the biometric characteristics and genus of each carp were measured individually. Specimens were then acquired from the skin, gills, and eyes of the carp and examined according to standard parasitology methods. Recovered parasites were observed under a light microscope and then fixed for identification. As the result, the occurrence and intensity in the higher length group were comparatively greater than the lower one. Also, the prevalence and intensity of total parasites in male carp were higher than in females. In this research, Dactylogyrus hypophthalmichthys and Dactylogyrus aristhichtys were observed in silver carp and Dactylogyrus lamellatus was detected in grass carp. In the paper below, we found that the host specificity varies in different species of Dactylogyrus isolated from grass carp and silver carp.

• 생태와환경
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• 제33권4호통권92호
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• pp.319-327
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• 2000

수심이 얕은 부영양호수에서 우점하는 식물플랑크톤에 대한 1년생 백련어의 섭식특성을 조사하고자, 1997년 5월 23일부터 9월 18일까지 호수의 연안에 4개의 enclosure를 설치하고, 어류투입 이후의 각 enclosure수중과 어류의 장 내용물 중의 식물플랑크톤 군집의 변화를 비교하였다. 조사기간동안 각 enclosure의 수온, 플랑크톤의 총생물량, 어류 아가미의 여과공 등은 시간에 따라 른 변화가 없었으며, 어류성장은 주로 투입된 어류의 밀도에 의존되었다. 어류의 내용물에 의하면, 저밀도 투입 enclosure에서 수중과 어류 전장간의 플랑크톤군집의 유사도가 높게 나타났으며(p<0.05), 전체적으로 모든 enclosure에서 크기가 큰(>$100,000{\mu}m^3$) 식물플랑크톤-Oscillatoria, Anabaena, Melosira등은 어류투입이후 크게 감소되지 않았다. 어류의 먹이선택지수(${\alpha}$)와 플랑크톤의 크기 (Ingestion unit)사이에도 매우 낮은 상관성을 보였다(r = 0.001, p>0.5). 식물플랑크톤의 분석결과, 백연은 남조나 녹조에 비해 규조를 더 선호하였는데, 이는 규조의 세포벽이 다른 조류에 비해 소화과정동안 쉽게 파괴되지 않고, 저배율의 현미경적시야에서도 간단히 확인된다는 점 등,선택지수를 과대평가하게 되는 단점이 있기 때문에 백련어의 먹이습성에 대한 방법론적 개선이 필요하다고 판단된다.

• Fisheries and Aquatic Sciences
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• 제11권1호
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• pp.15-22
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• 2008

Excessive growth of phytoplankton is a common and severe problem in intensively farmed pangasiid catfish (Pangasius hypophthalmus) culture ponds. It can lead to cyanobacterial blooms, reduced fish growth, bad-tasting fish flesh, and lower market demand. To investigate how to manage undesirable phytoplankton growth, we evaluated three stocking strategies in nine rural fishponds (0.020-0.022 ha) owned by various farmers: a pangasiid catfish mono culture (treatment 1, $T_1$), and pangasiid catfish-silver carp (Hypophthalmichthys molitrix) polycultures at two stocking ratios of 1:1 (treatment 2, $T_2$) and 2:1 (treatment 3, $T_3$). The total density of all ponds was approximately 30,000 fishes/ha. Monoculture ($T_1$) resulted in significantly higher (p < 0.05) nutrient levels (nitrate and phosphate) in ponds than did polyculture ($T_2$ and $T_3$). Nutrient loads increased with culture time, resulting in increased growth of phytoplankton, including Cyanophyceae (9 genera), Chlorophyceae (15 genera), Bacillariophyceae (8 genera), and Euglenophyceae (3 genera). The introduction of silver carp as a co-species helped to regulate phytoplankton growth and to improve the water quality of pangasiid catfish culture ponds.

• BMB Reports
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• 제39권4호
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• pp.346-354
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• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.

• 한국어병학회지
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• 제30권2호
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• pp.135-148
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• 2017

Pathogen introductions associated with aquatic invasive species threaten ecosystems and biodiversity worldwide. Bigheaded carps (BHC), including Silver Carp Hypophthalmichthys molitrix, Bighead Carp H. nobilis, and their hybrids, are prolific, invasive pests in central US rivers. However, little is known about pathogen effects on invading BHC or how BHC affect the disease risk profile for native fishes in receiving ecosystems. We therefore conducted, from May 2013-December 2014, a systematic pathogen survey for BHC and native fishes in the Wabash River watershed, Indiana, USA. We found Pseudomonas fluorescens, P. putida, and Salmonella enterica DNA in BHC as well as native fishes, although none of these bacteria were exclusively present in BHC. DNA from other bacterial taxa was detected only in native fishes and Common Carp Cyprinus carpio. No gastrointestinal helminths were detected in BHC, although they were common in most native fishes examined. We also conducted in vitro studies on BHC tissues (skin, gill, fin, and fry) and found high sensitivity to Largemouth Bass virus, viral hemorrhagic septicemia virus, and infectious pancreatic necrosis virus. We conclude that BHC are not heavily burdened by bacteria, viruses and parasites in the invaded study ecosystems, although they do harbor native bacteria and show potential for high sensitivity to endemic viruses.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7879-7884
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• 2014

To investigate the association between intake of freshwater fish and their fatty acids and the risk of breast cancer in Chinese women, we conducted a case-control study with 669 cases and 682 population-based controls in Jiangsu Province of China. A structured questionnaire was used to elicit detailed information. Unconditional logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Total freshwater fish intake was linked to decrease in the adjusted OR for breast cancer, but without dose-dependence. Analyses by freshwater fish species showed that consumption of black carp and silver carp was inversely related to breast cancer risk, with adjusted-ORs for the highest intake category of black carp (${\geq}500g/month$) of 0.54 (95%CI=0.33-0.92; $P_{trend}$ <0.002) and for silver carp (${\geq}1000g/month$) of 0.19 (95%CI=0.11-0.33; $P_{trend}$ <0.001). In contrast, consumption of crucian carp was positively related to breast cancer risk, with an adjusted OR for the highest intake category (${\geq}1000g/month$) of 6.09 (95%CI=3.04-12.2; $P_{trend}$ <0.001). Moderate intakes of SFA, PUFA, n3-PUFA and n6-PUFA from freshwater fish may decrease the risk of breast cancer among premenopausal women. The findings of this study suggest that intake of freshwater fish and their fatty acids may modify risk of breast cancer, and that different species of freshwater fish could have a different actions on breast cancer risk. Future epidemiologic studies are needed to know the effects of freshwater fish intake on breast cancer risk and the cause of these effects.

• Environmental Analysis Health and Toxicology
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• 제12권3_4호
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• pp.15-22
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• 1997

This experiment was performed to examine the immuno-reactive characteristics of fish metal-binding protein, metallothionein (MT), and gain the practical understandings for the proposed use of fish MT as a biomarker. Liver MT induced by Cd in the silver carp was seperated and purified by gel filtration chromatography and ion exchange chromatography. The immuno-reactivity of fish MT was examined with 3 rabbit antisera. Fish MT showed little reactivity with rabbit anti-rat MT antiserum and a weak reactivity with anti-MT peptide antiserum while showed a strong reactivity with rabbit anti-fish MT antiserum. The time-course change of liver MT in the silver carp, after waterborne exposure to 1 ppm of Cd, was checked by Cd-hem method and established competitive ELISA. In both cases, the induction of liver MT showed a good increasing relationship with the exposure days. The results indicate that the fish MT can be developed as a useful biomonitoring means in the toxicological study and for the evaluation of water pollution.

• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.11-17
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• 1996

The purpose of this experiment is to estabilish the sensitive and specific ELISA (enzyme linked immunosorbent assay) system for the detection of fish metallothionein (MT). Silver carp were injected with CA of 1-8mg/kg body wt. 4 times during 10 days. Silver carp was very tolerant species to CA. Cd induced MT in liver was seperated and purified by gel filtration chromatography and ion exchange chromatography and identified by spectrophotometry, native gel electrophoresis and western blot analysis. The rabbit antiserum was produced by immunizing rabbit with lyophilized MT, and the competitive ELISA system was estabilished for the detection of fish MT. In the present ELISA system, the detection limit was about 33 ng/ml. When this ELISA system was employed to determine the MT level in the supernatant sample of fish liver homogenate, the reaction curve showed a good parallel corelationship with the calibration curve over a certain dilution range. The results indicate that the competitive ELISA can be a useful tool for the detection of fish MT in the toxicological study and the evaluation of water pollution.