• Journal of Life Science
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• v.28 no.11
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• pp.1316-1320
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• 2018

The effects of a green tea extract (GTE) were examined using the MCF-7 human breast cancer cell line. Cell viability assays using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed that GTE had a significant cytotoxic effect on MCF-7 cells, depending on the concentration of GTE. Western blotting of p53 and its related proteins, p21/cip1 and CDK2, after GTE treatment revealed that a significant and concentration dependent increase in p53 protein in response to GTE. The levels of p21/cip1 proteins were also increased at low GTE concentrations were significantly increased even at the highest GTE concentrations. However, the level of CDK2 was significantly decreased by treatment with high concentrations of GTE. These results indicate that treatment with GTE increased the p53 level in MCF-7 cells, and this activation of p53 markedly elevated the levels of p21/cip1proteins, which, in turn, inhibited CDK2 expression in the MCF-7 cells. The inhibition of CDK2 expression might then affect cell cycle progression. Subsequent FACS analysis indicated that GTE treatment the gradually increased progression of the MCF-7 to the G1 phase. These results clearly demonstrate that the anti-tumor effect of GTE in MCF-7 cells is regulated by p53 arrest of the MCF-7 cells at the G1 stage of cell cycle.

• Korean Journal of Heritage: History & Science
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• v.45 no.4
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• pp.92-103
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• 2012

The Cheomsungdae was built in the Silla dynasty during the reign of queen Seondeok. It has a round cylindrical structure with a flowing curved fa ade. The identity of the Cheomsungdae has not been revealed since there is not much historical evidence or documents about the building. This study is trying to investigate the building technique and method from the technical point of view of the past when it was constructed. There have been much work and studies done for the Cheomsungdae, but not much were focusing on the technical aspects of the building. In addition there are many questions and doubts about the hypothesis of the building technique of Cheomsungdae since there aren't any remaining documents or historical evidence supporting it. Among many questions, we think that the discussion on falsework technique is not considering traditional construction method of the Dujabee (a traditional construction technique using various tools and equipment for the stability of the building) technique. Therefore, it is hard to identify them as reliable historical facts. As the result of the study, we want to provide the basic data on the construction techniques of Korean traditional architecture and broaden the study scope of technical history by narrowing the errors. The study could be summarized into three points. 1. The historical architecture Cheomseongdae was constructed by using traditional crane techniques such as a Noklo (pulley ladder). Cheomseongdae was re-evaluated as a high level technology for the history of architecture. 2. The benchmark method on Cheomseongdae construction has been applied with a precise scientific method based on the geometrical principals using the central axis. 3. In terms of the history of Korean traditional architecture technology, as there aren't many studies done we proposed various basic data for the traditional crane techniques and criteria of Korean traditional architecture technology. We could expect various and active studies for the technical approach of the history of architecture.

• Korean Journal of Heritage: History & Science
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• v.42 no.3
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• pp.176-191
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• 2009

This report is a result of investigating the materials and techniques of the lacquer ware excavated from Hwangnamdaechong Tomb known as an old tomb in the Gyeongju maximum district. First, the fragment of lacquer ware was classified by the shape of it. Next, the thin section of lacquer film (all sides about 2mm) was made based on the result. Then they were observed under the microscope, and classified the kind of the foundation mixture and the applied structure. It is a result as follows. 1 The bone dust was mixed by the foundation of the made of cloth-body lacquer ware such as eared lacquer cup, lacquer bowl, and the lacquer rectangular containers excavated from the south tomb. 2 Eared lacquer cup and lacquer bowl, and rectangular box excavated from south and north tomb had the possibility they were the set of articles partially of tableware and the cosmetic box. 3 There is a difference in the quality of burial lacquer ware between in south tomb and north tomb. A lot of highlevel lacquer ware such as eared lacquer cup, lacquer bowl and the rectangular lacquer containers were discovered from the south tomb. 4 Many of containers where animal and bird had been drawn on the black lacquered ground excavated from north tomb were wooden bath.

• Korean Journal of Heritage: History & Science
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• v.46 no.1
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• pp.140-159
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• 2013

The wooden stupa of Hwangyongsa temple - which was designed and constructed by master craftsman Abigi of the Baekje Dynasty during the Silla Dynasty between the 12th year of Queen Seondeok (AD 643) and AD 646 - can be regarded as a typical Korean wooden stupa. However, it was destroyed by fire during an invasion by the Mongolians in AD 1238 during the Koryo Dynasty. In spite of progress in research for the restoration of the wooden stupa, there are some remains to be re-considered in regard to ruins, relics and literature previously discovered. In particular, research conducted on frame construction and structure can be considered an important basis for restoration. However, previous studies seem to have not attempted to establish structural and formative characteristics of the wooden stupa based on the indigenous techniques and styles of traditional Korea, but general characteristics of the wooden stupa biased toward cultural interchange with neighbouring countries. As such, in this study the frame construction and structure of the wooden stupa of Hwangyongsa temple were analysed and considered in detail based on the re-interpretation of literature which can be a clue about the structure of the stupa, related ruins of the 7th century period, and previous studies. As a result, this paper supposed and presented a model picture for the plane arrangement of pillars, structural methods for Dwibburi (뒤뿌리) of Haangjae (하앙재), Shimju (심주), and other key areas. The author hopes that this study contributes substantially to research on the wooden stupa of ancient Korea and to the establishment of construction and structural methods.

• Korean Journal of Heritage: History & Science
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• v.45 no.2
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• pp.72-99
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• 2012

Cheomseongdae, Korea's so-called "star-gazing tower" located in the former Silla Dynasty capital of Gyeongju, is generally believed to have maintained its original shape since its construction in 647. The stone structure was closely examined and measured by Gyeongju National Museum in 1962 and reexamined by Korean National Research Institute of Cultural Heritage in 2009. This research noted the following structural anomalies of Cheomseongdae. A corner of the top rectangle layer was broken diagonally in a form that can never be attributed to a natural cause. The four metal clamps under the top rectangle layer had been missing. Four grooves, with unknown usage, exist on the sides of the long rectangular stones projected out of the circular body at the second and third circular layers from the top. On the second circular layer from the top, there are three flat stones, less than a half as tall as their surrounding stones. The average height of the third circular layer from the top is only 23.5 cm, while the overall average of the entire 27 circular layers is 29.9cm. This research postulates that all these anomalies are due to prior destructions and improper restorations of the structure. The first destruction and restoration of Cheomsengdae was likely to have occurred before the mid 15th century. The damages might include a pavilion on the top of the structure and a stone with the name of the structure carved in. The Mongolian invasion in the 13th century was a most likely cause. After the restoration, the structure suffered at least another attack. The damages on the top layer and the missing iron clamps were due to the later attack. The grooves and flat stones were to house holding device that affixed certain objects to the outer surface of the circular body. The metal or stone objects might have faced upward at the four corners of Chemseongdae, bridging the gaps between the rectangle layers and circular layers. The current Chemseongdae lost at least the four affixed objects, four holding devices, and one flat stone.

• Journal of Life Science
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• v.29 no.9
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• pp.986-995
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• 2019

Intestinal microbiota play a key role in maintaining the host's health, and variety and richness of this microbiota is directly influenced and modulated by the host's diet. Kimchi is a fermented food rich in dietary fibers and lactic acid bacteria (LAB). To investigate the effect of fermented kimchi on the host's response and the composition of intestinal microbiota, 45 male Sprague-Dawley rats six weeks old were divided into three experimental groups that received either a basal diet (CON) or a basal diet supplemented with fermented kimchi (FK) or chitosan-added fermented kimchi (CFK) for four weeks. Body weights and feed intakes were measured weekly, and the intestinal contents were collected aseptically and were used for 16S rRNA gene profiling via pyrosequencing. As compared to the control, FK and CFK groups showed less body weight gain, feed efficiency, and blood triglyceride concentration. The diversity of intestinal microbiota was increased in both FK and CFK as compared to the control. At the phylum level, obesity-associated Firmicutes decreased, while leanness-associated Bacteroidetes increased. At the genus-level, the genera that consist of LAB, leanness-associated bacteria, and butyric acid-producing bacteria increased in FK and CFK as compared to the control. The overall results suggest that the consumption of fermented kimchi can reduce obesity and promote the host's health through mechanisms involving the modulation of intestinal microbiota.

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.390-400
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• 2019

In this study, strains isolated from soil samples collected from Busan, Changwon, and Jeju Island were examined to verify their abilities of phosphate solubilization and nitrogen fixation, production of indole-3-acetic acid (IAA), siderophore, and 1-aminocylopropane-1-carboxylyic acid (ACC) deaminase in order to select strains that promote plant growth and play a role in biocontrol of pests or pathogens. According to the results of this study, most of the isolated strains were found to have ability of phosphate solubilization, nitrogen fixation, IAA production, siderophore production, and production of ACC deaminase. These isolated strains might help plant growth by directly improving absorption of nutrients essential for phosphate solubilization and nitrogen fixation. In addition, they can promote plant growth and control resistance to plant diseases through extracellular enzyme activity and antifungal activity. In addition, most of the selected strains were found to survive in various environmental conditions such as temperature, salinity, and pH. Therefore, Pseudomonas plecoglossicida ANG14, Pseudarthrobacter equi ANG28, Beijerinckia fluminensis ANG34, and Acinetobacter calcoaceticus ANG35 were finally selected through a comparative advantage analysis to suggest their potential as novel biological agents. Further studies are necessary in order to prove their efficacy as novel biological agents through formulation and optimization of effective microorganisms, their preservation period, and crop cultivation tests.

• Korean Journal of Heritage: History & Science
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• v.47 no.4
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• pp.162-177
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• 2014

This article examined the burial type, wooden coffin and its historical significance of Iksan Ssangneung, the royal tombs of Baekje. Ssangneung was found to be the royal tombs of King Mu(武王) and queen through the burial type, wooden coffin, articles such as chinese jade ornaments and the deep relationship between King Mu and Iksan region. The reason of constructing Baekje royal tombs in Iksan region can be examined at many aspects. First, Iksan was a very important place for King Mu. Because Iksan was the basis of his political growth. Also, Iksan was a important geopolitical location in relation with Silla(新羅). For this reason, Iksan was developed in earnest. The construction of major facilities such as royal palace and temples in Iksan suggest the transfer of the capital from Sabi(泗?) or the preparation and partial execution at least. However, the transfer of the capital was not completed. It can be seen as the incomplete city. However, even after King Mu Iksan was maintained as the important region by King Euja(義慈王). It can be seen through the maintenance of great temples such as Jeseoksa(帝釋寺) and Mireuksa(彌勒寺) built at later period of King Mu and royal palace area, even though royal palace had turned into the temple. These inherited consciousness may have been embodied in the act of building royal tombs of King Mu and queen.

• Korean Journal of Heritage: History & Science
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• v.47 no.1
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• pp.46-65
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• 2014

This study, under the title of Daeungbojeon Hall of Bulyeongsa Temple, Uljin and the architectural technique of the architect, aimed to make a comprehensive speculation on Daeungbojeon Hall of Bulyeongsa Temple whose accurate construction year was confirmed in 1725. While existing studies dealt with it separately between construction and Buddhist art history this study attempted to sort out it in one perspective by means of comparison with compatible objects in the area. The results are as follows. 1st, by means of comparison of wall painting of Daeungbojeon Hall of Bulyeongsa Temple and other wall paintings of Gyeongsang-do Province, it was estimated to be created before and after 1725, the founding year of the building. 2nd, the stylobate of Daeungbojeon Hall is the only and unique case that Guibu was supported by the bottom. Such stylobate was estimated to be built in the early period of Goryeo stylobate of PostLintel Construction which was more simplified than that of the traditional unified Silla period considering specific techniques. Lastly, by means of comparison of the architectural technique of Daeungbojeon Hall of Bulyeongsa Temple with other temples in Gyeongsang-do Province, the characteristics of the building were found. In particular, the same architectural technique was confirmed by direct comparison of style with Yeongsanjeon Hall of Tongdosa Temple in 1714 which was constructed by the same architect.

• Korean Journal of Heritage: History & Science
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• v.48 no.3
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• pp.166-185
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• 2015

Recently, as an enonomic importance of the Eurasian Continent raises, the necessity of east-west connecting ancient transport roads research is increasing. Although domestic research of eastern-western international exchange in the Eurasia is not yet very active, the studies of history, archaeology, art history, folklore, costume history have been advanced steadily. An attention for the exchange through steppe route originated from the interest in Korean folk and Korean culture and the research range is extending to Xiungnu, Kurgan culture as a direct investigation on the remains of Mongolia and Kazakhstan has been achieved constantly. The art history has been leading the research of exchange which is based on desert and oasis. The field research of Iran, Uzbekistan, Azerbaijan, etc progressed in various routes, and the research on diverse topics including Silla's golden culture, transmission of glass, and the Buddhism is improving. Research on the maritime silk road is weaker, compared to other parts. Buddhist and Hindu temples of Southeast Asia attracted some interest to people, but the research should focus on the restoration of sea route and consideration of its meaning. Research of this part is expected to be more activative, as domestic researchers investigate Don Son culture and Sa Huynh culture of Vietnam by themselves. From now on, we should focus on topics that are not directly connected to Korean history, and Korean culture. Because it is also our duty to study and conserve the culture of entire human community.