• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.78-83
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• 2022

There are several subspecies of Bombyx mori, whose silk sericin variants differ. Silk sericin can induce bone morphogenic protein-2 (BMP-2) in macrophages, and silk sericin from different species may have different levels of BMP-2 induction ability. In this study, silk sericin from three B. mori subspecies (Baegokjam, Yeonnokjam, and Goldensilk) was prepared. They were administered to RAW264.7 cells and BMP-2 expression level was studied. Bone regeneration was evaluated using a rat calvarial defect model. BMP-2 expression level was the highest in the Baegokjam group. The bone volume in the Baegokjam group was significantly higher than that in the Yeonnokjam group (P = 0.003). In conclusion, sericin from Baegokjam showed higher levels of BMP-2 expression and bone regeneration than those from Yeonnokjam and Goldensilk.

• 한국잠사곤충학회지
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• 제43권1호
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• pp.41-48
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• 2001

The purification, dissolution and powdering of stained waste silk obtained from weaving and dyeing process were studied for the surface modification of textile fabric and plastic materials. The whiteness of stained waste silk could be improved through degumming and bleaching with sodium hydrosulfite. The water-soluble fibroin solution can be obtained by dissoving the degummed waste silk in a boiling solution of 50% calcium chloride for 60 minutes. The salts and heavy metals contained in fibroin solution were removed by electric dialysis, wool fiber filtration and gel filtration chromatography. The fibroin powder was prepared by using a fine grinder after the alkali treatment for weakening the silk fiber. The fine fibroin powder of particle size around 30 ㎛ was obtained with a ultra fine-mill, while it was finer below 10 ㎛ with a ball-mill. The dissolved or powdered silk was applied to the surface of fabric with addition of the binder (a urethane resin). The moisture content of polyester and nylon fabrics treated with the silk solution was improved due to hygroscopic property of silk. The fine fibroin powder mixed with the binder ws coated on the surface of synthetic film by use of the air pressed sprayer. It was revealed that the hygroscopicity as well as the softness of fibroin powder coated film was much improved. Therefore, it is thought that the fine silk fibroin powder is applicable as an coating agent for the surface modification of plastic and synthetic leather.

• Applied Biological Chemistry
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• 제12권
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• pp.75-81
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• 1969

세균성(細菌性) Sericinase를 생사(生絲)의 정련(精練)과 고치 해서(解舒)에 이용(利用)하고자 유용균(有用菌)을 분리(分離) 선별(選別)하였으며 이 균(菌)이 생성(生成)하는 Sericinase 의 여러 효소학적(酵素學的) 성질(性質)을 조사(調査)하여 생사정련(生絲精練)에 적용(適用)한 결과(結果)를 타정련법(他精練法)과 비교검토(比較檢討)하였다. 1. 생사정련작용(生絲精練作用)과 견제교작용(繭除膠作用)이 강(强)하며 견(絹) Fibroin에 대(對)해서는 전연(全然) 작용(作用)하지 아니하는 Sericinase를 생성(生成)하는 세균(細菌) 일주(一走)를 분리(分離) 선별(選別)하였다. 2. 본(本)균주(菌株)에 의(依)해 생성(生成)된 Sericinasedml 최적작용(最適作用) pH는 7.5에 위치(位置)하였으며 최적작용(最適作用) 온도는 $50^{\circ}C$였다. 이 온도(溫度)에서는 견섬유(絹纖維)의 modification 등(等)은 일어나지 않을 것으로 믿어지며 3. 본(本) 효소(酵素)는 free incubation에 있어서는 $Ca^{++}$이 효소실활(酵素失活)을 촉진(促進)하며 기질(基質)에 사용(使用)할 때는 Ca-oxalate 일 경우(境遇) 효소작용(酵素作用)을 촉진(促進)하므로 작잠등(?蠶等)으 해서(解舒)에 유용(有用)할 것으로 믿어지며 4. 본(本) 효소(酵素)는 견사(絹絲) 중(中)의 Sericin을 거의 완전(完全)하게 분해(分解)하는 것으로 믿어지며 5. 본(本) 효소(酵素)를 사용(使用)하여 졍련(精練)한 견(絹)을 조사(調査)한 바그 생사표면(生絲表面)의 상태(狀態), Fibroin의 손상(損傷), 촉감(觸感), 정련효과(精練效果) 등(等)이 타(他) 정련법(精練法) 즉(卽) 석감법, Alkali법(法), Alkali 석(石) 감법보다 우수(優秀)하였으며, 고급정련볍(高級精練法)에만 이용(利用)하는 papain이나 pancreatin과 같은 효과(效果)를 얻을 수 있으리라 기대(期待)된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제19권2호
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• pp.265-268
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• 2009

Wild silkmoths can be utilised sustainably in the production of silk as an income for resource-poor rural communities. However, attack by parasitoids and predators affect the quality of cocoons and quantity of raw silk produced. A laboratory experiment was undertaken to quantify the effect of parasitoids' (dipteran and hymenopteran) and predators' (ants) exit and ingress holes, respectively, on silk production. The mean number of shells required to produce fifty grams of raw silk was highest with cocoons parasitised by a dipteran and lowest with unattacked cocoons (but with moths already emerged). Degumming loss was highest in parasitised and lowest in unttacked cocoons, but both were not different from cocoons predated by ants. Shell weight was highest in unattacked cocoons, followed by hymenopteran-parasitised and predated cocoons, with the dipteran parasitized ones being the least. Single cocoon weight was greater in hymenopteran-parasitised and predated cocoons than the dipteran-parasitised and unattacked cocoons. Shell ratio or raw silk, floss and yarn weights were higher in unattacked than parasitised and predated cocoons. The total loss in raw silk attributable to attack by parasitoids and predators ranged between 17.4~31.2%. The results offer baseline information for assessment of economic losses in wild silk farming due to parasitoids and predators in the field.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2007년도 추계학술발표회
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• pp.45-46
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• 2007

• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.203-208
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• 2013

Sericin is usually abandoned after the degumming process. However, it could be a valuable bioresource if an economically efficient recovery process could be set up. In this study, sericin was recovered directly from the degummed waste solution by adding calcium chloride, which induced the precipitation of the surfactant, sodium oleate, by charge interaction. The recovery yield was maximum when 10% of calcium chloride was added. Further increase in the calcium chloride concentration induced the precipitation of sericin. The recovered sericin had a molecular weight distribution similar to that of the hot-water-extracted sericin; but some highmolecular- weight sericin could not be recovered. The secondary structure and amino acid composition of the recovered sericin were similar to those of conventional hot-water-extracted sericin. We expect that sericin recovered from the degummed waste solution could be an alternative to the hot-water-extracted sericin, which is widely used in various applications.

• 한국잠사곤충학회지
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• 제40권2호
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• pp.150-157
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• 1998

This study was carried out to define the effects of anti-juvenile hormone analogue(AJH) treatment at different silkworm larval stages on some silk filament properties. It was revealed that the treatments at the 1st and 2nd days of the 3rd instar as well as the 1st day of the 4th instar resulted to trimolters induction without lethal effect. The trimolters induced by treatment at the 1st day of the 4th instar showed the shortest larval duration and the lowest single cocoon weight, cocoon shell ratio, cocoon size, single filament length and denier of filament compared with those of the control, while the trimolters induced by the 3rd-instar treatment showed intermediate values. All treatments showed no significant differences from the aspect fo degumming ratio, elongation rate and crystallinity index of fibroin. However the silk filament obtained from the treatment at the 1st day of the 4th instar showed the highest tenacity which was revealed to be related to the better uniformity of filament by the means of microscopic photographing. FT-IR spectroscopy of the silk fibroin showed a sharp and strong peak at the position of 794.72 cm-1 in all AJH-treated samples regardless of trimolters induction, which was absent in control.

• 한국의류학회지
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• 제34권5호
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• pp.754-764
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• 2010

Most silk fabrics are produced only after the degumming process to make the best use of the properties and have restricted silk processing that do not hinder their performance. However, considering the highly increased preference for natural fibers and the shortage of raw silk, high-quality upgraded silk product functions are required by the development of a processing technology and a good design. This study analyzes the changes with the samples by the functional finish such as softening finishing, wash and wear, tannin weighting by measuring the objective hand of scoured silk and three finished ones using KES-FB. As a result, the change of objective hand of finished silk fabrics that improves functionality was analyzed and compared. The increase of KOSHI after the finish became stiffer show that the silk fabric samples are appropriate for summertime clothes with the retention of a certain clothing climate for the body. The stiffness of finished fabrics for the normal had a closer relationship with the density of fabrics than the type of finishing. The samples (after the softening finishes) maintain better elasticity according to the properties of the softener and the finishing agent. Although the specimens of this study were thin fabrics, their elasticity against compression increased after the softening finishes and became softer than degummed silk. The surface properties of georgette were changed by all types of finishing.

• 한국잠사곤충학회지
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• 제29권2호
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• pp.67-72
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• 1987

견직물의 효소정련에 있어서 조련정도가 효소정련한 견직물의 특성에 미치는 영향을 구명하기 위하여 조련정도가 다른 효소정련견포에 대하여 KES-F System을 적용하여 역학적 특성 즉 인장, 굽힘, 약단, 압축 및 표면특성을 측정하고 태값을 산출하여 비교한 결과는 아래와 같다. 1. 능하부다이 견직물의 경우 조련연감율이 증가와 함께 포의 stiffness는 감소되고 smoothness는 향상되었으나 종합 태값인 soft feeling은 조련 20%에서 가장 우수하였다. 2. 크\ulcorner데신 견직물의 경우 anti-drape stiffness는 조련정도가 커지는데 따라서 증가되었으나 기타 특성과 종합 태값인 Flexibility with soft feeling은 조련정도와 상관이 인정되지 않았다.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2003년도 봄 학술발표회 논문집
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• pp.275-278
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• 2003

실크 세리신은 누에(Bombyx mori)로부터 얻어지는 천연 거대분자 단백질로서, 누에고치실의 20~30%를 차지하고 있다. 세리신은 외부로의 자극으로부터 피브로인을 보호하는 역할을 하지만, 견사나 견직물을 얻기 위해서는 정련 공정에서 반드시 제거하게 된다. 현재 생산 현장에서는 비누/알칼리 정련법으로 세리신을 제거하고 있는데, 정련 폐액중에 포함되어 있는 세리신 단백질에서 분해된 각종 아미노산과 비누 및 강알칼리 약제로 인해 환경오염의 주된 원인이 되고 있다. (중략)