• KSBB Journal
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• v.17 no.4
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• pp.370-375
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• 2002

Isolation of a gene and determination of its expression pattern are essential in understanding its function. Among the genes localized in 12ql3, stSG3435 EST was chosen to study its expression pattern. The full-length CDNA was cloned by screening of human brain CDNA library and its sequence was determined by serial deletion followed by automated sequencing of the clones with overlapping fragments. The sequence analysis revealed that stSG 3435 CDNA displayed 100% identity to human MYGI and 86% identity to mouse melanocyte proliferation gene-1 (Gamm 1) originally identified from melanocyte, suggesting that MYGI determined by Northern blot analysis revealed the strongest expression in testes with ubiquitous expression in all the tissues tested. In order to investigate the cellular localization of its protein product, the green fluorescence protein gene was fused into the full-length coding sequence of MYGI, Transfection of the fusion construct followed by confocal microscopy resulted in the green fluorescence signal as a punctate state in cytoplasm indication that MYGI was localized in one of the cellular organelles.

• Investigative Magnetic Resonance Imaging
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• v.20 no.2
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• pp.81-87
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• 2016

Purpose: To analyze the feasibility of three-dimensional (3D) diffusion-weighted (DW) PSIF (reversed FISP [fast imaging with steady-state free precession]) sequence in order to evaluate peripheral nerves in the elbow. Materials and Methods: Ten normal, asymptomatic volunteers were enrolled (6 men, 4 women, mean age 27.9 years). The following sequences of magnetic resonance images (MRI) of the elbow were obtained using a 3.0-T machine: 3D DW PSIF, 3D T2 SPACE (sampling perfection with application optimized contrasts using different flip angle evolution) with SPAIR (spectral adiabatic inversion recovery) and 2D T2 TSE (turbo spin echo) with modified Dixon (m-Dixon) sequence. Two observers used a 5-point grading system to analyze the image quality of the ulnar, median, and radial nerves. The signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of each nerve were measured. We compared 3D DW PSIF images with other sequences using the Wilcoxon-signed rank test and Friedman test. Inter-observer agreement was measured using intraclass correlation coefficient (ICC) analysis. Results: The mean 5-point scores of radial, median, and ulnar nerves in 3D DW PSIF (3.9/4.2/4.5, respectively) were higher than those in 3D T2 SPACE SPAIR (1.9/2.8/2.8) and 2D T2 TSE m-Dixon (1.7/2.8/2.9) sequences (P < 0.05). The mean SNR in 3D DW PSIF was lower than 3D T2 SPACE SPAIR, but there was no difference between 3D DW PSIF and 2D T2 TSE m-Dixon in all of the three nerves. The mean CNR in 3D DW PSIF was lower than 3D T2 SPACE SPAIR and 2D T2 TSE m-Dixon in the median and ulnar nerves, but no difference among the three sequences in the radial nerve. Conclusion: The three-dimensional DW PSIF sequence may be feasible to evaluate the peripheral nerves around the elbow in MR imaging. However, further optimization of the image quality (SNR, CNR) is required.

• Korean Journal of Radiology
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• v.22 no.9
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• pp.1525-1536
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• 2021

Objective: To investigate the feasibility of cine three-dimensional (3D) balanced steady-state free precession (b-SSFP) imaging combined with a non-local means (NLM) algorithm for image denoising in evaluating cardiac function in children with repaired tetralogy of Fallot (rTOF). Materials and Methods: Thirty-five patients with rTOF (mean age, 12 years; range, 7-18 years) were enrolled to undergo cardiac cine image acquisition, including two-dimensional (2D) b-SSFP, 3D b-SSFP, and 3D b-SSFP combined with NLM. End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and ejection fraction (EF) of the two ventricles were measured and indexed by body surface index. Acquisition time and image quality were recorded and compared among the three imaging sequences. Results: 3D b-SSFP with denoising vs. 2D b-SSFP had high correlation coefficients for EDV, ESV, SV, and EF of the left (0.959-0.991; p < 0.001) as well as right (0.755-0.965; p < 0.001) ventricular metrics. The image acquisition time ± standard deviation (SD) was 25.1 ± 2.4 seconds for 3D b-SSFP compared with 277.6 ± 0.7 seconds for 2D b-SSFP, indicating a significantly shorter time with the 3D than the 2D sequence (p < 0.001). Image quality score was better with 3D b-SSFP combined with denoising than with 3D b-SSFP (mean ± SD, 3.8 ± 0.6 vs. 3.5 ± 0.6; p = 0.005). Signal-to-noise ratios for blood and myocardium as well as contrast between blood and myocardium were higher for 3D b-SSFP combined with denoising than for 3D b-SSFP (p < 0.05 for all but septal myocardium). Conclusion: The 3D b-SSFP sequence can significantly reduce acquisition time compared to the 2D b-SSFP sequence for cine imaging in the evaluation of ventricular function in children with rTOF, and its quality can be further improved by combining it with an NLM denoising method.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.459-464
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• 1997

The human liver cDNA clone UDPGTh2, encoding a liver UDP-glucuronosyltransferase (UDPGT) was isolated from a .gamma. gt 11 cDNA library by hybridization to mouse transferase cDNA clone, UDPGTm1. UDPGTh2 encoded a 529 amino acid protein with an amino terminus membrane-insertion signal peptide and a carboxyl terminus membrane-spanning region. There were three potential asparagine-linked glycosylation sites at residues 67, 68, and 315. In order to obtain UDPGTh2 protein encoded from cloned human liver UDP-glucuronosyltransferase cDNA, the clone was inserted into the pSVL vector (pUDPGTh2) and expressed in COS 1 cells. The presence of a transferase with Mr-52,000 in transfected cells cultured in the presence of $[^{35}S]$ methionine was shown by immunocomplexed products with goat antimouse transferase IgG and protein A-Sepharose and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The expressed UDPGT was a glycoprotein as indicated by electrophoretic mobility shift in Mr-3,000-4,000 when expressed in the presence of tunicamycin. The extent of glycosylation was difficult to assess, although one could assume that glycosyl structures incorporated at the level of endoplasmic reticulum were always the core oligosaccharides. Thus, it is likely that at least two moieties inserted can account for the shift of Mr-3,000-4,000. This study demonstrates the cDNA and deduced amino acid sequence of human liver UDP-glucuronosyltransferase cDNA, UDPGTh2.

• IEIE Transactions on Smart Processing and Computing
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• v.1 no.3
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• pp.152-160
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• 2012

This paper proposes a frame-level rate control algorithm for low delay video applications to reduce the fluctuations in the bitrate. The proposed algorithm minimizes the bitrate fluctuations in two ways with minimal coding loss. First, the proposed rate control applies R-Q model to all frames including the first frame of every group of pictures (GOP) except for the first one of a sequence. Conventional rate control algorithms do not use any R-Q models for the first frame of each GOP and do not estimate the generated-bit. An unexpected output rate result from the first frame affects the remainder of the pictures in the rate control. Second, a rate-distortion (R-D) cost is calculated regardless of the hierarchical coding structure for low bitrate fluctuations because the hierarchical coding structure controls the output bitrate in rate distortion optimization (RDO) process. The experimental results show that the average variance of per-frame bits with the proposed algorithm can reduce by approximately 33.8% with a delta peak signal-to-noise ratio (PSNR) degradation of 1.4dB for a "low-delay B" coding structure and by approximately 35.7% with a delta-PSNR degradation of 1.3dB for a "low-delay P" coding structure, compared to HM 8.0 rate control.

• Toxicological Research
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• v.19 no.3
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• pp.161-172
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• 2003

Explanted mammalian cells perform a limited number of cell division in vitro and than are arrested in a state known as replicative senescence. Such cells are irreversibly blocked, mostly in the G1 phase of cell cycle, and are no longer sensitive to growth factor stimulation. Thus replicative senescence is defined as a permanent and irreversible loss of replicative potential of cells. For this characteristic, replicative senescence seems to evolve to protect mammalian organism from cancer. However, senescence also contributes to aging. It seems to decrease with age of the cell donor and, as a form of cell senescence, is thought to underlie the aging process. Extensive evidence supports the idea that progressive telomere loss contributes to the phenomenon of cell senescence. Telomeres are repetitive structures of the sequence (TTAGGG)n at the ends of linear chromosomes. It has been shown that the average length of telomere repeats in human somatic cells decreases by 30∼200 bp with each cell division. It is generally believed that when telomeres reach a critical length, a signal is activated to initiate the senescent program. This has given rise to the hypothesis that telomeres act as mitotic clocks to regulate lifespan. One proposes that cumulative oxidative stress, mainly reactive oxygen species generated from mitochondria, may mainly cause telomere shortening, accelerating aging. Here, the biological importance and mechanism of replicative senescence were briefly reviewed. Also it was summarized that how oxidative stress affects replicative senescence and telomere shortening.

• Proceedings of the KIEE Conference
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• 2008.04a
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• pp.113-114
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• 2008

본 논문에서는 HD 비디오 영상 처리를 효과적으로 수행할 수 있는 다중 DSP 아키텍쳐를 제안하고 프로토타입 보드를 설계 제작하였다. 또한, 구현된 보드를 이용하여 비디오 영상 내 물체(얼굴)의 실시간 추석시스템을 구현하였다. 물체 추적 기법인 PF(Particle Filtering) 기법은 배경 클러터가 존재하는 환경에서도 강인하게 물체를 추적할 수 있지만 많은 수의 샘플을 사용하는 경우 필요한 계산량이 많아져 실시간 구현이 매우 어렵다는 문제점을 가지고 있다. 본 논문에서는 이러한 경우에도 실시간 추적이 가능하도록 병렬화된 PF 추적 방법을 제안하고 제작된 보드 상에 구현하였다. 구현된 병렬 처리 추적에서는 150개의 PF 샘플들을 5개의 슬레이브 DSP로 분산하여 컬러 유사도 기탄의 관측 확률을 계산하고 그 결과를 마스터 DSP에서 종합하여 추적의 정확도를 높이고자 하였다. 실험에는 $720{\times}480$ 픽셀 영상이 사용되었으며, 실험 결과 배경 클러터가 존재하는 경우에도 충분한 PF 샘플 수의 사용에 따라 대상 물체를 강인하게 추적하는 우수한 성능을 확인할 수 있었다.

• Journal of Korea Multimedia Society
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• v.16 no.2
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• pp.131-137
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• 2013

Zoom motion estimation of video sequence is very complicated for implementation. In this paper, we propose a method to implement the zoom motion estimation using together the depth camera and color camera. Depth camera obtains the distance information between current block and reference block, then zoom ratio between both blocks is calculated from this distance information. As the reference block is appropriately zoomed by the zoom ratio, the motion estimated difference signal can be reduced. Therefore, the proposed method is possible to increase the accuracy of motion estimation with keeping zoom motion estimation complexity not greater. Simulation was to measure the motion estimation accuracy of the proposed method, we can see the motion estimation error was decreased significantly compared to conventional block matching method.

• Bulletin of the Korean Chemical Society
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• v.34 no.7
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• pp.2063-2066
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• 2013

While many eukaryotic and some prokaryotic proteins show a phosphorylation-dependent mobility shift (PDMS) on SDS-PAGE, the molecular mechanism for this phenomenon had not been elucidated. We have recently shown that the distribution of negatively charged amino acids around the phosphorylation site is important for the PDMS of some proteins. Here, we show that replacement of the phosphorylation site with a negatively charged amino acid results in a similar degree of the mobility shift of a protein as phosphorylation, indicating that the PDMS is due to the introduction of a negative charge by phosphorylation. Compared with a protein showing no shift, one showing a retarded mobility on SDS-PAGE had a decreased capacity for SDS binding. The elucidation of the consensus sequence (${\Theta}X_{1-3}{\Theta}X_{1-3}{\Theta}$, where ${\Theta}$ corresponds to an acidic function) for a PDMS suggests a general strategy for mutagenizing a phosphorylatable protein resulting in a PDMS.

• Journal of international Conference on Electrical Machines and Systems
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• v.1 no.4
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• pp.457-463
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• 2012

Cascaded H-bridge multilevel inverters shows a useful circuit configuration to increase the number of output voltage levels to obtain high quality output voltage. By applying the concept of the power of three to dc voltage sources, it can increase the number of output voltage levels effectively. To realize this concept, two approaches may be considered. One is to use independent dc voltage sources pre-scaled in the power of three, and the other is to use instantaneous dc voltage sources generated from a cascaded transformer, which has the secondary turn-ratios scaled in the power of three in sequence. A common feature in both approaches is to use the concept of the power of three for dc voltage sources, and a point of difference is whether it adopts a low frequency transformer or not, and where the transformer is located. According to the difference, application areas are limited and show different characteristics on THD of output voltages. We compare and analyze both approaches for their circuit configurations, voltage level generating method, THD characteristics of output voltage, efficiency, application areas, limitations, and other characteristics by experiments using 500 [W] prototypes when they generate a 27-level output voltage.