• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.205-208
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• 2010

We introduce a novel feature extraction scheme for online handwritten digit based on utilizing Zernike moment and angulation feature. The time sequential signal from mouse movement on the writing pad is described as a sequence of consecutive points on the x-y plane. So, we can create data-set which are successive and time-sequential pixel position data by preprocessing. Data preprocessed is used for Zernike moment and angulation feature extraction. this feature is scale-, translation-, and rotation-invariant. The extracted specific feature is fed to a BP(backpropagation) neural network, which in turn classifies it as one of the nine digits. In this paper, proposed method not noly show high recognition rate but also need less learning data for 200 handwritten digit data.

• The KIPS Transactions:PartB
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• v.13B no.2 s.105
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• pp.97-104
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• 2006

There is no method to control for the child efficiently when disease happens who cannot be able to express his symptoms. Therefore, doctor's diagnosis depends on inquiring from child's patients, that leads to wrong diagnosis result. For this, in this paper, we would like to develop child ocular inspection, auscultation diagnosis instruments, using Oriental medicine principle that living body signal of five organs and six hallow organs which reflects patients face and voice We would like to get more accurate diagnosis result for child's symptoms from doctor's intuition on the basis of diagnostic sight visualization, objectification, quantization itself. This paper develops color revision, YCbCr application, and face color selection and five sensory organs and nose or apex extraction method etc, in child ocular inspection by first work achievement sequence among the whole development systems. Also, in occasion of child auscultation, crying characteristics of colics through pitch, intensity and formant analysis is numerized and objectifies doctor's intuition through this. Finally, experiments are performed to verify the effectiveness of the proposed methods.

• Journal of radiological science and technology
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• v.20 no.2
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• pp.73-78
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• 1997

The goal of this paper is that we know the usefulness of echo-planar imaging(EPI) for discriminate between hepatocellular carcinoma(HCC) and hemangioma. We get a time signal intensity curve for liver diseases from the dynamic contrast enhancement images and compared and analyze both the contrast ratio(CR) and the contrast to noise ratio(CNR) using echo planar imaging. The obtained results are follows : 1. Hepatocellular carcinoma was shown the best contrast after about 20 seconds when Is the earlist time in the main artery, and then reduced. The center where is disease was shown the characteristic that the best contrast is appeared after about 35-45 seconds and then slowly reduced. Liver parenchyma was shown the best contrast and reduced after 60 seconds. 2. The peripheral nodular of hemangioma was shown the better contrast soon. On the other hend, the contrast of center where is disease started to increase after 60 seconds and was equal to that of liver parenchyma. Increasing of the contrast continued after. 3. Turbo SE technic was used, the average of CR for hepatocellular carcinoma was $36.7{\pm}1.2$ and the average of CNR was $2.4{\pm}3.2$, while the average of CNR for hemangioma was $54.9{\pm}1.0$ and the average of CNR was $9.7{\pm}1.3$. 4. EPI technic was used, the average of CR for hepatocellular carcinoma was $47.8{\pm}1.2$ and the average of CNR was $3.4{\pm}2.1$, while the average of CNR for hemangioma was $75.7{\pm}2.2$ and the average of CNR was $9.5{\pm}1.1$. According to above we can find that hemangioma is more bright than hepatocellular carcinoma and the difference of brightness between hepatocellular carcinoma and hemangioma is useful sequence.

• Molecules and Cells
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• v.28 no.5
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• pp.463-472
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• 2009

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of development in rice plants.

• Journal of Genetic Medicine
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• v.17 no.1
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• pp.39-42
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• 2020

The Senior-Loken syndrome was first described in 1961 as an oculo-renal disease consisting of familial juvenile nephronophthisis and Leber congenital amaurosis. It is a rare autosomal recessive disorder with a prevalence of 1:1,000,000 caused by mutations in nine genes (NPHP 1-8 and NPHP 10). Ocular manifestations (e.g., photophobia, nystagmus, and extreme hyperopia) occur within the first few years of life while renal manifestations (e.g., formation of multiple cysts impairing kidney function and end-stage renal disease) appear in late childhood to adolescence. Here, we report a case of a Filipino male presenting with rotatory nystagmus and progressive deterioration of vision since childhood. He had congenital amaurosis and juvenile nephronophthisis that progressed to end stage renal disease by age 19. All laboratory and imaging findings were consistent with chronic kidney disease. Molecular genetic testing of ciliopathy-related genes was performed revealing a homozygous mutation in exon 11 of the IQCB1/NPHP5 gene, c.1090C>T (p.Arg364^⁎). This sequence change created a premature translational stop signal resulting in a truncated protein product, nephrocystin-5 and its consequent loss of function. His symptoms eventually improved with initiation dialysis. The prognosis of Senior-Loken syndrome remains dismal and a high index of suspicion, early diagnosis and timely intervention of renal complications are warranted.

• Journal of the Korean Magnetic Resonance Society
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• v.20 no.3
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• pp.95-101
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• 2016

Apolipoprotein B-100 (Apo-B100) is a major component of low density lipoprotein (LDL). Apo B-100 protein has 4,536 amino acid sequence and these amino acids are classified into peptide groups A to G with subsequent 20 amino acids (P1-P302). The peptide groups were act as immunoglobulin (Ig) antigens which oxidized via malondialdehyde (MDA). The mimetic peptide P1 (EEEMLENVSLVCPKDAT RFK) out of D-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Circular Dichroism (CD) spectra were measured for peptide secondary structure in the range of 190-250 nm. Experimental results show that P1 exhibit partial of ${\beta}-sheet$ and random coil structure. Homonuclear (COSY, TOCSY, NOESY) 2D-NMR experiments were carried out for NMR signal assignments and structure determination for P1. On the basis of these completely assigned NMR spectra and distance data, distance geometry (DG) and Molecular dynamics (MD) were carried out to determine the structures of P1. The proposed structure was selected by comparisons between experimental NOE spectra and back calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P1 obtained upon superposition of all atoms was in the range $0.33{\AA}$. The solution state P1 has mixed structure of ${\beta}-sheet$ (Glu[1] to Cys[12]) and random coil (Pro[13] to Lys[20]). These NMR results are well consistent with secondary structure from experimental results of circular dichroism. Structural studies based on NMR may contribute to the studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.9
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• pp.1734-1741
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• 2007

In this paper, the Hybrid SC/MRC-2/4 method in which bit synchronization and phase synchronization were not required was applied to the orthogonal MC DS-CDMA system in which each normalized subcarrier interval and processing gain had the same value, respectively, and the direct sequence spread code of each subcarrier was orthogonal. In the broadband wireless system in which multi-carrier transmission was used, a Doppler frequency shift occurred, which was caused by the difference between the highest subcarrier frequency md the lowest subcarrier frequency. In order to complement phase error caused by the shift, the orthogonal MC DS-CDMA system was analyzed so that the receiving signal could be perfectly synchronized by adjusting the PLL gain suitable for the entire system. As a result of simulations, as the PLL gain was increased, the change in the intervals was close to the case of perfect synchronization however, it became less when the PLL gain reached more than a certain value. Therefore, by selecting a proper PLL gain suitable for the system the orthogonal MC DS-CDMA can be designed in which the Hybrid SC/MRC method is applied.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.625-632
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• 2019

The unified saccharification and fermentation (USF) system was developed for direct production of ethanol from agarose. This system contains an enzymatic saccharification process that uses three types of agarases and a fermentation process by recombinant yeast. The $pGMF{\alpha}-HGN$ plasmid harboring AGAH71 and AGAG1 genes encoding ${\beta}-agarase$ and the NABH558 gene encoding neoagarobiose hydrolase was constructed and transformed into the Saccharomyces cerevisiae 2805 strain. Three secretory agarases were produced by introducing an S. cerevisiae signal sequence, and they efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexose. To directly produce ethanol from agarose, the S. cerevisiae $2805/pGMF{\alpha}-HGN$ strain was cultivated into YP-containing agarose medium at $40^{\circ}C$ for 48 h (for saccharification) and then $30^{\circ}C$ for 72 h (for fermentation). During the united cultivation process for 120 h, a maximum of 1.97 g/l ethanol from 10 g/l agarose was produced. This is the first report on a single process containing enzymatic saccharification and fermentation for direct production of ethanol without chemical liquefaction (pretreatment) of agarose.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.4
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• pp.1765-1794
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• 2019

Genetic Programming (GP) is an intelligence technique whereby computer programs are encoded as a set of genes which are evolved utilizing a Genetic Algorithm (GA). In other words, the GP employs novel optimization techniques to modify computer programs; imitating the way humans develop programs by progressively re-writing them for solving problems automatically. Trial programs are frequently altered in the search for obtaining superior solutions due to the base is GA. These are evolutionary search techniques inspired by biological evolution such as mutation, reproduction, natural selection, recombination, and survival of the fittest. The power of GAs is being represented by an advancing range of applications; vector processing, quantum computing, VLSI circuit layout, and so on. But one of the most significant uses of GAs is the automatic generation of programs. Technically, the GP solves problems automatically without having to tell the computer specifically how to process it. To meet this requirement, the GP utilizes GAs to a "population" of trial programs, traditionally encoded in memory as tree-structures. Trial programs are estimated using a "fitness function" and the suited solutions picked for re-evaluation and modification such that this sequence is replicated until a "correct" program is generated. GP has represented its power by modifying a simple program for categorizing news stories, executing optical character recognition, medical signal filters, and for target identification, etc. This paper reviews existing literature regarding the GPs and their applications in different scientific fields and aims to provide an easy understanding of various types of GPs for beginners.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.73-79
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• 2019

A novel esterase gene (est7S) was cloned from an enriched metagenomic library derived from an oil-spill area. The gene encoded a protein of 505 amino acids, and the molecular mass of the Est7S was estimated to be 54,512 Da with no signal peptide. Est7S showed the highest identity of 40% to an esterase from a sludge metagenome compared to the characterized enzymes with their properties, although it showed 99% identity to a carboxylesterase in the genome sequence of Alcanivorax borkumensis SK2. Est7S had catalytic triad residues, Ser183, Glu312, and His420, and the GESAG motif in most family VII lipolytic enzymes. Est7S was purified from the crude extract of clone SM7 using Sephacryl S-200 HR and HiTrap Q column chromatographies. The purified Est7S was optimally active at $50^{\circ}C$ and pH 10.0. Est7S showed a high specific activity of 366.7 U/mg protein. It preferred short length esters, particularly p-nitrophenyl acetate, efficiently hydrolyzed R- and S-enantiomers of methyl-3-hydroxy-2-methylpropionate, and glyceryl tributyrate. These properties of Est7S may provide potential merits in biotechnological applications such as detergent and paper processing under alkaline conditions.