• BMB Reports
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• v.39 no.6
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• pp.717-721
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• 2006

In vitro analyses of type I signal peptidase activities require protein precursors as substrates. Usually, these pre-proteins are expressed in vitro and cleavage of the signal sequence is followed by SDS polyacrylamide gel electrophoresis coupled with autoradiography. Radioactive amino acids have to be incorporated in the expressed protein, since the amount of the in vitro expressed protein is usually very low and processing of the signal peptide cannot be followed by SDS polyacrylamide gel electrophoresis alone. Here we describe a rapid and simple method to express large amounts of a protein precursor in E. coli. We have analyzed the effect of ionophors as well as of azide on the accumulation of expressed protein precursors. Azide blocks the function of SecA and the ionophors dissipate the electrochemical gradient across the cytoplasmic membrane of E. coli. Addition of azide ions resulted in the formation of inclusion bodies, highly enriched with pre-apo-plastocyanine. Plastocyanine is a soluble copper protein, which can be found in the periplasmic space of cyanobacteria as well as in the thylakoid lumen of cyanobacteria and chloroplasts, and the pre-protein contains a cleavable signal sequence at its N-terminus. After purification of cyanobacterial pre-apo-plastocyanine, its signal sequence can be cleaved off by the E. coli signal peptidase, and protein processing was followed on Coomassie stained SDS polyacrylamide gels. We are optimistic that the presented method can be further developed and applied.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1998.06a
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• pp.423-425
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• 1998

An optical system which can autonomously form and display an impression of a picture made up by many figures has been developed. This system consists of optical fuzzy-neurons which calculate the correlation between the input picture and the reference image by incoherent optics. The calculated signal is applied to an amplifier whereby the output signal increases, then decreases according to increase of the input signal . These outputs are synthesized, and are used for changing the position where the system gaze on a part of the input picture by light beam. In this system, the light intensity used for gazing changes chaotically, The attractor drawn from the change of light intensity corresponds to the impression of the picture. This paper shows the results that are calculated by the numerical simulation. The system has been simulated to express the impression for a picture formed by 4figures.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.11
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• pp.943-950
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• 2008

A novel nondestructive testing (NDT) system, which is able to detect a crack with high speed and high spatial resolution, is urgently required for inspecting small cracks on express train wheels. This paper proposes a high speed scan type magnetic camera, which uses the multiple amplifying circuits and the crack indicating pulse output system. The linearly integrated Hall sensors are arrayed in parallel, and the Hall voltages from each sensor in the scanning direction are obtained and amplified. High-speed NDT can be achieved by using the exclusive analog-digital converter and micro-processor because the ${\partial}\;V_H/\;{\partial}$ x value, which provides the most important crack information, can be obtained by buffering and calculating. The effectiveness of the novel method was verified by examine using cracks on the wheel specimen model.

• Proceedings of the Korean Biophysical Society Conference
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• 2001.06a
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• pp.23-23
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• 2001

To evaluate the contribution of cAMP/PKA signal pathway in short-term facilitation, we overexpressed Ap oal receptor in sensory neurons that do not normally express this receptor. We have previously shown that activation of this receptor in sensory cells, by a brief treatment with octopamine (OA), produced short-term facilitation such as membrane depolarization, increase in membrane excitability, spike broadening, and enhanced neurotransmitter release in non-depressed synapse.(omitted)

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.6
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• pp.1025-1031
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• 2001

In this paper, we derive the outage probability of cellular mobile system with STTB(Space Time Transmitter Diversity) scheme where the received radio signals and interferers experience Rayleigh fading and AWGN. The new probability density function of L independent identically distributed interferers is derived using Laplace transforms. We express the probability of outage as a function of the average-signal to average-interference power ratio and the signal to noise ratio. In addition, the frequency reuse distance which is one of the key parameters in the design of cellular systems is analyzed.

• Proceedings of the KSR Conference
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• 2005.05a
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• pp.831-835
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• 2005

In a signal control of the railway system, the first objective is to guarantee a safety of the train operation, and the second is to increase a frequency(a capability of the transportation) of the train operation. In order to express the capability of the transportation a terminology what is called a railroad line capacity is employed. The railroad line capacity means a maximum frequency of the train operation in a possible schedule of the one way operation for one day. During the last several years an improvement in the facilities of the train operation (railroad line extension, improvement in the stations, improvement in the signal facilities) has been achieved to increase the railroad line capacity. In this paper the authors deal with the case analysis which try to shorten headway and which has an impartible relation with increasing of the railroad capacity

• Journal of Biomedical Engineering Research
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• v.28 no.4
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• pp.494-502
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• 2007

Ubiquitous location based services (u-LBS) will be interested to an important services. They can easily recognize object position at anytime, anywhere. At present, many researchers are making a study of the position recognition and tracking. This paper consists of postion recognition and user identification system. The position recognition is based on location under services (LBS) using a signal strength map, a database is previously made use of empirical measured received signal strength indicator (RSSI). The user identification system automatically controls instruments which is located in home. Moreover users are able to measures body signal freely. We implemented the multi-hop routing method using the Star-Mesh networks. Also, we use the sensor devices which are satisfied with the IEEE 802.15.4 specification. The used devices are the Nano-24 modules in Octacomm Co. Ltd. A RSSI is very important factor in position recognition analysis. It makes use of the way that decides position recognition and user identification in narrow indoor space. In experiments, we can analyze properties of the RSSI, draw the parameter about position recognition. The experimental result is that RSSI value is attenuated according to increasing distances. It also derives property of the radio frequency (RF) signal. Moreover, we express the monitoring program using the Microsoft C#. Finally, the proposed methods are expected to protect a sudden death and an accident in home.

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.3
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• pp.143-148
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• 2007

The many studies have been proceeding to express accurately the feature of a sudden signal and a uncertain system in the image processing field. It is well know that Fourier Transform is widely used for frequency analysis of any signal. However, The frequency transform domain is not used for expressing the sudden signal change and non-stationary signal at the time-axis by this method. This paper describes of image analysis by discrete wavelet transform. Wavelet modulus maxima on transformed plane gives the Lipschitz exponent expression, which is useful to examine the characteristics of signal or the edge of an image. It is possible to reconstruct the original image only using the few maxima points. The fractal analysis is applied as an examples. The visualized image of oil flow on a ship model is analyzed. The fractal variable is obtained by the maxima analysis and the good results on the exprement is obtained by the visualized image analysis.

• Molecules and Cells
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• v.26 no.1
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• pp.34-40
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• 2008

To express an increased level of recombinant Mefp1 (marine mussel adhesive protein) in soluble form, we constructed expression vectors encoding truncated OmpA signal peptide-Mefp1 fusion proteins. OmpA signal peptide (OmpASP) is the 21 residue peptide fragment of the 23 residue OmpA signal sequence cleavable by signal peptidase I. We successfully produced increased levels of soluble recombinant Mefp1 (rMefp1) with various deletions of OmpASP, and found that the increased expression was caused by the increased pI of the N-terminus of the fusion proteins (${\geq}10.55$). All the OmpA signal peptide segments of 3-21 amino acids in length had the same pI value (10.55). Our results suggest that the pI value of the truncated OmpASP ($OmpASP_{tr}$) play an important role in directional signaling for the fusion protein, but we found no evidence for the presence of a secretion enhancer in OmpASP. For practical applications, we increased the expression of soluble rMefp1 with $OmpASP_{tr}$ peptides as directional signals, and obtained rMefp1 with the native amino terminus (nN-rMefp1) using an $OmpASP_{tr}$ Xa leader sequence that contains the recognition site for Xa protease.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.5
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• pp.533-539
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• 2012

The purpose of this study is to estimate a validity of control signal through a design of Exoskeleton Robot Arm's capable of intelligent recognition as a human arm's motion by using realtime processed data of generated EMG signals. By an intelligent algorithm, the EMG output value of human biceps and triceps muscles contraction can be recognized and used for the control over exoskeleton arm corresponding to human's recognition and judgement. The EMG sensing data of muscles contraction and relaxation are used as the input signal from human's body to operate the Exoskeleton Robot Arm thus copying human arm motion. An intelligent control of Exoskeleton Robot Arm is to design the analog control circuit which processes the input data, and then to manufacture an integrated control board. And then abstracted signal is passed by DSP signal processing, Fuzzy logic algorithm is designed for a accurate prediction of weight or load through the intelligent algorithm, and design an Exoskeleton Robot Arm to express a human's intention.