• Title/Summary/Keyword: shot hole

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Antibacterial Activity of Streptomyces sp. J46 against Bacterial Shot Hole Disease Pathogen Xanthomonas arboricola pv. pruni (Streptomyces sp. J46의 세균성구멍병원균 Xanthomonas arboricola pv. pruni에 대한 항균 활성)

  • Lee, Jeong Eun;Lim, Da Jung;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.1
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    • pp.20-32
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    • 2021
  • BACKGROUND: Bacterial shot hole of stone fruits is a seriuos plant disease caused by Xanthomonas arboricola pv. pruni (Xap). Techniques to control the disease are required. In this study, microorganisms with antibacterial activity were isolated to develop as a microbial agent against the bacterial shot hole. METHODS AND RESULTS: An isolate with the strongest activity among the isolates was identified as Streptomyces avidinii based on 16S rRNA gene sequence analysis and designated Streptomyces sp. J46. J46 showed suppression of bacterial leaf spot with a control value of 90% at 10 times-diluted cell free supernatant. To investigate antibacterial metabolites produced by J46, the supernatant of J46 was extracted with organic solvents, and the extracts were subjected to chromatography works. Antibacterial metabolites were not extractable with organic solvents. Both reverse and normal phase techniques were not successful because the metabolites were extremely water soluble. The antibacterial metabolites were not volatiles but protein compounds based on hydrolysis enzyme treatment. CONCLUSION: Our study suggests that Streptomyces sp. J46 may be a potential as an microbial agent against bacterial shot hole. Further study to identify the metabolites is required in more detail.

Protection of Peach Trees from Bacterial Shot Hole with Bordeaux Mixture Spray during the Postharvest Season (복숭아 수확후 보르도액 살포에 의한 세균성구멍병 방제효과)

  • 김산영;권태영;김임수;최성용;최충돈;엄재열
    • Research in Plant Disease
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    • v.7 no.1
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    • pp.37-41
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    • 2001
  • This experiment was carried out to investigate the prevention of bacterial shot hole by Bordeaux mixture when it was sprayed on peach trees after harvest. bordeaux mixture was sprayed on \`Mibaeko\` peach trees 1 to 3 times after mid September, and the occurrence of bacterial shot hole was examined in the next year. Bacterial shot hole in leaves appeared from mid May and thereafter increased gradually. The more times was sprayed Bordeaux mixture, the less peaches were diseased with bacterial shot hole. At the beginning of August, the peach harvest time, the disease incidence of the untreated control plot was 27.4 to 38.1%, while the disease incidence was 9.7 to 31.8% when Bordeaux mixture was sprayed. The control value ranged from 16.5 to 64.6%. Occurrence of the fruit disease was similar to that of the leaf disease. Incidence of the fruit disease in the untreated control was 17.2 to 21.6%, but incase of the chemical treatment, it was 5.0 to 12.2 %, showing 41.9 to 70.9% of the control value. Chemical injury on peach leaves were not found in the 4-12 and 408 types, but occurred in some degrees in the 6-6 type of Bordeaux mixture.

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Fatigue Life Prediction and Strength Evaluation of Shot Peened Parts (쇼트피이닝한 부재의 피로수명 예측 및 피로강도 평가)

  • Kim, Hwan-Du;Lee, Sun-Bok
    • 한국기계연구소 소보
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    • s.15
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    • pp.75-87
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    • 1985
  • A review was performed on fatigue life prediction and strength evaluation of shot peened parts. Fatigue strength of machine parts can be improved by shot peening due to compressive residual stresses on such parts. Compressive residual stress cannot be uniquely define by peening intensity. Several measuring methods of residual stress and the principle of hole drilling method are presented. Exploratory measurement of residual stress was performed on the shot peened SM35C plate with the hole drilling method. Fatigue life and failure location of shot peened parts under bending load can be predicted by a damage parameter which is incorporated with material properties, residual stress, and applied stress conditions. Some method are presented to predict the fatigue strength of shot peened parts at any given life. Shot peening gives its full benefit to the notched machine parts of high strength steels.

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The Best Spray Timing for the Control of the Bacterial Shot Hole with Bordeaux mixture (6-6) after Wintering in the Peach Orchard (복숭아 과원에서 월동 후 세균구멍병 방제를 위한 보르도액 최적 살포시기)

  • Kim, San Yeong;Park, Won Heum;Son, Hee Jung;Lee, Suk Hee;Song, Young Woon;Park, So Deuk
    • The Korean Journal of Pesticide Science
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    • v.19 no.2
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    • pp.106-112
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    • 2015
  • This experiment was carried out to investigate the best spray timing for the control of the bacterial shot hole with Bordeaux mixture (6-6) after wintering in the peach orchard. We sprayed Bordeaux mixture (6-6) on 'Mibaekdo', 'Yumyeong' and 'Cheonhong' of peach verities from late-March to mid-April in 2001, 2002 and 2014, and examined how much bacterial shot hole and chemical injure occurred. The infection rate of bacterial shot hole according to spray time was 10.2 to 11.7% at leaves, and 1.7 to 2.2% at fruits in 2002. And Efficacy of the Bordeaux mixture treatment for control of bacterial shot hole at full blooming time 21.2 to 30.4% at leaves, 1.7 to 4.4% at fruits in 2014. The chemical injure occurred slightly for one week hence. The fruit bearing rate was 20.7 to 29.8% at 'Mibaekdo', and 35.4 to 61.9% at 'Yumyeong' peach trees. When the spray at the time of blooming period by bordeaux mixture was investigated the control effect of bacterial shot hole disease. The infected leaves was better than 21 to 25% twice the quantity of finished products and bordeaux self-preparation about 28 to 30% base quantity of finished products, also the control efficacy was higher in infected fruits trends in bordeaux self-preparation method and twice the quantity of finished products.

Shot Hole of Peach and Japanese Plum Caused by Xanthomonas campestris pv. Pruni and Erwinia nigriflens in Korea (Xanthomonascampestris pv. pruni와 Erwinia nigrifluens에 의한 복숭아 및 자두의 세균성구멍병)

  • 최재을;이은정;박영섭
    • Research in Plant Disease
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    • v.6 no.1
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    • pp.10-14
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    • 2000
  • In 1998, bacterial shot hole of peach (Prunus persica) and Japanese plum(Prunus salicina) was found in Naju and Milyang. Five isolates of bacteria isolates from the diseased leaves and fruits of peach and Japanese plum were classified into genus Erwinia and Xanthomonas on diagnostic characteristics. Of five isolates, two were identified as X. campestris pv. pruni, three as E. nigrifluens. E.nigrifluens is the first description of bacteria which causes the disease on peach and Japanese plum in Korea. the symptoms caused by E. nigrifluens were hardly distinguished from those caused by X. campestris pv. pruni. In addition, it was observed that two pathogenic bacteria were isolated from most of naturally infected plants at the same time. from the reason mentioned above, we proposed to use a single common name \"bacterial shot hole of peach and Japanese plum\" for the both bacterial diseases, hereafter.

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A Study on Performance Improvement of Electrical Discharge Machining for Producing Micro-holes Using a Shot Blasting Surface Treatment (쇼트 블라스팅 표면처리를 통한 미세홀 방전가공 성능향상에 관한 연구)

  • Jang, H.S.;Kim, H.S.;Shin, K.H.
    • Transactions of Materials Processing
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    • v.21 no.5
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    • pp.312-318
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    • 2012
  • With an increasing trend toward miniaturization, electrical discharge machining(EDM) has been receiving a lot of attention as a suitable production technology for micro-parts, since it enables the machining of hard conductive materials with a high degree of repeatability and without alteration to the material. When a micro-hole is fabricated by EDM, however, the diameter of the inlet hole is larger than that of the outlet region due to the additional discharge effect caused by the eroded particles. In this paper, a shot blasting surface treatment, in which an abrasive material is accelerated through a pressurized nozzle and directed at the surface of a part, is suggested as an effective method to reduce the tapered shape of EDM micro-hole. In addition, the influence of process parameters such as spark-on time and electrode diameter on the machining performance was investigated. It is shown quantitatively that the difference in diameter between the inlet and outlet holes decreases with the shot blasting treatment and with decreasing spark-on time.

Incidence and Ecology of Major diseases on Peach in Gyeongbuk Province (경북지역 복숭아의 주요 병해 발생 및 생태)

  • 박소득;권태영;임양숙;정기채;박선도;최부술
    • Korean Journal Plant Pathology
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    • v.11 no.3
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    • pp.224-229
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    • 1995
  • Occurrence and incidences of major diseases of peach (Prunus persicae pv. vulgaris), leaf curl caused by Taphrina deformans, bacterial shot hole caused by Xanthomonas campestris pv. pruni, brown rot caused by Monilinia fructicola, and anthracnose caused by Glomerella cingulata in peach orchards in Cheongdo and Kyungsan areas of Gyeongbuk province, Korea, were investigated for four years from 1990 to 1993. In leaf curl and bacterial shot hole which mainly occurred on leaves, frist disease occurrences were dated from late April to early May. The maximum leaf curl incidence was dated in mid May, while dates of the maximum bacterial shot hole incidence varied from mid May to mid August depending on the years surveyed. In brown rot and anthracnose on fruit, the first disease occurrence dates ranged from early June to early August; however, the maximum disease incidences for both were invariably dated in late August. The disease incidences on the dates of the maximum incidences differed year by year, and the averages for the 4 years were 13.2%, 10.5%, 10.9% and 3.8% for leaf curl, bacterial shot hole, brown rot and anthracnose, respectively. Especially in the leaf curl disease, the first disease occurrence dates and the maximum disease incidences matched with the amounts of precipitation of rain up to April, suggesting that the disease occurrence may be related to the precipitation during the early season. The occurrence of leaf curl was somewhat higher in cultivar“Baekmi”than other cultivars. All of the major disease occurred more in hilly orchards than in plain ones.

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Slip Behavior of Friction Type High-Tension Bolted Joints with Oversize Hole (과대공을 갖는 고장력 볼트 마찰이음부의 미끄러짐 거동)

  • Cho, Sun Kyu
    • Journal of Korean Society of Steel Construction
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    • v.9 no.3 s.32
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    • pp.301-307
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    • 1997
  • In field fabrication of steel members, the oversize hole is frequently required due to reaming and mismatching. But, there are no provisions and investigations about oversize hole in the Korean specifications. So, in this study, the tension test of friction type high-tention bolted joints is performed with parameters of bolt hole size, surface treatment and tightening force, and investigate the effect of slip behavior with those parameters. From the results, the enough tightening force is needed to obtain some degree slip load in shot blast treatment case, although tightening force is reduced somewhat, it is no problem to guarantee slip load in zincrich primer case. The slip behavior of joints with oversize hole(26mm) is similar to the slip behavior of joints with hole of nominal size.

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