• Korean Journal of Plant Resources
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• v.8 no.2
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• pp.153-157
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• 1995

Embryogenic callus induces from shoot tip and leaf segment of Zanthoxylum piperitum for producing somatic embryogenesis and plant regeneration were cultured in vitro on Murashige and Tucker's(MT) medium treated with casein hydrolysate $NH_4NO_3$, $KNO_3$ and plant growth regulator. The most effective somatic embryogensis was observed in the medium added by two fold $NH_4NO_3$(3300mg/l)+2. 4-D 0.1mg/l and $KNO_3$(3800mg/l)+2.4-D 0.1mg/l. Also, MT medium supplemented with casein hydrolysate 700mg/l added by 2, 4-D 0.1mg/l were effective in obtainingn somatic embryos from embryogenic callus The effect ofm MT medium supplemented with casein hydrolysate without 2, 4-D was lower than that with (3300mg/l) 2, 4-D for the formation of somatic embryos.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.67-74
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• 2005

To overcome various disadvantages of conventional cul-ture vessels for micropropagation, a novel disposable vessel, the 'Vitron', made of a multi-layered $OTP^{(R)}$ film and supported by a polypropylene frame, was developed. The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability. Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on $3\%$ sugar-containing agar on MS medium and placed at 3000 ppm $CO_2$-enrichment at a low photosynthetic photon flux density (PPFD) ($45{\mu}mol\;m^{-2}\;s^{-1}$). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro and ex vitro plantlets were significantly enhanced in the Vitron compared to those cultured in a polycarbonate vessel. Explants that were cultured on the same MS medium under low PPFD at various $CO_2$ concentrations were also cultured at 3000 ppm $CO_2$- enrichment at various PPFD: 30, 45, 60, 75 and $90{\mu}mol\;m^{-2}\;s^{-1}$. The best in vitro and ex vitro growth obtained for 3000 ppm $CO_2$-enrichment at $75{\mu}mol\;m^{-2}\;s^{-1}$ PPFD. The novel Vitron vessel, when placed under the two conditions, may replace conventional culture vessels for the successful micropropagation of sweetpotato.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.1-6
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• 1999

In order to obtain plantlet derived by anthers, the anthers of Lilium asiatic hybrid 'Gran Paradiso' were cultured on Murashige and Skoog's medium supplemented with various combinations of auxin and cytokinin. The most suitable pollen stage of anther culture for the callus induction was 3 days before anthesis at the early to late binucleate stage. Organogenic calli were induced on MS medium supplemented with 5.0 mg/L 2,4-D alone and the combination of 1.0 mg/L 2,4-D and 1.0 mg/L kinetin, however, the combination of NAA and BA was more effective than that of 2,4-D and kinetin on plant regeneration through organogenesis. Shoots were formed from the induced callus on the medium with 0.5 mg/L NAA and 1.0 mg/L BA after 180 days of culture. Multiple shoots with 3-4 leaves, roots, and bulblets were formed on the medium with the combination of 2.0 mg/L NAA and 2.0 mg/L BA after 250 days of culture. The chromosome from root tip of the regenerated plantlet showed the diploid (2n=2x=24). Diploid plants were transferred to the pots and all plants were flowered in two years.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.10a
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• pp.114-115
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• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.10b
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• pp.16-17
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• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.81-85
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• 2001

The experiment was conducted to know the effect of plant growth regulators on axillary bud elongation from in vitro stem cutting and the possibility of virus-free stock production. Axillary buds were well elongated in 3/4 strength MS medium supplemented with 0.1 or 0.5 mg/1 BA and 0.05 mg/1 NAA. Transferred plantlets could be established well in vermiculite and peat moss mixture (3:1, v/v) compare to other mixtures. In virus indexing, all the varieties of mother plants were infected by GLRV Ⅲ. Infected percentages of the three varieties were ranged from 30% to 75%. But negative response was revealed against the other species of virus, GLRV Ⅰ, GFLV and ArMV. Plantlet of 'Schuyler' and 'Muscat of Alexandria', which were cultured in vitro, showed positive response against GLRV Ⅲ and infected percentage of the former was 37.5% but the latter, 12.5%. On the other hand, that of 'Campbell Early' negativiely responded against all the species of virus indexed.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.501-504
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• 2001

The effect of soil EC on tissue morphology of leaf and shoot tip in cucumber (Cucumis sativus L. cv. Euinchim-baekdadagi) seedlings was investigated. Number of trichomes on leaf upper epidermis increased with the increase in soil EC from 1.0 to $3.0dS{\cdot}m^{-1}$, but the shape and number of stomata on lower epidermis remained unchanged. Epidermal cells of cucumbers grown in EC $1.5dS{\cdot}m^{-1}$ soil was occupied mostly by large vacuole whereas those grown in EC $3.0dS{\cdot}m^{-1}$ soil were filled with a nucleus, mitochondria, chloroplast and other micro-organelles. Sponge parenchima cells were also larger and contained fewer chloroplasts at EC $1.5dS{\cdot}m^{-1}$ than those grown at EC $3.0dS{\cdot}m^{-1}$. Leaf thickness decreased at high EC and the color of epidermal cells became significantly darker on the photograph of optical microscope. Normal tissue differentiation was greatly suppressed in plants grown in soils with $3.5dS{\cdot}m^{-1}$ or higher EC.

• Journal of Bio-Environment Control
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• v.16 no.2
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• pp.115-120
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• 2007

An experiment was conducted to measure rooting and growth of cuttings of rose as affected by cutting length, number of leaflets and cutting position. Test plants were two cultivars of Rosa hybrida 'Red Sandra'(standard) and 'Little Marble'(miniature). Cutting length was 4, 7 or 10cm for 'Red Sandra' and 3, 5 or 7 cm for 'Little Marble'. The number of leaflets left on the five-leaflet leaf was 0, 2, 4 or 5. The cutting position on the stem was between $1{\sim}2,\;3{\sim}4,\;5{\sim}6\;or\;7{\sim}8$ nodes from the shoot tip, which have five leaflet leaves. The most efficient cutting length was 7cm in both cultivars. Treatment with all leaflets left and cutting position at $1{\sim}2$ nodes resulted in good rooting ratio for both cultivars.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.43-49
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• 2014

This study was designed to determine the effect of red LEDs during healing and acclimatization process on the survival rate and quality of grafted tomato seedlings. Red LEDs and no light (darkness) were used for treating three rootstock cultivars, which are 'B-Blocking', 'Kanbarune', 'High-power' in healing room. Results showed that survival rates of grafted seedlings in red LEDs were higher than those in no light treatment. Significant variation on survival rates of rootstock cultivars was observed in no light treatment but there was not significant variation in red LEDs treatment. Light treatment also reduced the percentage of infected plants, except for the 'Kanbarune' cultivar. Seedling quality in red LEDs was better than that in no light treatment by improving growth parameters such as plant height, leaf length, leaf width, leaf area, fresh and dry weight of shoot and root. Light treatments and rootstock cultivars did not affect number of leaves, leaf chlorophyll value and T/R ratio of seedlings, but seedlings in red LEDs were significantly more compact than those in no light treatment. Moreover, the root morphology of seedlings such as total root surface area, total root length, and number of toot tips in red LEDs was also greater than that in no light treatment.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.371-377
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• 2012

This study was conducted to investigate the physiological and structural damages to cadmium (Cd) in Acorus calamus var. angustatus as a native aquatic species in Korea. In addition to the physiological responses such as plant growth, photosynthesis, and root activity, the structural damages in leaf and root tissues were observed through light and scanning electronic microscopy. The five-leaf plants were treated with different Cd concentrations 0, 10, 25, and 50 ${\mu}M$ for 15 days. The plant damages to Cd were significant at 10-25 ${\mu}M$ Cd physiologically and at 25-50 ${\mu}M$ Cd structurally. The physiological damages in the shoot part (photosynthesis) started at 10 ${\mu}M$ Cd whereas those in root part (root activity) were serious above 25 ${\mu}M$ Cd. On the other hand, the structural damages began at 25 ${\mu}M$ Cd in the leaf and root tissues similarly, but the plant tissue destruction was more serious in the roots than in leaves. Based on the plant physiological and structural damages, 10 ${\mu}M$ was assumed to be the limited concentration for sustainable growth and landscaping ability in Acorus calamus var. angustatus to Cd.