• Mycobiology
• /
• v.43 no.3
• /
• pp.311-318
• /
• 2015

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ${\beta}$-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.4
• /
• pp.565-574
• /
• 2011

The development of satisfactory alternatives for supplying the nutrients needed by crops could decrease the problems associated with conventional NPK chemical fertilizers. In this study, the effects of bacterial and fungal effective microorganisms (EM) on the growth of Chinese cabbage (Brassica rapa) were evaluated. This investigation was carried out parrallel with conventional NPK chemical fertilizer and a commercial sold microbial fertilizer to compare between each of their effect. Sterile water and molasses were served as controls. Azotobacter chroococcum effect also was studied either alone or in combination with the effective microorganisms on the growth parameters. In contrast to the bacterial EM, the fungal EM alone without A. chroococcum had a more stimulating effect than fungal EM combined with A. chroococcum. Results showed that seedling inoculation significantly enhanced B. rapa growth. Shoot dry and fresh weight, and leaf length and width significantly were increased by both bacterial and fungal inoculation. The results indicated that the NPK chemical fertilizer deteriorates the microflora inhabiting the soil, while the effective microorganisms either fungal or bacterial ones increased the microbial density significantly. This study implies that both of fungal and bacterial EM are effective for the improvement of the Chinese cabbage growth and enhance the microorganisms in soil. The results showed antagonism occurred between A. chroococcum and each of Penicillium sp and Trichoderma sp in both agar and plant assays. The data were statistically analyzed by ANOVA and Dunnett test.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.4
• /
• pp.215-219
• /
• 2001

This study was conducted to investigate the effect of culture media and environment on PLB proliferation by using PLBs produced from leaf segments excised from shoot of Phalanopsis flower stalk. The fresh weight of PLBs propagated was higher in MS medium than in NDM (New Dogashima medium) or VW, but the condition of PLB was better in NDM medium. Natural additives of Coconut water, potato and apple were absolutely required for the PLB propagation. PLB propagation was better in solid medium than in liquid medium including cotton as support. Optimal sucrose concentration for proliferation was 10 g/L. PLB proliferation was very effective condition 14.3 $\mu$mol.s$^{-1}$ m$^{-2}$ in PPFD and $25^{\circ}C$.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.21 no.2
• /
• pp.77-86
• /
• 2019

To determine none top soil covering in rice seedling nursery method for the sparse machine transplanting, four different sowing methods were tested. Shoot and root length, fresh weight, leaf number and color using leaf color chart(LCC) and SPAD were collected as the data comparison of methods. The seedling height showed the highest growth according to the conventional (230g seed rate of pre-emerged seeds and top-soil covering) > high sowing density 1 (290g seed rate of pre-emerged seeds and top-soil covering) ≥ high sowing density 2(290g seed rate of pre-emerged seeds and none top-soil covering) > high sowing density 3(290g seed rate of iron-coated seeds and none top-soil covering). There was any statistical difference between groups in root length, leaf number, LCC, and SPAD values. Thus, a high sowing density of 290g for rice nursery seedling box was recommended to the sparse machine transplanting in rice cultivation with the none top-soil covering method, enabling convenient handling in transportation and machine transplanting work.

• Journal of Bio-Environment Control
• /
• v.11 no.2
• /
• pp.74-80
• /
• 2002

The effect of the foliar application of amino acid mixture on the growth of melon (Cucumis melo L.) seedlings was investigated. The amino acid treatments were initiated at the first (Ll) or second (L2) fully expanded leaf stage. The concentrations of amino acid mixture used were 0,10, 20, and 30 mg . L$^{-1}$ . At Ll stage, the fresh and dry weights of shoot were high in the amino acid treatments. Plant height was the highest in 30 mg . L$^{-1}$ at the third sampling of Ll. At L2 stage, shoot fresh weight was the greatest when the concentration of amino acid mixture was 30 mg.L$^{-1}$ at the third sampling. Plant height was the highest in 30 mg L$^{-1}$ at the second and third samplings. At the third sampling of Ll stage the amino acid mixture affected leaf length and leaf width of the first true leaf. At the third sampling of L2 stage leaf length was not significantly dirtferent between treatments, while leaf width was greater in amino acid treatments. At the second and third samplings of Ll stage the amino acid mixture had effect on leaf length and leaf width of the second true leafs which were not significantly different between treatments at L2 stage. Leaf length and leaf width of the third true leaf were affected by amino acid treatments. In conclusion, when the first true leaf expanded\ulcorner three foliar applications of 20-30 mg . L$^{-1}$ amino acid mixture can stimulate the growth of melon seedlings. If the amino acid mixture is sprayed at the second true leaf stage, two foliar applications of 30 mg . L$^{-1}$ amino acid mixture can improve the growth of melon seedlings.

• Horticultural Science & Technology
• /
• v.18 no.4
• /
• pp.523-528
• /
• 2000

Plug seedlings of Petunia hybrida 'Madness Rose' and Viola tricolor 'Magestic GT' were cultured in media containing various volume ratios of recycled plug medium, recycled coir, perlite, granular rockwool, and vermiculite for 36 and 43 days after sowing, respectively. Recycled plug medium and recycled coir were steam pasteurized for 30 minutes at $120^{\circ}C$ and 1.5 atmosphere. An unused commercial plug medium (Tosilee, pH 5.10, EC $0.12mS{\cdot}cm^{-1}$ at 1:5 dilution, v/v, Shinan Grow Co.) was used as the control. The pH of different media before and after growing seedlings was similar. Medium EC was high when recycled plug medium was included. Recycled coir (75%)＋vermiculite (25%) mixture also had high medium EC. However, medium EC was low when granular rockwool or perlite was included. Height, root formation, shoot dry weight and leaf count (ea) of petunia, and height, total fresh and dry weights, and shoot fresh and root dry weights of pansy were the highest in recycled coir (75%)＋perlite (25%) mixture. Recycled coir was better than recycled plug medium in physicochemical properties, and also in resultant plant growth. It is recommended to include perlite or granular rockwool when plug media including recycled horticultural media are prepared.

• Korean Journal of Weed Science
• /
• v.8 no.1
• /
• pp.23-27
• /
• 1988

This experiment was conducted to determine germination ability, life cycle, regrowth ability of Portulaca oleracea L. The newly harvested seeds from P. oleracea flowered before July 15 were able to germinate, but percent germination decreased markedly as the flowering time was later than July 15, showing no germination of seed collected at Sept. 12 which were flowered at August 30, 76.5% of seeds can be germinated in the light as seed stored in room condition for 90 days, but in dark condition, it needed 2 years of seed storage for germination. Low temperature treatment at $2^{\circ}C$ for 5 days enhanced seed germination of P. oleracea in both light and dark conditions. The maximum vagetative growth was observed at 30 to 75 days after seeding. The late seeding time such as July 1 shortened the period of vegetative growth. However, regardless of the seeding times, the first flowering was observed at about 40 days after seeding. Leaf numbers, shoot lengths, fresh and dry weights were greatly affected by the seeding dates, showing that the earlier seedings produced significantly higher shoot length, fresh and dry weight, leaf numbers and branch numbers than those of the late seedings. When all branches were removed on 68 days after seeding, their regrowth ability was 50.3% and cuttaged branches showed 78.1% regrowth as compared to intact plant.

• Journal of Bio-Environment Control
• /
• v.5 no.2
• /
• pp.236-247
• /
• 1996

The main objective of this study was to evaluate the effectiveness of nutrient solutions, substrates, and nutrient solution concentrations in substrate culture of radish(Raphanus sativus L. var. sativus). Cooper's, Hoagland ＆ Arnon' 5, and Yamazaki's solution were used to determine the most suitable nutrient solution in deep flow culture(DFC). In result, Yamazaki's solution treatment showed better results than Hoagland's and Cooper's solution treatments in leaf length, leaf number, shoot and root fresh weights. Cooper's solution was much worse than others. Root shape index were low as 0.6 in all treatments. The selection of suitable was conducted among 14 kinds of substrates which were used commercially, such as sand, perlite and peatmoss, in substrates culture. Sand was the most proper in radish growth and shortened the growth periods. Sand also showed better results then others in leaf length, leaf number, shoot and root fresh weight. On the contrary, radish growth in peatmoss was the worst. Generally, root shape index was higher in substrate than in DFC. In order to investigate the suitable ionic strength in radish, Yamazaki's solution was treated with EC of 0.5, 1.0, 1.5, and 2.0 mS/cm. Generally radish growth above 1.0 mS/cm concentration was good, and the best result was shown in 1.5 mS/cm. Vitamin C contents were not significantly different in the roots of radish grown under 1.0 mS/cm or more. The highest vitamin C content was shown in 0.5 mS/cm, and so was thiocyanate content. Anthocyanin contents increased with the increase of the ionic strength in nutrient solution. Mineral nutrient contents had no significant statistical differences between the treatments, but potassium content was remarkably high in 1.5 mS/cm.

• Korean Journal of Weed Science
• /
• v.14 no.1
• /
• pp.62-70
• /
• 1994

This study was conducted to find out if there are some differences in germination, growth and morphological characteristics between rice(Oryza saliva) and barnyardgrass(Echinochloa crus-galli) under various cropping patterns and to abtain the fundamental information on weed control method in direct seeded rice. Rice was broadcast on soil-surface(Broadcast rice), drill seeded in soil(Drilled rice) and barnyardgrass was drill seeded in soil(Barnyardgrass) under dry(Dry condition) and water direct seeded condition(Water condition). Also rice was transplanted with 8-day seedlings(8 -day-old seedling) and 25-day seedlings(25-*day-old seedling) under transplanting condition(Transplanting condition) At 1, 2, 3, 5, 7, 10, 15 and 20 days after seeding or transplanting(DAS/T), plants were harvested to examine their germination, growth and morphology. The major results were as follows ; Until 5 DAS/T growth of rice and barnyardgrass were well established under dry condition but under water condition growth of shoots was mainly elongated. At 20 DAS/T barnyardgrass had greater plant height and shoot fresh weight than rice under direct seeded condition, while plant height and shoot fresh weight of rice was greater than those of barnyardgrass under transplanting condition. Root length of barnyardgrass was greater under the dry, drilled, direct seeded conditions than that of rice under the water, broadcast, transplanting condition, respectively. And root fresh weight of rice under direct seeded condition was similar to that of barnyardgrass but that of rice under transplanting condition was significantly greater than that of barnyardgrass. Barnyardgrass only formed mesocotyls and its length increased with increased depth of seeding. Leaf stage and leaf area of barnyardgrass was greater under the dry, drilled than those of rice under water, broadcast conditions, respectively, while those of rice was greater than those of barnyardgrass under transplanting condition. Chlorophyll contents were higher in barnyardgrass, dry direct seeded rice, transplanted rice, water direct seeded rice in descending order.

• Korean Journal of Medicinal Crop Science
• /
• v.10 no.5
• /
• pp.344-352
• /
• 2002

This study was conducted to determine the optimum planting dates and density of one year old Bupleurum falcatum L. to improve its quality. Furthermore, the effect of cultural methods and plant growth regulators$(GA_3,\;IAA,\;Kinetin)$ on the quality of B. falcatum were also investigated. In this study, Jeongseon cultivar collected in Korea and Mishima cultivar introduced from Japan were used. Some of the results obtained are as follows. Jeongseon cultivar showed less stem branches and shoot weight compared to Mishima. However, Jeongseon cultivar showed tall plant height, high root fresh and dry weight, and high levels of SSa and TSS, but low SSc content than that of Mishima. Although fresh and dried root weight of both cultivars were not affected by plant growth regulators treatment. compared with the untreated one, increasement of TSS content were made by $GA_3$ 100 ppm, IAA 50 ppm or 10 ppm and kinetin 10 ppm treatment in Jeongseon cultivar, and by $GA_3$ 10 ppm and IAA 100 ppm or 10ppm treatment in Mishima cultivar applied on June 1.