• Journal of Plant Biotechnology
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• 제29권1호
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• pp.51-57
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• 2002

대두의 재분화 체계를 확립하고자 품종별 shoot 형성률, 적정배지 및 적정조직을 구명하고자 하였다. 유묘의 조직부위별 비교에서는 1개의 자엽을 포함한 절에서 multiple shoot 형성률이 높았다. 기본배지에서는 1/2 B5배지가 효율적이었으며, TDZ 처리시에 multiple shoot 형성률이 높았으며, 처리농도는 2mg/L가 효율적이었다. Shoot 형성시 자엽 네 전분은 배양 1주까지 증가 후 감소하였으며, 수용성 당은 배양 2주까지 증가 후 shoot가 형성되는 배양 4주에는 관찰되지 않았다. 품종 간 비교에서는 품종에 따라 shoot 형성 양상이 구분되어, '흑청콩' 등 11품종에서는 multiple shoot 형성률이 높았고, '만리콩' 등 16품종에서는 pair shoot 형성률이 높았다. 자엽절에서 shoot가 형성되는 양상에 따른 조직화학적 관찰 결과 pair shoot의 경우 내생적으로 잠재한 액아에 의한 것이며, multiple shoot의 경우는 새로이 형성된 shoot로 확인되었다.

• 한국연초학회지
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• 제13권1호
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• pp.36-42
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• 1991

The present study was conducted to obtain some basic information on the shoot formation from crown gall callus and the characteristics of teratoma shoot derived from crown gall induced by inoculation of Agrobacterium tumefaciens C58. Crown gall callus could be continuously cultured on the phyohormone free basic medium. The growth of crown gall callus was inhibited when BA were added to the cultural media. Shoot formation from crown gall callus fail to be initiated except teratoma shoot which induced on the phytohormone free medium after several subculture on rare occasions. Teratoma shoot could not form root and grow as normal shoot. Addition of BA to cultural media was not effective for shoot elongation, reduction in multiple shoot formation, but IBA was somewhat effective for shoot elongation of teratoma shoot, never for root formation.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.95-100
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• 2001

To select the section with shoot formation ability, the calli and shoot formation from three sections (first leaf including cotyledonary node, hypocotyl and cotyledon explants) of 5-days-seedlings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section (10%) and the cotyledon section (0%). The shoot formation rates and shoot number of the four excised sections (whole first leaf, a half of the first leaf, a third of the first leaf and only node) of the first leaf were also investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and numbers (3-4 / explants) than those in other two explants. Effects of six cytokinins (kinetin, zeatin, BAP, 2iP, PBA, and TDZ) on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0 mg/L) exhibited the highest in shoot formation rate (94%) and numbers (8 / explant). In addition, the combined effects of three cytokinins (zeatin, BAP, and TDZ; 0.5, 1.0, 2.0 mg/L, respectively) and an auxin (IAA; 0.0, 0.5, 1.0, 2.0 mg/L) were determined. The combination (1:1, v/v) of zeatin (1.0 mg/L) and IAA (1.0 mg/L) exhibited the highest in shoot formation rate (96%) and numbers (16 / explant), twice more than zeatin (1.0 mg/L) alone. The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at various concentrations. The highest root formation (8 / shoot) was achieved on the medium supplemented with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.161-161
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• 2022

This study aimed to investigate the effect of growth regulators on the formation of the organ in the in vitro propagation of diploid and tetraploid Codonopsis lanceolata, and gain the basic data for in vitro propagation of superior C. lanceolata. In the case of diploid C. lanceolata, the highestshoot formation (3.0) was observed at 0.5 mg·L^-1 addition medium with low IBA concentration. The shoot formation of tetraploid C. lanceolata was suppressed by addition of IBA. In the addition of lAA, the shoot formation of diploid C. lanceolata was slightly higher at 1.0 mg·-L^-1 addition medium than that of control group, whereas tetraploid C. lanceolata showed the highest number (5.4) from control group. In the case of NAA, the shoot formation of diploid and tetra C. lanceolata tended to decrease at higher concentration. In terms of BA addition, the shoot formation of diploid C. lanceolata was increased by the addition of BA, whereaswhile the growth of shoot was decreased by the addition of BA. In the case of tetraploid C. lanceolata, shoot was found to be formed by the addition of low concentration of BA, and the growth of shoot was inhibited with the higher addition concentration of BA. With the addition of kinetin, the shoot formation of diploid C. lanceolata was slightly higher than that of control group, and the formation of adventitious root was highest (5.3) in the control group. In the case of tetraploid C. lanceolata, the shoot formation was similar in all treatment groups, but the formation and growth of adventitious root were significantly lower than that of diploid C. lanceolata. In the case of TDZ addition, the shoot formation of diploid C. lanceolata showed the pronounced results at 5.0 mg·L^-1 addition medium, and the growth of shoot was inhibited by the addition of TDZ. The formation of adventitious root was 5.3 and 4.9 in the control group and 0.1 mg·L^-1 addition medium respectively. The formation of the shoot of tetraploid C. lanceolata showed better results with the higher concentration of TDZ, and the growth was better with the lower concentration of TDZ. The formation and growth of adventitious root were significantly slower than that of diploid C. lanceolata.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.169-169
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• 2017

Medium composition plays a key role on influencing organogenesis in plant tissue culture. This study was carried out to examine the effects of medium composition on organogenesis in diploid and tetraploid Codonopsis lanceolata and obtain in-vitro mass propagation of superior species of C. lanceolata. Diploid C. lanceolata was found to be declined regarding MS medium composition for each concentration. However, shoot and adventitious root formation were suppressed with higher mineral salt concentration, and active growth of shoot and adventitious root was exhibited as 4.9 cm and 3.2 cm respectively in 1/2 MS medium. While in tetraploid C. lanceolata, it showed 2.9 cm and 3.2 cm respectively in 1/4 MS medium. In the case of sucrose concentration, no consistent decrease was observed for growth of shoot and the adventitious root of diploid both at high and low concentration. The growth of shoot (at 3% concentration) and adventitious root (at 7% concentration) was 2.3 cm and 2.0 cm respectively. Although there was no difference in shoot formation of tetraploid C. lanceolata in all concentrations with the range of 1.7~1.8, there was a slight decrease in shoot growth at high concentration. Results revealed that the adventitious root formation was suppressed at high concentration. The concentration of agar exhibited no significant difference in shoot formation of diploid C. lanceolata at all concentrations. The maximum result of adventitious growth (4.1 cm) was observed at 0.8% concentration. Slight inhibition of shoot formation and root formation of tetraploid C. lanceolata was observed at higher concentration. Shoot formation of diploid C. lanceolata also exhibited inhibition at higher concentration. Shoot formation of diploid C. lanceolata was increased at lower pH and shoot growth was the highest (2.3 cm) at pH 3.8. Adventitious root formation was higher at lower pH. However, both the adventitious root formation and growth exhibited comparatively higher result at pH 5.8. Taken together, the levels of pH had an effect on shoot and root formation in diploid and tetraploid of C. lanceolata

• 식물조직배양학회지
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• 제25권1호
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• pp.63-68
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• 1998

정단 및 마디조직을 이용한 지황의 기내증식방안을 마련하기 위하여 일련의 시험을 실시하였다. 재생된 신초의 정단을 재배양하였을 경우 BA 5.0 mg/L에서 7.8개의 신초가 형성되었으며 투명화된 묘의 발생은 Gelrite 보다는 Bacto-agar 첨가구에서 감소하였다. 광도와 한천의 농도가 증가할수록 신초형성수 및 생장은 억제되었고 생체중 : 건물중의 비율도 감소하였다. 광도가 2000 lx일 때는 Bacto-agar 0.6-1.2%, 3,000 lx에서는 한천 0.4-0.6%에서 건전한 신초를 10개 이상 생산할 수 있었다. 활성탄소 0.1-0.3%첨가는 신초의 생장촉진과 뿌리형성에 촉진적으로 작용하였으며 투명화 발생률도 감소시켰으나 신초형성수는 현저히 감소시켰다. MS배지내 첨가되는 다량원소의 농도를 0.8-1.0배 하였을때 신초형성수 및 생장이 촉진되었다. 기관형성에 미치는 오옥신의 종류 및 농도별 효과를 조사한 결과 NAA나 IBA 보다는 IAA 0.3mg/L 와 BA 5.0mg/L를 혼합처리 하였을 때 증식률이 16배에 달하였다.

• 한국자원식물학회지
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• 제19권2호
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• pp.308-314
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• 2006

구기자나무로부터 캘러스의 형성과 shoot의 형성에 미치는 생장조절제의 영향을 연구한 결과 캘러스의 형성은 2,4-D를 포함하는 배지에서 효과적이었으나 shoot의 형성에는 억제효과를 보였다. Shoot의 형성은 0.1mg/L IAA + 0.1mg/L BA의 배지에서 형성률과 절편당 형성 개체수가 가장 높은 분화율을 보였다. 절편체의 부위에 극성에 다른 캘러스의 형성과 shoot의 재분화에 있어서는 자엽의 기부와 말단부를 20% 절제한 절편의 기부의 절단면에서는 shoot의 형성이, 하부의 절단면에서는 캘러스의 형성이 높았으며 시간이 경과함에 따라 하부에서도 shoot의 형성이 보였다. 재분화식물은 1/3 MS 배지에서 뿌리가 양호하게 발달하였으며 토양 순화과정에서도 90% 이상의 생존율을 보여 개화가 이루어졌다.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.103-106
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• 2004

Plantlet regeneration from the shoot apex was studied in three different genotypes of the chinese yam (Dioscorea opposita Thunb) cv. Jnagma and Danma, Dunggunma. The effects of plant growth regulators and inorganic salts concentration of the culture medium on bud induction and shoot growth were examined. The combinations of 0.2 mg/L BAP + 0.2mg/L kinetin, 0.01mg/L NAA + 0.2 mg/L kinetin and a single treatment of 0.2mg/L BAP were equally effective for bud and shoot formation from the shoot apices in the three cultivars. Auxin (2,4-D, NAA) treatment enhanced calli formation from the cultured apices. Also, the shoot apices of the cv. Dunggunma produced more callus and buds on the culture medium (MS) containing 0.05mg/L NAA and 0.5-1.0mg/L SAP. Lower salt strength of medium inhibited shoot elongation but did not have much effect on the shoot and bud induction from the shoot apices. These results will be useful to obtain disease-free plants of the Chinese yam.

• Journal of Plant Biotechnology
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• 제4권1호
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• pp.33-37
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• 2002

Micropropagation and adventitious root production via the culture of Polygonatum odoratum were performed. Stem segments of seedlings of Polygonatum odoratum were the most efficient explants for adventitious shoot formation compared to leaf and root segments. Exogenous cytokinin treatment was required for adventitious shoot formation. Among the cytokinin (BA, Kinetin and Zeatin) tested, BA was most effective for shoot formation from stem segments. Auxin (NAA or IBA) in combination with cytokinin significantly enhanced adventitious shoot formation. Twenty five percent of explants produced adventitious shoots on medium with 2.0 mg/L BAP alone, while 83% of explants produced adventitious shoots on medium with the combination of 2.0 mg/L BAP and 0.1 mg/L IBA. Rooting of adventitious shoots was achieved after transferring to 112 MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L zeatin. When stem segments were cultured on MS medium with various kinds of auxin (IBA, NAA and 2,4-D), adventitious roots were formed from callus. frequency of adventitious root formation was highest in 2,4-D than NAA and IBA. When roots were in clusters together with parental stem segments, growth of roots actively occurred in hormone-free MS liquid medium. The above results represent that possible application for the mass production of roots and plantlets through in vitro culture system of Polygonatum odoratum.

• 생명과학회지
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• 제9권5호
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• pp.564-569
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• 1999

This study was conducted to determine the concentrations of plant growth regulators required for regeneration and the concentrations of antibiotics for the selection of transformed regenerants from lettuce, musk melon and tomato. The optimal concentrations of plant growth regulators for shoot formation were NAA 0.1 mg/$\ell$ +BA 0.1 mg/$\ell$ for lettuce, NAA 0.01 mg/$\ell$ +BA 2.0 mg/$\ell$ for melon and NAA 0.1 mg/$\ell$ +BA 0.5 mg/$\ell$for musk melon. Shoot induction from tomato, lettuce and melon was completely inhibited by 30 mg/$\ell$ or higher concentrations of kanamycin. Shoot formation from mu나 melon was not affected by kanamycin up to 40 mg/$\ell$, but was reduced in the presence of 50 mg/$\ell$ and completely inhibited by 100 mg/$\ell$. Shoot formation of all four crops was completely inhibited by higromycin at 10 mg/$\ell$. Both carbenicillin and cefatoxinme did not show any negative effects on shoot formation.