Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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The Effects of Growth Regulators and Medium Strength on the Shoot and Bud Formation from the Shoot Apex of Chinese Yam (Dioscorea opposita Thunb)

  • Shin, Jong-Hee (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Kang, Dong-Kyoon (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Park, Sang-Zo (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Lee, Bong-Ho (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Sohn, Jae-Keun (Dept. of Agronomy, Division of Bioscience, Kyungpook National University)
  • Published : 2004.06.01
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Abstract

Plantlet regeneration from the shoot apex was studied in three different genotypes of the chinese yam (Dioscorea opposita Thunb) cv. Jnagma and Danma, Dunggunma. The effects of plant growth regulators and inorganic salts concentration of the culture medium on bud induction and shoot growth were examined. The combinations of 0.2 mg/L BAP + 0.2mg/L kinetin, 0.01mg/L NAA + 0.2 mg/L kinetin and a single treatment of 0.2mg/L BAP were equally effective for bud and shoot formation from the shoot apices in the three cultivars. Auxin (2,4-D, NAA) treatment enhanced calli formation from the cultured apices. Also, the shoot apices of the cv. Dunggunma produced more callus and buds on the culture medium (MS) containing 0.05mg/L NAA and 0.5-1.0mg/L SAP. Lower salt strength of medium inhibited shoot elongation but did not have much effect on the shoot and bud induction from the shoot apices. These results will be useful to obtain disease-free plants of the Chinese yam.

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References

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