• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.461-465
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• 1998

The suspension culture of an anchorage-dependent cell line (Bok-l) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth ($6.0\times10^6cells/m\ell$) and viability (>80%) at 10 rpm were higher than that at 0 rpm ($1.9\times10^6cells/m\ell$, 65~80%) and 30 rpm ($1.8\times10^6cells/m\ell$ 40~76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.

• Water Engineering Research
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• v.1 no.1
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• pp.83-92
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• 2000

Jet flow can occur by gate opening at downstream of a hydraulic structure such as weir of drainage gate. If the stream bed is not hard or the bed protection is not sufficient, vortex erosion occurs and a resulting scour hole will be formed due to the high shear stress of the jet flow. Once the scour hole is formed, a vortex occurs in ti and this vortex causes additional erosion. If this erosion continues and reaches to the hydraulic structure, it can undermine the bottom of the hydraulic structure and this will lead to failure of the structure itself. Thus, it is necessary to define the physical features of the vortex structure in the scour hole for the design of the bed protection. This study presents the turbulent vortex structure in the scour hole by the gate opening of the hydraulic structure. Characteristics of vortex motion, circulation, vortex scale and vortex were analyzed through experiments. Experimental results of the vortex velocity were compared with theoretical ones. From these, circulation and vortex scale were obtained with known values of inflow depth, inflow velocity and scale of scour hole

• Journal of computational fluids engineering
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• v.13 no.4
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• pp.107-113
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• 2008

The present study numerically simulates the flow and heat transfer characteristics of rib-induced secondary flow in a square cooling channel with staggered V-shaped ribs, extruded on both walls. The rib pitch-to-height ratio (p/h) varies from 2.8 to 10 with the rib-height-to-hydraulic diameter ration (h/$D_h$)of 0.07 and the Reynolds number of 50,000. Shear stress transport (SST) turbulence model is used as a turbulence model. Computational results show that complex secondary flow patterns are generated in the channel due to the snaking flow in the streamwise direction for all tested cases. In the range of p/h=5 to 10 the staggered V-shaped rib gives about 3 times higher heat transfer augmentation than the reference smooth pipe with high heat transfer on both front side and the area around the leading edge of the ribs, while the former cases give about 18 times higher streamwise pressure drop than the latter ones. However, for the thermal performances, based on the equal pumping power condition, the case of p/h=2.8 gives the best result among three cases, mainly due to relatively low streamwise pressure drop, although it gives relatively low heat transfer augmentation.

• Journal of computational fluids engineering
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• v.13 no.4
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• pp.66-71
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• 2008

This research computes the viscous flow field and aerodynamics around the model of a commercial passenger airplane, Boeing 747-400, which cruises in transonic speed. The configuration was realized through the reverse engineering based on the photo scanning measurement. In results, the pressure coefficients at the several wing section on the wing surface of the airplane was described and discussed to obtain the physical meaning. The lift coefficient increased almost linearly up to $17^{\circ}$. Here the maximum lift occurred at $18^{\circ}$ according to the angle of attack. And the minimum drag is expected at $-2^{\circ}$. The maximum lift coefficient occurred at the Mach number 0.89, and the drag coefficient rapidly increased after the Mach number of 0.92. Also shear-stress transport model predicts slightly lower aerodynamic coefficients than other models and Chen's model shows the highest aerodynamic values. The aerodynamic performance of the airplane elements was presented.

• Journal of the Korea Institute of Military Science and Technology
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• v.11 no.2
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• pp.144-151
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• 2008

An experimental study has been conducted to investigate the effects of circular damage hole on the characteristics of airfoil performance. The damage on a wing created from a hit by anti-air artillery was modeled as a circular hole. Force balance measurements and static pressure measurements on the wing surface were carried out for the cases of having damage holes of 10% chord size at quarter chord and/or half chord positions. All experiments were conducted at Reynolds number of $2.85\times10^5$ based on the chord length. The surface pressure data show big pressure alterations near the circular damage holes. This abnormal surface pressure distribution produces shear stress that could lead to the acceleration of the structural degradation of the wing around the circular damage hole. However, in spite of the existence of circular damage holes, the measured force data indicated the only a slight decrease in lift accompanied by increase in drag compared to the results of undamaged one. The influence of damage hole on the aerodynamic performance was increased as the location of damage moved to the leading edge. The effect on the control force was insignificant when the damaged size was not large.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.10
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• pp.1033-1041
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• 2008

The purpose of this paper is to investigate natural frequencies of tapered thick plate with concentrated masses subjected to in-plane force on pasternak foundation by means of finite element method and providing kinetic design data for mat of building structures. Finite element analysis of rectangular plate is done by using rectangular finite element with 8-nodes. For analysis, plates is supported on pasternak foundation. The Winkler parameter is varied with 10, 102, the shear foundation parameter is 5. The taper ratio is applied as 0.0, 0.25, 0.5 and the ratio of the concentrated mass to plate mass as 0.25, 0.5 respectively. As results, we can see that when stiffener's sizes or foundation parameter are larger, the natural frequency increases, and when the concentrated mass or taper ratio or in-plane stress is larger, the natural frequency decreases.

• Journal of the Korea Institute of Building Construction
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• v.8 no.2
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• pp.113-119
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• 2008

Recently, fiber reinforced polymers(FRP) composite materials are used extensively in the rehabilitation of concrete structural members. A main application is to wrap beams and columns using the continuous fibers sheets to improve their strength and ductility. The corner chamfering affects significantly the performance of the continuous fibers sheets, and could lead to environmental problem with waste and dust. The main purpose of this paper is to verify the effect of corner conditions on the strength of the continuous fiber sheets, and to introduce new attached components which can avoid environmental problem. A total of 15 specimens were tested and carefully checked for three types of continuous fiber sheets(carbon, glass, and aramid) and three types of corner conditions(non-chamfering, chamfering, and device attaching). It is proved that the devices proposed in this research have some capabilities to use for RC member. But additional research will be needed for commercializing.

• Geotechnical Engineering
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• v.10 no.4
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• pp.119-132
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• 1994

Undrained cyclic triaxial tests were performed on silt contained in the sand in order to investigate the effect of silt contents on the liquefaction strength and shear characterist ifs of the sand. As the result of this experiment, the weakest percentage of silt contained in the sand was 30% for all the relative density considered in the test. Also, the same bests were performed to find the effect of cyclic speed applied ranging from 0.1Hz to 5Hz on the liquefaction strength. The more the silt is contained in the sand, the greater the liquefaction strength was affected by cyclic speed, While the silt -containing sand was far less influenced by the cyclic speed than clay containing sand. These results are believed to be caused by the change of pore water pressure of the effective stress path.

• Geotechnical Engineering
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• v.6 no.1
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• pp.43-58
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• 1990

In order to get ultimate pullout resistance of ground anchor, the position of failure surface, normal stress and friction angle on the failure surface should be known. In this study, the position of failure surface is obtained by observing deformation of ground around anchor, and stresses on the anchor surface are analyzed by measuring normal and shear stresses on the anchor surface through model anchor test in plane strain. In addition, the relationship between lateral earth pressure and the position of failure surface is analyzed and the formula for calculating ultimate pullout resistance of anchor is proposed by using non-dimensional coefficient of ultimate pullout resistance.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.1115-1122
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• 2011

Reinforced-Roadbed materials are usually composed of crushed stones. Repeated load application can induce deformation in the reinforced-roadbed layer so that it causes irregularity of track. Thus it is important to develop a prediction model of elastic modulus based on stress-strain relation under repeatitive load in order to investigate behavior of reinforced roadbed. The prediction model of elastic modulus of the material can be obtained from repeated triaxial test. However, a proper size of the sample for the test must be used. In this study, a large repeatitive triaxial test apparatus with the sample size of diameter of 30 cm and height of 60cm was adapted for performing test of the crushed stone reinforced-roadbed considering large particle size to get resilient modulus Mr. The obtained resilient modulus was compared to shear modulus obtained from mid size resonant column test. The sample size effect is somewhat large enough so that it is required to design a scale factor based on similarity law in order to use smaller samples for getting elastic modulus of the crushed stone reinforced-roadbed material. A scale factor could be obtained from this study.