• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.26 no.11
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• pp.831-835
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• 2013

In this study, we fabricated a micro $Pb(Zr,Ti)O_3$ (PZT) film piezoelectric cantilever with a Si proof mass and dual beams through MEMS process. The size of the beam and the integrated Si proof mass were about $4,320{\mu}m{\times}290{\mu}m{\times}12{\mu}m$ and $1,380{\mu}m{\times}880{\mu}m{\times}450{\mu}m$ each. To reduce the air damping and have the larger displacement of dual beams was used for design. After mounting micro PZT film piezoelectric cantilever on shaker, we measured the resonance frequency and a output voltage while making resonant frequency changed. The resonant frequency and the highest average power of the cantilever device were 110.2 Hz and 0.36 ${\mu}W$ each, at 0.8 g acceleration and 23.7 $k{\Omega}$ load resistance, respectively.

• Smart Structures and Systems
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• v.25 no.1
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• pp.97-104
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• 2020

Due to the superior properties of nanoparticles, using them has been increased in concrete production technology. In this study, the effect of zinc oxide (ZnO) nanoparticles on the mechanical and smart properties of concrete was studied. At the first, the ZnO nanoparticles are dispersed in water using shaker, magnetic stirrer and ultrasonic devices. The nanoparticles with 3.5, 0.25, 0.75, and 1.0 volume percent are added to the concrete mixture and replaced by the appropriate amount of cement to compare with the control sample without any additives. In order to study the mechanical and smart properties of the concrete, the cubic samples for determining the compressive strength and cylindrical samples for determining tensile strength with different amounts of ZnO nanoparticles are produced and tested. The most important finding of this paper is about the smartness of the concrete due to the piezoelectric properties of the ZnO nanoparticles. In other words, the concrete in this study can produce the voltage when subjected to mechanical load and vice versa it can induce the mechanical displacement when subjected to external voltage. The experimental results show that the best volume percent for ZnO nanoparticles in 28-day samples is 0.5%. In other words, adding 0.5% ZnO nanoparticles to the concrete instead of cement leads to increases of 18.70% and 3.77% in the compressive and tensile strengths, respectively. In addition, it shows the best direct and reverse piezoelectric properties. It is also worth to mention that adding 3.5% zinc oxide nanoparticles, the setting of cement is stopped in the concrete mixture.

• Smart Structures and Systems
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• v.25 no.1
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• pp.57-64
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• 2020

The nonlinear dynamics of a directly forced clamped-clamped-free-free magneto-rheological elastomer (MRE) sandwich shell has been experimentally investigated. Experiments have been conducted on an aluminium shallow shell (shell A) and an MRE-aluminium sandwich shallow shell with single curvature (shell B). An electrodynamic shaker has been used to directly force shells A and B in the vicinity of their fundamental resonance frequency; a laser displacement sensor has been used to measure the vibration amplitude to construct the frequency-response curves. It was observed that for an aluminium shell (shell A), that at small forcing amplitudes, a weak softening-type nonlinear behaviour was observed, however, at higher forcing amplitudes the nonlinear dynamical behaviour shifted and a strong hardening-type response occurred. For the MRE shell (shell B), the effect of forcing amplitude showed softening at low magnetic fields and hardening for medium magnetic fields; it was also observed the mono-curved MRE sandwich shell changed dynamics to quasiperiodic displacement at some frequencies, from a periodic displacement. The presence of a magnetic field, initial curvature, and forcing amplitude has significant qualitative and quantitative effects on the nonlinear dynamical response of a mono curved MRE sandwich shell.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 1997.11a
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• pp.33-34
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• 1997

장비나 부품이 비행 또는 수송 등 실제상황에서 겪게되는 진동환경에 얼마만큼 견디는지 여부를 확인하고, 예상되는 동적 하중에 의한 성능저하나 오 동작이 일어나지 알는지 확인하기 위해 환경진동시험(environmental vibration test)이 수행된다. 이러한 환경진동시험의 경우에는 대형(대용량)의 진동시험기(shaker 또는 exciter)가 이용되며 진동시험기로부터 에너지를 시험물에 기계적으로 전달시켜줄 수 있는 진동 시험치구(이하, "치구(fixture)")가 필요하게 된다. 따라서 치구의 설계문제가 대두되며, 환경진동시험의 성공여부를 좌우하는데 대단히 중요한 역할을 하게 된다. 치구의 설계시 가장 중요한 점은 시험규격에 정해진 기준스펙트럼(specified reference spectrum)이 치구 위에 설치될 여러 시험물부착점들에 그대로 전달될 수 있는 강체치구(rigid fixture)를 설계하는 것이 가장 이상적이지만 치구의 공진 및 반공진 특성으로 인해 시험물부착점들 마다 스펙트럼이 달라지게 된다. 따라서 시험물은 과대시험(overtest) 또는/그리고 과소시험(undertest)를 겪게된다.최근의 진동시험제어 기법으로서는 다채널시스템을 이용한 여러 시험부착점들에서의 진동레벨의 평균을 제어하는 평균제어기법(average control technique)이 이용되므로서 시험주파수 범위에서 공진진동수(resonance frequency)들은 기준스펙트럼과 동일하게 제어가 가능하나 반공진 진동수(antiresonance frequency)들에서는 반공진 진동수들이 갖는 물리적 특성으로 인해 기준스펙트럼과 동일하게 제어가 이루어지지 않으므로시험규격에 정해진 정확한 진동시험이 수행되지 못하게된다.본 연구에서는 치구의 설계단계에서 치구 위의 여러 시험물 부착점들- 평균제어점(average control points)- 에서의 반공진 진동수들을 고려하여 그 감도를 게산하고, 치구의 구조변경을 수행하여 시험물 부착점들에서의 반공진 진동수를 일치시키므로서 종래의 진동시험제어시 나타나는 반공진진동수에서의 문제점을 제거할 수 있고, 그 결과 시험물 부착점들에서의 스펙트럼이 시험규격에 정해진 스펙트럼대로 진동시험이 수행될 수 있게 하는데 있다.

• Environmental Analysis Health and Toxicology
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• v.26
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• pp.12.1-12.7
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• 2011

Objectives: Perchlorate is an emerging contaminant that is found everywhere, including various foods. Perchlorate is known to disturb the production of thyroid hormones and leads to mental disorders in fetuses and infants, as well as metabolic problems in adults. In this study, we attempted to establish an LC-MS/MS method for measuring perchlorate in dairy products and used this developed method to investigate perchlorate levels in Korean milk and yogurt samples. Methods: The developed method of perchlorate analysis requires a shaker and 1% acetic acid/acetonitrile as the extracting solvent. Briefly, the samples were extracted and then centrifuged (4000 rpm, 1hour), and the supernatant was then passed through a $Envi^{TM}$ Carb SPE cartridge that had been prewashed sequentially with 6 mL of acetonitrile and 6 mL of 1% acetic acid in water. The final volume of the sample extract was adjusted to 40 mL with reagent water and the final sample was filtered through a 0.20-${\mu}m$ pore size PTFE (Polytetrafluoroethylene) syringe filter prior to LC-MS/MS. Results: The average levels of perchlorate in milk and yogurt samples were $5.63{\pm}3.49\;{\mu}g/L$ and $3.65{\pm}2.42\;{\mu}g/L$, respectively. The perchlorate levels observed in milk samples in this study were similar to those reported from China, Japan, and the United States. Conclusions: The exposure of Koreans to perchlorate through the consumption of dairy products was calculated based on the results of this study. For all age groups, the calculated exposure to perchlorate was below the reference of dose (0.7 ${\mu}g/kg$-day) proposed by the National Academy of Science, USA, but the perchlorate exposure of children was higher than that of adults. Therefore, further investigation of perchlorate in other food samples is needed to enable a more exact assessment of exposure of children to perchlorate.

• Parasites, Hosts and Diseases
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• v.51 no.1
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• pp.93-98
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• 2013

A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.587-595
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• 2019

Pharmacological research on (CHA), a marine-derived quinazolinone alkaloid with significant cytotoxic activity, is restricted by low yields and is a problem that needs to be settled urgently. In this work, the selection of additional nitrogen sources and the optimization of additional concentrations and longer fermentation times using ammonium acetate, were investigated. CHA production was optimized to 62.1 mg/l with the addition of 50 mM ammonium acetate at 120 h of the fermentation in the shaker flask. This feeding strategy significantly increased 3-deoxy-arabino-heptulosonate-7-phosphate synthase activity and transcript levels of critical genes (laeA, dahp, and trpC) in the shikimate pathway compared with the non-treatment group. In addition, the selection of the feeding rate (0.01 and $0.03g/l{\cdot}h$) was investigated in a 5-L bioreactor. As a result, CHA production was increased by 57.9 mg/l with a $0.01g/l{\cdot}h$ ammonium acetate feeding rate. This work shows that the strategy of ammonium acetate supplementation had an effective role in improving CHA production by Aspergillus fumigatus CY018. It also shows that this strategy could serve as an important example of large-scale fermentation of a marine fungus in submerged culture.

• The Journal of the Acoustical Society of Korea
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• v.38 no.2
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• pp.193-199
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• 2019

In this paper, the results of underwater vibration experiment are analyzed to verify platform vibration-induced noise isolation characteristics of a hull-mounted acoustic sensor. The experimental condition causing platform vibration-induced noise is generated using the mock-up hull, where the acoustic sensor is installed, with shaker in an acoustic water tank. The performance indices of ATF (Acceleration Transfer Function), AVS (Acceleration Voltage Sensitivity), and IL (Insertion Loss) for the acoustic sensor are calculated from the output of the standard accelerometers, which are installed on the mock-up hull and the acoustic sensor, and the output signal of the acoustic sensor. The frequency-dependent noise isolation characteristics of the acoustic sensor are analyzed based on the calculated performance indices and an effectiveness of the experiment is examined.

• BMB Reports
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• v.54 no.10
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• pp.497-504
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• 2021

EGR1 (early growth response 1) is dysregulated in many cancers and exhibits both tumor suppressor and promoter activities, making it an appealing target for cancer therapy. Here, we used a systematic multi-omics analysis to review the expression of EGR1 and its role in regulating clinical outcomes in breast cancer (BC). EGR1 expression, its promoter methylation, and protein expression pattern were assessed using various publicly available tools. COSMIC-based somatic mutations and cBioPortal-based copy number alterations were analyzed, and the prognostic roles of EGR1 in BC were determined using Prognoscan and Kaplan-Meier Plotter. We also used bc-GenEx-Miner to investigate the EGR1 co-expression profile. EGR1 was more often downregulated in BC tissues than in normal breast tissue, and its knockdown was positively correlated with poor survival. Low EGR1 expression levels were also associated with increased risk of ER+, PR+, and HER2- BCs. High positive correlations were observed among EGR1, DUSP1, FOS, FOSB, CYR61, and JUN mRNA expression in BC tissue. This systematic review suggested that EGR1 expression may serve as a prognostic marker for BC patients and that clinicopathological parameters influence its prognostic utility. In addition to EGR1, DUSP1, FOS, FOSB, CYR61, and JUN can jointly be considered prognostic indicators for BC.

• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1430-1437
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• 2021

Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD₅₄₀ = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.