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http://dx.doi.org/10.3347/kjp.2013.51.1.93

Monoclonal Antibody-Based Dipstick Assay: A Reliable Field Applicable Technique for Diagnosis of Schistosoma mansoni Infection Using Human Serum and Urine Samples  

Demerdash, Zeinab (Immunology Department, Theodor Bilharz Research Institute)
Mohamed, Salwa (Immunology Department, Theodor Bilharz Research Institute)
Hendawy, Mohamed (Parasitology Department, Theodor Bilharz Research Institute)
Rabia, Ibrahim (Parasitology Department, Theodor Bilharz Research Institute)
Attia, Mohy (Gastroentrology & Endoscopy Department, Theodor Bilharz Research Institute)
Shaker, Zeinab (Immunology Department, Theodor Bilharz Research Institute)
Diab, Tarek M. (Parasitology Department, Theodor Bilharz Research Institute)
Publication Information
Parasites, Hosts and Diseases / v.51, no.1, 2013 , pp. 93-98 More about this Journal
Abstract
A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.
Keywords
Schistosoma mansoni; monoclonal antibody; dipstick assay; sandwich ELISA;
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1 Katz N, Coelho PMZ. Clinical therapy of schistosomiasis mansoni: the Brazilian contribution. Acta Trop 2008; 108: 72-78.   DOI   ScienceOn
2 Montresor A, Crompton DW, Gyorkos TW, Savioli L. Helminth control in school age children: a guide for managers of control programmes. Geneva, Switzerland. World Health Organization. 2002.
3 WHO. Report of the WHO informal consultation on schistosomiasis in low transmission areas: control strategies and criteria for elimination. WHO/CDS/CPE/SIP/2001.1. London, UK. World Health Organization. 2000.
4 WHO. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO Expert Committee. Geneva, Switzerland. World Health Organization. WHO Tech Rep Series 2002; Vol. 912.
5 Qian ZL, Deelder AM. Schistosoma japonicum: immunological response to circulating polysaccharide antigens in rabbits with a light infection. Exp Parasitol 1983; 55: 394-403.   DOI   ScienceOn
6 Shaker ZA, Kaddah MA, Hanallah SB, El-Khodary MI. Production of monoclonal antibodies against target schistosomal antigen secreted in the urine of Schistosoma mansoni-infected patients. Int J Parasitol 1998; 28: 1893-1901.   DOI   ScienceOn
7 Hanallah SB, Mohamed SH, Maher KM, Amer NM, Sayed HA, Demerdash ZA, Shaker ZA. A monoclonal antibody against tegument of adult S. mansoni worms detects active schistosomal infection. Kasr El Aini Med J 2003; 9: 209-223.
8 Salah F, El Bassiouny A, Rabie I, Demerdash Z, Roshdy M, Shaker Z. Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen. Parasitol Res 2006; 99: 528-533.   DOI
9 Mahfouz AM, Rabee I, El Amir A. Evaluation of different immunological techniques for diagnosis of schistosomiasis haematobium in Egypt. Biotechnology 2012; 11: 10-19.   DOI
10 Hendawy MA, Mohamed SH, Ibrahim RA. Nitrocellulose cassettes (NCC) as a field applicable technique for rapid and effective diagnosis of schistosomiasis and fascioliasis. New Egypt J Med 2006; 35: 86-92.
11 Ibrahim RB, Mohamed SH, Demerdash ZA, Diab TM, Maher K, Safwat W, Shaker ZA. Anti-S. mansoni mAb-based latex agglutination: a reliable field applicable immunodiagnostic test for screening of active human schistosomiasis. J Am Sci 2010; 6: 19-27.
12 Gezahegan T. Development of a dipstick method for mosquito blood-meal identification and comparison with ELISA for potential field use. Med Sei 1989; 42: 593-600.
13 Stott DI. Immunoblotting and dot blotting. J Immunol Methods 1989; 119: 153-187.   DOI   ScienceOn
14 Van Dam GJ, Wichers JH, Ferreira TM, Ghati D, van Amerongen A, Deelder AM. Diagnosis of schistosomiasis by reagent strip test for detection of circulating cathodic antigen. J Clin Microbiol 2004; 42: 5458-5461.   DOI   ScienceOn
15 Stothard JR, Kabatereine NB, Tukahebwa EM, Kazibwe F, Rollinson D, Mathieson W, Webster JP, Fenwick A. Use of circulating cathodic antigen (CCA) dipsticks for detection of intestinal and urinary schistosomiasis. Acta Trop 2006; 97: 219-228.   DOI   ScienceOn
16 Deelder AM, De Jonge N, Boerman OC, Fillie YE, Hilberath GW, Rotmans JP, Gerritse MJ, Schut DW. Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies. Am J Trop Med Hyg 1989; 40: 268-272.
17 Van Lieshout L, De Jonge N, Mansour MM, Bassily S, Krijger FW, Deelder AM. Circulating cathodic antigen levels in serum and urine of schistosomiasis patients before and after chemotherapy with praziquantel. Trans R Soc Trop Med Hyg 1993; 87: 311-312.   DOI   ScienceOn
18 Mahmoud S, Demerdash ZA, Mohamed SH, Badawy AA, Mohamed SH, Shaker ZA, Ibrahim AM. Study of kinetics of circulating schistosome antigens in tissue and sera of infected mice using anti-Schistosoma mansoni soluble egg antigen monoclonal antibody. Egypt J Schistoso Infect Endem Dis 2002; 24: 1-17.
19 Bosompem KM, Ayi I, Anyan WK, Nkrumah FK, Kojima S. Limited field evaluation of a rapid monoclonal antibody-based dipstick assay for urinary schistosomiasis. Hybridoma 1996; 15: 443-447.   DOI   ScienceOn
20 Nibbeling HA, Van Lieshout L, Deelder AM. Levels of circulating soluble egg antigen in urine of individuals infected with Schistosoma mansoni before and after treatment with praziquantel. Trans R Soc Trop Med Hyg 1998; 92: 675-677.   DOI   ScienceOn
21 Cai YC, Guo J, Chen SH, Tian LG, Steinmann P, Chen MX, Li H, Ai L, Chen JX. Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum. Parasitol Int 2012; 61: 385-390.   DOI   ScienceOn
22 Emukah E, Gutman J, Eguagie J, Miri ES, Yinkore P, Okocha N, Jibunor V, Nebe O, Nwoye AI, Richards FO. Heme dipsticks are useful in monitoring the impact of praziquantel treatment on Schistosoma haematobium in sentinel communities of Delta State, Nigeria. Acta Trop 2012; 122: 126-131.   DOI   ScienceOn
23 Peters PA, Mahmoud AA, Warren KS, Ouma JH, Arap Siongok TK. Field studies of a rapid, accurate means of quantifying Schistosoma hematobium eggs in urine samples. Bull WHO 1976; 54: 159-162.
24 Blagg W, Mansour NS, Khalaf GL. A new concentration technique for the demonstration of protozoa and helminthic eggs in feces. Am J Trop Med Hyg 1955; 4: 23.
25 Mohamed S, Diab TM, Safwat W, Demerdash Z, Shaker Z. An effective anti-S. mansoni monoclonal antibody for detection of human antigenaemia and antigenurea. Egypt J Med Sci 2007; 12: 25-33.
26 Siongok TK, Mahmoud AA, Ouma JH, Warren KS, Muller AS, Handa AK, Houser HB. Morbidity in schistosomiasis mansoni in relation to intensity of infection: study of a community in Machakos, Kenya. Am J Trop Med Hyg 1976; 25: 273-284.
27 Oaks JA, Cain A, Mower DA, Raj RK. Distribution and removal of the tegument from Schistosoma mansoni with triton X-100. J Parasitol 1981; 67: 761-774.   DOI   ScienceOn
28 Engvall E, Perlman P. Enzyme-linked immunosorbent assay (EL1SA). Quantitative assay of immunuglobulin G. J Immunochem 1971; 8: 871-874.   DOI   ScienceOn
29 Galfre G, Milstein C. Properties of monoclonal antibodies: strategies and procedures. Meth Enzymol 1981; 73: 3.   DOI
30 Nakane PK, Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem 1974; 22: 1084-1091.   DOI   ScienceOn
31 van Etten L, Folman CC, Eggelte TA, Kremsner PG, Deelder AM. Rapid diagnosis of schistosomiasis by antigen detection in urine with a reagent strip. J Clin Microbiol 1994; 32: 2404-2406.
32 Galen RS. Predictive values and efficiency of laboratory testing. Pediat J Clin North Am 1980; 27: 861-869.
33 WHO. Gearing up to eliminate schistosomiasis with increased donation of praziquantel from Merck KGaA. Geneva, Switzerland. World Health Organization. 2012.
34 Utzinger J, Raso G, Brooker S, De Savigny D, Tanner M, Ornbjerg N, Singer BH, N'goran EK. Schistosomiasis and neglected tropical diseases: towards integrated and sustainable control and a word of caution. Parasitology 2009; 136: 1859-1874.   DOI   ScienceOn
35 Allam AF. Diagnosis of schistosomiasis in low endemic areas. In Tech Open Book 2012; 1: 1-10 (www.intechopen.com/download/pdf/pdfs).
36 Salah F, Demerdash Z, Shaker Z, El Bassiouny A, El Attar G, Ismail S, Abadir N, Saad El Din A, Mansour M. A monoclonal antibody against Schistosoma haematobium soluble egg antigen(s): efficacy for diagnosis and monitoring of cure of Schistosoma haematobium infection. Parasitol Res 2000; 86: 74-80.   DOI
37 Stothard JR, Sousa-Figueiredo JC, Standley C, Van Dam GJ, Knopp S, Utzinger J, Ameri H, Khamis AN, Khamis IS, Deelder AM, Mohammed KA, Rollinson D. An evaluation of urine-CCA strip test and fingerprick blood SEA-ELISA for detection of urinary schistosomiasis in schoolchildren in Zanzibar. Acta Trop 2009; 111: 64-70.   DOI   ScienceOn
38 Bosompem KM, Owusu O, Okanla EO, Kojima S. Applicability of a monoclonal antibody-based dipstick in diagnosis of urinary schistosomiasis in the Central Region of Ghana. Trop Med Int Health 2004; 9: 991-996.   DOI   ScienceOn
39 Bosompem KM, Ayi I, Anyan WK, Arishima T, Nkrumah FK, Kojima S. A monoclonal antibody-based dipstick assay for diagnosis of urinary schistosomiasis. Trans R Soc Trop Med Hyg 1997; 91: 554-556.   DOI   ScienceOn
40 Ashton RA, Stewart BT, Petty N, Lado M, Finn T, Brooker S, Kolaczinski JH. Accuracy of circulating cathodic antigen tests for rapid mapping of Schistosoma mansoni and S. haematobium infections in Southern Sudan. Trop Med Int Health 2011; 16: 1099-1103.   DOI   ScienceOn