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Biodegradation Characteristics of Poly-3-hydroxybutyrate, $Sky-Green^R$ and $Mater-Bi^R$ by Soil Bacteria (토양세균의 Poly-3-hydroxybutyrate,$Sky-Green^R$$Mater-Bi^R$분해 특성)

  • 이애리;김말남
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.299-305
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    • 2000
  • Degradation behavior of the three commercial biodegradable polymers, namely poly(3-hydroxybutyrate) (PHB) Sky-Green/sup R/ (SG) and Mater-Bi/sup R/ (MB) was investigated using bacteria isolated from activated sludge and farm soil. Three PHB degrading bacteria, three SG degrading bacteria and one MB degrading bacteria were isolated. The PHB degrading bacteria were identified to be Flavimonas oryzihabitans, Corynebacterium pseudodiphtheriticum and Micrococcus diversus, while Pseudomonas vesicuraris, Pasteurlla multocida and Flavobacterium odoratum were identified as SG degrading bacteria. As for MB, Pseudomonas vesicuraris was isolated. The shake flask test for 28 days indicated that the rate of biodegradation of PHB, SG and MB in terms of weight loss were about 44∼69% 25∼32% and 29% respectively. The surface morphology of PHB, SG andMB films before and after degradation by microorganisms in an activated sludge soil was observed under SEM, demonstrating that the film surface had a very porous structure, and that microorganisms colonized heavily on the film surface. TOC and pH variation as a result of abiotic hydrolysis, or microbial growth in the absence of the polymers were compared to those due to degradation by F. oryzihabitans. Abiotic hydrolysis of PHB was three times as fast as that of SG and MB. Addition of yeast extract to the basal liquid medium accelerated the biodegradation of the polymers. Biodegradation of PHB was always faster than that of SG and MB irrespectively of the presence of yeast extract in the basal liquid medium.

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People within the Forest, People outside the Forest : A View from Ecological Anthropology (숲속에 사는 사람, 숲밖에 사는 사람 : 생태인류학적(生態人類學的) 관점(觀點))

  • Chun, Kyung Soo
    • Journal of Korean Society of Forest Science
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    • v.79 no.3
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    • pp.330-342
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    • 1990
  • One might have a retrospect on the relationship between the forest and human being from the viewpoint of ecological perspective. It is no doubt that most of the fossil humans should have lived on the forest and the latter provided foods and shelters for humans from their beginning stages, Since the so-called agricultural revolution, humans have extensively started to exploit the forest which had beer, their cradle. The industrial revolution has created another situation against the forest in terms of the quality of ecosystem. These two revolutions have set up the so-called civilization which seems to have been based on the sacrificial oblation of the forest. The cradle for human being has been kept exterminating for the shake of "economic development and miracle." This might be a synoptic history of relationships between the forest and human beings in a sense. designates the behavioral aspects of human being against the forest and people consider the forest only as exploitable resource in this context, and the latter means that people live on the forest and strive to adapt the order of forest ecosystem. The resourcism has developed a strategy of colonialism to exploit the forest and provided a winner's position for the human beings against the forest, This idea and behavioral perspective seems to have started the backfire against the exploiter who is the owner of the civilization. However, there are different philosophies and ideas to view the relationship between the forest and human beings. People within the forest who are mostly considered as "primitives" still keep their idea of the ontology of the forest. There is a theoretical assumption of the "socionatural system" to look into the ecosystem. The forest could be viewed in the above frame of analysis. There are five variables : environment, resource, technology, organization, and ideology. Ideological aspect of the forest can be explained in the context of belief systems. Forest has a meaning of religion and rituals and people within the forest should admire it in anyway of religious reasons. This aspect of the forest cannot be separated from the environmental aspect of the forest. People within the forest acknowledge and practice the above idea. People outside the forest have lost the idea, however, at the cost of acquiring the civilization. They have expelled themselves from the forest and divided the socionatural system of the forest by way of colonialism. The efforts like agroforestry and social forestry would be strategies for recovering the idea of ontology of the forest as well as the sense of community including the forest and human being. People within the forest will be a prospective model for the future socionatural system of the forest for the people outside the forest. At this point, an ecological anthropologist can work with the forest specialists.

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Development of Osmotolerant Mutant, Candida magnoliae M26 and the Determination of the Optimum Concentrations of Carbon and Nitrogen Sources to Improve Erythritol Yield (에리스리톨의 수율 향상을 위한 Candida magnoliae의 삼투압 내성 변이균주의 개발과 탄소원 및 질소원의 최적 농도 결정)

  • 양성욱;서진호;유연우
    • KSBB Journal
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    • v.15 no.6
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    • pp.566-572
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    • 2000
  • Experimental studies were carried out to develop an osmotolerant mutant of Candida magnoliae JH and to determine the optimum concentrations of carbon and nitrogen sources to improve erythritol yield and productivity. Mutants of C. magnoliae JH were prepared by treatment with ethylmethane sulfonate, and osmotolerant mutants were isolated from the agar plate colonies containing 2.5 M KCI. Among the mutants isolated, one mutant M26 was finally selected based on erythritol yield and productivity. In shake flask culture, glucose proved to be the best carbon source and the optimum yeast extract concentration was 5 g/L based on 100 g/L glucose. The erythritol yield and productivity of mutant M26 were greater than wild type in 100 g/L glucose medium. in the fermentation experiments, erythritol production increased with increased glucose concentration, up to a limit of 250 g/L. The maximum concentration of erythritol achieved 127.5 g/L, and the yield and productivity of erythritol production were 51.0% and 0.63 g/L-h, respectively.

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Total reference-free displacements for condition assessment of timber railroad bridges using tilt

  • Ozdagli, Ali I.;Gomez, Jose A.;Moreu, Fernando
    • Smart Structures and Systems
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    • v.20 no.5
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    • pp.549-562
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    • 2017
  • The US railroad network carries 40% of the nation's total freight. Railroad bridges are the most critical part of the network infrastructure and, therefore, must be properly maintained for the operational safety. Railroad managers inspect bridges by measuring displacements under train crossing events to assess their structural condition and prioritize bridge management and safety decisions accordingly. The displacement of a railroad bridge under train crossings is one parameter of interest to railroad bridge owners, as it quantifies a bridge's ability to perform safely and addresses its serviceability. Railroad bridges with poor track conditions will have amplified displacements under heavy loads due to impacts between the wheels and rail joints. Under these circumstances, vehicle-track-bridge interactions could cause excessive bridge displacements, and hence, unsafe train crossings. If displacements during train crossings could be measured objectively, owners could repair or replace less safe bridges first. However, data on bridge displacements is difficult to collect in the field as a fixed point of reference is required for measurement. Accelerations can be used to estimate dynamic displacements, but to date, the pseudo-static displacements cannot be measured using reference-free sensors. This study proposes a method to estimate total transverse displacements of a railroad bridge under live train loads using acceleration and tilt data at the top of the exterior pile bent of a standard timber trestle, where train derailment due to excessive lateral movement is the main concern. Researchers used real bridge transverse displacement data under train traffic from varying bridge serviceability levels. This study explores the design of a new bridge deck-pier experimental model that simulates the vibrations of railroad bridges under traffic using a shake table for the input of train crossing data collected from the field into a laboratory model of a standard timber railroad pile bent. Reference-free sensors measured both the inclination angle and accelerations of the pile cap. Various readings are used to estimate the total displacements of the bridge using data filtering. The estimated displacements are then compared to the true responses of the model measured with displacement sensors. An average peak error of 10% and a root mean square error average of 5% resulted, concluding that this method can cost-effectively measure the total displacement of railroad bridges without a fixed reference.

The Vibration Comfort Evaluation of the Shaking Table Mass Foundation (진동대 반력기초의 진동사용성 평가)

  • Choi, Hyoung-Suk;Jung, Da-Jung;Kim, Seong-Do;Cheung, Jin-Hwan
    • Journal of the Earthquake Engineering Society of Korea
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    • v.15 no.2
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    • pp.53-60
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    • 2011
  • When designing building structures, dynamic serviceability is one of the most important items. Much research is being carried out on machine vibrations that affect inside residents and expensive equipment in the building structure. The vibration effect generally depends on the mass ratio, and an adequate mass ratio is determined by comparison with the serviceability limit according to the criteria. This study investigates the evaluation of vibration serviceability by using ISO 2631 to confirm the propriety of adequate mass ratios and it is verified that the application of a complicated FE model to model the real large shaking table facility with the mathematical model simulated as a SDOF system. The weighted RMS value is then compared with the comfort limit given by ISO 2631. As a result, the analysis of the numerical model is consistent with analysis of the FE model. Moreover, it is found that the adequate mass ratio of the concrete foundation and shake table, considering the self-weight of the real facility, should be less than 0.013. It is also confirm that the sample facility is satisfies the requirement of an adequate mass ratio.

Isolation and Identification of Lipolytic Enzyme Producing Pseudomonas sp. OME and Optimization of Cultural Conditions (지방분해효소 생산균 Pseudomonas sp. OME 의 분리 동정 및 배양조건 최적화)

  • Kumar, G.Satheesh;Reddy, T. Kiran;Madhavi, B.;Teja, P.Charan;Chandra, M.Subhosh;Choi, Yong-Lark
    • Journal of Life Science
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    • v.20 no.5
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    • pp.662-669
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    • 2010
  • Lipolytic enzyme-producing bacteria were isolated from edible oil mill effluents on tributyrin agar medium. The shake-flask-scale studies yielded a promising isolate and it was identified as Pseudomonas sp. An OME using various microbiological observations such as cultural, microscopic, and biochemical tests was undertaken and confirmed using PIBWIN bacterial identification software. Lipolytic enzyme production was screened with oils such as sunflower, caster, coconut, tributyrin, and olive. Amongst these, olive oil showed an increased lipase production 6.1 U/ml. In view of the highest lipolytic enzyme production with olive oil, further optimizations were carried out using olive oil as a carbon source. Lipolytic enzyme production was optimized by a conventional 'one variable at a time' approach and the significant factors were further analyzed statistically using response surface methodology (RSM). The effect of physical factors such as incubation time, temperature, initial medium pH, and nutritional factors such as concentration of olive oil and yeast extract were examined for lipase production. Lipolytic enzyme secretion was strongly affected by three variables (incubation time, concentration of yeast extract and olive oil). Therefore, the interaction of these three factors was further optimized using response surface methodology. The optimized conditions of lipase production using response surface methodology yielded a maximum of 9.62 U/ml with optimum conditions for incubation, yeast extract and olive oil concentrations were found to be 48 hr, 0.3 g. and 0.9 ml. respectively.

A Deep Learning-based Real-time Deblurring Algorithm on HD Resolution (HD 해상도에서 실시간 구동이 가능한 딥러닝 기반 블러 제거 알고리즘)

  • Shim, Kyujin;Ko, Kangwook;Yoon, Sungjoon;Ha, Namkoo;Lee, Minseok;Jang, Hyunsung;Kwon, Kuyong;Kim, Eunjoon;Kim, Changick
    • Journal of Broadcast Engineering
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    • v.27 no.1
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    • pp.3-12
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    • 2022
  • Image deblurring aims to remove image blur, which can be generated while shooting the pictures by the movement of objects, camera shake, blurring of focus, and so forth. With the rise in popularity of smartphones, it is common to carry portable digital cameras daily, so image deblurring techniques have become more significant recently. Originally, image deblurring techniques have been studied using traditional optimization techniques. Then with the recent attention on deep learning, deblurring methods based on convolutional neural networks have been actively proposed. However, most of them have been developed while focusing on better performance. Therefore, it is not easy to use in real situations due to the speed of their algorithms. To tackle this problem, we propose a novel deep learning-based deblurring algorithm that can be operated in real-time on HD resolution. In addition, we improved the training and inference process and could increase the performance of our model without any significant effect on the speed and the speed without any significant effect on the performance. As a result, our algorithm achieves real-time performance by processing 33.74 frames per second at 1280×720 resolution. Furthermore, it shows excellent performance compared to its speed with a PSNR of 29.78 and SSIM of 0.9287 with the GoPro dataset.

Understanding the Occurrence of Lung Cancer in Foundry Workers through Health Insurance Data (의료보험 전산자료 주상병명으로 파악한 주물공장 근로자들의 폐암)

  • Song, Jae-Seok;Kang, Seong-Kyu;Chung, Ho-Keun;Ahn, Yeon-Soon
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.3
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    • pp.299-305
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    • 2000
  • Objectives : To investigate the difference in the occurrence of lung cancer between foundry workers and non-foundry workers by comparing the number of workers diagnosed with lung cancer through health insurance data. Methods : The study population was comprised of 28,884 workers who had undergone at least one general or special medical examination between January 1995 and December 1997 at the occupational health center. All of the subjects had health insurance during this period. We combined the medical examination data with the health insurance data to compare the number of foundry workers diagnosed with lung cancer and the number of non-foundry workers diagnosed with lung cancer. Results : Seven workers were diagnosed with lung cancer among the 1,591 foundry workers, compared to twelve workers among the 27,293 non-foundry workers (odds ratio: 10.04, 95% confidence interval: 3.95-25.55). The seven foundry workers diagnosed with lung cancer were all exposed to dust, and six out these seven workers were engaged in finishing or shake-out processes. Conclusions : Although the information for this study was obtained from health insurance data, which has limitations such as accuracy and completeness, the number of foundry workers diagnosed with lung cancer was significantly higher than that of non-foundry workers. Therefore, a well-designed cohort study should be followed to confirm the higher lung cancer rates in foundry workers.

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Earthquake Monitoring : Future Strategy (지진관측 : 미래 발전 전략)

  • Chi, Heon-Cheol;Park, Jung-Ho;Kim, Geun-Young;Shin, Jin-Soo;Shin, In-Cheul;Lim, In-Seub;Jeong, Byung-Sun;Sheen, Dong-Hoon
    • Geophysics and Geophysical Exploration
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    • v.13 no.3
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    • pp.268-276
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    • 2010
  • Earthquake Hazard Mitigation Law was activated into force on March 2009. By the law, the obligation to monitor the effect of earthquake on the facilities was extended to many organizations such as gas company and local governments. Based on the estimation of National Emergency Management Agency (NEMA), the number of free-surface acceleration stations would be expanded to more than 400. The advent of internet protocol and the more simplified operation have allowed the quick and easy installation of seismic stations. In addition, the dynamic range of seismic instruments has been continuously improved enough to evaluate damage intensity and to alert alarm directly for earthquake hazard mitigation. For direct visualization of damage intensity and area, Real Time Intensity COlor Mapping (RTICOM) is explained in detail. RTICOM would be used to retrieve the essential information for damage evaluation, Peak Ground Acceleration (PGA). Destructive earthquake damage is usually due to surface waves which just follow S wave. The peak amplitude of surface wave would be pre-estimated from the amplitude and frequency content of first arrival P wave. Earthquake Early Warning (EEW) system is conventionally defined to estimate local magnitude from P wave. The status of EEW is reviewed and the application of EEW to Odesan earthquake is exampled with ShakeMap in order to make clear its appearance. In the sense of rapidity, the earthquake announcement of Korea Meteorological Agency (KMA) might be dramatically improved by the adaption of EEW. In order to realize hazard mitigation, EEW should be applied to the local crucial facilities such as nuclear power plants and fragile semi-conduct plant. The distributed EEW is introduced with the application example of Uljin earthquake. Not only Nation-wide but also locally distributed EEW applications, all relevant information is needed to be shared in real time. The plan of extension of Korea Integrated Seismic System (KISS) is briefly explained in order to future cooperation of data sharing and utilization.

Studies on the Production of Intra- and Extra-cellular Lipids by the Strains in the Genus RHODOTORULA (Rhodotorula 속(屬) 균주(菌株)에 의(依)한 세포(細胞) 내외(內外) 지질생산(脂質生産)에 관(關)한 연구(硏究))

  • Park, Sung-Oh
    • Applied Biological Chemistry
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    • v.17 no.2
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    • pp.93-116
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    • 1974
  • A potent intracellular-lipid-producing yeast, Rhodotorula glutinis var. glutinis SW-17, was screened out from a variety of arable soils, compost heaps, and fodders, and two strains of excellent extracellular-lipid-producing yeasts, Rhodotorula glutinis var. glutinis SW-5 and Rhodotorula graminis SW-54, were screened out from the surface of many species of leaves. And then the intra- and extra-cellular lipid productions by those Rhodotorula yeasts were studied. The results were as follows: 1. During the shaking culture of 8 days at $24^{\circ}C$, both the intra- and extra-cellular lipid accumulation started almost at the stationary phase of growth, when the nitrogen source in the medium was a little more than half used up. The intracellular lipid production by Rhodotorula glutinis var. glutinis SW-17 reached 58.42% (w/w) of dried yeast, and the extracellular lipid production by Rhodotorula graminis SW-54 amounted to 2.62g per liter of the medium. 2. After the carbon and nitrogen sources in the medium were almost consumed, if the yeasts were shake-cultured further in a state of starvation, the yeast cells re-utilized the already produced intra- and extra-cellular lipids and the lipids completely disappeared in the medium in about 90 days. 3. The relative concentration of carbon and nitrogen sources in the media greatly influenced both the intra- and extra-cellular lipid production. When the nitrogen source in the medium was almost used up for the growth of yeast, and excess carbon sources were still available, the lipid production vigorously proceeded. As long as the nitrogen source concentration in the medium was high, the lipid production was greatly suppressed. 4. The optimum pH for both the intra- and extra-cellular lipid production by those yeasts was pH 5.0-6.0. 5. The fatty acid components of the intracellular lipid of Rhodotorula glutinis var. glutinis SW-17 were myristic, palmitic, palmitoleic, stearic, oleic, linoleic, and linolenic acids. The largest components of the fatty acids were palmitic acid equivalent to 30-45% of the whole fatty acids and oleic acid equivalent to 35-50%. 6. The fatty acid components of the extracellular lipid of Rhodotorula glutinis var. glutinis SW-5 and Rhodotorula graminis SW-54 were myristic, palmitic, stearic, oleic, linoleic, linolenic, 3-D-hydroxypalmitic, and 3-D-hydroxystearic acids. The largest components of the fatty acids were 3-D-hydroxypalmitic acid equivalent to 22-25% of the acids and 3-D-hydroxystearic acid equivalent to 13-17%. 7. The polyol component of the intracellular lipids was only glycerol, whereas the polyols of extracellular lipids were glycerol, mannitol, xylitol and arabitol.

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