Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Biodegradation Characteristics of Poly-3-hydroxybutyrate, $Sky-Green^R$ and $Mater-Bi^R$ by Soil Bacteria

토양세균의 Poly-3-hydroxybutyrate,$Sky-Green^R$및$Mater-Bi^R$분해 특성

  • 이애리 (상명대학교 자연과학대학 생물학과) ;
  • 김말남 (상명대학교 자연과학대학 생물학과)
  • Published : 2000.12.01
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Abstract

Degradation behavior of the three commercial biodegradable polymers, namely poly(3-hydroxybutyrate) (PHB) Sky-Green/sup R/ (SG) and Mater-Bi/sup R/ (MB) was investigated using bacteria isolated from activated sludge and farm soil. Three PHB degrading bacteria, three SG degrading bacteria and one MB degrading bacteria were isolated. The PHB degrading bacteria were identified to be Flavimonas oryzihabitans, Corynebacterium pseudodiphtheriticum and Micrococcus diversus, while Pseudomonas vesicuraris, Pasteurlla multocida and Flavobacterium odoratum were identified as SG degrading bacteria. As for MB, Pseudomonas vesicuraris was isolated. The shake flask test for 28 days indicated that the rate of biodegradation of PHB, SG and MB in terms of weight loss were about 44∼69% 25∼32% and 29% respectively. The surface morphology of PHB, SG andMB films before and after degradation by microorganisms in an activated sludge soil was observed under SEM, demonstrating that the film surface had a very porous structure, and that microorganisms colonized heavily on the film surface. TOC and pH variation as a result of abiotic hydrolysis, or microbial growth in the absence of the polymers were compared to those due to degradation by F. oryzihabitans. Abiotic hydrolysis of PHB was three times as fast as that of SG and MB. Addition of yeast extract to the basal liquid medium accelerated the biodegradation of the polymers. Biodegradation of PHB was always faster than that of SG and MB irrespectively of the presence of yeast extract in the basal liquid medium.

생분해성 플라스틱으로 현재 상업화에 가장 근접해 있는 poly-3-hydroxybutyrate (PHB), $Sky-Green^R$(SG) 및 $Mater-Bi^R$(MB)를 활성오니토 및 경작토에 매립하여 PHB 분해세균 3주, SG 분해세균 3주 및 MB 분해세균 1주를 분리하였다. PHB 분해세균은 Flavimonas oryzihabitans, Corynebacterium pseudodiphtheriticum 및 Micrococcus diversus로, SG 분해세균은 Pseudomonas vesicuraris, Pasteurlla multocida 및 Flavobacterium odoratum으로, MB 분해세균은 Pseudomonas vesicuraris로 동정되었다. 각 플라스틱 film을 함유한 무기염 액체배지에서 28일 간의 생분해성 실험 결과 평균 중량감소는 PHB와 SG은 각각 44~69% 및 25~32%, MB는 29%를 나타내어 PHB의 생분해성이 가장 우수하였다. 각 플라스틱 film의 활성오니토 매립 전과 후의 표면 변화를 조사한 결과 미생물이 다공성의 film 표면에 많은 colony를 형성하는 것이 SEM으로 관찰되었다. PHB, SG 및 MB의 무생물적 분해와 F. oryzihabitans에 의한 미생물학적 생분해를 TOC와 pH 변화로 측정하여 비교 분석하였을 때, SG과 MB는 분해 정도가 서로 유사하였으나 PHB는 SG는 MB에 비하여 dir 3배 더 빠르게 무생물적 분해가 일어났다. Yeast extract를 포함한 배지에서 PHB, GS 및 MB의 분해는 yeast extract를 포함하지 않은 배지에서 보다 훨씬 빠르게 안정되었으며, 두 경우 모두 PHB의 분해는 SG 및 MB에 비하여 더 빠르게 일어졌다.

Keywords

References

  1. J. M. S. Pure. Appl. Chem. A v.32 no.4 Biodegradation of polymers at temperatures up to 130℃ Antranikian, G.;A. Rudiger;F. Canganella;M. Klingeberg;A. Sunna
  2. Polym. Degrad. Stabil. v.62 Radiation crosslinked poly(butylene succinate) foam and its biodegradation Bahari, K.;H. Mitomo;T. Enjoji;F. Yoshii;K. Makuuchi
  3. FEMS Microbiolgy Letters v.117 Degradation of poly (3-hydroxybutyrate-co-hydroxyvalerate) by aerobic sewage sludge Briese, B. H.;D. Jendrossek;H. G. Schlegel
  4. J. Environ. Polym. Degrad. v.4 Evaluation of biodegradability of poly(ε-caprolactone)/Poly(ethylene terephthalate) blends Chiellini, E.;A. Corti;A. Giovannini;P. Narducci;A.M. Paparella;R. Solaro
  5. Bergey's manual of Determinative Bacteriology(9th ed.) Holt, J.
  6. Polym. Degrad. Stabil. v.67 The susceptibility of poly(ether) urethanes to enzymatic degradation after oxidative treatment Hsu, S. H.;T. D. Huang
  7. J. M. S. Pure Appl. Chem. v.A 32 Polymer waste manegement-biodegradation, Incineration and recycling Huang, S. J.
  8. Macromolecules v.30 Enzymatic degradation and adsortion on poly[(R)-3-hydroxybutyrate] single crystal with two types of extracellular PHB depolymerase from Comamonas acidovorans YM1609 and Alcaligenes faecalis T1 Itawa, T.;Y. Doi
  9. Appl. Micribiol. Biotechnol. v.39 Bacterial degradation of acrylic oligomers and polymers Kawai, F.
  10. J. Environ. Polym. Degrad. v.4 Bacterial degradation of a new polyester, polyethylene glycol-phthalate polyester Kawai, F.
  11. Kor. J. Biotech. Bioeng. v.9 Biodegradable characteristics of starch-filled polyethylene film by fungi Kim, J. H.;T. H. Park;D. M. Shin;S. H. Lee;G. Y. Han
  12. Kor. J. Mycol. v.23 Biodegradation of poly(s-hydroxybutyrate)by Penicillium pinophilum Kim, M. N.;E. J. Kang
  13. Kor. J. Environ. Biology v.17 Effect of Surface Roughness on Biodegradability of Poly(3-Hydrobutyrate) Kim, M. N.;A. R. Lee
  14. Eur. Polym. J. v.35 Biodegradability of Poly(3-Hydrobutyrate) blended with Poly (Ethylene-co-vinyl acetate) or Poly(Ethylene Oxide) Kim, M. N.;A. R. Lee;K. H. Lee;I. J. ChinlJ. S. Yoon
  15. Eur. Polym. J. v.36 Biodegradation of Biopol？, SkyGreen？ and Mater-Bi？ by Fung Isolated from soils Kim, M. N.;A. R. Lee;J. S. Yoon;I. J. Chin
  16. Appl. Environ. Microbiol. v.65 The roal of benzoate anaerobic degradation trephthalate Klee Rebezen;R. L. W. Hulshoffpol;G. Letinga
  17. Enzyme Microb. Technol. v.21 Flavobacterium odoratum lipase: Isolation and characterization Labuschagne, R. B.;A. Tonder;D. Litthauer
  18. Micron. v.24 A Study of biodegradation of starch-plastic films in soil using scanning electron microscopy Lopez-llorca, L. V.;M. F. Colom Valiente;C. Gascon
  19. FEMS Microbiology Letters v.141 Determination of the active site serine of poly(3-hydroxybutyrate) depolymerase of Pseudomonas lemoignei(PhaZ5) and of Alcaligenes faecalis Shinohe, T.;M. Nojiri;T. Saito;T. Stanislawski;D. Jendrossek
  20. FEMS Microbiology Letters v.154 Cloning of the gene for poly(3-hydroxybutyric acid) Shinomiya, M.;T. Iwata;K. Kasuya;Y. Doi
  21. Macromolecules v.29 Enzymatic degradation of bacterial poly(3-hydroxybutyrate) by depolymerase from Pseudomonas lemoignei Tomasi, G.;M. Scandola;B. H. Bries;D. Jendrossek
  22. Appl. Environ. Microbiol v.62 Screening of Microorganisms for biodegradation of poly(lactic acid) and lactic acid-containing polymers Torres, A.;S. M. Li;S. Roussos;M. Vert
  23. J. Environ. Polym. Degrad. v.4 Biodegradability, hydrolytic degradability, and builder performance in detergent formulations of partially dicarboxylate alginic acid Winursito, I.;S. Matsunmura
  24. J. Environ. Polym. Degrad. v.5 Biodegradation behavior and material properties of aliphatic/aromatic polyesters of commercial importance Witt, W.;R. J. Muller;W. D. Deckwer