• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.592-599
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• 2019

Ostrea denselamellosa and Eriocheir japonica samples were collected from the Seomjin River in 2019 as part of the "Research of Host-Associated Bacteria" research program. Almost 200 bacterial strains were isolated from the O. denselamellosa and E. japonica samples and subsequently identified by 16S rRNA gene sequencing. Among the bacterial isolates, ten strains possessed greater than 98.7% sequence similarity with published bacterial species that had not previously been recorded in Korea. These species were phylogenetically diverse, belonging to three phyla, four classes, seven orders, and eight genera. At the genus and class level, the previously unrecorded species belonged to Pseudoalteromonas, Aliivibrio, Rheinheimera, Leucothrix, and Shewanella of the class Gamma-proteobacteria, Olleya of the class Flavobacteriia, Algoriphagus of the class Cytophagia, and Lactococcus of the class Bacilli. The previously unrecorded species were further characterized by examining their Gram staining, colony and cell morphology, biochemical properties, and phylogenetic positions.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.183-197
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• 2021

Various freshwater ecosystems, such as streams, lakes and rivers, provide a dynamic habitat for fungi. In this study, we isolated several fungal strains from freshwater sediment and plant litter. The strains were identified using molecular phylogenetic analyses of rDNA and beta tubulin (TUB) sequences. Morphological characteristics of the fungi were also investigated using microscopy and culture characteristics of the fungi grown on several media. We identified seven species previously unrecorded in Korea, Nothophoma spiraeae, Westerdykella aquatica, W. aurantiaca, W. dispersa, Chrysosporium sanyaense, C.pseudomerdarium and Taeniolella phialosperma.

• Journal of Species Research
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• v.6 no.2
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• pp.163-170
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• 2017

As a part of the research program 'Survey of freshwater organisms and specimen collection', freshwater samples were collected from Lakes Soyang and Chungju in 2016. Hundreds of bacterial strains were isolated from the samples and were identified based on 16S rRNA gene sequences. Among the bacterial isolates, strains showing higher than 98.7% sequence similarity with validly published bacterial species not reported in Korea were selected as unrecorded bacterial species. Based on 16S rRNA gene sequence similarity, 17 strains were identified as unrecorded bacterial species in Korea. The 17 bacterial strains were phylogenetically diverse and belonged to four phyla, seven classes, 13 orders, 14 families, and 16 genera. At generic level, the unreported species were affiliated with Caulobacter, Paracoccus, and Mesorhizobium of the class Alphaproteobacteria, Deefgea, Undibacterium, Chitinimonas, Inhella, and Sphaerotilus of the class Betaproteobacteria, Vibrio and Cellvibrio of the class Gammaproteobacteria, Sanguibacter and Clavibacter of the phylum Actinobacteria, Lactococcus of the phylum Firmicutes, Deinococcus of the class Deinococci, and Chryseobacterium and Flavobacterium of the phylum Bacteroidetes. The unreported species were further characterized by examining Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic position. The detailed description of the 17 unreported species are also provided.

• Journal of Species Research
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• v.10 no.3
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• pp.217-226
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• 2021

During a project aiming to comprehensively investigate indigenous prokaryotic species in Korea, a total of 20 bacterial strains phylogenetically belonging to the the class Bacilli of the phylum Firmicutes were isolated from various environmental sources such as soil, air, tidal flat, sea water, grain, wetland, breast milk and healthy human urine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that 20 bacterial strains showed the high sequence similarities (≥98.7%) to the closest type strains and formed robust phylogenetic clades with closely related species of validly published names in the class Bacilli of the phylum Firmicutes. In the present study, we report 20 species of 13 genera of seven families of two orders of one class in the phylum Firmicutes, which have not been previously reported in Korea. Morphological, biochemical, and physiological characteristics, isolation sources, and NIBR deposit numbers of these unrecorded bacterial species are described in the species descriptions.

• Journal of Species Research
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• v.12 no.spc2
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• pp.7-14
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• 2023

Several genera belonging to the family Acetobacteraceae were generally considered to be acetic acid bacteria (AAB) which produce acetic acid from alcohols using an oxidation pathway. Some species of the family Acetobacteraceae have been of interest to the industry due to their capability to produce vinegar. In 2018-2020, several bacterial strains were isolated from plants, fruits and vinegar in various regions of the Republic of Korea. Based on the 16S rRNA gene sequences, seven species classified into the family Acetobacteraceae were found to be ones unrecorded in the Republic of Korea, including two Asaia species, one Gluconacetobacter species, three Gluconobacter species and one Komagataeibacter species. As a result, we report Asaia lannensis AF11C3 (=KACC 22050) isolated from plant Chrysanthemum zawadskii, Asaia platycodi AF15C2 (=KACC 22051) isolated from plant Isodon inflexus, Gluconacetobacter liquefaciens C23-3 (=KACC 22064) isolated from fruit of Pyrus pyrifolia, Gluconobacter cerinus BGF2-R2 (=KACC 22053) isolated from fruit of Prunus armeniaca, Gluconobacter kondonii FR39A4 (=KACC 22388) isolated from persimmon fruit, Gluconobacter thailandicus FR36C4 (=KACC 22057) isolated from fruit of Pseudocydonia sinensis and Komagataeibacter melaceti SPV-2 (=KACC 22058) isolated from potato vinegar.

• Mycobiology
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• v.30 no.3
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• pp.133-138
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• 2002

Through seven field surveys to the Kyeryoungsan National Park from July to December of the year 2001, 116 specimens of the Aphyllophorales were collected and taxonomically examined. All the specimens were identified to the species by observation of morphological and microscopic characters, along with old specimens from the Kyeryoungsan National Park of SFC herbarium. They amounted to 10 families, 37 genera and 52 species, and two species among them, Antrodia xantha and Ceriporia viridans, were confirmed as new to Korea and are described here as unrecorded species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.6
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• pp.965-967
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• 2020

Two shark species captured in Samcheok in 2006 and Ulsan in 2013, previously reported as Heptranchias perlo and Alopias pelagicus, were confirmed to in fact be Hexanchus griseus and A. superciliosus, respectively, neither of which has previously been reported in Korea. H. griseus has six gill slits and is thus distinguishable from Heptranchias perlo, which has seven gill slits. A. superciliosus is clearly distinguishable from A. pelagicus and A. vulpinus, members of the family Alopiidae, due to its large eyes and a groove extending from the center of the head to both eyes. The keys to the family Hexanchidae, including H. griseus, and the family Alopiidae, including A. superciliosus, were presented along with the morphological characteristics of these two new shark species. These two species were given the Korean names "Gi-reum-sang-eo" and "Keun-nun-hwan-do-sang-eo," with reference to their morphological characteristics and English names.

• Journal of Plant Biology
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• v.36 no.1
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• pp.9-17
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• 1993

Seven dictyostelid cellular slime molds isolated from the forest soils of Mt. Halla were described and illustrated. Dictyostelium flavidum Hong et Chang and three unidentified Dictyostelium, HL-2, HL-3 and CJ-9 were compared and discussed. All the isolates were cultivated on low nutrient media, 0.1- L-P(pH6) with Escherichia coli at 20-22$^{\circ}C$. Seven unrecorded species were all polar granule (PG) negative. These were Dictyostelium crassicaule Hagiwara, D. sphaerocephalum (Oud.) Sacc. et March, D. macrocephalum Hagiwara, Yeh et Chien, D. capitatum Hagiwara, D. brefeldianum Hagiwara, D. aureum var. aureum Cavender, Worley and Raper, and D. giganteum Singh.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.9-21
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• 2018

Seven unrecorded species in the phylum Ascomycota, Emericellopsis pallida (KNU16-167), Scedosporium aurantiacum (KNU16-190), Duddingtonia flagrans (KNU16-279), Bionectria rossmaniae (KNU16-309), Exophiala xenobiotica (KNU16-79), Pseudocercosporella fraxini (KNU16-102), and Stachybotrys sansevieriae (KNU16-141), were isolated in 2016 from field soils collected from various locations in Korea. All of the species were identified and described based on morphological characteristics and rDNA internal transcribed spacer sequence data. Morphological features of these fungi were examined on potato dextrose agar, oatmeal agar, malt extract agar, Czapek yeast extract agar, and yeast extract sucrose agar. Full descriptions and illustrations of their morphological characteristics are provided.

• Mycobiology
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• v.28 no.3
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• pp.142-148
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• 2000

To evaluate the establishment of Mungyong Saejae Natural Ecology Park located in the northwestern Gyongbuk Province, a scientific survey for the mushroom flora of the park was carried out from May to December of 1999. A checklist of the Aphyllophorales collected from the park was prepared. The list included 67 species of 44 genera belonging to nine families in the Aphyllophorales. Among them, seven species, Antrodia malicola, Ceriporia purpurea, Oligoporus leucospongia, Perenniporia tephropora, Phanerochaete xerophila, Sistotrema diademiferum and Vuilleminia comedens, were confirmed as new to Korea and are registered here as unrecorded species along with descriptions and microscopic drawings.