• 동의생리병리학회지
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• 제22권1호
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• pp.89-95
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• 2008

This experimental study was designed to investigate the effects of Ijin-tang add Atractylodis rhizoma and Atratcylodis macrocephalae rhizoma (IJTAA) on the change of weight and serum total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, free fatty acid, total lipid and phospholipid level in obese mice induced by high fat diet. Experimental groups were as follows ; Normal group was fed normal diet and administered distilled water during 7 weeks, Control group was fed high fat diet and administered distilled water during 7 weeks, Sample A group was fed high fat diet and administered IJTAA 500 ㎎/㎏/day/mouse during 7 weeks, Sample B group was fed high fat diet and administered IJTAA 700 ㎎/㎏/day/mouse during 7 weeks. The results were as follows ; 1. In Sample A group and Sample B group, There were significantly decreased in body weight, serum total cholesterol level, serum triglyceride level, serum free fatty acid level, serum total lipid level and serum phospholipid level in comparison with Control group. 2. In Sample A group and Sample B group, There were significantly increased in serum HDL-cholesterol level in comparison with Control group. 3. In Sample A group and Sample B group, There were decreased in serum LDL-cholesterol level in comparison with Control group. According to above results, I suggest IJTAA is able to be used for managing obesity by controllong body weight, serum total cholesterol level, serum triglyceride level, serum free fatty acid level, serum total lipid level and serum phospholipid level.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.489-493
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• 1989

쥐 하이브리도마 Alps 25-3를 이용하여 무혈청 배지 KM3의 각 성분이 세포증식과 항체생성에 미치는 영향을 조사하여 무혈청 배지를 최소화한 KMS를 결정하였다. Alps 25-3에 영향을 미치는 것으로 조사된 transferrin, ethanolamine 그리고 BSA만을 혼합하여 결정한 KMS를 다른 쥐 하이브리도마인 A4W, KW 그리고 HCGK에 적용시켰다. KM5를 KM3와 비교하였을 때 세포증식과 항체생산이 거의 동일한 양상을 보였다. 그러나 혈청배지와 비교하여 보면 최고 세포농도는 50-90%, 최종 단일클론항체의 농도는 85-100%의 수준을 보였다. 본 연구의 결과로 쥐 하이브리도마 세포를 무혈청 배지에서 배양시킬 때 필수적으로 첨가기어야 하는 성분들이 조사되었으므로 대량배양에 응용하거나 대사를 연구하는데 유용하게 이용되어지리라 본다.

• 대한약리학회지
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• 제2권1호
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• pp.99-109
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• 1966

It has been known that the pronounced hypotension resulting from hemorrhage gives rise to compensatory stimulation of the adrenosympathetic system, which leads to an increase of liberation of catecholamines from sympathetic nervous system and adrenal medulla. It is obvious, therefore, that numerous physiological and biochemical changes during the hemorrhagic hypotention might be mediated through the increased liberation of catecholamines. Although an extensive studies have been reported on changes of protein and carbohydrate metabolism in hemorrhagic shock a few studies on the changes of lipid metabolism have been reported. Levenson(1961) observed a marked increase of serum lipids content during hemorrhagic shock and also noticed a marked elevation of serum free fatty acids. He suggested that these effects were due to mobilization and accelerated metabolic breakdown of lipids which might be resulted by sympathetic stimulation as a cause. To elucidate the mechanism of this, author studied the change of serum free fatty acids and blood sugar with relation to catecholamines during experimentally induced hemorrhagic shock in dog. Healthy male mongrel dogs weighing approximately 15kg were used. Under the general anesthesia with pentobarbital, rapid hemorrhage was produced from the femoral artery maintaining blood pressure level of 40 mmHg measured by the manometer connected with the opposite femoral artery throughout the experiment. Serum free fatty acids(FFA) and blood sugar were measured by the methods of Dole(1956) and Folin-wu,(1920) respectively. Tissue catecholamine was measured by Shore and Olin method(1958) using Aminco-Bowman spectrophotofluorometer.

• 한국미생물·생명공학회지
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• 제18권1호
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• pp.61-65
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• 1990

Murine hybridoma 세포배양시 lectin 계통의 생산 증진제가 무혈청 배지의 단계적 유입에 의해 약 40mg/l의 단일항체를 생산함으로서 단일항체의 생산성을 증가시킬 수 있음이 확인됐으며, 또한 이 배양공법으로 무혈청 배지에서 연속배양이 불가능한 세포의 배양이 가능했다. 이같은 무혈청 배지의 단계적 유입공법으로 약 28 x $10^{-10}$mg/cells/h의 속도로 단일항체가 생산된 것에 비해, 10 혈청이 포함된 배지로는 연속 배양시 11.1 x $10^{-10}$mg/cells/h 의 속도로 생산됐으며 회분배양의 경우는 4.0 x $10^{-11}$mg/cells/h의 속도로 생산됐다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권5호
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• pp.583-590
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• 1996

The purpose of this study was to evaluate some factors in the bovine embryonic development from one-cell to blastocyst using modified synthetic oviduct fluid medium (mSOFM), after maturation and in vitro fertilization of the oocytes. The embryonic development to the blastocyst stage was assessed at 7-10 days after in vitro fertilization, and the total cells in the blastocysts were counted by staining nuclei with fluorochrome. Some commercial calf sera (CS) and a superovulated cow serum had different effects on the embryonic development to the blastocyst stage (8.6-21.4%), dependent upon their product lots, although the development might not be affected at least by serum progesterone levels. ${\beta}$-Mercaptoethanol (${\beta}$-ME) supplemented into mSOFM was effective to the embryonic development (27.8%), as well as the co-culture system with cumulus cells (19.5%). In a serum- and feeder cell-free culture using mSOFM containing several growth factors and ${\beta}$-ME instead of CS plus co-cultured cumulus cells, bovine serum albumin (BSA, fraction V), but not polyvinyl alcohol (PVA), was highly effective in embryonic development to the blastocyst stage, almost comparable to CS in the serum-contained culture (CS, BSA and PVA; 27.8, 19.5 and 5.7%, respectively). However, fatty acid free BSA rather reduced the number of developed blastocysts, compared with fraction V BSA (7.3 vs 29.4%). In the serum- and feeder cell-free culture, supplement of glucose to the medium (final 2.0 mM) stimulated the cell proliferation of developing embryos 120 hr after in vitro fertilization. These results indicated that a serum-free medium supplemented with ${\beta}$-ME could successfully support the development of bovine one-cell embryos to the blastocyst stage. Moreover, supplement of glucose and fatty acids to the medium might support preferably the development and cell proliferation of embryos.

• KSBB Journal
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• 제11권3호
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• pp.288-294
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• 1996

CHO 세포를 0.5%, 1 %. 2%, 3%, 4% 및 5% 놓도의 FBSd를 함유한 MEMa 배지에 $1{\times}10^4cells/\textrm{cm}^2 와 2{\times}10^4cells/\textrm{cm}^2$의 초기 농도로 정치배양하여 세포 성장과 EPO 생산 특성을 조사하였다. 혈청 농도와 접종 농도를 증가시킴에 따라 세포 증식 속도와 최대 세포 농도가 증가하는 경향을 보였으며, 적절한 혈청 농도의 선택이 세포 성장의 최적화에 필 요하다고 할 수 있다. 세포 성장 도중에 무혈청배지로 교환한 결과 세포 성장과 EPO 생산이 증가되었다. 배지를 교환하지 않았을 경우 세포 성장이 최대 ($2.1{\times}10^5cells/\textrm{cm}^2$)에 도달된 후 급격히 세포농도가 감소하는 높은 사멸율을 보이고, EPO의 최대 농도 도 2,380units/mL에 불과하였으나, 무혈청배지로 교환한 배양에서는 세포 성장이 크게 증가하여 $6.2{\times}105cells/$\textrm{cm}^2$의 최대 세포농도와 7,470units/mL 의 EPO 농도를 얻었다. 따라서 CHO 세포 성장과 E EPO 생산과는 상호 연관이 있으며, 세포배양으로 EPO 생산을 극대화하기 위해서는 CHO 세포의 고 농도 배양이 필요하다고 할 수 있다.

• Journal of Nutrition and Health
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• 제17권1호
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• pp.68-77
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• 1984

The present study was designed to observe the effects of soyprotein and casein with or without cholesterol on serum and liver lipids in male rats. The 6 experimental groups were as fellows ; SF ; soyprotein, cholesterol-free diet. SC ; soyprotein, 0.5% cholesterol added diet. CF ; casein, cholesterol-free diet CC ; casein,0.5% cholesterol added diet. SCF ; protein mixture of soyprotein and casein(1 : 1), cholesterol-free diet SCC ; mixed protein, 0.5% cholesterol added diet. The hypocholesterolemic and hypotriglyceridemic effects of soyprotein were observed at 3 weeks, but these effects disappeared at 6 weeks. The hypocholesterolemic effect of soyprotein was more obvious when the 0.5% cholesterol was supplemented in the diets. The serum free cholesterol level was not affected by the dietary protein source or the dietary cholesterol, therefore, the difference in serum total cholesterol among groups seems due to the difference in cholesterol esters. There was a tendency of a higher percentage of HDL in soyprotein groups compared to casein groups at 1 week, however, this tendency disappeared with time. The liver cholesterol and triglyceride contents were not differ among cholesterol-free diet groups, however, with addition of cholesterol, those of soyprotein groups were significantly lower than casein groups. The higher serum arginine/lysine ratio of soyprotein groups may offer the part of explanation of its hypocholesterolemic effect.

• 한국안광학회지
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• 제4권1호
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• pp.1-6
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• 1999

사람의 각막상피 세포를 배양하여 각막상피가 저절로 탈락해 나가는 과정을 이해하기 위해 무 혈청 배지에서 세포를 1주일 동안 배양하여 시간의 경과에 따른 세포의 소실과정을 조사하였다. 사람 각막상피 세포 주를 무 혈청 배지에서 배양하여 1, 2, 3, 6일에 세포의 형태적 관찰과 세포고사(Apoptosis) 정도를 측정하기 위해 Giemsa 염색, Hoechst 33342 형광 염색, 그리고 TUNEL(TdT-dUTP terminal nick-end labelling) assay를 시행하였다. 무 혈청 배지에서 세포를 배양하연 시간이 지남에 따라 세포의 모양이 변하였으며 망상의 세포 군집을 형성하였다. 많은 세포가 부유하여 작은 소체를 형성하였으며 세포고사 율은 3일 경과 후 50%를 나타냈다. 또한 TUNEL assay에 의해 단편화된 DNA도 관찰할 수 있었다. 본 연구의 결과는 영양 부족상태의 각막상피 세포가 세포고사를 거쳐 소모되고 탈락되는 것을 암시하고 있다.

• 한국축산식품학회지
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• 제43권6호
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• pp.1017-1030
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• 2023

Fetal bovine serum (FBS), which contains various nutrients, comprises 20% of the growth medium for cell-cultivated meat. However, ethical, cost, and scientific issues, necesitates identification of alternatives. In this study, we investigated commercially manufactured serum-free media capable of culturing Hanwoo satellite cells (HWSCs) to identify constituent proliferation enhancing factors. Six different serum-free media were selected, and the HWSC proliferation rates in these serum-free media were compared with that of control medium supplemented with 20% FBS. Among the six media, cell proliferation rates were higher only in StemFlex^TM Medium (SF) and Mesenchymal Stem Cell Growth Medium DXF (MS) than in the control medium. SF and MS contain high fibroblast growth factor 2 (FGF2) concentrations, and we found upregulated FGF2 protein expression in cells cultured in SF or MS. Activation of the fibroblast growth factor receptor 1 (FGFR1)-mediated signaling pathway and stimulation of muscle satellite cell proliferation-related factors were confirmed by the presence of related biomarkers (FGFR1, FRS2, Raf1, ERK, p38, Pax7, and MyoD) as indicated by quantitative polymerase chain reaction, western blotting, and immunocytochemistry. Moreover, PD173074, an FGFR1 inhibitor suppressed cell proliferation in SF and MS and downregulated related biomarkers (FGFR1, FRS2, Raf1, and ERK). The promotion of cell proliferation in SF and MS was therefore attributed to FGF2, which indicates that FGFR1 activation in muscle satellite cells may be a target for improving the efficiency of cell-cultivated meat production.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1299-1303
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• 2005

Human articular chondrocytes (HAC) were cultivated as a monolayer in a serum-free medium for primary culture (SFM-P). An optimized SFM-P provides $95\%$ proliferation rate of that obtainable from primary and secondary chondrocyte cultures grown in a control medium with serum. The gradual decrease in the amounts of synthesized glycosaminoglycan and type II collagen was improved by coating the culture dishes with type IV collagen and fibronectin. A significant improvement in the expression of type II collagen and aggrecan mRNA could be achieved. In addition, the monolayer cultures showed better synthesis of the extracellular matrices than alginate-bead cultures in SFM-P.