• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.619-624
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• 1999

The aim of this study was to evaluate the effects of oligosaccharides(fructo or isomalto ) contained in sponge cake on blood lipids and intestinal physiology in rats. The experimental diet was mixed with 30% sponge cakes. Normal male Sprague Dawley rats weighing about 530g were randomly assigned to three groups and placed experimental diets and deionized water at free access for 25 days. Rat in control group received a diet mixed with sponge cake containing sucrose only at 21.58g/100g diet and rats in the other two groups received diets mixed with sponge cakes of which 40% sucrose was replaced with each oligosaccharide. The results obtained were as follows: No significant differences in efficiency of food, liver weight and intestinal length were observed among groups. The cecal contents and cecal wall weights were increased more in fructooligosaccharide(FO) group than control. The pH of cecal contents decreased significantly in two oligosaccharide groups. The water contents of fresh feces in FO group were the highest and the those of two oligosaccharide groups were higher than that of control. Dry fecal weight increased significantly in isomaltooligosaccharide(IMO) group. Total serum cholesterol concentration was significantly lower in FO group. Serum triglyceride(TG) was not significantly different among three groups at p<0.05, but serum TG of FO group was lower than those of other groups. In conclusion, 40% replacement of sucrose with oligosaccharides in sponge cake may have beneficial effects on lipid metabolism and intestinal function in rats.

• Journal of the Korean Home Economics Association
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• v.30 no.4
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• pp.89-105
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• 1992

The purpose of this study was to see the effect of oral administration of reserpine (2mg/d) and tryptophan (40.35mg/d) on the serum amino acid concentrations and organ composition, food consumption, body weight, blood hematocrit(Hct) and hemoglobin(Hb) levels in Sprague-Dawley rats fed 6% or 20% casein diet. Any adverse effects of reserpine and tryptophan were not observed in animals, except that liver fat contents were increased in low protein group. In other words the administration of typtophan decreased liver fat contents in 6% casein and reserpine-treated 20% casein groups, but increased in reserpine-treated 6% casein group. But the low protein diet had significant adverse effects in animals. The 6% casein diet, therefore, had a tendency to decrease food consumption and body weight. The simillar tendency was shown in serum essential amino acid concentrations, organ weight and protein contents of liver and muscle. From the results, it would be safe to conclude that the oral administration of large deses of reserpine and tryptophan did not induce such a signifcant malnutrition as the low protein diet did.

• Journal of Nutrition and Health
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• v.17 no.2
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• pp.145-153
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• 1984

This study was performed to investigate the effect of various levels of Zn(0, 30, 3000 ppms) and different kinds of lipid(corn oil, sesame oil or butter.) in the diet upon lipid metabolism and Zn & Cu concentration in various organs and tissues in weanling rats. The results obtained were summarized as following : 1) Food consumptions, body weight gains and F.E.R.s showed no significant differences among groups. 2) The contents of total lipids and total cholesterols in serum tended to increase with increase in lipid saturation and Zn levels. Those in per g liver were higher in butter groups and tended to be higher in low Zn groups. Total lipids contents in per g muscle tended to be higher in butter and high Zn groups, but total cholesterols contents in per g muscle tended to be higher in corn oil and low Zn groups. 3) Liver, serum and fecal Zn concentrations tended to be higher in high Zn groups than other Zn groups. The Cu concentrations in liver and serum tended to be lower in high Zn groups. On the contrary, the Cu concentrations in urine tended to be higher in high Zn groups.

• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.141-151
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• 1992

Physiological changes of the murine hybridoma cell line $S3H5/\gamma{2bA2}$ during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.

• Journal of the East Asian Society of Dietary Life
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• v.8 no.3
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• pp.271-279
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• 1998

This study was conducted to compare the serum composition and urinary excretion in four institutional groups and the effects of intakes of good foods and harmful foods according to the Sasang constitiution for a short time on serum and ruine compositon. The contitutions of 33 female stucdents were classified by O-ring test and muscular strength test according to the Sasang constitution, The number of each institutional group was So-yang-in 11, So-em-in 10 and Tae-em-in 12. No subject belonged to Tae-yang-in There were no significant differences among contitutional groups in the values of most serum composition and urinary excretion Serum levels of total cholesterol, glucose, calcium and phosphorus in Tae-em-in was the highest among groups and the level of albumin and total protein in So-em-in was highest. The values of serum compositions changed after the 33 subjects took good foods and then harmful foods according to The Sasang constitution for a short time(5 days). Dietary behavior of the subjects had some effects among the constitutional groups in the values of most serum composition and urinary excretion. But the effects of dietary behavior on the serum composition and urinary excretion showed various trends in the four institutional groups.

• YAKHAK HOEJI
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• v.44 no.1
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• pp.71-79
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• 2000

Curcumin, the yellow pigment in turmeric, curry and mustard, has anti-inflammatory and anti-carcinogenic activities. In this study; we investigated the hypocholesterolemic and anti-atherogenic effect of curcumin in rabbits. Hypercholesterolemia was induced by feeding high cholesterol diet to male rabbits for 30 days, and the animals were then fed high cholesterol diet containing 0.1% (w/w) or 0.5% (w/w) curcumin for additional 30 days. Supplementation of 0.l% curcumin tended to lower serum total cholesterol and low density lipoprotein (LDL)-cholesterol levels and inhibit serum lipid peroxidation. In the 0.5% curcumin-supplemented group, serum total cholesterol was significantly lowered by 11.7%, LDL-cholesterol by 12.8% and lipid peroxidation by 47.9% compared to the control group. Hepatic cholesterol and triglyceride contents were also significantly lowered by 50.6% and 37.4%, respectively compared to the control group. Lipid staining of the arteries isolated from the curcumin-treated rabbits showed that curcumin significantly decreased formation of fatty streaks and atheromatous plaques on the intima of the arteries. These results demonstrated that curcumin lowered serum cholesterol concentration, hepatic cholesterol and triglyceride contents, and accumulation of cholesterol in the artery These cholesterol lowering effects of curcumin, together with its anti-oxidative and anti-inflammatory activities, may play some important roles in preventing atherosclerosis.

• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.429-438
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• 2005

This experiment was conducted to determine the effects of feeding CLA and coumarin on the changes of biochemical composition of velvet antler and blood serum in spotted deer (Cervus nippon). There were no significant differences between treatments in crude protein, crude fiber, and crude ash contents of velvet antler. The content of fatty acid did not differ between treatments, although there were significant differences (P<0.05) in C16:0 and C20:0. The content of collagen was significantly higher (P<0.05) in control than those in CLA and coumarin treatments. There were no significant differences between treatments in the contents of amino acids. Some constituents of blood showed much differences in comparison of pre and post-experiment for all treatments. Feeding of CLA and coumarin was not influential on the chemical composition of velvet antler and blood serum.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.5
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• pp.714-718
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• 2003

This study was conducted to determine the effect of DL-methionine (Met) and DL-methionine hydroxy analogue (MHA) supplementation on the growth performance, contents of serum amino acids and activities of digestive protease in broiler chicks from 0 to 3 weeks old . There were three treatments in this study: (1) control (basal diet), (2) 0.24% Met supplementation and (3) 0.368% MHA supplementation. The results showed that Met and MHA supplementation did not significantly (p>0.05) improve feed efficiency and body weight gain for broilers from 0-1, 0-2 and 0-3 weeks of age. The serum levels of homocystine, methionine and taurine were significantly (p<0.05) higher with supplementation of Met or MHA than with control. The pepsin activity of proventriculus was increased with (p<0.05) Met supplementation at 21 days of age and with MHA supplementation at 7 and 14 days of age. The trypsin activity was also increased (p<0.05) with MHA supplementation at 7 days of age. The chymotrypsin activity in pancreas and the dipeptidase activity in small intestinal mucosa and content were not affected (p>0.05) by Met or MHA supplementation.

• Journal of Nutrition and Health
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• v.36 no.7
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• pp.667-674
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• 2003

The study was performed to investigate the effects of isoflavone and/or grape seed oil supplementation on serum and liver lipid profiles and bone strength in ovariectomized female rats. Female Spraque-Dawley rats were assigned into 4 groups of ovariectomized (O), isoflavone (0.085 mg/100 g b.w/day) in ovariectomized rats, grape seed oil in ovariectomized rats and isoflavone and/or grape seed oil in ovariectomized rats. After 8 weeks, biochemical profiles of serum, liver and bone were analyzed. Total food intakes, body weight gains and FER (food efficiency ratio) were not statistically significantly different among groups. Total cholesterol, triglyceride and LDL-cholesterol levels in serum were decreased by fed of isoflavone and/or grape seed oils. However, crude lipid and total cholesterol contents in liver were not affect of isoflavone and/or grape seed oil. The hepatic glutathione contents were increased by isoflavone and/or grape seed oil fed. The hepatic glutathione-S-transferase activity in isoflavone and/or grape seed oil supplemented groups were higher than that O group. Bone (scapular and femur bone) dry weight, femur of max weight and bending strength were no significant difference among groups. Our finding suggest that isoflavone/grape seed oils might have potential role for serum lipid profiles improvement and bone strength in vivo.

• Korean Journal of Pharmacognosy
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• v.27 no.3
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• pp.246-253
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• 1996

Coptis rhizoma was extracted with methanol or hot water. Methanol extracts had higher berberine content than water extracts, whereas the contents of Ca, Mg, Zn and K were higher in the water extracts. Rats were fed on the purified diets rich in 0.12% cholesterol only or supplemented with 0.5% of each extracts for four weeks. The concentration of total serum cholesterol was significantly lower in the rats given the extracts, irrespective of the extractants. Although the concentration of serum HDL-cholesterol was comparable among the groups, the ratio of HDL-/total-cholesterol was higher in rats given the extracts. The concentration of serum free fatty acids and ketone body were significantly elevated in rats given the extracts. On the other hand, the extracts supplements significantly decreased the contents of liver cholesterol and triacylglycerol. The amount of fecal bile acids was markedly higher in rats given the extracts, especially in those given water extracts. The results show an effective hypocholesterolemic action of Coptis rhizoma and it seems that the lower concentration of serum and liver cholesterol is resulted from the increased fecal bile acids in rats.