• Biomedical Science Letters
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• v.2 no.1
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• pp.127-132
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• 1996

The karyotype and the concentration of serum protein were investigated in Korean unclassified mental retardees. The results were as follows. Fragile X chromosomes were identified in three patients, and the frequencies of fragile X chromosome were 4~15%. The concentration of serum protein was 5.73$\pm$0.89(g/dl), and the A/G ratio was 0.86$\pm$0.14 in fragile X syndrome patients. The concentration of serum protein was 6.83 $\pm$0.72(g/dl), and the A/G ratio was 0.87$\pm$0.17 unclassified mental retardees. The results revealed that the level of globulin concentration and A/G ratio in fragile X syndrome patients and unclassified mental retardees were lower than in normal group and Down's patients.

• Preventive Nutrition and Food Science
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• v.7 no.4
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• pp.367-372
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• 2002

This study investigated the effects of dietary soy protein and Ca on the lipid profiles of rats fed fat-enriched diets. Rats were divided into two groups and fed either a casein-low Ca (Exp I) or ISP-high Ca diet (Exp II) for a control period of 4 weeks. The two groups of rats were again subdivided into 4 groups and fed one of 4 experimental diets for another 4 weeks. The experimental diets consisted of 18% beef tallow and 1 % cholesterol, in which either 20 % casein or ISP with one of two levels of Ca, high (1 %) or low (0.1 %). The concentrations of total lipid, cholesterol and triglyceride in serum, liver and feces were determined. At the end of the control period, the serum total lipid and cholesterol concentrations were low in the rats fed ISP-high Ca diet (67~76% and 83~86%). During the next 4 week period, these concentrations remained significantly lower in rats fed the diets containing ISP and high Ca compared with those on casein and low Ca diets (p ＜ 0.05). Total lipid and cholesterol concentrations in feces were significantly higher in the ISP-high Ca dietary group at 4 weeks, and high in both high Ca groups at 8 weeks. This study demonstrates that both soy protein and Ca reduce serum and liver cholesterol, triglyceride, and total lipid in rats fed fat-enriched diet, and that they have an additive effect when combined.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1911-1915
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• 2012

Aim: New technologies for the early detection of pancreatic cancer (PC) are urgently needed. The aim of the present study was to screen for the potential protein biomarkers in serum using proteomic fingerprint technology. Methods: Magnetic beads combined with surface-enhanced laser desorption/ionization (SELDI) TOF MS were used to profile and compare the protein spectra of serum samples from 85 patients with pancreatic cancer, 50 patients with acute-on-chronic pancreatitis and 98 healthy blood donors. Proteomic patterns associated with pancreatic cancer were identified with Biomarker Patterns Software. Results: A total of 37 differential m/z peaks were identified that were related to PC (P < 0.01). A tree model of biomarkers was constructed with the software based on the three biomarkers (7762 Da, 8560 Da, 11654 Da), this showing excellent separation between pancreatic cancer and non-cancer., with a sensitivity of 93.3% and a specificity of 95.6%. Blind test data showed a sensitivity of 88% and a specificity of 91.4%. Conclusions: The results suggested that serum biomarkers for pancreatic cancer can be detected using SELDI-TOF-MS combined with magnetic beads. Application of combined biomarkers may provide a powerful and reliable diagnostic method for pancreatic cancer with a high sensitivity and specificity.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.561-568
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• 2012

The aim of this study was to introduce a simple method for isolation of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin from cow's milk, and peptides produced by enzymatic hydrolysis of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Whey protein were precipitated from whey by ammonium sulfate and, ${\alpha}$-lactalbumin and ${\beta}$-lactoglobulin were isolated using Hi Prep 26/60 Sephacryl S-100 column gel filtration chromatography. Bovine serum albumin and ${\beta}$-lactoglobulin were isolated by Mono-Q 5/50 GL column anion exchange chromatography of the 50% Ammonium Sulfate-supernatant. Isolated whey proteins were hydrolyzed by proteolytic alcalase. Tricine SDS-PAGE and reverse-phase HPLC analyses revealed that almost hydrolyzed all the ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Molecular weight of various peptides derived from alcalase hydrolysate were small molecular weight than 3.5 kDa.

• Journal of Nutrition and Health
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• v.30 no.6
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• pp.614-621
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• 1997

The experiments were performed to investigate the effects of protein and protein hydrolysates on lipid metabolism in the hyperlipidemic/hypercholesterolemic rat model induced by feeding fat-enriched diet. In Except 1 male rats were fed four semi-purified high fat and cholesterol diets that contained different nitrogen source, casein(C), casein hydrolysate(CH), corn gluten(G) and corn gluten hydrolysate(GH), for 6 weeks. In Expt. 2 rats were fed high fat and cholesterol diet for 4 weeks to induce hyperlipidemia and hypercholesterolemia. Then the rats were divided into 4 groups and were fed the four kinds of above experimental diets for 4 weeks consecutively. The contents of total lipid , cholesterol and triglyceride in blood, liver and feces were determined. Serum lipid concentrations of CH, G and GH were significantly lower than that of C. Serum cholesterol concentrations of hydrolysate groups(CH and GH) were significantly lower than those of intact protein groups(C and G). Serum HDL -cholesterol concentration tended to increase by hydrolysate intake. The total lipid, cholesterol contents in liver showed similarity results as above. Fecal lipid excretions of CH, G, and GH groups were significantly higher than that of C group. These results indicate that hypolipidemic and /or hypocholesterolemic effect of corn gluten or protein hydrolysates were detected in the process of inducing hyperlipidemia by high-fat and cholesterol diet or after inducing hyperlipidemia.

• Journal of Nutrition and Health
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• v.29 no.7
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• pp.788-805
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• 1996

This study was performed to investigate effects of examination-stress and protein supplementation on nitrogen metabolism and blood protein levels of Korean college students. Experiment was conducted at the beginning of a academic term and during midterm examination. During midterm examination, subjects were classified into two groups randomly : protein supplemental group(male n=6, female n=10) and placebo group(male n=4, female n=9). Protein capsules(2g/day) above 10% of indispensible amino acids requirement estimates were given to supplemental group for 10 days. At the begining of the term, male students(n=12) ingested 223.15mgN/kg/d, excreted 20.7mgN/kg/d in feces, and excreted 94.31mgN/kg/d in urine. Their apparent protein protein digestibility was 90.72%, true N balance was +100.11mgN/kg/d, and the mean maintenance N requirement of mixed Korena diet calculated was 112.13mgN/kg/d. Female students(n=19) ingested 171.44mgN/kg/d, excreted 22.13mgN/kg/d in feces, and excreted 122.92mgN/kg/d in urine. Their apparent protein digestibility was 86.76%, true N blance was + 18.39mgN/kg/d, and the mean maintenance N requirement calculated was 135.31mgN/kg/d. Blood levels of serum total protein, albumin, and BUN were within normal range. During midterm examination, fecal and urinary N excretions of female subjects(n=19) were increased, especially urea N markedly, and urea N/creatinine N ratio was augumented significantly. Apparent protein digestibility of male subjects(n=10) was decreased. Examination-stress showed 8.05mgN/kg/d (7.2%) increase of mean maintenance N requirement in male and 8.55mgN/kg/d(6.3%) increase in female students in comparison with that of the beginning of the term. Serum total protein and albumin levels showed no significant change, but serum transferrin level of female were decreased significantly. During midterm examination, females supplemented with protein capsules(2g/d)had no significant increase in fecal and urinary N excretions.

• The Korean Journal of Physiology and Pharmacology
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• v.3 no.1
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• pp.11-18
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• 1999

Nuclear factor $_{\kappa}B\;(NF-_{\kappa}B)$ activation is modulated by various protein kinases. Activation of $NF-_{\kappa}B$ is known to be important in the regulation of cell viability. The present study investigated the effect of inhibitors of protein tyrosine kinase (PTK), protein kinase C (PKC) and protein kinase A (PKA) on $NF-_{\kappa}B$ activity and the viability of bovine cerebral endothelial cells (BCECs). In serum-deprivation-induced BCEC death, low doses of $TNF{\alpha}$ showed a protective effect. $TNF{\alpha}$ induced $NF-_{\kappa}B$ activation within 4 h in serum-deprivation. PTK inhibitors (herbimycin A and genistein) and PKC inhibitor (calphostin C) prevented $NF-_{\kappa}B$ activation stimulated by $TNF{\alpha}.$ Likewise, these inhibitors prevented the protective effect of $TNF{\alpha}.$ In contrast to $TNF{\alpha}-stimulated\;NF-_{\kappa}B$ activity, basal $NF-_{\kappa}B$ activity of BCECs in media containing serum was suppressed only by calphostin C, but not by herbimycin A. As well BCEC death was also induced only by calphostin C in serum-condition. H 89, a PKA inhibitor, did not affect the basal and $TNF{\alpha}-stimulated\;NF-_{\kappa}B$ activities and the protective effect of $TNF{\alpha}$ on cell death. These data suggest that modulation of $NF-_{\kappa}B$ activation could be a possible mechanism for regulating cell viability by protein kinases in BCECs.

• Applied Biological Chemistry
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• v.37 no.5
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• pp.379-384
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• 1994

Casein or soybean protein was subjected to the reaction with caffeic acid-tyrosinase system at $30{\sim}35^{\circ}C$ pH 6.8 with aeration for 5 hr. The effects of the modified proteins on male Wistar rats were studied by pair-feeding of a cholesterol-free diet for 2 weeks. Significant decrease in protein digestibility for the rats fed with the modified proteins was observed. The feeding of modified protein from casein caused an enlargement of caecum. The concentration of serum cholesterol and triglyceride in the rats fed with modified proteins were mostly unchanged against the rats fed with untreated proteins. These results suggest that the decrease in protein digestibility induced by enzymic browning-reaction did not cause the decrease in concentration of serum cholesterol.

• Korean Journal of Veterinary Research
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• v.18 no.2
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• pp.69-71
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• 1978

Thirty heads of healthy pregnant and six heads of nonpregnant Holstein Friesian cows were selected for the purpose of serum chemical analysis. The partitions studied include serum total protein and total lipid with gestation periods (2.4.6.8. and 10 Months). The results were summarized as follows: 1. The mean value of serum total protein in 2 months of pregnant group was $7.70{\pm}0.28$ (7.4~8.3) g/100ml and then showed a tendency of decrease with the progress of pregnancy, that of 10 months group was figured $7.32{\pm}0.28$ (6.9~7.8) g/100ml. 2. The mean value of serum total lipids in 2 months of pregnant group was $335.67{\pm}14.69$ (300~386) mg/100ml and then showed a tendency of increase with the progress of pregnancy, that of 10 months group was figured $399.00{\pm}11.48$ (386~443) mg/100ml.

• Korean Journal of Veterinary Service
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• v.33 no.3
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• pp.293-297
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• 2010

Serum proteins of Korean native dogs (Gyeongju DongGyeong dogs) was analyzed by capillary electrophoresis system. Electrophoretic patterns showed six fractions. Total serum protein and ratio of albumin to globulin were $5.99{\pm}0.83$ (g/dl) and $1.41{\pm}0.29$ (g/dl) in DongGyeong dogs. Relative percentages of total serum albumin and $\alpha-1$, $\alpha-2$, $\beta-1$, $\beta-2$, $\gamma$-globulin fraction were $57.94{\pm}5.43$, $3.15{\pm}2.30$, $7.49{\pm}4.09$, $9.43{\pm}3.50$, $7.63{\pm}5.70$, and $14.36{\pm}7.63$, respectively. It was observed that $\beta$-globulin was higher than other fractions. The most striking alternation with age was founded in the $\gamma$-fractions. Also, it was observed that ratios of albumin to globulin in DongGyeong dogs were higher than on other dogs.