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http://dx.doi.org/10.7744/cnujas.2012.39.4.561

Isolation of whey protein and hydrolysis pattern of whey protein by proteolytic enzyme  

Renchinkhand, Renchinkhand (Department of Animal Biosystem Sciences, Chungnam National University)
Bae, Hyoung Churl (Department of Animal Biosystem Sciences, Chungnam National University)
Jeong, Seok Geun (National Institute of Animal Science, Rural Development Administration)
Nam, Myoung Soo (Department of Animal Biosystem Sciences, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.39, no.4, 2012 , pp. 561-568 More about this Journal
Abstract
The aim of this study was to introduce a simple method for isolation of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin from cow's milk, and peptides produced by enzymatic hydrolysis of ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Whey protein were precipitated from whey by ammonium sulfate and, ${\alpha}$-lactalbumin and ${\beta}$-lactoglobulin were isolated using Hi Prep 26/60 Sephacryl S-100 column gel filtration chromatography. Bovine serum albumin and ${\beta}$-lactoglobulin were isolated by Mono-Q 5/50 GL column anion exchange chromatography of the 50% Ammonium Sulfate-supernatant. Isolated whey proteins were hydrolyzed by proteolytic alcalase. Tricine SDS-PAGE and reverse-phase HPLC analyses revealed that almost hydrolyzed all the ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin and bovine serum albumin with alcalase. Molecular weight of various peptides derived from alcalase hydrolysate were small molecular weight than 3.5 kDa.
Keywords
Whey protein; Isolation; Enzymatic hydrolysis; Peptide;
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