• Title/Summary/Keyword: serological correlation

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Comparative analysis of serological tests and fecal detection in the diagnosis of Mycobacterium avium subspecies paratuberculosis infection

  • Park, Hong-Tae;Ha, Seungmin;Park, Hyun-Eui;Shim, Soojin;Hur, Tai Young;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.60 no.3
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    • pp.117-122
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    • 2020
  • Johne's disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a chronic, wasting infectious disease in ruminants that causes enormous economic losses to the dairy and beef cattle industries. The most effective way to eradicate JD is to detect infected individuals as early as possible and remove them from the herd. However, it is difficult to detect infected individuals early with the currently using diagnostic methods. Two serological diagnostic kits commercially used worldwide and a fecal detection test were compared using 298 serum samples and feces of cattle in this study to present an efficient diagnostic method. Although there was a high correlation between the 2 serological diagnostic kits (R2 = 0.7473), kit A showed a higher serological positive rate. However, the correlation between fecal tests and serological diagnosis was very low. MAP was also detected in fecal tests in many serologically negative individuals. In the periodical diagnosis of JD, MAP was detected in the feces of only cows with the higher antibody titer to MAP. These results suggest that for effective eradication of JD, early detection of infected individuals by fecal tests together with the serological tests currently in use and by removal of infected individuals are needed.

Immunohistochemical and serological observations on Neospora caninum infection in Korean indigenous cattle and calves of abnormal deliveries (한우 및 이상산 송아지의 Neospora caninum의 감염에 대한 면역조직화학적 및 혈청학적 관찰)

  • 손성봉;정원일;정규식;이차수
    • Korean Journal of Veterinary Service
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    • v.27 no.1
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    • pp.53-62
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    • 2004
  • The present study was carried out to investigate the prevalence of Neospora caninum infection in Korean indigenous cattle and calves of abnormal deliveries and focus on correlation between malformation and N caninum infection. To determine the prevalence of antibodies to N caninum, sera of 473 Korean indigenous cattle from slaughter house were tested for N caninum antibodies using indirect fluorescence assay. Of the 473 cattle sera, 9.5% (45/473) showed positive against N caninum. Regional seropositive rates of the samples were 16.7% (5/85), 11.0% (11/100), 8.8% (21/240) and 5.9% (5/85) at Kyonggi, Gyeongbuk, Daegu and Kyongnam province, respectively. In female, seropositive rates were 17.5% (25/143) and 6.1% (20/330) in male. During the period from march 2000 to August 2001, 55 abnormal deliveries of Korean indigenous cattle including abortion, stillbirth and congenital malformation were examined by histopathological, immunohistochemical and serological methods for evidence of N caninum infection. Of the 55 abnormal deliveries, only 5 calves showed positive reaction against N caninum in serological test. In microscopical observation, gliosis and nonsuppurative myositis were observed. However, Neospora-like organisms were not detected by either periodic acid-schiff (PAS) reaction or immunohistochemical technique. Taken together all these data, this study indicate that N caninum infection was widespread in breeding farms of Korean native cattle, but correlation between malformation and N caninum infection was not recognized.

Comparison of serological methods for detection of avian influenza virus antibodies (가금인플루엔자 바이러스 항체검출을 위한 혈청학적 진단법 비교)

  • Han, Myung-guk;Park, Kyoung-yoon;Kwon, Yong-kuk;Kim, Jae-hong
    • Korean Journal of Veterinary Research
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    • v.42 no.1
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    • pp.73-80
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    • 2002
  • An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5ug/100ul, 1:200 and 0.03ug/100ul, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p < 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.

Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis (소 브루셀라병 표준시험관응집반응법 진단효율 평가)

  • Sung, So-Ra;Kim, Ji-Yeon;Her, Moon;Lee, Kichan;Gu, Jeong-Hui;Kang, Sung-Il;Lee, Hyang-Keun;Kim, Suk-Mi;Jung, Suk-Chan
    • Korean Journal of Veterinary Service
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    • v.35 no.4
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    • pp.269-273
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    • 2012
  • A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

Diversity of Bradyrhizobium japonicum with Different Colony Morphology in Intrinsic Antibiotic Resistance, Serological Property, and Protein Profile (콩 근류균(根瘤菌) Bradyrhizobium japonicum의 취락형태별(聚落形態別) 항생제(抗生劑) 반응(反應)과 혈청형(血淸型) 및 단백질전기영동(蛋白質電氣泳動) 유형(類型)의 다양성(多樣性))

  • Kang, Ui-Gum;Ha, Ho-Sung;Jung, Yeun-Tae;Kang, Hang-Won;Yun, Han-Dae;Ha, Yeong-Lae
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.60-66
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    • 1996
  • Bradyrhizobium japonicum with different colony morphology populated in five Yeongnam soils of Korea was examined for intrinsic antibiotic resistance to eight antibiotics, serological property by immunoblot and immunodiffusion, and protein profile differentiation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Colony morphological distribution of one hundred and twenty B. japonicum isolates was 47% for "dry". 41% for "wet", and 12% for "dry/wet" type. The total isolates showed such a strong correlation between the morphology and antibiotic resistance. Colony morphology, which though was dominantly consisted of the same type within a serogroup, wasn't absolutely linked to serological property of B. japonicum. Based on these data, colony morphology was too simple to identify variations with B. japonicum isolates : antibiotic resistance such complicated compared with serological analyses.

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Serotaxonomical Analyses of Some Streptomyces Strains Using Antibodies against Cell Envelope (균사 외피 항체를 이용한 Streptomyces 속 균주들의 혈청학적 유사성 분석)

  • Jo, Sung-Kee;Kim, Jae-Heon
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.137-141
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    • 2007
  • The similarities among five strains of Streptomyces were measured by the serological methods. Antigens were prepared by dissolving cell envelope fractions in Tween 20 buffer and antisera were produced from the immunized rabbits. Immunodiffusion studies and ELISA results showed that the degree of antigen-antibody reaction was not exactly matched to the taxonomic distance; i. e. the strains of the cluster group A exhibited low level of cross-reactions each other, while relatively strong cross-reactions were observed between Streptomyces lavendulae of cluster group F and Streptomyces viridochromogenes of cluster group A.

A Serological Study on Toxoplasma gondii Infection Among People in South of Tehran, Iran

  • Salahi-Moghaddam, Abdoreza;Hafizi, Abbas
    • Parasites, Hosts and Diseases
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    • v.47 no.1
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    • pp.61-63
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    • 2009
  • Although Toxoplasma gondii infection generally shows subclinical courses in adults, it may have severe pathologic effects on the fetus. For a survey of toxoplasmosis in Shahr-e-Rey area, south of Tehran, Iran, we conducted a serological study on 1,187 general populations using indirect fluorescent antibody (IFA) test. In this cross sectional study, cases were selected randomly by a local primary health care (PHC) system. Blood samples were collected in microcapillary tubes and sera were examined by IFA after centrifugation. It has been shown that 68.3% of cases in all age groups had IFA titer of 1:10 and higher, which we consider as positive cases, and the rate for 1:400 or over was 30.2%. There was meaningful correlation between anti-Toxoplasma antibody titers and age, sex, and occupation of subjected people (P < 0.05%). The incidence was higher in females and highest in the children age group (10-14 years old). The present study demonstrates high prevalence of Toxoplasma infection among the people in south of Tehran, Iran.

Serological responses and protection levels in chickens administered with Newcastle disease vaccines

  • Geumji Seung;Jiye Kim;Hyobi Kim;Ji-Yeon Kim;Yang-Ho Jang;Yeon-Hee Kim;Moon Her;Seong-Joon Yi;Keun-Woo Lee;Il Jang;Young Ju Lee
    • Korean Journal of Veterinary Research
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    • v.62 no.4
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    • pp.29.1-29.7
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    • 2022
  • Vaccination against Newcastle disease (ND) is the most effective means of controlling the disease, and these vaccines are commercialized only after their safety and effectiveness have been verified through tests that comply with Korean Standards of National Lot Release for Veterinary Biologics. This study investigated whether a relatively convenient and safe serological test can be used in place of the challenge test using highly virulent ND virus. Hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA) were considered positive of log2 2 or more and cutoff value of 200 or more, respectively, in both live and inactivated vaccines. However, when the antibody levels of the live and inactivated vaccines induced using the Ulster 2C, KBNP-C4152R2L, and K148/08 strains were compared, the antibody titers for inactivated vaccines were significantly higher than those for live vaccines in both the HI assay and ELISA. A strong positive correlation was observed between HI and ELISA antibody titers. The live vaccines corresponded to a survival rates of ≥ 80% and the inactivated vaccines corresponded to 100% survival rates. This study confirmed that standard efficacy tests can serve as serological tests, and can replace the challenge test and that the vaccine approval process can be improved.

Effect of Hematological Factors on the Risk Index of Cardiovascular Disease (혈액학적 인자가 심혈관 질환 위험지수에 미치는 영향)

  • Hyun An;Hyun-Seo Yoon;Chung-Mu Park
    • Journal of radiological science and technology
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    • v.46 no.4
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    • pp.303-313
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    • 2023
  • This study aimed to investigate the relevance of cardiovascular disease risk factors AI and AIP, divided into three groups, among 300 individuals who underwent health checkups at the hospital. Various variables such as Age, Sex, BMI, WC, TC, TG, HDL-C, LDL-C, FBS, HbA1C, SBP, DBP, HR, AI (TC/HDL-C), and AIP (log(TG/HDL-C)) were analyzed using statistical methods including frequency analysis, cross-tabulation, one-way ANOVA, Pearson's correlation analysis, and multiple linear regression analysis. The cross-analysis based on cardiovascular disease risk criteria revealed that men and individuals in their 50s had higher cardiovascular disease risk based on AI and AIP. Significant differences were observed in TG, TC, HDL-C, LDL-C, SBP, DBP, AI (TC/HDL-C), and AIP (log(TG/HDL-C)) according to AI criteria. For the AIP criteria, TG, TC, HDL-C, FBS, HbA1C, HR, AI (TC/HDL-C), and AIP (log(TG/HDL-C)) were identified as cardiovascular disease risk factors. FBS and HbA1c showed the highest positive correlation In the correlation analysis, followed by TC and LDL-C. The lowest positive correlation was observed between LDL-C and DBP. In terms of negative correlation, HDL-C and AI had the highest negative correlation, while LDL-C and TG showed the lowest negative correlation. Multiple regression analysis indicated that the AI and AIP risk criteria had explanatory powers of 73.6% and 72.5%, respectively. HDL-C had the greatest negative effect on the AI risk criterion, while TG had the most significant influence on the AIP risk criterion. In conclusion, while other serological variables are important, managing HDL-C and TG levels may help reduce the risk of cardiovascular disease.

Serological cross-reaction with Brucella abortus antigen extracted by sodium dodecyl sulfate and Yersinia enterocolitica 0:9 (SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구)

  • Lim, Yoon-kyu;Yang, Ki-chun;Lee, Kyung-kap;Park, Jun-hong;Lee, Du-sik;Park, Yong-ho;Kang, Seung-won;Mok, Ji-won;Lee, Yong-soon
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.143-148
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    • 1995
  • Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.

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