• 대한수의학회지
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• 제47권3호
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• pp.273-279
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• 2007

Swine influenza is an acute, infectious respiratory disease caused by type A influenza viruses in pigs. In the previous studies, serological surveys have indicated the presence of H3N2 swine influenza virus (SIV) since 1995 in Korea. And the percentage of the antibody-positive rate was 39.12% in the survey determining the prevalence of H1N1 SIV antibodies in 2002. The purpose of this study was therefore to investigate the sero-prevalence of SIV regard to the age of the pig and the season between June 2004 and May 2005. In this study, a total of 932 sera were used. These sera were randomly selected from blood samples, which were submitted to Department of Veterinary Pathology, Kangwon National University and Department of Veterinary Virology, Seoul National University from June 2004 to May 2005. These sera have been tested by ELISA test kit (IDEXX Lab, USA) for the SIV H3N2, H1N1 respectively. SAS version 9.1 was used for the statistical analysis based on the age of the pig and the season. The overall sero-prevalence of the antibody against H3N2 SIV was 20.82% (194/932). The overall sero-prevalence of the antibody against H1N1 SIV was 37.23% (347/932). The overall dual sero-prevalence of the antibody against H3N2 and H1N1 SIV was 10.62% (99/932). H3N2 has significant difference in statistically regarding the age of the pig and the season (p<0.0001). H1N1 has significant difference in statistically regarding the age of the pig (p<0.0001) but has not significant difference in statistically regarding the season (p=0.5882).

• 대한수의학회지
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• 제57권4호
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• pp.209-214
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• 2017

Wild raccoon dogs (Nyctereutes procyonoides koreensis) may play a role transmitting several pathogens to humans and pet animals. Information concerning the incidence of rabies, canine distemper virus (CDV), canine parvovirus (CPV), canine adenovirus type 2 (CAdV-2), canine parainfluenza virus type 5 (CPIV-5), and canine herpesvirus (CHV) is needed in wild raccoon dogs. In total, 62 brain samples of raccoon dogs were examined for rabies virus (RABV) and CDV, and 49 lung samples were screened for CDV, CAdV-2, CPIV-5, and CHV. No RABV, CAdV-2, CPIV-5, or CHV was identified, but nine CDV antigens (8.1%, 9/111) were detected. Moreover, 174 serum samples from wild raccoon dogs were screened for antibodies against the five major viral pathogens. The overall sero-surveillance against CDV, CPV, CAdV-2, CPIV-5, and CHV in wild raccoon dogs was 60.3%, 52.9%, 59.8%, 23.6%, and 10.3%, respectively. Comparisons of the sero-surveillance of the five pathogens showed that raccoon dogs of Gyeonggi province have slightly higher sero-positive rates against CDV, CPV, and CHV than those of Gangwon province. These results indicate high incidences of CDV, CPV, and CAdV-2 in wild raccoon dogs of two Korean provinces and a latent risk of pathogen transmission to companion and domestic animals.

• 한국지능시스템학회논문지
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• 제25권5호
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• pp.457-463
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• 2015

본 논문은 적시학습을 위한 집단지성 기반 오답노트 시스템의 필요성과 개념을 제시하고 활용도 및 만족도를 보인다. 기존 오답노트 시스템은 평가문항에 대한 정답 여부를 체크하여 응답 결과가 오답인 문항에 대하여 일괄적인 정답 해설을 제공하는 특징을 갖고 있다. 이러한 특징은 학습자가 평가문항을 잘 이해하지 못하고 운 좋게 맞은 문항과 개별 학습자들의 오답 선택 과정에 대한 오류를 적절하게 피드백 받지 못하기 때문에 오답분석과 피드백 측면에서 많은 개선을 필요로 하고 있다. 본 논문에서는 이러한 '점수의 오류'를 개선하여 학습자의 취약점을 보완하는 새로운 방법인 SERO(Stability Emergency Risk Opportunity) 오답노트를 제안한다. SERO 오답노트는 자신이 풀어낸 평가문항들을 4가지 유형(S유형, E유형, R유형, O유형)으로 분류하고, 정답 해설 뿐만 아니라 집단지성을 이용한 다양한 오답노트 해설들을 제시하여 학습의 만족도를 높인다.

• Parasites, Hosts and Diseases
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• 제35권3호
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• pp.171-180
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• 1997

쭈쭈가무시병을 매개하는 털진드기에 대한 연구는 주로 경기도와 강원도 일대의 중부지방과 전 북. 전남 및 경남 등 남부지방에서 부분적으로 이루어져 왔다. 저자들은 지금까지 전혀 연구된 바 없는 중남부 내륙지t:1 12개 지역에서 들쥐와 털진드기를 채집 조사하여 다음과 같은 결과를 얻었다 채집된 총 177개체의 들쥐 중 등줄쥐(Aponemwosrulif)가 154(87.0%)였다. 들쥐에 기생 고 있던 털진드기 25.707개체를 채집하여 동정한 결과 14종이 확인되었는데 대잎털진드기 (Leptotrombidium pallidum)가 79.8%로 우점종이었고. 다음이 수염털진드기(1.pohole)로 8.9% 였다 쭈쭈가무시병의 매개종인 대잎털진드기가 12개 조사지역 중 11개 지역에서 우점종이었고 밀도가 가장 높은 지역인 상주시 초오 2동의 chigger index는 201.8이었고 가장 낮은 지역인 충주시 단월동은 40.7이었다 또 활순털진드기(L. scueuaye)의 경우 김천시 운수리에서만 chigger index 61.6으로 높은 개체군 밀토를 보였고 금릉군 여배리와 미평리에서는 각각 1.4와 1.2였으며 기타 지역에서는 극소수 채집되었거나 전혀 채집되지 않았다 활순털진드기 서식 북방 한계선은 전북 익산군 - 충남 공주군 - 경북 김천시 - 경북 경주시 - 경북 영덕군으로 연평균기온이 $10.0^{\circ}C$ 이상인 지역과 일치하였다. 등줄쥐 157개체의 혈청을 간접형광항체법으로 검사한 결과 60 개체가 양성으로 항체양성률 38.2%였고 가장 높은 지역은 금릉군 여배리로 85.7%였고 가장 낮은 지역은 문경시 고요리의 7. l%였으며 김천시 운수리에서는 양성률 0으로 나타났다 혈청형은 Karp 48.3% william 1.7% 및 Kuroki 3.3%였으며. 나머지 46.7%(28개체)는 2개 혈청형 이상이 같 은 항체가를 보여 혈청형을 확인할 수 없었다.

• Journal of Veterinary Science
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• 제21권4호
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• pp.63.1-63.9
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• 2020

Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

• 대한수의학회지
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• 제64권1호
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• pp.3.1-3.8
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• 2024

Canine distemper virus (CDV), canine adenovirus type 2 (CAV-2), canine parvovirus (CPV), and canine parainfluenza virus 5 (CPIV-5) are the major viral pathogens in dogs. Despite the availability of vaccines for dogs against these 4 viral pathogens, investigations of antibodies against these pathogens have rarely been reported in South Korea. In this study, we investigated the recent incidence of viral diseases in dogs and conducted sero-surveillance for CDV, CAV-2, CPV, and CPIV-5 in Korean dogs. The most frequently diagnosed canine viral disease in Korean dog samples from 2000 to 2022 was CPV infection, which accounted for 48.7% (464/953) of the cases. A total of 400 dog serum samples collected between 2019 and 2022 were screened for the presence of virus-neutralizing antibodies against CDV, CAV-2, CPV, and CPIV-5. The overall seropositivity rates for CDV, CAV-2, CPV, and CPIV-5 were 83.8%, 77.8%, 99.3%, and 82.0%, respectively. The protection rate against CPV was the highest (98.3%) and that against CAV-2 was the lowest (44.8%) in dog sera. Male and female dogs showed no significant differences in seropositivity rates. CDV and CPIV-5 seropositivity increased with age in dogs, and the highest incidence and seropositivity rates of CPV indicated that Korean dogs have been continuously exposed to wild CPV, and that CPV is a pathogen that urgently requires attention among canine viral diseases.

• 한국축산식품학회지
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• 제36권6호
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• pp.779-786
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• 2016

This study aimed to evaluate the prevalence of Listeria monocytogenes on livestock farms in Korea and determine their serotypes and genetic correlations. Twenty-five livestock farms in Korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. Samples were enriched in LEB (Listeria enrichment broth) and Fraser broth media, and then plated on Palcam agar. The isolates were identified by PCR and 16S rRNA gene sequencing. Then, the sero-types, presence of virulence genes (actA, inlA, inlB, plcB, and hlyA), and antibiotic resistance were determined. Genetic correlations among the isolates were evaluated by analyzing the restriction digest pattern with AscI. Of the 2,018 samples, only 3 (0.15%) soil samples (FI-1-FI-3) from 1 farm in the south east region were positive for L. monocytogenes. Based on biochemical tests and multiplex PCR, the serotype of the isolates were 4ab (FI-1 and FI-3) and 3a (FI-2), which are not common in foodborne L. monocytogenes. The 3a sero-type isolate was positive for all tested virulence genes, whereas the 4ab serotype isolates were only positive for hlyA, actA, and inlA. The isolates were resistant to all 12 tested antibiotics, especially FI-3. The genetic correlations among the isolates were 100% for those of the same serotype and 26.3% for those of different serotypes. These results indicate that the prevalence of L. monocytogenes on livestock farms in Korea is low; however, the isolates are pathogenic and antibiotic resistant.

• 한국가금학회지
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• 제40권4호
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• pp.379-388
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• 2013

Avian paramyxovirus (APMV) type 4 and 6 were isolated during an avian influenza (AI) surveillance program of wild birds. This study also conducted experimental infection of wild-bird-origin APMV type 4 and 6 in specific pathogen free (SPF) chickens to study pathogenicity and transmission within domestic flocks. In addition, serological prevalence data of APMV type 4 and 6 in domestic fowls was conducted with chicken sera collected from 2007 to 2009 in order to understand infection status. The results of the animal experiment showed that APMV type 4 and 6 had the ability to infect chickens with sero-conversion and to transmit the virus from infected birds to contacted birds, but showed low pathogenicity. Serological tests revealed that APMV type 4 was widespread in the poultry industry, especially in layer flocks, but the positive rate for APMV type 6 was very low. This study concluded that wild bird-origin APMV type 4 and 6 could infect the chickens by inter-species transmission and the seroprevalence of APMV type 4 was quite high in Korean poultry. However, since almost all the chicken flocks had a high level of antibody titer against APMV type 1, there was possibility of cross reaction between APMV type 1 and 4, which made the interpretations more complicated. In order to understand infection status in the natural environment, additional study is necessary regarding the seroprevalence of APMV type 4 and 6 in the wild bird population.

• 센서학회지
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• 제4권4호
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• pp.16-22
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• 1995

메탄, 프로판가스를 검출하기 위하여 ${\gamma}-Al_{2}O_{3}$ 담체에, Pd 촉매를 섞어서 접촉연소식 센서 및 digital 계측기를 제작한 후 그 소자특성 및 계측기로서 응용가능성을 조사하였다. 본 연구에서 제조된 센서는 인가전압 2 V에 소모전력은 700mW가 나왔고, 출력전압변화는 1,000ppm의 프로판의 경우 약 700mV, 메탄의 경우 500mV 변화를 나타냈다. 계측기에서는 2,400ppm을 10회 반복적으로 주입시켜 측정하였다. 그 결과로, ${\pm}25ppm({\pm}1%)$의 오차를 보였고, 감도, 선형성, 재현성이 우수한 것을 볼 수 있었다.

• 한국동물위생학회지
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• 제24권4호
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• pp.359-367
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• 2001

A serological survey was performed to establish basic data for the prevalence of antibodies to some major diseases of domesticated boar serum samples from January to December 2000. Sera collected in breeding farms in Gyeongbuk province were tested for Aujeszky's disease virus(ADV), Porcine reproductive and respiratory syndrome virus(PRRSV), Porcine parvovirus(PPV), Japanese encephalitis virus (JEV), Bordetella bronchiseptica(B bronchiseptica), Mycoplasma ; APP), Toxoplasma, and Brucella. There was no antibody to ADV in domesticated boars serum samples detected by Anti-ADV-gpI assay kit. Sero-positive samples to PRRS by IFA were 0.9%(3/330) The HI titers to PPV ranged variously from less than 10 to over 1,280. Two hundred ninety-four out of 330 tested sera showed HI titer of less than 10. In HI test to JEV, 90.3% of the sera (298/330) were below 10. The majority of the serum samples had low prevalence of the antibody B bronchiseptica. ELISA titers to M hyopneumoniae ranged variously from $\leq$ 10 to $\geq$ 1,280. Antibody titers to A pleuropneumoniae type 2(APP2) and type 5(APP5) were 58.2% and 52.7%, respectively, and the tested samples showing ELISA antibody titers of less than 20. There was no significant geographical difference between APP2 and APP5 in this study. In the antibody test of Toxoplasma, 11.5%(38/330) were positive and samples were all negative in sera test of Brucella.