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Application of the Ecotoxicological Standard Method using Population Growth Inhibition of Marine Phytoplankton (식물플랑크톤의 개체군성장저해율을 이용한 해양생태독성 시험방법에 관한 연구)

  • Lee, Sung-Min;Park, Gyung-Soo;An, Kyoung-Ho;Park, Soung-Yun;Lee, Sang-Hee
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.112-120
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    • 2008
  • A series of experiments were conducted to establish a marine ecotoxicological standard method using marine primary producers, Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, Tetraselmis suecica as candidate test species. Marine diatom, S. costatum was selected as standard test species in terms of the ecological roles and international uses as test species. Test methods and test acceptability criteria are as follows; $72{\sim}96$ hr population growth inhibition $EC_{50}$ as endpoint. Static non-renewal method, and population growth rates over 0.04/hr in control as test acceptability criterium. S. costatum is widely distributed in the world ocean and used for standard species of marine toxicity test method by ISO (International Standardization Organization). Possible salinity ranges for this test method are $20{\sim}35\;psu$, and reproducibility and interlaboratory test results were consistent through the calibration tests. Sensitivity of the test method was comparable or better than other toxicity tests such as rotifer neonate mortality, bioluminescent bacterial inhibition, seaweed sporulation and sea urchin fertilization tests.

An Analysis of High School Students' Activity on Problem-finding in III-structured Scientific Problem Situation (낮게 구조화된 과학적 문제 상황에서 고등학생들의 문제발견 활동 분석)

  • Ryu, Si-Kyung;Park, Jong-Seok
    • Journal of The Korean Association For Science Education
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    • v.26 no.6
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    • pp.765-774
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    • 2006
  • The purpose of this study was to suggest an instructional direction for improving scientific problem-finding ability. For this purpose, the present study made an in-depth analysis about activity on problem finding tasks of high school students in an ill-structured scientific problem situation. Subjects were divided into two groups (cooperative and individual) and two kinds of problem finding tasks were administered to two groups. Results indicated that a cooperative activity on problem finding happened to a series of steps exploring problem situation, expressing knowledge and experience, discussing provisional problems, creating various problems and selecting the best problem. Besides, a cooperative activity on problem finding depended heavily on prior knowledge and experience, and in the meantime, various scientific concepts turned out to naturally be expressed. As for the problems found out during a cooperative activity, their scores in creativity factors, including the degree of agreement in original problem selection came out to be on the whole, as excellent. In addition, the types of the problems found out in open problem situation showed that they were more various than those found out in closed problem situation. Subjects perceived that activity on problem finding had positive influence on scientific concept and science process skills. Findings of this study have the following educational implications: First, it is needed to prepare for educational environment that enables students to explore various knowledge and information. Second, the offering of various opportunities is needed to enlarge the scope of scientific knowledge and experience. Third, it is needed to prepare for a study atmosphere that lets students express their knowledge and experiences freely.

Development of Sediment Toxicity Test Protocols using Korean Indigenous Marine Benthic Amphipods (국내산 저서 단각류를 이용한 퇴적물 독성시험법 개발에 관한 연구)

  • Lee, Jung-Suk;Lee, Seung-Min;Park, Gyung-Soo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.147-155
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    • 2008
  • A series of experiments were conducted to find standard test organisms and to develop test protocols for sediment toxicity tests using indigenous amphipods inhabited in Korean coastal environments. The indigenous amphipods Mandibulophoxus mai and Monocorophium acherusicum were well associated with various sediment substrates from sand to mud. The tolerance limits to various physico-chemical factors affecting bioassay results such as temperature, salinity and total ammonium as well as the sensitivities to contaminants in water and sediments were investigated using M. mai and M. acherusicum in the present study. These amphipods were tolerable to the adequate ranges of salinity ($10{\sim}30\;psu$), temperature ($10{\sim}25^{\circ}C$) and ammonia (<50 ppm). They have relevant sensitivities to the reference toxicants, dissolved cadmium as well as other metals and organic pollutants, when compared to the standard test species used in other countries. Field-sediment toxicity tests revealed that M. mai would be more sensitive to sediment-associated pollutants than M. acherusicum, while the sensitivity of M. acherusicum was comparable to those of other sediment test species in other countries. Overall results of this first attempt to develop an amphipod sediment toxicity test protocol in Korea indicated that M. mai and M. acherusicum should be applicable in the toxicity assessment of contaminated sediments, following the further evaluation encompassing various ecological and toxicological evaluation and the standardization of test method.

Development of Marine Toxicity Standard Method for Marine Luminescent Bacteria: Introduction of N-Tox test (해양성 발광박테리아를 이용한 해양환경 독성평가 시험법 개발: N-Tox test)

  • Lee, Kyu-Tae;Park, Gyung-Soo;Kim, Pyoung-Joong
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.156-163
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    • 2008
  • Luminescent bacterial toxicity test was first introduced in the early 1980s, registered as international standard method in 1998 and now widely used as a common toxicity test method. This toxicity test uses luminescent bacterium, Vibrio fischeri, originated from marine environment as a test organism. The degree of toxicity can be evaluated from the comparison of luminescent emission intensity between control and treatment groups to toxicants and materials from various environmental matrix for 30 min. This test can be carried out by using commercial products and its results are sensitive and precise. This research is on the feasibility of adopting luminescent bacterial test as a domestic standard test protocol. Using commercial products, a series of experiments were conducted to identify the precision and accuracy of injection volume and light emission, and to evaluate concentration-response relationship between chemical concentrations and light emissions. Also, the feasibility of the application to environmental media and quality assurance/quality control were checked. The results of serial toxicity tests revealed that the preliminary luminescent bacterial toxicity test was robust and suitable as a standard method.

A Predictive Bearing Anomaly Detection Model Using the SWT-SVD Preprocessing Algorithm (SWT-SVD 전처리 알고리즘을 적용한 예측적 베어링 이상탐지 모델)

  • So-hyang Bak;Kwanghoon Pio Kim
    • Journal of Internet Computing and Services
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    • v.25 no.1
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    • pp.109-121
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    • 2024
  • In various manufacturing processes such as textiles and automobiles, when equipment breaks down or stops, the machines do not work, which leads to time and financial losses for the company. Therefore, it is important to detect equipment abnormalities in advance so that equipment failures can be predicted and repaired before they occur. Most equipment failures are caused by bearing failures, which are essential parts of equipment, and detection bearing anomaly is the essence of PHM(Prognostics and Health Management) research. In this paper, we propose a preprocessing algorithm called SWT-SVD, which analyzes vibration signals from bearings and apply it to an anomaly transformer, one of the time series anomaly detection model networks, to implement bearing anomaly detection model. Vibration signals from the bearing manufacturing process contain noise due to the real-time generation of sensor values. To reduce noise in vibration signals, we use the Stationary Wavelet Transform to extract frequency components and perform preprocessing to extract meaningful features through the Singular Value Decomposition algorithm. For experimental validation of the proposed SWT-SVD preprocessing method in the bearing anomaly detection model, we utilize the PHM-2012-Challenge dataset provided by the IEEE PHM Conference. The experimental results demonstrate significant performance with an accuracy of 0.98 and an F1-Score of 0.97. Additionally, to substantiate performance improvement, we conduct a comparative analysis with previous studies, confirming that the proposed preprocessing method outperforms previous preprocessing methods in terms of performance.

Ginseng-derived type I rhamnogalacturonan polysaccharide binds to galectin-8 and antagonizes its function

  • Yi Zheng;Yunlong Si;Xuejiao Xu;Hongming Gu;Zhen He;Zihan Zhao;Zhangkai Feng;Jiyong Su;Kevin H. Mayo;Yifa Zhou;Guihua Tai
    • Journal of Ginseng Research
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    • v.48 no.2
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    • pp.202-210
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    • 2024
  • Background: Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear. Methods: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis. Results: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar KD values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function. Conclusion: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.

A Comparative Study on Factors Influencing Residential Satisfaction by Types of Public Rental Housing (공공임대주택 유형별 주거만족도 영향요인 비교연구)

  • Mee-Jung Lee;Chan-Ho Kim;Chang-Soo Lee
    • Land and Housing Review
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    • v.15 no.1
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    • pp.39-55
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    • 2024
  • The aim of this study is to analyse housing satisfaction among residents of different types of public rental housing-permanent, national, and happy housing-following the integration of housing types upon the full-scale supply of integrated public rental housing. By identifying key factors that influence residential satisfaction, our goal is to inform the planning of public rental housing complexes and derive policy implications. The study focuses on analysing discrepancies in residential satisfaction among residents of different types of public rental housing and comparing the factors influencing this satisfaction. Microdata from the Ministry of Land, Infrastructure, and Transport's 'Housing Situation Survey' in 2021 is utilized for analysis, employing one-way ANOVA and binomial logistic regression methods. Empirical analysis reveals variations in residential satisfaction levels between residents of permanent and national rental housing, with national rental housing residents exhibiting higher satisfaction. The influencing factors of overall condition satisfaction are consistent for permanent and national rental residents but differ for happy housing residents. Additionally, the influencing factors of overall residential environmental satisfaction vary across all three housing types. Nonetheless, common factors across all types include housing noise and facility accessibility, highlighting their significance in complex planning. Subsequent studies may involve time series analysis to assess changes in influencing factors over time.

Modeling of Scattered Signal from Ship Wake and Experimental Verification (항적 산란신호의 모델링과 실험적 검증)

  • Ji, Yoon-Hee;Lee, Jae-Hoon;Kim, Jea-Soo;Kim, Jung-Hae;Kim, Woo-Shik;Choi, Sang-Moon
    • The Journal of the Acoustical Society of Korea
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    • v.28 no.1
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    • pp.10-18
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    • 2009
  • A moving surface vessel generates a ship wake which contains a cloud of micro-bubbles with radii ranging between $8{\sim}200{\mu}m$. Such micro-bubbles can be detected by active sonar system for more than ten minutes depending on the size and speed of the surface vessel. In this paper, a reverberation model for the ship wake is presented. The developed model consists of the acoustic scattering model due to the distribution of the micro-bubbles and the kinematic model for the moving active sonar. The acoustic scattering model is based on the volume integration, where the volume scattering strengths are obtained from the spatial distribution of micro-bubbles. Since the directivity and look-direction of active sonar are important factors for moving active sonar, the kinematic model utilizes the Euler transformation to obtain the relative motion between the global and local coordinates. In order to verify the developed model, a series of sea experiment was executed in September 2007 to obtain the spatial-temporal distribution of a bubble cloud, and analyzed to be compared with the simulation results.

Low Neutralizing Activities to the Omicron Subvariants BN.1 and XBB.1.5 of Sera From the Individuals Vaccinated With a BA.4/5-Containing Bivalent mRNA Vaccine

  • Eliel Nham;Jineui Kim;Jungmin Lee;Heedo Park;Jeonghun Kim;Sohyun Lee;Jaeuk Choi;Kyung Taek Kim;Jin Gu Yoon;Soon Young Hwang;Joon Young Song;Hee Jin Cheong;Woo Joo Kim;Man-Seong Park;Ji Yun Noh
    • IMMUNE NETWORK
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    • v.23 no.6
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    • pp.43.1-43.10
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    • 2023
  • The continuous emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants has provided insights for updating current coronavirus disease 2019 (COVID-19) vaccines. We examined the neutralizing activity of Abs induced by a BA.4/5-containing bivalent mRNA vaccine against Omicron subvariants BN.1 and XBB.1.5. We recruited 40 individuals who had received a monovalent COVID-19 booster dose after a primary series of COVID-19 vaccinations and will be vaccinated with a BA.4/5-containing bivalent vaccine. Sera were collected before vaccination, one month after, and three months after a bivalent booster. Neutralizing Ab (nAb) titers were measured against ancestral SARS-CoV-2 and Omicron subvariants BA.5, BN.1, and XBB.1.5. BA.4/5-containing bivalent vaccination significantly boosted nAb levels against both ancestral SARS-CoV-2 and Omicron subvariants. Participants with a history of SARS-CoV-2 infection had higher nAb titers against all examined strains than the infection-naïve group. NAb titers against BN.1 and XBB.1.5 were lower than those against the ancestral SARS-CoV-2 and BA.5 strains. These results suggest that COVID-19 vaccinations specifically targeting emerging Omicron subvariants, such as XBB.1.5, may be required to ensure better protection against SARS-CoV-2 infection, especially in high-risk groups.

The Relationship Between Entrepreneurial Competency and Entrepreneurial Intention of SME Workers: Focusing on the Mediating Effect of Start-Up Efficacy and Start-Up Mentor (중소기업 종사자의 창업역량과 창업의도 간의 영향 관계: 창업효능감과 창업멘토링의 매개효과 중심으로)

  • Oun Ju Lee
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.18 no.6
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    • pp.201-214
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    • 2023
  • This study attempted to analyze the impact of individual entrepreneurial capabilities on entrepreneurial intention targeting small and medium-sized business employees, and sought to confirm the mediating effect of entrepreneurial efficacy and entrepreneurial mentoring between entrepreneurial capabilities and entrepreneurial intention. The sub-variables of entrepreneurship competency were analyzed separately into creativity, problem solving, communication, and marketing. 368 questionnaires collected from employees at small and medium-sized manufacturing companies located across the country were used for empirical analysis. A parallel dual mediation model with no causal relationship between parameters was used for empirical analysis using SPSS v26.0 and PROCESS macro v4.2. As a result of the analysis, first, among the start-up competencies, creativity, communication, and marketing were confirmed to have a significant positive (+) effect on start-up efficacy. Second, among the start-up competencies, creativity, communication, and marketing were tested to have a significant positive influence on start-up mentoring. Third, both startup efficacy and startup mentoring were found to have a significant positive influence on startup intention. Fourth, among start-up capabilities, creativity and marketing were confirmed to have a significant positive (+) effect on start-up intention. Fifth, startup efficacy and startup mentoring were found to have a mediating effect on startup intention except for problem solving among startup competencies. As a result, it was confirmed that in order to strengthen the intention to start a business among small and medium-sized business employees, start-up efficacy and start-up mentoring are important factors, and that marketing and creativity have an important influence among individual start-up capabilities, so education and prior preparation for these are necessary. As follow-up research, it will be necessary to apply multivariate models, analyze time series data, research considering external environmental factors, and test the difference between start-up capabilities and performance considering detailed population characteristics.

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