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Isolation and Characterization of Cinnamoyl CoA Reductase Gene from Codonopsis lanceolata

  • Kim Ha-Na;In Jun-Gyo;Lee Eun-Kyung;Yoon Jae-Ho;Lee Mee-Sook;Bae Hyeun-Jong;Yang Deok-Chun
    • Plant Resources
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    • v.8 no.3
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    • pp.181-187
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    • 2005
  • A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

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Analysis of Optimal Sounding Signal Design in OFDM Systems

  • Jo, Junho;Oh, Janghoon;Choi, Seyeong
    • Journal of information and communication convergence engineering
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    • v.13 no.2
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    • pp.69-73
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    • 2015
  • We focus on a sounding signal design for single-input single-output orthogonal frequency division multiplexing (SISO-OFDM) systems. We show that the frequency spectrum of an optimum sounding signal has a constant magnitude across the frequency band for the cases with or without Doppler effects. Simulation results show that the designed optimum sounding signal outperforms random sounding signals and that the performance of a maximal-length shift register sequence is indistinguishable from that of the optimum sounding signal.

Application of rDNA-PCR Amplification and DGGE Fingerprinting for Detection of Microbial Diversity in a Malaysian Crude Oil

  • Liew, Pauline Woan Ying;Jong, Bor Chyan
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.815-820
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    • 2008
  • Two culture-independent methods, namely ribosomal DNA libraries and denaturing gradient gel electrophoresis (DGGE), were adopted to examine the microbial community of a Malaysian light crude oil. In this study, both 16S and 18S rDNAs were PCR-amplified from bulk DNA of crude oil samples, cloned, and sequenced. Analyses of restriction fragment length polymorphism (RFLP) and phylogenetics clustered the 16S and 18S rDNA sequences into seven and six groups, respectively. The ribosomal DNA sequences obtained showed sequence similarity between 90 to 100% to those available in the GenBank database. The closest relatives documented for the 16S rDNAs include member species of Thermoincola and Rhodopseudomonas, whereas the closest fungal relatives include Acremonium, Ceriporiopsis, Xeromyces, Lecythophora, and Candida. Others were affiliated to uncultured bacteria and uncultured ascomycete. The 16S rDNA library demonstrated predomination by a single uncultured bacterial type by >80% relative abundance. The predomination was confirmed by DGGE analysis.

The Optimal Preform Design for Automotive Differential Bevel Gear (자동차용 차동 베벨기어의 최적 예비성형체 설계)

  • 김병민;김동환;정구섭
    • Transactions of the Korean Society of Automotive Engineers
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    • v.12 no.1
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    • pp.184-189
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    • 2004
  • In this paper, the warm forging process sequence has been determined to manufacture the warm forged product for the precision bevel gear used as the differential gear unit of a commercial automobile. The preform shape of bevel gear influences the dimensional accuracy and stiffness of final product. The aspect ratio and chamfer length are considered as design parameters to achieve adequate metal distribution in the finish forging operation. Then the optimal conditions of design parameters have been selected by artificial neural network (ANN). Finally, to verify the optimal preform shape, the experiments of the warm forging of the bevel gear have been executed. The proposed method can give more systematic and economically feasible means for designing the preform shape in metal forming process.

BETTI NUMBERS OVER ARTINIAN LOCAL RINGS

  • Choi, Sangki
    • Bulletin of the Korean Mathematical Society
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    • v.31 no.1
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    • pp.35-44
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    • 1994
  • In this paper we study exponential growth of Betti numbers over artinian local rings. By the Change of Tor Formula the results in the paper extend to the asymptotic behavior of Betti numbers over Cohen-Macaulay local rings. Using the length function of an artinian ring we calculate an upper bound for the number of generators of modules, this is then used to maximize the number of generators of sygyzy modules. Finally, applying a filtration of an ideal, which we call a Loewy series of an ideal, we derive an invariant B(R) of an artinian local ring R, such that if B(R)>1, then the sequence $b^{R}$$_{i}$ (M) of Betti numbers is strictly increasing and has strong exponential growth for any finitely generated non-free R-module M (Theorem 2.7).).

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A study on randomization of tining space for concrete pavements (콘크리트 포장 타이닝 간격 랜덤화에 관한 연구)

  • Jung, Soon-Chul;Woo, Jong-Won;Kim, Yong-Soo;Lee, Jae-Eung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.11a
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    • pp.741-744
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    • 2004
  • In concrete pavements with uniform tining space, the whine noise which has high frequency spectrum related to the uniform tining space exist. In this paper, randomization procedure of tining space for concrete pavements is proposed. The proposed procedure developed based on the LCG(Linear Congruential Generator) algorithm. And the factor concerned in the procedure are drag span length, number of different tining space, ratio of each tining space, total tinings of each size, sequence of actual tining.

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A Statistical Thermodynamic Study on the Conformational Transition of Oligopeptide Multimer

  • Kim, Yong Gu;Park, Hyeong Seok
    • Bulletin of the Korean Chemical Society
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    • v.17 no.2
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    • pp.131-138
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    • 1996
  • The conformational transition of oligopeptide multimer,-(HPPHPPP)n-, is studied (H:hydrophobic amino acid, P:hydrophilic amino acid). The helix/coil transitions are detected in the multimer. The transition depends on the number of amino acid in the sequence, the concentration of the oligopeptide, and temperature which affects helix stability constant (${\xi}$) and hydrophobic interaction parameter (wj). In the thermodynamic equilibrium system jA${\rightarrow}$Aj (where A stands for oligopeptide monomer), Skolnick et al., explained helix/coil transition of dimer by the matrix method using Zimm-Bragg parameters ${\xi}$ and $\sigma$ (helix initiation constant). But the matrix method do not fully explain dangling H-bond effects which are important in oligopeptide systems. In this study we propose a general theory of conformational transitions of oligopeptides in which dimer, trimer, or higher multimer coexists. The partition of trimer is derived by using zipper model which account for dangling H-bond effects. The transitions of multimers which have cross-linked S-S bonds or long chains do not occur, because they keep always helical structures. The transitions due to the concentration of the oligopeptides are steeper than those due to the chain length or temperature.

Vibration and stability analyses of thick anisotropic composite plates by finite strip method

  • Akhras, G.;Cheung, M.S.;Li, W.
    • Structural Engineering and Mechanics
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    • v.3 no.1
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    • pp.49-60
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    • 1995
  • In the present study, a finite strip method for the vibration and stability analyses of anisotropic laminated composite plates is developed according to the higher-order shear deformation theory. This theory accounts for the parabolic distribution of the transverse shear strains through the thickness of the plate and for zero transverse shear stresses on the plate surfaces. In comparison with the finite strip method based on the first-order shear deformation theory, the present method gives improved results for very thick plates while using approximately the same number of degrees of freedom. It also eliminates the need for shear correction factors in calculating the transverse shear stiffness. A number of numerical examples are presented to show the effect of aspect ratio, length-to-thickness ratio, number of plies, fibre orientation and stacking sequence on the natural frequencies and critical buckling loads of simply supported rectangular cross-ply and arbitrary angle-ply composite laminates.

Development of Gait Recognition System (보행인식 시스템 개발)

  • Han, Y.H.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.8 no.2
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    • pp.133-138
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    • 2014
  • In this paper, a simple but efficient gait recognition method using spatial-temporal silhouette analysis is proposed. For each image sequence, a background subtraction algorithm and a PBAS(pixel based adaptive segmenter) procedure are first used to segment the moving silhouettes of a walking figure. Then, to identify people, the step count and stride length of walking figure is obtained in silhouette images. Experimental results on a CASIA dataset including 124 subjects demonstrate the validity of the proposed method. Also, the proposed system are believed to have a sufficient feasibility for the application to gait recognition.

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