• 한국정보전자통신기술학회논문지
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• 제10권4호
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• pp.325-332
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• 2017

본 논문은 프로토콜 구현물이 프로토콜의 사양에 대한 적합성을 시험하기 위한 시험항목 생성방법들에 대하여 비교분석 한다. 대표적인 방법들인 천이 순회, 구별 시퀀스, 특징화 시퀀스, 유일 입출력 시퀀스와 변형된 이들 방법들을 분석하고, 유한 상태 기계 모델에 적용한 위의 방법들의 시험항목 길이를 비교 및 분석 한다. 결론에서는 프로토콜 적합성시험을 위한 시험항목 생성방법들에 대한 핵심적이고 분석적인 이슈 사안들을 다음과 같이 제시한다. 천이 순회 방법은 최단의 시험 항목을 생성하지만 최악의 오류 검출 성능을 제공한다. 특징화 시퀀스 방법은 완벽한 오류 검출 성능을 제공하지만 상대적으로 최장의 시험 항목을 생성한다. 구별 시퀀스 방법의 문제점은 이 구별 시퀀스가 항상 존재하지는 않는다는 것이다. 유일 입출력 시퀀스 방법이 비교적 폭넓게 적용될 수 있지만 구별 시퀀스 방법과 동일한 오류 검출 성능을 제공하지 못한다는 문제점이 있다.

• 융합신호처리학회논문지
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• 제13권4호
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• pp.194-200
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• 2012

본 논문에서는 S.Bostas와 V.Kumar[7]에 의하여 제안되고 $GF(2^n)$에서 정의되는 부호계열 발생알고리즘을 분석하고, 길이 n인 이진벡터로 이루어지는 벡터공간 $F_2$으로부터, 두 원소로 정의되는 공간 $F_2$로 사상할 수 있는 부울함수를 이용하여 발생기 구성 함수를 도출하였다. 차수 n=5와 n=7인 두 종류의 최소 다항식을 이용한 피드벡 쉬프트레지스터를 기반으로 Trace 함수로부터 부호계열 발생기 구성 부울함수를 도출하고 발생기를 설계 구성하였으며 이를 이용하여 두 종류의 부호계열 군을 발생하였다. 발생된 부호계열의 주기는 각각 31과 127로서 주기 $L=2^n-1$을 만족하고 ${\tau}=0$을 제외한 자기상관함수 값과 상호상관함수 값이 각각 {-9, -1, 7}과 {-17, -1, 15}로서 상관함수 값 $R_{i,j}({\tau})=\{-2^{(n+1)/2}-1,-1,2^{(n+1)/2}-1\}$의 특성을 만족하였다. 이 결과로부터 부울함수를 이용한 부호계열 발생기 설계와 구성이 타당함을 확인하였다.

• BMB Reports
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• 제37권5호
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• pp.574-581
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• 2004

The full-length cDNA of the lumbrokinase fraction 6 (F6) protease gene of Lumbricus rubellus was amplified using an mRNA template, sequenced and expressed in E. coli cells. The F6 protease gene consisted of pro- and mature sequences by gene sequence analysis, and the protease was translated and modified into active mature polypeptide by N-terminal amino acid sequence analysis of the F6 protease. The pro-region of F6 protease consisted of the 44 residues from methionine-1 to lysine-44, and the mature polypeptide sequence (239 amino acid residues and one stop codon; 720 bp) started from isoleucine-45 and continued to the terminal residue. F6 protease gene clones having pro-mature sequence and mature sequence produced inclusion bodies in E. coli cells. When inclusion bodies were orally administrated rats, generated thrombus weight in the rat' venous was reduced by approximately 60% versus controls. When the inclusion bodies were solubilized in pepsin and/or trypsin solutions, the solubilized enzymes showed hemolytic activity in vitro. It was concluded the F6 protease has hemolytic activity, and that it is composed of pro- and mature regions.

• Ocean Science Journal
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• 제40권3호
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• pp.155-166
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• 2005

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellate Alexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report in Alexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison with Prorocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton, A. tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities of Alexandrium genus, particularly of the tamarensis complex.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.147.2-148
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• 2003

Complete nucleotide sequence of genome RNA of a Korean isolate of Pepper mottle virus (PepMoV-Vb) from field-collected diseased paprika (Capsicum annuum var grossum) was determined in this study. Symptoms of isolates of PepMoV were divided largely into two groups, vein banding (Vb) and vein clearing (Vc) patterns. PepMoV-Vb RNA consists of 9,640 nucleotides excluding the poly(A) tail. A single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3024 amino acids which is typical of the Potyvirus genus. The complete nucleotide sequence and coding regions of PepMoV-Vb were compared to that of 11 potyviruses within the genus Potyvirus. The overall nucleotide sequence identity was 94.7 and 94.1％ identical to PepMoV-C and PepMoV-FL, respectively. Full-length cDNAs of PepMoV-Vbl were synthesized from purified viral RNAs by RT-PCR and their genome structure was analysed by RFLP analysis. This is the first report on complete nucleotide sequence of PepMoV isolated from paprika in Korea.

• 한국경영과학회지
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• 제16권2호
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• pp.36-48
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• 1991

In this study, an efficient algorithm is developed to compute steady state probabilities for the following M$_{x}$G/1 server vacation system under control-operating poligy: At the end of a busy period, the server takes a sequence of vacations, each for a random amount of time. At the end of each vacation, he inspects the length of the queue. If the queue length at this time is equal to or greater than a prespecified threshold value r, he begins to serve the queue until it is empty.y.

• 한국동물학회지
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• 제1권2호
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• pp.17-24
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• 1958

Since Kesser first described in 1934 the functional change of the autonomic nervous system caused by certain visible rays many researchers have unanimously approved that animals flashed with a red visible ray develop parasympathicotony while those flashed with a blue visible ray develop sympathicotony. On the other hand through studies made by our colleagues it is now well known that the inner-movement of the retinal pigments of frogs is stimulated in sympathicotony and is in reverse inhibited in parasimpathicotony. It is almost evident that the mechanism by which the inner-movement of the retinal pigments is due to sympathicotony derived from the excessive secretion of adrenalin. In addition , through may recent experiments on the pharmacological action of various medicines on the retinal pigments reaction of tadpoles , ranging from the excessive secretion of adrenalin. In addition , through my recent experiments on the pharmacologtical action of various medicines on the retinal pigments reaction of tadploes, raging from every developmental stage , Ifound that the movement of the retinal pigments by adrenalin is predominant in the earlier developmental stages of taopoles around 11 mm of body length, whereas other medicines fail to give any responce to the retinal pigments in such an earlier stage. When tadpoles grown to body length of 15-16m the retinal pigments move to the complete light position while kept in adrenalin solution. Based on these facts it might be well to consider that if tadpoles were grown under the visible rays for a given period, they might show a functional change of the autonomic nervous system and thereby cause of certain change in the physciological phases of the retinal reaction. Experiments were undertaken to find this matter and also to discover the simultaneous effects of the visible radiations on the developmental process of tadpoles. The results summarized as follows ; 1. The longest wave of visible rav has an effective reaction on the growth of body length of taopoles, while the shortest wave of visible ray causes the same for the metamorphoric differentiation of tadpoles. 2. When keeping two groups of tadpoles the first group of 15 mm body length grown for the period of one week and the latter group of 20 mm body length grown for two weeks under the various visible rays. swimming in adrenalin solution, the inner-movement of the retinal pigment occurs in both groups. The movement of pigments of the first group is accelerated in a sequence of blue ray \ulcorner green ray > brown ray> red ray, and that of the latter group is also accelerated in a sequence of blue ray>green ray > brown ray and red ray. 3. When keeping tow groups of tadpoles, the first group of 20 mm body length grown for the period of two weeks, the latter group of 25 m body length grown for three wheeks, under the various visible rays in sunlight, the inner-movement of the retinal pigments occurs in both groups. The movement of pigments of the first group is accelerated in a sequence of blue ray> green ray>brown ray and red ray, and that of the latter group is also accelerated in a sequence of blue ray > brow ray>red ray. 4. In order words, there facts manifest that tadpoles grown under the various visible rays reveal functional disturbances of the autonomic nervous system, at the time of 15 mm body length by adrenalin solution, which is a unique indicator illustrating the status of sympathicotony, and at the time of 20 mm body length by sunlight. This means that the longest visible ray cause sympathicotony, while the shortest visible ray causes parasympathicotony.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.345-348
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• 2015

Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

• Journal of Plant Biology
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• 제37권2호
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• pp.167-173
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• 1994

Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

• Journal of the korean society of oceanography
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• 제35권3호
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• pp.158-160
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• 2000

The relativity of four isolates of C. polykrikoides was determined by comparative sequence analysis based on direct sequencing of PCR amplified ribosomal DNA (the internal transcribed spacer region and the 5.8S rDNA). Sequence comparisons indicated that four isolates had the same nucleotide sites in the ITS regions, as well as a total of 585 nucleotide length and 100% homology. The molecular data revealed that C. polykrikoides in Korean coastal waters show no genetical difference.