• Journal of Life Science
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• v.21 no.3
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• pp.459-463
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• 2011

The genome of the rhesus monkey has diverged as an average sequence identity of ~93%. The rhesus monkey has been widely used as a non-human primate in the field of biomedical and evolutional research. Insertion of transposable elements (TEs) induced several events such as transcriptional diversity and different expression in host genes. In this study, 112 transcripts were identified from a full-length cDNA library of brain tissues of the rhesus monkey. One transcript (R54) showed a different expression pattern between human and rhesus monkey tissues. This phenomenon can be an explanation that R54 transcript was acquired by splicing a donor site derived from exonization of the L2A element. Therefore, integration of TEs during primate radiation could contribute to transcriptional diversity and gene regulation.

• Fisheries and Aquatic Sciences
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• v.11 no.2
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• pp.88-97
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• 2008

Lipoproteins are complexes of lipids and specific apolipoproteins that are involved in lipid transport and redistribution among various tissues. In this study, we isolated full-length apolipoprotein cDNA sequences encoding apolipoprotein A-I (apoA-I), apoE, apoC-II, and apo-14 kDa in barred knifejaw, Oplegnathus fasciatus. In addition, we reconstructed phylogenetic trees and investigated mRNA tissue distributions. Alignment analyses of amino acid sequences revealed that secondary structures of the polypeptides apoA-I, apoE, and apoC-II in barred knifejaw are well conserved with their teleostean and mammalian counterparts in terms of characteristic tandem repetitive units forming amphipathic ${\alpha}$-helices. Both the sequence alignment data and cleavage sites of apo-14 kDa indicated a clear differentiation between Percomorpha and Cypriniformes. Meanwhile, the phylogenetic trees of apolipoprotein sub-families suggested that the common ancestor prior to the split of the Actinopterygii (ray-finned fishes) and Sarcopterygii (tetrapods) would have possessed the primordial protein-encoding genes. Tissue distribution of each apolipoprotein transcript determined by semi-quantitative RTPCR showed that barred knifejaw apoA-I transcripts were more or less ubiquitously expressed in the liver, intestines, brain, muscle, spleen, and kidney. The most striking difference from previous observations on barred knifejaw was the ubiquitous expression of apoE across all somatic tissues. Barred knifejaw apoC-II showed tissue-specific expression in the liver and intestines, while the liver and brain were the major sites of apo-14kDa mRNA synthesis.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.2
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• pp.292-303
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• 1998

The arbitrary primer polymerase chain reaction(AP-PCR) and Southern blot restriction fragment length polymorphism(RFLP) were used to genotype the cariogenic pathogen S. mutans in children. Following the morphologic chracteristics of colony on selective medium for S. mutans, total genomic DNA from 155 strains was extracted by conventional methods. Among 155 strains, 143 strains (92.3%) were confirmed S. mutans by PCR with dexA gene and 114 strains were used in this study. Three random sequence 10-base oligonucleotide primers were chosen for AP-PCR. The amplified DNA products were separated electrophoretically in a 2% agarose gel containing ethidium bromide and the banding patterns were compared among different strains. For RFLP analysis, DNA was digested with EcoRI and BamHI, separated on a 0.7 % agarose gel and transferred to a nylon membrane. The membrane was probed with a previously characterised 1.6 kilobases (kb) DNA fragment cloned from gtf B gene of S. mutans. The probe was labeled with isotope[$^{32}P-{\alpha}CTP$], and hybridized fragments were detected with intensifying screen. AP-PCR produced 4-8 DNA bands in the 0.25-10 kb regions and distinguished 9, 10 or 12 genotypes, depending on the specific primer used. Southern blot RFLP analysis revealed 2 hybridization patterns consisting of 1 DNA fragments 450, 500 bp. These results indicate that AP-PCR is more discriminative method for genotyping of S. mutans.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.4
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• pp.914-925
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• 1998

This paper considers the evaluation of the hybrid acquistion perdformance for the pilot signal in the direct sequence code division multiple access(DS/CDMA) forward link. the hybrid acquisition is introduced by the combination of two schemes, the parallel and serial acquisions. The mean acquisition time of the proposed scheme is derived to consider both the best case(the correct code-phase offsets are included i one subset) and the worst case(the correct code-phase offsets exist at the boundary of two subsets), which are cause by the distribution of the correct code-phase offsets in the subset. Expressions for the detection, false alarm, and miss probabilities are derived for the case of multiple correct code-phase offsets and multipath Rayleigh fading channel. Numerical results present the hybrid acquistion performance with repect to design parameters such as postdetectio integration length in the search and verification modes, subset size, and number of I/Q noncoherent correlators, and compare the hybrid acquistion with the parallel acquistion in terms of the minimum acquistion time under the same hardware complexity.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.9B
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• pp.914-922
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• 2009

In this paper, we propose a matrix based real-time pattern matching method, called MDPI, for real-time intrusion detection on several Gbps network traffic. Particularly, in order to minimize a kind of overhead caused by buffering, reordering, and reassembling under the circumstance where the incoming packet sequence is disrupted, MDPI adopts independent partial matching in the case dealing with pattern matching matrix. Consequently, we achieved the performance improvement of the amount of 61% and 50% with respect to TCAM method efficiency through several experiments where the average length of the Snort rule set was maintained as 9 bytes, and w=4 bytes and w=8bytes were assigned, respectively, Moreover, we observed the pattern scan speed of MDPI was 10.941Gbps and the consumption of hardware resource was 5.79LC/Char in the pattern classification of MDPI. This means that MDPI provides the optimal performance compared to hardware complexity. Therefore, by decreasing the hardware cost came from the increased TCAM memory efficiency, MDPI is proven the cost effective high performance intrusion detection technique.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.9 no.2
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• pp.191-198
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• 1998

The error are equation of DC-CDMA/MDPSK signal has been derived in m-distribution and Rician fading channels. Predeteception multipath MRC(Maximal Ratio Combining) diversity technique is employed for improving the bit error rate performance. The suitability of modeling a Rician fading environment by properly chosen m-distribution model is examined. Using the derived equation the error performance has been evaluated and shown in figures as a function of PN code sequence length(N), user number(U), multipath number(P), fading index(m), Rician factor(K), number of diversity branches(L) and ($E_b/N_o$). The results show that the error performance in Rician fading agrees well with that in m-distribution fading as fading becomes weak and as user number(U) increases and as multipath number(P) increases and diversity number(L) increases.

• Proceedings of the KIEE Conference
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• 2003.11b
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• pp.295-298
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• 2003

In this paper, a novel maximum a posterion (MAP) estimation for the channel decoding of H.264 codes in the presence of transmission error is presented. Arithmetic codes with a forbidden symbol and trellis search techniques are employed in order to estimate the best transmitted. And, there has been growing interest of communication, the research about transmission of exact data is increasing. Unlike the case of voice transmission, noise has a fatal effect on the image transmission. The reason is that video coding standards have used the variable length coding. So, only one bit error affects the all video data compressed before resynchronization. For reasons of that, channel needs the channel codec, which is robust to channel error. But, usual channel decoder corrects the error only by channel error probability. So, designing source codec and channel codec, Instead of separating them, it is tried to combine them jointly. And many researches used the information of source redundancy In received data. But, these methods do not match to the video coding standards, because video ceding standards use not only one symbol but also many symbols in same data sequence. In this thesis, We try to design combined source-channel codec that is compatible with video coding standards. This MAP decoder is proposed by adding semantic structure and semantic constraint of video coding standards to the method using redundancy of the MAP decoders proposed previously. Then, We get the better performance than usual channel coder's.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1373-1380
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• 2018

Objective: This study was designed to investigate the effects of temperature and storage time on the evolution of bacterial communities in swine manure. Methods: Manure was stored at $-20^{\circ}C$, $4^{\circ}C$, $20^{\circ}C$, or $37^{\circ}C$ and sampled at 7-day intervals over 28 days of storage, for a total of 5 time points. To assess the bacterial species present, 16S ribosomal RNA gene sequences were analyzed using pyrosequencing. Results: After normalization, 113,934 sequence reads were obtained, with an average length of $466.6{\pm}4.4bp$. The diversity indices of the communities reduced as temperature and storage time increased, and the slopes of rarefaction curves decreased from the second week in samples stored at $-20^{\circ}C$ and $4^{\circ}C$. These results indicate that the richness of the bacterial community in the manure reduced as temperature and storage time increased. Firmicutes were the dominant phylum in all samples examined, ranging from 89.3% to 98.8% of total reads, followed by Actinobacteria, which accounted for 0.6% to 7.9%. A change in community composition was observed in samples stored at $37^{\circ}C$ during the first 7 days, indicating that temperature plays an important role in determining the microbiota of swine manure. Clostridium, Turicibacter, Streptococcus, and Lactobacillus within Firmicutes, and Corynebacterium within Actinobacteria were the most dominant genera in fresh manure and all stored samples. Conclusion: Based on our findings, we propose Clostridium as an indicator genus of swine manure decomposition in an anaerobic environment. The proportions of dominant genera changed in samples stored at $20^{\circ}C$ and $37^{\circ}C$ during the fourth week. Based on these results, it was concluded that the microbial communities of swine manure change rapidly as storage time and temperature increase.

• Mycobiology
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• v.29 no.3
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• Korean Journal of Veterinary Service
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• v.30 no.3
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• pp.393-406
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• 2007

Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7 kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism, The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.