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Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

  • Karabag, Kemal;Balcioglu, Murat Soner;Karli, Taki;Alkan, Sezai
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.12
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    • pp.1696-1701
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    • 2016
  • Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed ($H_o$) and expected ($H_e$) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, $H_o$ and $H_e$ were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative ($PIC{\geq}0.50$). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required.

An Experimental Study of Aerodynamic Characteristics on a Projectile with Counter-Rotating Head Installed Fins (조종면이 장착된 회전하는 발사체에서의 공력특성 분석에 관한 실험적 연구)

  • Park, Young-Ha;Je, Sang-Eon;Cho, Soo-Yong
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.5
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    • pp.357-365
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    • 2013
  • In this study, forces and moments were measured on a projectile which consisted of a missile configuration body(shell) and a head installed control fins. The shell and the head were separated each other and the shell was rotated by an electric motor. The head rotated reversely against the rotational direction of the shell. The rotational force on the head was obtained from a couple of fixed fins of which angular displacement were set to the rotational direction equally. The air velocity was 40m/s on the experiment and the Reynolds number based on the diameter of head was $1.3{\times}10^5$. The other couple of fins were used to control the position and direction of the projectile by changing the angular displacement. From this experiment, the variation of force and moment were measured on the rotating projectile, and the effective amplitude and frequency were obtained through the FFT analysis.

A Study on Using the Interior Finishing Material using Regulation at Multiple-using Businesses (다중이용업소 실내마감재료 사용기준에 관한 연구)

  • Kim, Yong-Sung;Cho, Sung-O
    • Korean Institute of Interior Design Journal
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    • v.22 no.5
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    • pp.216-224
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    • 2013
  • There are the relevant provisions that apply to building regulations about the Building codes, Fire Protection laws, and more Multi-using interior finish materials businesses etc. The differences between these regulations are as follows: The Building codes is defined the main use of the building according to facilities to used to set the scale by the use of the floor space, On the other hand, Fire Protection laws are the basic purpose and the act of using the architectural space(or sales) by separating object by fire. And Multiple-using Businesses Facilities consist of two regulations engaged in the kind of law and the size of the floor area. One building can not be based on operating facilities and together in Multiple use businesses Law. As such, the Buildings are applied to the Building codes, Fire Protection laws, such as Multi-using Businesses Facilities interior construction industry due to the structural differences in the position of the designer feeling a lot of difficulties and confusion. The Multi-use businesses Facilities engage in the kind of law, the size of the floor area and seating capacity. These can not be based on operating facilities, and consists of two regulations together in one building. This study is purposed to help improving the finish of the interior architecture that is related to the Building code regulations, Fire Protection laws, Multi-using Facilities interior finishing material relevant with the provisions from the law through analyze the case of developed countries, and the current Multiple use Businesses Facilities research range from the law of multiple-using facilities, interior finishing, interior architecture for supervision reasonably and compensation for the interior design. So, Result is as follows: First, interior decorations can be separated the fixed material and the moveable things. second, It should be included multi-using Business though small size business space at the basement floor. third, It should be established statute law that design and construction responsibility. forth, re-testing fire resist performance after some period. and finally, it need to mitigate regulation where be installed sprinkler.

Design of Switching Fabric Supporting Variable Length Packets (가변 길이 패킷을 지원하는 스위칭 패브릭의 설계)

  • Ryu, Kyoung-Sook;Kim, Mu-Sung;Choe, Byeong-Seog
    • Journal of KIISE:Computing Practices and Letters
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    • v.14 no.3
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    • pp.311-315
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    • 2008
  • The switching fabric used to make high speed switching for packet transfer between input and output interface in recent internet environments. Without making any changes in order to remain ATM switching fabric, the existing structures should split/reassemble a packet to certain size, set aside cross-point buffer and will put loads on the system. In this paper, we proposed a new switch architecture, which has separated data memory plane and switching plane packet data will be stored on the separate memory structure and simultaneously only the part of the memory address pointers can pass the switching fabric. The small mini packets which have address pointer and basic information would be passed through the switching fabric. It is possible to achieve the remarkable switching performance than other switch fabrics with contending variable length packets.

A Study of application on the Figurative Aesthetics of Oriental Painting (동양회화(東洋繪畵)에서 형상(形象)의 심미작용(審美作用))

  • Jeong Jin-Ryong
    • Journal of Science of Art and Design
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    • v.8
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    • pp.211-239
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    • 2005
  • The purpose of this thesis is to explore a common characteristic of the diverse oriental images mainly from the figurative point of view, and apply it to the contemporary expressions and methods of painting. To do that, I separated the concept of figure into two factors - form and image - and then approached and solved the problems of figure by explaining the two factors respectively. Separating form from image is neither to set two factors against each other nor to isolate any of those values. It is worth dividing the form and the image not only because the image of the oriental painting has a figurative value constituted from an external frame of the form, but also because it has a close connection with the meaning that the form holds inside. These are the reasons why I conceptualize the individual language of form and image. Furthermore, I attempted to investigate how those two factors and mutual relations between them make real figurative images under the principle of harmony and coexistence. The theme of this thesis - the figurative aesthetics and its contemporaneity in the oriental paintings is analyzed based on the understanding of 'figure' within the framework of oriental paintings. A research of image from the figurative point of view is valuable in that it criticizes the past method of thinking, and proposes an alternative formula for new way of thinking. In this regard, I indicated the limit of the theory of form-and-spirit that has been one of the most important conceptual theories . Instead, I' accessed to various aesthetic concepts - that are created in the course of image formulation - from the actual figurative point of view, and then even interpreted it as a figurative value of the oriental paintings and it's application within the specific and practical scope. During the course of research, I explored the conceptual elements of the tradition and the principle of figuration. I expect this study to make a footstone for bringing traditional aesthetics to the contemporary context of oriental painting.

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Simultaneous Determination of Six Components in the Traditional Herbal Medicine 'Oryeongsan' by HPLC-DAD and LC-MS/MS

  • Lee, Jiwoo;Weon, Jin Bae;Lee, Bohyoung;Yun, Bo-Ra;Eom, Min Rye;Ma, Choong Je
    • Natural Product Sciences
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    • v.19 no.1
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    • pp.20-27
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    • 2013
  • A simple high performance liquid chromatography - diode array detector (HPLC-DAD) method has been developed and validated for simultaneous determination of the six components (cinnamic acid, cinnamaldehyde, eugenol, atractylenolide I, atractylenolide III, and ergosterol) in Oryeongsan. In addition, identification of six marker compounds was conducted by a LC-MS/MS analysis. The six compounds in Oryeongsan were separated on Shishedo $C_{18}$ column (5 ${\mu}m$, $4.6{\times}250$ mm) at a column temperature of $30^{\circ}C$. The mobile phase was a mixture of 0.1% trifluoroacetic acid (TFA) water and acetonitrile employing gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was set at 205, 250, 280, and 330 nm. The developed method had good linearity ($R^2$ > 0.9997) and the limit of detection (LOD) and limit of quantification (LOQ) were observed within the ranges 0.01~0.15 and 0.05~0.45 ${\mu}g/mL$, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were indicated that less than 3% and 90.31~103.31% of accuracy. The results of recovery test were 90.56~106.72% and RSD range was measured from 0.84 to 2.95%. In conclusion, this HPLC-DAD method has been successfully applied to the simultaneous determination of six compounds in Oryeongsan samples.

Effect of Lubricant with Nanodiamond Particles in Sliding Friction

  • Adzaman, M.H.;Rahman, A.;Lee, Y.Z.;Kim, S.S.
    • Tribology and Lubricants
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    • v.31 no.4
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    • pp.183-188
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    • 2015
  • This paper presents the experimental effects of lubricant with nanodiamond particles in sliding friction. In order to improve the performance of lubricants many additives are used, such as MoS2, cadmium chloride, indium, sulfides, and phosphides. These additives are harmful to human health and to the environment, so alternatives are necessary. One such alternative is nanodiamond powder, which has a large surface area. In order to investigate the effect of nanodiamonds in lubricants under sliding friction, they are dispersed in the lubricant at a variety of concentrations (0 wt%, 0.1 wt%, 0.3 wt%, 0.5 wt%, and 1 wt%) using the matrix synthesis method. Friction and wear tests are performed according to the ASTM G99 method using a pin-on-disc tester at room temperature. The specimens used in this experiment are AISI 52100 ball bearings and AISI 1020 steel discs. During the test, lubricant mixed with nanodiamond is supplied constantly to keep the two bodies separated by a lubricant film. To maintain boundary lubrication, the speed is set to 0.18 m/s and a load of 294 N is applied to the disc through the pin. Results are recorded by using workbench software over the test duration of 10 minutes. Experimental results show that when the concentration of nanodiamond increases, the coefficient of friction decreases. However, above a nanodiamond concentration of 0.5 wt%, both the coefficient of friction and wear volume increase. From this experiment, the optimum concentration of nanodiamond showing a minimum coefficient of friction of 0.09 and minimum wear volume of 0.82 nm2 was 0.5 wt%.

Analysis of Rutin Contents in Buckwheat and Buckwheat Foods (메밀 및 메밀식품에서의 루틴함량의 분석)

  • Maeng, Young-Sun;Park, Hye-Kyung;Kwon, Tae-Bong
    • Korean Journal of Food Science and Technology
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    • v.22 no.7
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    • pp.732-737
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    • 1990
  • In this study, the rutin contents in buckwheat and buckwheat foods were determined. Rutin in buckwheat and buckwheat foods was extracted with methanol and separated by High Performance Liquid Chromatography(HPLC) equipped with Lambda-Max Model 481 detector set at 355 nm using a ${\mu}$ Bondapak $C_{18}$ column and a 2.5% acetic acid:methanol:acetonitrile (35:5:10, v:v:v) solvent. There were differences in the rutin contents among the different species of buckwheat. The rutin contents in buckwheat groats were ranged from 8.84 mg to 24.77 mg/100g. The rutin contents in commercial buckwheat groats and flours were ranged from 15.04 mg to 20.92 mg/100g. The rutin contents in commercial dried buckwheat noodles, steamed buckwheat noodles, and buckwheat cookies were ranged from 1.76 mg to 10.84 mg/100g.

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A Study on Guidelines of Home Modification for Low-Income Elderly (저소득층 고령자를 위한 주택개조 가이드라인 연구)

  • Lee, Yeun-Sook;Lim, Yea-Ji;Kim, Hyun-Jung;Ahn, Chang-Hun
    • Journal of the Korean housing association
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    • v.26 no.3
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    • pp.65-76
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    • 2015
  • With a rapidly aging population, the proportion of elderly households with low income has been increasing. Despite the poor housing environment, it is not easy to improve their housing environment due to the high cost of modification. However, as many elderly want to keep living in their current houses, it is urgent to improve their housing environment. The purpose of this study was to develop the guidelines on home modification for low-income elderly. This study set the scope of home modification categories through literature analysis in advance to develop the guidelines. Based on the literature analysis and small group workshops, the primary and secondary guidelines were derived and a total of 169 final guidelines were produced based on the scope of home modification categories. Those guidelines were composed of the categories by space, divided into mandatory and recommended by details. Those guidelines proposed in this study were classified and composed under the objective standards, so that they were systemic and objective based on the verification of experts. They are considered to get closer to the user's demand on the basis of the demand of low income elderly for home modification and the improvement categories under the system to support home modification for low income elderly at home and abroad. In addition, as the standards to apply each guideline, separated into mandatory and recommended, was suggested, those guidelines may help expand the scope of improvement under the economic conditions for home modification.

Micro-gap DBD Plasma and Its Applications

  • Zhang, Zhitao;Liu, Cheng;Bai, Mindi;Yang, Bo;Mao, Chengqi
    • Journal of the Speleological Society of Korea
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    • no.76
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    • pp.37-42
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    • 2006
  • The Dielectric Barrier Discharge (DBD) is a nonequilibrium gas discharge that is generated in the space between two electrodes, which are separated by an insulating dielectric layer. The dielectric layer can be put on either of the two electrodes or be inserted in the space between two electrodes. If an AC or pulse high voltage is applied to the electrodes that is operated at applied frequency from 50Hz to several MHz and applied voltages from a few to a few tens of kilovolts rms, the breakdown can occur in working gas, resulting in large numbers of micro-discharges across the gap, the gas discharge is the so called DBD. Compared with most other means for nonequilibrium discharges, the main advantage of the DBD is that active species for chemical reaction can be produced at low temperature and atmospheric pressure without the vacuum set up, it also presents many unique physical and chemical process including light, heat, sound and electricity. This has led to a number of important applications such as ozone synthesizing, UV lamp house, CO2 lasers, et al. In recent years, due to its potential applications in plasma chemistry, semiconductor etching, pollution control, nanometer material and large area flat plasma display panels, DBD has received intensive attention from many researchers and is becoming a hot topic in the field of non-thermal plasma.