• Animal cells and systems
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• 제15권4호
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• pp.315-324
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• 2011

The actiniarian sea anemone, Entacmaea quadricolor, and the scleractinian coral, Alveopora japonica, host symbiotic dinoflagellates belonging to the genus Symbiodinium (Freudenthal). We studied the host-symbiont specificity of these two anthozoan hosts in the northwestern Pacific Ocean. Symbionts within the two hosts were identified using partial large subunit (LSU) ribosomal DNA (rDNA) and complete internal transcribed spacers (ITS) 1 rDNA regions. The host, E. quadricolor, was identified using the partial LSU rDNA molecular marker. Genetic analysis showed that E. quadricolor only harbors dinoflagellates belonging to subclade C1/3 of the genus Symbiodinium. Moreover, no genetic variation was detected among the symbionts of E. quadricolor within the study region (Korea and Japan), even though the two distant sites were separated by more than 1000 km, at collection depths of 1 m in shallow and 13-16 m in deep water. Whilst scleractinian corals host multiple Symbiodinium clades in tropical waters, A. japonica, sampled over a wide geographical range (800 km) within the study region, only hosts Symbiodinium sp. clade F3. The high specificity of endosymbionts in E. quadricolor and A. japonica within the northwestern Pacific Ocean could be accounted for because symbiotic dinoflagellates within the host anemones appear to be acquired maternally, and the Kuroshio Current might affect the marine biota of the northwestern Pacific. However, the consistency of the symbiotic relationships between these two anthozoan hosts and their endosymbionts could change after climate change, so this symbiotic specificity should be monitored.

• Journal of Dairy Science and Biotechnology
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• 제14권1호
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• pp.43-56
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• 1996

The world dairy industry has been driving their dairy policy on the basis of the self-supportihg principle. With progressing the GATT negotiation, the objective production amount has been reduced to maintain the optimum level, which decreasing the extra supply and over-stock before the U.R talks. The Oceania countries, where has the price competition capability, are continually increasing the milk production amount. Even through the USA is trying to increase their production amount with new export policy, the U.S.S.R collapse-down caused to decrease the milk production in world-wide basis. Because the U.S.S.R produced the highest amount of milk in the world. Moreover Europe Union countries, which recorded the half amount of dairy products in the world was decreasing their milk production. Therefore, about 4% of shortage is encountering at major dairy production countries in the standpoint of supply and demand. The drastic increasement of foreign dairy product import affects the domestic dairy industry with raw milk replacement and new dairy food demands creation since 1995, the initial period of free import system. Now, the domestic dairy industry are facing the inevitable problems such as the correction of producting and processing system. First of all, our dairy industry should resettle down the dairy production structure on the basis of self-supply of raw milk and offer the accurate dairy food informations. We still need to improve the raw milk quality. Also, more efforts are needed to have proper raw milk price policy. General domestic policies including the settlement of raw milk supply and demand, the reliability of inspection system in raw milk, ideal raw milk price system, centralization of raw milk collection, specially separated dairy organization should be improved legally in our dairy industry.

• Parasites, Hosts and Diseases
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• 제21권2호
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• pp.150-156
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• 1983

1975년부터 1983년까지 치료를 위해 서울의대 기생충학교실로 의뢰된 광절열두조충 충란양성자 5명에 대하여 bithionol, niclosamide 또는 praziquantel을 투여 하고 하제를 사용하여 충체를 얻은바 Diphyllobothrium latum으로 동정되었다. 충자는 서울 및 전남 완도군에 거주하고 있는 10~64세 남(4명) 여(1명)로 그중 2례는 농어를 생식한 경험이 있으며 나머지 3례는 바다생선회를 생식하였으나 그 종류를 기억할 수 없다고 한다. 또 그중 3례는 심한 복통과 소화기증상을 경험하였고, 2례는 편절이 자연배출된 일이 있다고 하나 빈혈소견은 전례에서 관찰되지 않았다. 대편검사에서 발견된 충란은 명 열별로 크기 평균 $59~67{\times} 41~48{\mu}m$이고 황갈색 유란원형 또는 타원형이 있으며 operculum과 abopercular protuberance가 뚜렷하였다. 충체는 4례에서 두절을 포함한 걸이 320~680cm의 완전한 것이 배출되었고 l례에서는 투약후 충체 배출여부를 확인하지 못하고 자연배출된 길이 30cm정도의 체절을 동정에 이용하였다. 특징적인 편절의 형태인 rosette형 자궁, vaginal pore가 cirrus sac에 포함되어 개구된 점, 재정과 cirrus sac의 분리 등으로 보아 Diphyllobothrium latum으로 동정되었다. 본 5례는 우리나라에서 1971년 이후 충체가 확인된 6례에 이어 제 7례-제11례의 인체기생례가 된다.

• 한국환경과학회지
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• 제15권7호
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• pp.607-614
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• 2006

A survey of middle school students and teachers in Mokpo city has been done to understand the perception of environment of students and the present situation of environmental education in middle school. Only 2 of the 14 middle schools have chosen the environmental education course as a required subject. The majority of middle-school students in Mokpo city have learned the environmental education as a subordinate course included in the other subjects such as science, social science or technology. The environmental education in school has been done mostly through non-subject activities such as club activity, group work etc. The survey shows that almost all students think the environmental pollution is serious problem(83% of respondents) and they are especially interested in separate garbage collection(72%) and environmental conservation activity(63.9%) to protect the environment. The concerns about the environmental problems of students(57.7%) has increased after environmental education regardless educational methods or subjects. The students and teachers think that environmental education in school has not been carried out enough and almost all of them(68.4% of students, 97.5% of teachers) agree that the environmental education should be done as an independent subject. In order to more practical and effective environmental education, many opportunities to experience environmental conservation activities should be given during the environmental education course and the environmental education should be separated from other subjects to be taught independently. Moreover, it might be necessary to increase proportion of teachers in environmental disciplines and to develop various teaching methods.

• Parasites, Hosts and Diseases
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• 제22권1호
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• pp.66-71
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• 1984

만손열두조충(Spirometra erinacei)의 성충에 의한 인체감염 2예가 투약후 충체를 수집함으로써 확인되었다. 환자는 강원도 산간지방에 거주하는 24세 및 21세 남자로 복통 및 상복부 통증이 있다고 하였으며 대변에서는 크기 $53-59{\times}37-42{\mu}m$의 조충류 충란이 발견되었다. 치료를 위해 atabrine 500mg과 하제를 투여한 바 3~4시간후 설사편으로부터 조충류 편절이 다수 수집되었다. 수집된 충체는 광선형 여관, 융구(vaginal opening)가 음경낭(cirrus sac)으로부터 분리되어 개구된 점, 저정낭(seminal vesicle)이 음경낭내로 포합된 점, 편절의 이음새부위에도 고환이 분포하는 점, 충란이 길고 한쪽 끝이 뾰족한 경향이 있으며 비대칭형인 점 등으로 Spirometra erinacei(Rudolphi, 1819)로 동정하였다. 환자는 모두 뱀을 날로 먹은 경험이 있으며 따라서 뱀이 감염원이었을 것으로 추측된다.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.164-171
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• 2011

This work focuses on the analysis of bioaerosols in the atmosphere at higher altitudes over Noto Peninsula, Japan. We carried out direct sampling via aircraft, separated cultures, and identified present isolates. Atmospheric bioaerosols at higher altitudes were collected using a Cessna 404 aircraft for an hour at an altitude of 3,500 m over the Noto Peninsula. The aircraft-based direct sampling system was devised to improve upon the system of balloon-based sampling. In order to examine pre-existing microorganism contamination on the surface of the aircraft body, bioaerosol sampling was carried out just before takeoff using the same method as atmospheric sampling. Identification was carried out by a homology search for 16S or 18S rDNA isolate sequences in DNA databases (GenBank). Isolate sampling just before takeoff revealed Stretpomyces sp., Micrococcus sp., and Cladosporium sp. One additional strain, Bacillus sp., was isolated from the sample after bioaerosol collection at high altitude. As the microorganism contamination on the aircraft body before takeoff differed from that while in the air, the presence of additional, higher atmosphere-based microorganisms was confirmed. It was found that Bacillus sp. was floating at an altitude of 3,500 m over Noto Peninsula.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.842-849
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• 2007

The purpose of the present study was to produce live offspring from in vitro fertilized goat embryos. Oocytes were collected from abattoir ovaries and kept in oocyte collection medium. Oocytes were washed 4-5 times with maturation medium containing medium-199 with 5 ${\mu}g/ml$ FSH, 100 ${\mu}g/ml$ LH, 1 ${\mu}g/ml$ estradiol-$17{\beta}$ 50 ${\mu}g/ml$ gentamycin, 10% inactivated estrus goat serum, and 3% BSA (fatty acid free). Oocytes were placed in 100 ${\mu}l$ drops of maturation medium containing granulosa cell monolayer and incubated in a 5% $CO_2$ incubator at $38.5^{\circ}C$ for 27 h. For capacitation of spermatozoa fresh semen was processed and mixed in 3 ml fertilization TALP medium containing 50 ${\mu}g/ml$ heparin and kept in the above incubator for 2 h. The capacitated spermatozoa were coincubated with matured oocytes for fertilization. Cleaved embryos were separated and cultured in embryo development medium with oviductal cells and 494 embryos were produced. Recipient goats were synchronized with two injections of 15 mg $PGF_{{2}{\alpha}}$/goat 10 days apart. Eighty early stage embryos were transferred into the uterotubal junction of 14 surrogate mothers using laparoscopy techniques. One recipient delivered twin kids, whereas another two recipients each.delivered a single kid The parentage of these kids was evaluated using highly polymorphic co-dominant microsatellites markers. From the present study, it was concluded that live goat kids can be produced from in vitro matured and fertilized goat embryos, to the best of our knowledge for the first time in India.

• 유기물자원화
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• 제20권2호
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• pp.76-88
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• 2012

본 연구는 생활계폐기물 중 별도로 분리 배출되어 재활용 선별장으로 반입된 재활용 폐기물에 대한 수집운반에서부터 최종처리까지의 흐름을 분석하였다. 연구는 실제 현장조사를 기본으로 실시하였으며, 조사 결과, 최종 재활용 측면에서는 대체적으로는 적정 처리가 이루어지는 것으로 파악 되었으나, 불필요한 이동 및 중간처리 경로를 통해 처리되는 품목이 상당수 있는 것으로 조사되었다. 지자체에서 선별된 폐자원을 처리 또는 수집운반 하는 업체들은 오로지 경제성 원리에 입각한 폐기물 처리비와 운반비 차익을 주요 수익원으로 하기 때문에 불필요한 중간단계 및 장거리 폐기물 이동현상이 나타나게 된다. 따라서 합리적인 자원순환망 구축을 위한 첫 번째 단계는 폐기물 발생원의 근거리에 있는 재활용업체에서의 우선처리원칙이 필요하며, 필요에 따라 인근 지역의 폐기물을 동시에 처리 할 수 있는 광역처리체계 도입이 타당하다.

• 복식
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• 제57권9호
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• pp.161-176
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• 2007

The objectives of this study are to select and analyze unpublished wedding dresses in the 19th century, to investigate the design characteristics and making techniques of Victorian wedding dresses, and to examine the correlation between the wedding dress style and the fashion style in those days and the influence of the wedding dress style on contemporary's styles. The materials of this study were 9 wedding dresses owned by the Historical Costume and Textile Collection at the University of Connecticut in USA. The dresses were made during the mid and late Victorian Age. As for silhouette, the bustle style was popular in the 1870s and 1880s, and the hourglass style in the 1890s, and different from contemporary dresses there were no big differences in structural pattern and details between ordinary dresses and wedding dresses. In addition, colored wedding dresses were used until the mid and late Victorian Age, but since the wedding ceremony of Queen Victoria in 1840, white wedding dress was widely accepted and settled as the tradition of today's wedding dresses. While the Western dress style had been basically a one piece style, there appeared simple two piece designs in which the upper garment was separated from the lower one, from the mid Victorian Age. This is considered the reflection of those days' pragmatic social trend in dress style. It seems around 1875 when asymmetric design was first introduced in the Western dress style, which had been mostly symmetric. The asymmetric style that pursued atypical beauty, though not common during the late Victorian Age, shows a change in the typical Western dress style. With regard to dress making techniques, sewing machines were distributed widely during the early Victorian Age but they were not used in all parts of dress. Most of details and trimming works were done manually and some parts were attached and detached by hand sewing. In addition, not like today, there were no generalized rules of making such as the form of closings and composition.

• 복식
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• 제57권3호
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• pp.14-22
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• 2007

This study is about the excavated costume representing fundamental "Conservation and Restoration". It's not a report of a specific case, but it is a guideline that contains the costume and textile of museum collection. It is not research based on experiments; however, this paper is basically consists of various reported-documents. Followings are the conclusions of this study 1. These are the factors that we have to know to prevent the causes of fabric's degradation. -Light, -Humidity and temperature, -Microscopic organism, -Insect and rodent animal, -Air pollution, -Ph, -Handling 2. Basic principles of conservation and restoration follows are: -Select the Reversible method, -Represent the easily distinguishable repaired place, -Should be acted by an expert or people with experience, -Before the restoration, accurate and specified records should be completed, -Procedure, treatment method, and materials used should be recorded prior to restoration, -Should be cared minimally, -Be cautious when using the conservation materials, -When caring, make sure nothing is against the principle of aesthetic, historic, and form of preservation 3. The types of restoration are type of straight or curve, type of hole, type of without warp or weft, type of special part damage something like sleeve, collar, type of form that is severely damaged, and type of separated pieces. 4. The method of restoration is sewing, stitching, and the combination of sewing and stitching. 5. The restoration seams are welt seam, plain seam, flat felled seam, french seam etc. And there are kinds of used-sewing, such as, broad stitching, backstitch, half backstitch, basking, hemming, saddle stitching etc.