• Title/Summary/Keyword: seoulensis

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Sequence Analysis and Functional Expression of the Structural and ]Regulatory Genes for Pyruvate Dehydrogenase

  • Hwan Youn;Jangyul Kwak
    • Journal of Microbiology
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    • v.40 no.1
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    • pp.43-50
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    • 2002
  • A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (Elp). Elp had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria S. seoulensis E2p contains two lipoyl domains like those of humans and Streptomyces faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis howed growth retardation and the decrease of Elp, indicating that PdhR regulates the function of PDC by repressing the expression of Elp. A strain of Streptomyces licidans overexpressing S. seoulensis PdhR showed a significant decreasein the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.

Mass-production of Eleutherococcus seoulensis Seedlings Through Somatic Embryogenesis (체세포배 형성을 통한 서울오갈피(Eleutherococcus seoulensis) 묘목의 대량생산)

  • Lee, Su-Gwang;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.719-725
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    • 2009
  • This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

Pharmacognostical Studies on the "Se Sin" (세신의 생약학적 연구)

  • 박정희
    • Korean Journal of Plant Resources
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    • v.9 no.2
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    • pp.183-188
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    • 1996
  • Chinese crude drug 'Se Sin'(細辛)has been used to cure headache, cough and expectorant. To the botanical origin of 'Se Sin', Zhong-yao-zhi(中葯志) reported Asarum heterotropoides var. mandshuricum. A. sieboldii and A. sieboldii var. seoulensis, and Zhong-yao-da-ci-dian(中葯大辭典) reported Asarum heterotropoides var. mandshuricum and A. sieboldii of Aristolochiaceae. In Korea, this crude drug has been used as a Korean folk remedy for tothache and aromatic. The botanical origin of 'Se Sin' is considered to be Asarum species of Aristolochoaceae. But there has no pharmacognostical confirmation on it. To clarify the botanical origin of 'Se Sin', we studied on the anatomical characteristics of Asarum species growing wildly in Korea i.e. A. maculatum, A. sieboldii, A. sieboldii var. seoulensis, and of 'Se Sin' from Korea on Korean market. Through our studies, the botanical origin of 'Se Sin' from Korea was proved to be whole plant of Asarum sieboldii and A. sieboldii var. seoulensis.

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Transmission electron microscopic findings of the tribocytic organ of Fibricola seoulensis (Fi'bricola seoulenis 조직융해구의 투사전자현미경 소견)

  • 송호복
    • Parasites, Hosts and Diseases
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    • v.31 no.4
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    • pp.315-320
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    • 1993
  • We observed ultra-structure of the tribocytic organ of Firicola seoulensis with transmission electron microscope. Microvilli are observed on the surface of the tribocytic organ. Below the muscle layer, we can find three types of cells. Type I cell has electron lucent cytoplasm with a few granules, while type II cell shows electron dense cytoplasm and the particulate granules. Type III cell's electron dense cytoplasm possesses many granules. Of the above three cells, Type I and II cells are believed as tegumental cells. Type III cells are thought as glandular cells specific to the tribocytic organ. This finding on also explain the secretory function of the tribocytic organ of f seoulensis.

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Transmission electron microscopic ultrastructures of the male germinal cells of Fibricola seoulensis (Fibricola seoulensis 웅성생식세포의 투과전자현미경적 미세구조)

  • 손원목;이순형
    • Parasites, Hosts and Diseases
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    • v.31 no.3
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    • pp.183-192
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    • 1993
  • A transmission electron microscopic study was performed to observe the ultrastructures of the male germinal cells and spermatozoa of Fibricola seoulensis. Spermatogonia were found in the periphery of the testis and characterized by large nuclei and comparatively little cytoplasms. Spermatocytes contained an oval to spherical nucleus. Their nuclear volume was little larger in comparative to that of cytoplasm, and the chromatin was comparatively little. The early spermatids were characterized by a great amount of cytoplasm, and numerous mitochondria encircled the nucleus. In a more advanced spermatids the electron-dense strands of chromatin appeared in the nucleus, and a pair of rootlet of the axoneme and a microtubule-organizing center (MTOC) were observed near the nucleus. The sectioned spermatozoa were found in the testis and the seminal vesicle. Their cross sectional views were divided into 6 types when they were distinguished on the basis of the morphology and components. The spermatozoa of F. seoulensis showed two flagella of 9+1 type axoneme.

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Immunological Relationship of Crystal Proteins of Six Bacillus thuringiensis Serovarieties; B. thuringiensis serovar. coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41) andyosoo (H18a18c)

  • Lee, Kwang-Yong;Kang, Eun-Young;Kwon, Hyuk-Han;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.415-416
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    • 2004
  • Crystals of six new Bacillus thuringiensis serovarieties [coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41), and yosoo (HI8a18c)] with different H-antigens, which are toxic to Bombyx mori and/or mosquito larvae, were serologically quite distinct from each other.

Nickel Binding Protein (NBPI) and Its Gene from Streptomyces seoulensis

  • Kim, In-Kwon;Sa-Ouk kang
    • Proceedings of the Korean Biophysical Society Conference
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    • 2001.06a
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    • pp.52-52
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    • 2001
  • Nickel-binding protein (NBP1) was purified from the crude extract of Streptomyces seoulensis using Ni$^{2+}$-charged metal chelate affinity chromatography. The molecular mass of NBPI determined on SDS-PAGE was 38kDa. An approximately 3 kb DNA fragment containing the structural gene for NBP1 was cloned from lEMBL3 genomic library of S. seoulensis using a DNA fragment PCR-amplified with the primers designed from N-terminal and internal amino acid sequences of NBP1.(omitted)

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Ten Human Cases of Fibricola seoulensis Infection and Mexed One with Stellantchasmus and Metagonimus (Fibyicola seoulensis감염 10례 및 Htelluntchasmus falcatus감염 1례)

  • Hong, Seong-Tae;Chae, Jong-Il;Lee, Sun-Hyeong
    • Parasites, Hosts and Diseases
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    • v.24 no.1
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    • pp.95-97
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    • 1986
  • 서울에 소재한 육군장병의 검변에서 Fibricola의 충란 양성자 10명을 관찰하여 프라지관텔로 치료하고, 이 증 한례에서 Fibricola seoulensis의 성충 59마리, 요꼬가와홉충 68마리 및 Stellantchasmus fnlcatus 2마리를 관찰하였다

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