• Plant Resources
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• v.2 no.1
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• pp.22-25
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• 1999

Molecular markers are useful to confirm the hybridity of F1 plant derived from cross of two homozygous parents with similar morphological traits. RAPD markers were used to test F1 hybrid plant obtained from cross of two homozygous soybean (Glycine max) parents. Fl plant for cross I was made from the mating of Hobbit87 (female) and L63-1889 (male) and Fl plant for cross II was obtained from the mating of H1053 (female) and L63-1889 (male). Selfing plant per each cross was also obtained. Among 20 Operon primers used, OPA04 and OPA09 show polymorphism between cross I and II parent. Band in size 1Kb of OPA04 and 2.1Kb of OPA09 primer was polymorphic band. This fragment identified Fl hybrid plant and selfing plant in cross I and II. Female parent Hobbit87 in cross I and H1053 in cross II has no this fragment (recessive allele). However, male parent L63-1889 and Fl hybrid plant in cross I and II has this size of polymorphic band (dominant allele). This indicated that Fl hybrid and selfing plants were detected by RAPD marker before phenotypic marker would be used to identify Fl hybridity. Amplification products of selfing plant for cross I and II were completely same to the those of female parent. When mature, flower color of Fl hybrid plant in cross I and II was purple and flower color of selfing plant in cross I and II was white. Purple flower is dominant trait. Fl hybridity was successfully detected at very early growth stage using RAPD marker. Therefore, RAPD marker can be used broadly to confirm Fl hybridity in many crops.

• Journal of Plant Biology
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• v.38 no.1
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• pp.39-45
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• 1995

Glycine soja Sieb. et Zucc., a predominantly selfing annual, has been served as a reservoir of germplasm for soybean, G. max (L.) Merr., cultivar improvement. This study describes the levels and distribution of genetic variation within and among 22 Korean populations of G. soja using starch gel electrophoresis. The species maintains very similar levels of genetic variability within populations observed in most other annuals. At the population level, the mean percent of polymorphic loci (P) was 32.6%, mean number of allele per locus (A) was 1.32, and mean expected heterozygosity (He) was 0.112. In addition, total genetic diversity (HT) calculated only for polymorphic loci was 0.347. However, significant differences in allele frequencies among populations were found for all loci (P<0.001 in each case) and, on average, about 70% of the total variation in the species is common to all populations. Indirects estimate of the number of migrants per generation (Nm=0.58, calculated from mean GST) indicates that gene flow is low among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygote deficiency in most populations and at all loci. This indicates that most populations sampled may have been substructed largely due to inbreeding (predominantly selfing) and restricted gene flow, coupled with founder effect and genetic drift. Considering a high genetic divergence among populations, it is recommended that several Korean populations of the species should be preserved, especially such as populations in the eastern and southeastern Korean peninsula with high variation.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.319-323
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• 2009

Strawberry is an out-crossed crop that is relatively sensitive to inbreeding, and as a result, most of breeding programmes in strawberry have been based on selection breeding where elite parents are selected from intercrossing. Also, pedigree breeding has been successfully employed in a number of instances to concentrate genes of interest. Therefore, in order to obtain inbred line having the genotype of excellent combining ability by selfing and incrossing to improve breeding efficiency of strawberry, elite varieties were self-pollinated. From the selfing line of "Benihoppe" cultivar, we selected "Wongyo 3111" having excellent horticultural traits such as plant vigor, sugar content and fruit hardness among these progenies. "Wongyo 3111" shows erect plant type, vigorous growth habit, early flower bud differentiation with 16~17 flowers per cluster. Fruits of "Wongyo 3111" are conical type having a bright red skin color, and 17.2 g in an average weight. Also that have a relatively more high sugar/acid ratio and firmness than check cultivars as sugar content of 11.3oBx, acidity of 0.50% and firmness of 21.7 g/$mm^2$. But although yield of "Wongyo 3111" is not significantly different from check cultivars, its marketable yield is remarkably lower than that of check cultivars because of poor fruit-setting under low temperature. Disease and pest reactions of "Wongyo 3111" are more sensitive to powery mildew, anthracnose and spotted spider mites than check cultivars. Therefore, "Wongyo 3111" is suitable for intermediate parent line better than as a cultivation cultivar.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.41-42
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• 1998

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.39-40
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• 1998

• Asian Journal of Turfgrass Science
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• v.9 no.2
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• pp.137-157
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• 1995

Some indices of species of the Arundinella hirta communities in Cheolwon area were studied and the following results were obtained. The vegetational date was analyzed from six grass stands selected with the distance away from the DMZ in Cheolwon area. Species diversity was higher in the stand away from the DMZ than in that neat to it. All six stands are not considered to be free of disturbance. Species composition of the stands might be related to the degree of disturbance. It can be said that the destruction of the vegetation by periodical fire or cutting simplifies the species composition. In the rear district the human inter ference is supposed to be more frequent. But it is supposed to be slight in near the DMZ and that the degree of disturbance is not so destructive. This could be the explanation of high diversity in these stands.

• Proceedings of the KSR Conference
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• 2004.06a
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• pp.309-314
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• 2004

This study focused on the introduction of damage repair for polymeric composite carbody. called selfing tech-healinique. using microcapsules loaded with the healing agent The manufacturing process for microcapsules with the healing agent was introduced and tile characteristics of microcapsules manufactured by varying with various manufacturing process variables were evaluated. The DCPD was used for the healing agent and microcapsules were made of urea-formaldehyde resin. The magnitude and the size distribution of microcapsules were measured by a particle size analyzer using laser diffraction technique. Thermal stability was investigated by using a TGA under continuous and isothermal heating conditions for the healing agent. microcapsules without the healing agent. microcapsules with the healing agent.

• Korean Journal of Microbiology
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• v.13 no.3
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• pp.123-137
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• 1975

Sexual reproduction of paramecia have been accomplished through conjugation between individuals which have opposite mating type substances on their cilia when they were starved. Using selfing clone in which mating takes place, I examined whether a mating type change in indicidual cells required new protein and new mRNA synthesis or not and also shether there is a precursor relationship between both of the complementary mating type substances in their synthetic pathway. I found that 1. Mating type change needs new protein(s) and new mRNA synthesis. 2. Mating type substances are synthesized sequentially from mating type XIII to XIV 3. There might be a common precursor pool from which the mating type XIII substnace is synthesized and then complementary mating type XIV is fromed by addition of small group to the mating type XIII substance.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.131-134
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• 1998

$\textrm{T}_{5}$ progeny of one transgenic tomato line (To9) carrying antisense polygalacturonase (PG) cDNA was generated by selfing. Five $\textrm{T}_{5}$ plants were used to analyse in detail. The PG antisense gene was stably inherited through fifth generations. In all five $\textrm{T}_{5}$ plants, expression of the antisense transcripts were detected. In consequence, it led to a reduction of the PG enzyme activity in ripe fruit to between 37% and 65% that of normal. In two plants the expression of endogenous PG gene was inhibited in ripe fruit.

• Korean Journal of Ecology and Environment
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• v.50 no.1
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• pp.116-125
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• 2017

Alpine plants with a scarcity of pollinators in harsh environments have been believed to undergo selfing for reproductive assurance; however, contradictory evidence is also available. Snowmelt regimes in alpine areas function to change life history characteristics of alpine plants such as flowering time and duration; yet the effects of snowmelt regimes have never been tested in alpine plants in Korea. This study was conducted to investigate the dichogamy, mating systems, and flowering characteristics of Megaleranthis saniculifolia populations [early and late snowmelt plots (ESP and LSP, respectively)] in a subalpine area of Sobaeksan Mountain in Korea. M. saniculifolia exhibited incomplete protogyny in that despite early maturation of pistils, maturation times of pistils and stamens within flowers were partly overlapped. Control and hand-outcrossing treatments produced significantly higher number of follicles and seeds per flower than autonomous and hand-selfing treatments. Based on the aggregate fruit set, the auto-fertility index (AI) and self-compatibility index (SI) were 0.33 and 0.50, respectively. Snowmelt occurred 10 days earlier in ESP than in LSP, thereby ESP and LSP showed distinct differences with regard to flower longevity and season, but showing no difference in peak flowering dates. We concluded that M. saniculifolia is an incomplete protogynous and largely outcrossing plant requiring pollinator service. Temporal variation in snowmelt time and subsequent changes in flowering characteristics under climate change may further threaten the population persistence of M. saniculifolia which has already been designated as endangered species in Korea.