A flue-cured tobacco variety (Nicotiana tabacum cv. Wisconsin) was used for Plant transformation with the complementary DNA (cDNA) of potato virus Y-necrosis strain (PVY-VN) replicase gone (Nb) which was synthesized through reverse-transcription Primed with oligo(dT) and Polymerization using RNase H-digested template. The cDNA was cloned into Plant expression vector Plasmid (PMBP2), and introduced into tobacco plants by co-culturing tobacco leaf disks with Agrobacterium tumefaciens LBA4404 containing the plasmid before Plant regeneration. Eight Plants, in which the inserted cDNA fragment was detected by Polymerase chain reaction (PCR), out of 70 putative transformants inserted with sense-oriented Mb cDNA showed no symptom at 3 weeks after inoculation, while the other 62 plants, and all plants with vector gone only and antisense-oriented NIb cDNA had susceptible vein-necrosis symptoms. However, only 2 of the 8 resistant plants were highly resistant, which remained symptomless up to 10 weeks after inoculation. Among the first progenies (T1) from self-fertilized seeds of the two resistant transgenic plants, less than 10 % of 71 plants appeared highly resistant (with no symptom), 70% moderately resistant (with mild symptoms on 1 - 2 leaves), and about 20% susceptible (with susceptible symptoms on 3 or more leaves) at 3 weeks after inoculation. These results suggest that the PVY resistance was inherited in the 71 generation. Key words : potato virus Y. viral replicase gene, transgenic tobacco Plants, resistance.
The purpose of this study was to understand the new generation's culture and to analyze if the characteristics of their lifestyle is significant for the study of new generation's fashion. The new generation's characteristics of lifestyle can be described as a strong pride of their own self and the life which expresses that pride. To analyze this characteristics connecting it with fashion, it is as follows: -It is the egocentric sensitivity culture. In their fashion, there appears a tendency that sense and individuality are counted much rather than fashion. -Diverse fashion information is gained through the mass media and communication. The sense for fashion is liable to change according to the circumstances and, simulated world is made. -The sense of denial is not shown much to the western lifestyle and way of thinking owning to the international open culture. Their consciousness and favorite of fashion are beyond the border. The new generation showed the unprecedent way of expression, denying the established form, and this is defined as Borderless fashion. The Borderless fashion leads a whole trend of new generation's fashion in the late part of ninties. Two typical trends appear as Genderless look for the individualic group and Romanticism look laying stress on womanliness.
The Journal of Asian Finance, Economics and Business
/
v.7
no.10
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pp.855-863
/
2020
The purpose of this study is to investigate the impacts of two different kinds of interactivity, such as user-to-user and user-to-media interaction, on the relationship between SNS motivation and online word of mouth (WOM). An online survey was conducted with SNS users in Korea. Using the convenience-sampling method, 300 surveys were collected and 295 were used in the actual analysis after excluding data with careless responses or missing values. Hypotheses were tested using Structure Equation Model (SEM) and path analysis by using AMOS22. The results indicate that four different SNS motivations (self-expression, relational, fun, and browsing motivation) have a partially significant positive effect on perceived user-to-user and user-to-media interaction in SNS. Although both user-to-user interactivity and user-to-media interactivity were found to have a significant effect on online word of mouth, by comparing the standardized regression coefficients in these relationships, it was found that user-to-user interactivity has a greater effect on online WOM than user-to-media interactivity. These results show that the motivated SNS users want to express their desire to communicate with other users in contrast than their desire to learn media functions when motivated SNS users reveal their personalities, knowledge, and abilities. Theoretical and managerial implications are discussed.
Journal of the Korean Institute of Intelligent Systems
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v.13
no.6
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pp.743-748
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2003
In this research, we propose a mechanism to develop an inference engine and expert systems based on relational database (RDB) and SQL (structured query language). Generally, former researchers had tried to develop an expert systems based on text-oriented knowledge base and backward/forward (chaining) inference engine. In these researches, however, the speed of inference was remained as a tackling point in the development of agile expert systems. Especially, the forward inference needs more times than backward inference. In addition, the size of knowledge base, complicate knowledge expression method, expansibility of knowledge base, and hierarchies among rules are the critical limitations to develop an expert system. To overcome the limitations in speed of inference and expansibility of knowledge base, we proposed a relational database-oriented knowledge base and forward inference engine. Therefore, our proposed mechanism could manipulate the huge size of knowledge base efficiently. and inference with the large scaled knowledge base in a short time. To this purpose, we designed and developed an SQL-based forward inference engine using relational database. In the implementation process, we also developed a prototype expert system and presented a real-world validation data set collected from medical diagnosis field.
The Main objective of this study was to investigate the relationship between shopping orientations and Knit wear Buying Behavior of college female students. A questionnaire was developed to measure knit wear purchasing motives, fashion information sources of knit wear, evaluation criteria of knit wear product, and general clothing buying behavior. The questionnaire was administered to 505 college female students in Chonbuk and Chonnam. The data was analyzed using percentage, frequency, mean, factor analysis, Cluster Analysis and ANOVA, Duncan Multiple Range test. The results of the study were as follows: 1. The college female students were classified into fifth subdivisions by the cluster analysis; convenient shopping group, recreational shopping group, self-confident shopping group, those of Fashion-pursuit group, economic shopping group. 2. The knit wear purchasing motives of consumers were significantly different according to shopping orientation subdivision in social, personal, rational. 3. In the case of fashion information sources of knit wear, significant differences were found according to shopping orientation subdivision in mass media information, information by marketer, mail order advertisements, information by consumer. 4. The evaluation criteria of knit wear product of consumers were significantly different depending on shopping orientation subdivision in esthetic, practicality, individual expression, external criterion. 5. In the case of dissatisfactory factors for wearing knit wear product, significant differences were found according to shopping orientation subdivision. 6. In the case of asking factors to the manufactures of knit wear product, significant differences were found according to shopping orientation subdivision.
Journal of the Korea Fashion and Costume Design Association
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v.17
no.4
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pp.131-144
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2015
The object of this study was to find out the influence of female consumers' cosmetics purchase experience on image-making efficacy and cosmetics pursuit benefits. Total 405 surveys of women working at the industrial complex in Daegu-Kyoungbuk area were used for data analysis. Frequency, factor analysis, reliability analysis, regression analysis, and ${\chi}^2$-test were used for data analysis. Our findings are as follows. Cosmetics purchase experience of female consumers had factors as recognition experience, relationship experience, emotional experience, visit experience, and sensual experience. The factors of image-making efficacy were self-confidence in displaying, ability of facial expression and display ability while those of cosmetics purchase experience turned out to be trend/brand pursuit, appearance improvement pursuit, functionality pursuit and economic feasibility pursuit. Cosmetics purchase experience was found to have significant effects on the factors of image-making efficacy, and cosmetics purchase experience on factors of cosmetics pursuit benefits. The differences by ages variable was examined with relation to the level of make-up and how they chose products when buying.
Journal of Korean Academy of Nursing Administration
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v.20
no.3
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pp.332-341
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2014
Purpose: The purpose of this study was to investigate the relationship among turnover intention, emotional labor, and communication competence in nurses. Methods: The participants for this study were 297 nurses from three general hospitals in two local cites in Korea. Data were collected by self-administered questionnaires from August 26 to September 10, 2013 and analyzed using descriptive statistics, One-way ANOVA, t-test, Pearson Correlation, Stepwise Multiple Regression with the SPSS/WIN 18.0 program. Results: The average scores for turnover intention, emotional labor, and communication competence respectively, were 3.45, 3.08, and 3.44 out of 5. The novices recognized that their emotional labor and turnover intention were significantly higher, and their communication competence was lower than other nurses. Nurses' turnover intention had a positive relationship with their emotional labor, but no relationship with communication competence. Job satisfaction, frequency of emotional expression, and emotional dissonance had an effect on nurses' turnover intention. Conclusion: The results show that emotional labor and job satisfaction are very important factors affecting nurses' turnover intention. So, nurse managers should try to minimize nurses' emotional labor and maximize their job satisfaction by developing various human relationship educational and support programs and using them.
Purpose: The purpose of this study was to define and clarify the concept of political competence for nurses. Methods: A hybrid model method was used to investigate the dimensions, attributes, and definitions of the concept. In the theoretical stage of the study, literature on nursing, politics, and other discipline were reviewed. In the fieldwork stage, individual in-depth interviews and focus groups interviews were conducted with politically seasoned experts or activists who had an understanding of the concept of political competence for extensive descriptions in nursing and field of health care. Results: The concept of political competence was represented in four dimensions as political knowledge, political efficacy, political interaction, and political activity. In the political knowledge dimension, there were three attributes, namely, political knowledge, political information and systematic analysis ability. The political efficacy dimension had three attributes of internal political efficacy, external political efficacy, and self-pride of nursing profession. The political interaction dimension had three attributes of organizations and community service, networking, and persuasive power. The political activity dimension had six attributes of political leadership, political expression, assertive behavior, political advocacy, political participation, and policy intervention. Conclusion: This concept development might provide a basic understanding of developing a measurement tool and for constructing a theory promoting nurses' political competence.
The current study examined the ability to predict Gottman's four dysfunctional communication behaviors during marital arguments based on the level of surface and core problems in marriage. Core problems were composed of three factors: caring, power, and commitment problems. A self-report questionnaire was administered to 182 married women residing in Busan. The results of multiple regression analyses revealed that a woman's degree of criticism was predicted by surface and caring problems when controlling for the other variables. The degree of contempt was predicted by a commitment problem as well as surface and caring problems. Defensiveness was determined only by a commitment problem, while stonewalling was determined only by a power problem. Overall, these results showed the importance of core problems in understanding the causes of the four dysfunctional communication behaviors - especially contempt - and suggested that additional attention should be given to a commitment problem as a contributor to the expression of contempt and defensiveness.
The purpose of this study was to investigate the emotional ability between abused children and general children by comparing their emotional intelligence and emotional control. Participants were 17 abused children who had been separated from their abusers and 17 general children, all elementary school students. The answers to the questionnaire items on emotional intelligence and situations of emotional motivation were analyzed by Mann-Whitney U as a study tool. The results of this study were as follows: First, the difference of emotional intelligence between abused children and general children was statistically significant. Abused children received lower scores than general children when it came to their emotional recognition, emotional expression, empathy, and emotional regulation as a subordinate scope of emotional intelligence. Second, the difference of emotional regulation strategy between abused children and general children was statistically significant. Abused children presented negative responses and less frequently used positive strategy, inhibitory avoidance strategy and alternative strategy than general children. Third, the difference of emotional regulation motivation between abused children and general children was statistically significant. Abused children presented less prosocial motivation, motivation of self-preservation and normative motivation than general children.
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