• 제목/요약/키워드: self-assembly technique

검색결과 49건 처리시간 0.027초

Surface Plasmon Resonance Immunosensor for Detection of Legionella pneumophila

  • Oh, Byung-Keun;Lee, Woochang;Bae, Young-Min;Lee, Won-Hong;Park, Jeong-Woo
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권2호
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    • pp.112-116
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    • 2003
  • An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by Self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amino (-NH$_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au Substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The Surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 10$^2$CFU per mL.

이송조립기술로 제조된 나노 박막의 기계적인 특성 평가에 관한 연구 (A study on mechanical characterization of nano-thick films fabricated by transfer assembly technique)

  • 최현주;김재현;이상주;이학주
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2008년도 추계학술대회A
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    • pp.30-34
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    • 2008
  • The transfer assembly (or transfer printing) technique is a promising method for fabricating multi-scale structures on various substrates including semiconductors and polymers, and has been applied to fabrication of flexible devices with superior performance to conventional organic flexible devices. The mechanical behaviors of the structures fabricated by the transfer assembly is a very important information for design and reliability evaluation purpose, but the measurement of the behaviors is difficult since their critical-dimensions are very tiny. In this study, Au films with nano-scale thickness were fabricated on a silicon substrate and their mechanical properties were measured using micro-tensile test. The Au films on the silicon substrate were then transferred to a PDMS substrate using the transfer assembly technique. Self-assembled monolayer (SAM) with a thiol group was used to enhance the transfer of Au films, and the mechanical behaviors were characterized using wrinkle-based test. The test results from micro-tensile and wrinkle-based test are compared to each other, and their implication to the transfer assembly technique is discussed.

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Three-Dimensional Self-Assembled Micro-Array Using Magnetic Force Interaction

  • Park, Yong-Sung;Kwon, Young-Soo;Eiichi Tamiya;Park, Dae-Hee
    • KIEE International Transactions on Electrophysics and Applications
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    • 제3C권5호
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    • pp.182-188
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    • 2003
  • We have demonstrated a fluidic technique for self-assembly of microfabricated parts onto substrate using patterned shapes of magnetic force self-assembled monolayers (SAMs). The metal particles and the array were fabricated using the micromachining technique. The metal particles were in a multilayer structure (Au, Ti, and Ni). Sidewalls of patterned Ni dots on the array were covered by thick negative photoresist (SU-8), and the array was magnetized. The array and the particles were mixed in buffer solution, and were arranged by magnetic force interaction. Binding direction of the metal particle onto Ni dots was controlled by multilayer structure and direction of magnetization. A quarter of total Ni dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost even with the Au surface on top. The particles were successfully arranged on the array.

다층박막법을 이용한 표면 젖음성 제어 기술 동향 (Technology Trend of surface Wettability Control Using Layer-by-Layer Assembly Technique)

  • 성충현
    • 접착 및 계면
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    • 제18권4호
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    • pp.171-178
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    • 2017
  • 최근 들어, 다층박막법(Layer-by-Layer(LbL) assembly)을 이용한 표면 젖음성 제어 기술이 큰 관심을 받고 있다. 다층박막법은 고분자, 계면활성제, 나노 입자 등과 같은 다양한 재료를 이용하여 수직 구조와 표면 특성을 나노 및 마이크로 스케일로 제어할 수 있는 다기능적이며 친환경적인 제조방법이다. 본 논문에서는 다층박막법을 이용하여 표면 특성을 제어하는 기술의 최근 동향을 살펴보고자 한다. 특히, 초발수, 초친수, 초발유/초친수 LbL 표면의 제조와 응용에 대한 기술 동향과 연구 결과를 기술한다. 또한, omniphobic, 자가-치유, 지능형 및 외부 반응형 표면 등 최근 각광을 받고 있는 분야의 기본적인 원리와 제조 방법 등에 대해 소개하고자 한다.

소수성 상호작용을 이용한 고집적 DNA칩 마이크로어레이의 개발 (Development of High-Intergrated DNA Chip Microarrays by Using Hydrophobic Interaction)

  • 김도균;최용성;권영수
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 하계학술대회 논문집
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    • pp.757-760
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    • 2001
  • We have used the random fluidic self-assembly (RFSA) technique based on the chip pattern of hydrophobic self-assembly layers to assemble microfabricated particles onto the chip pattern. Immobilization of DNA, fabrication of the particles and the chip pattern, arrangement of the particles on the chip pattern, and recognition of each using DNA fluorescence measurement were carried out. Establishing the walls, the arrangement stability of the particles was improved. Each DNA is able to distinguish by using the lithography process on the particles. Advantages of this method are process simplicity, wide applicability and stability. It is thought that this method can be applicable as a new fabrication technology to develop a minute integration type biosensor microarray.

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Two-dimensional Chiral Honeycomb Structures of Unnatural Amino Acids on Au(111)

  • Yang, Sena;Jeon, Aram;Lee, Hee-Seung;Kim, Sehun
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.191.1-191.1
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    • 2014
  • Crystallization has become the most popular technique for the separation of enantiomers since the Pasteur's discovery. To investigate mechanism of crystallization of chiral molecules, it is necessary to study self-assembled structures on two-dimensional surface. Here, we have studied two-dimensional self-assembled structures of an unnatural amino acid, (S)-${\beta}$-methyl naphthalen-1-${\gamma}$-aminobutyric acid (${\gamma}^2$-1-naphthylalanine) on Au(111) surface at 150 K using scanning tunneling microscopy (STM). At initial stage, we found two chiral honeycomb structures which are counter-clockwise and clockwise configurations in one domain. The molecules are arranged around molecular vacancies, dark hole. By further increasing the amounts of adsorbed ${\gamma}^2$-1-naphthylalanine, a well-ordered square packed structure was observed. In addition, we found the other structure that molecules were trapped in the pore of the hexagonal molecular assembly.

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Field Emission from Single-Walled Carbon Nanotubes Aligned on a Gold Plate using Self-Assembly Monolayer

  • Lee, Ok-Joo;Jeong, Soo-Hwan;Lee, Kun-Hong
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2002년도 International Meeting on Information Display
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    • pp.305-308
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    • 2002
  • Field emission from single-walled carbon nanotubes (SWNTs) aligned on a patterned gold surface is reported. The SWNTs emitters were prepared at room temperature by a self-assembly monolayer technique. SWNTs were cut into sub-micron length by sonication in an acidic solution. Cut SWNTs were attached on the gold surface by the reaction between the thiol groups and the gold surface. The field emission measurement showed that the turn-on field was 4.8 $V/{\mu}m$ at the emission current density of 10 ${\mu}A/cm^2$. The current density was 0.5 $mA/cm^2$ at 6.6 $V/{\mu}m$. This approach provides a novel route for fabricating CNT-based field emission displays.

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Direct Coloration using Self-assembly Fabrication Method on PET Fibers - Surface diazo coupling reaction -

  • Kim, Byung-Soon;Son, Young-A
    • 한국염색가공학회지
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    • 제19권5호
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    • pp.37-40
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    • 2007
  • The electrostatic layer-by-layer technique provides a convenient way to control the construction of ultrathin films at nano-scale ranges and can be easily obtained. It can be also applicable to fiber substrate with dye compounds. We have fabricated multilayer dye films using diazonium resin and three couplers, which are prepared by self-assembly approach. This method is based on layer-by-layer deposition using electrostatic attraction between oppositely charged ions. Beside, the diazo coupling reaction proceeded to form azo dye layer on the PET fibers the same time. The corresponding results of the multilayer films have been discussed on the level of color strength (K/S).

유기 단분자막을 이용한 마그네슘 합금 판재 (AZ31)의 내식성 표면처리 기술 연구 (Anti-corrosive surface treatment of Mg alloy steel using organic self assembly monolayer technique)

  • 박종원;이경황;박영희
    • 한국표면공학회:학술대회논문집
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    • 한국표면공학회 2009년도 춘계학술대회 논문집
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    • pp.113-114
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    • 2009
  • 기존의 습식 및 건식 표면처리 공정의 단점을 극복하고, 마그네슘 합금의 가장 큰 문제점인 내식성개선을 위해 열화학기상증착(thermal Chemical Vapor Deposition)법을 이용하여 자기조직화 유기 단분자막(Self-Assembled organic Monolayer, SAM)을 제작하여 마그네슘 합금(AZ31)의 내식성을 검토하였다.

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Fabrication and Manipulation of Gold 1D Chain Assemblies Using Magnetically Controllable Gold Nanoparticles

  • Kim, Lily Nari;Kim, Eun-Geun;Kim, Junhoi;Choi, Sung-Eun;Park, Wook;Kwon, Sunghoon
    • Bulletin of the Korean Chemical Society
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    • 제33권11호
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    • pp.3735-3739
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    • 2012
  • We have developed magnetically controllable gold nanoparticles by synthesizing superparamagnetic $Fe_3O_4$ core/gold shell nanoparticles. The core/shell particles have the capability of forming gold 1D chains in the presence of an external magnetic field. Here we demonstrate dynamic and reversible self-assembly of the gold 1D chain structures in an aqueous solution without any templates or physical or chemical attachment. The spatial configuration of gold chains can be arbitrarily manipulated by controlling the direction of a magnetic field. This technique can provide arbitrary manipulation of gold 1D chains for fabrication purpose. To demonstrate this capability, we present a technique for immobilization of the gold particle chains on a glass substrate.