• BMB Reports
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• v.57 no.1
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• pp.40-49
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• 2024

Prokaryotes encode clustered regularly interspaced short palindromic repeat (CRISPR) arrays and CRISPR-associated (Cas) genes as an adaptive immune machinery. CRISPR-Cas systems effectively protect hosts from the invasion of foreign enemies, such as bacteriophages and plasmids. During a process called 'adaptation', non-self-nucleic acid fragments are acquired as spacers between repeats in the host CRISPR array, to establish immunological memory. The highly conserved Cas1-Cas2 complexes function as molecular recorders to integrate spacers in a time course manner, which can subsequently be expressed as crRNAs complexed with Cas effector proteins for the RNA-guided interference pathways. In some of the RNA-targeting type III systems, Cas1 proteins are fused with reverse transcriptase (RT), indicating that RT-Cas1-Cas2 complexes can acquire RNA transcripts for spacer acquisition. In this review, we summarize current studies that focus on the molecular structure and function of the RT-fused Cas1-Cas2 integrase, and its potential applications as a directional RNA-recording tool in cells. Furthermore, we highlight outstanding questions for RT-Cas1-Cas2 studies and future directions for RNA-recording CRISPR technologies.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.68-77
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• 2017

This study investigated the relationships among major satisfaction, nursing professionalism, and college life adaptation of the nursing students in order to present basic data to assist in adaption of college life. The study subjects were 553 nursing students from two universities in K and P cities by self-report questionnaire survey. Data were collected between November 7 and November 25, 2016. The data were analyzed through frequency analysis, descriptive statistics, t-test, ANOVA, Scheffe test, Pearson correlation coefficient, and hierarchical regression using SPSS 21.0 program. As a result, major satisfaction, nursing professionalism, and college life adaptation averaged 3.86(out of 5), 3.97(5), and 3.34(5) respectively. There was a significant positive correlation between major satisfaction and nursing professionalism, college life adaptation on nursing students. In addition, nursing professionalism had moderating(F=8.460, p=.004) and partial mediating (${\beta}=.21$, p<.001) effects between major satisfaction and college life adaptation among nursing students. In conclusion, nursing professionalism is a very important factor in the effects of major satisfaction of nursing students on college life adaptation. Therefore, in order to promote college life adaptation of nursing students, and increase their major satisfaction, it is necessary to develop and apply an educational program that can establish the right nursing professionalism.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.113-126
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• 2016

The main purpose of this study is to provide the necessary information for educational intervention by discovering the components and factors related to the adaptation experience of male students in the department of nursing. The participants were seven male nursing students. Data were collected from in-depth interviews, and the results were analyzed by the Giorgi's phenomenological analysis method. The main components of adaptation experience of participants were summarized into four categories: 'hopes and expectations for the future careers,' 'conflicts and wandering under realistic issues,' 'compromise and emotion management,' and 'efforts for self-growth.' Causative, accelerative, and recursive characteristics were shown among these components. The adaptation process was structured in the temporal context. The results suggest that for amicable adaptation, internal controls, such as 'improving willpower,' 'inspiring solid career awareness,' and 'selecting adaptation strategy' were all critical. Moreover, the support of families, relatives, college seniors, and department professors were revealed as the factors that help in the adaptation experiences. Based on the results, further studies are required to identify the dropout rates by maladaptation of male nursing students, and analyze possible factors that contribute to dropout and maladaptation. In addition, it is necessary to construct various coping methods and practical support systems, as well as to develop appropriate educational programs.

• Journal of Digital Convergence
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• v.17 no.11
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• pp.387-394
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• 2019

The purpose of this study was to identify the moderated effects of emotional intelligence on the relationship between transition shock and adaptation on clinical practice among nursing students. Subjects were 185 associate nursing students associate nursing students(3rd). The data were collected using self -report questionnaire from May 1 to June 14, 2019. Data were analyzed by descriptive statistics, t-test, ANOVA, Pearson correlation and hierarchical multiple regression with SPSS/WIN 23.0. Mean point of transition shock(4 point scale) was 2.32. Mean point of emotional intelligence(5 point scale) was 3.61, and adaptation on clinical practice(5 point scale) was 3.40. Transition shock were significantly negative correlation with emotional intelligence(r=-.26. p <.001) and adaptation on clinical practice(r=-.43. p <.001) in nursing students. Emotional intelligence were significantly positive correlation with adaptation on clinical practice(r=.36. p <.001). Emotional intelligence has been shown to modulate the relationship between transition shock shock and adaptation on clinical practice(${\Delta}R^2=0.21$, p <.001). Therefore, to decrease the transition shock and to increase the adaptation on clinical practice of nursing students, It is necessary to develop the program for increase emotional intelligence of nursing students.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.2
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• pp.159-175
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• 2018

The purpose of this study was to identify the factors that could overcome the crisis and adversity of the nursing care provider through understanding the effect of job adaptation on the turnover intention of the nursing care provider and to contribute to the various problems of the nursing care provider in the long term. In order to confirm this as an empirical research task, risk factors and protection factors, general characteristics of the survey subjects, job adaptation and turnover intention were selected, and the risk factors and protective factors of caregivers' As a mediator. So Seoul. The results of the questionnaire survey were as follows: 291 caregivers in the elderly medical welfare facilities in Gyeonggi area. First, as the relationship between the risk factors and protective factors of occupational caregivers and occupational adaptation were more severe, the higher the maladjustment of the workplace culture, the more the job satisfaction and organizational commitment were adversely affected. The emotional support, The higher the information support, the more satisfied and satisfied the job. Second, the relationship between the risk factors of the caregiver and the protective factors and the turnover intention, the higher the conflict of caregivers, the more unstable the workplace, the more difficult it is to adapt to work culture, Respectively. Finally, as a result of verifying the mediating effect of occupational adaptation on the relationship between risk factors and protective factors and turnover intention of caregivers, job satisfaction, which is a sub-factor of job adaptation, It is shown that they play mediating roles only in the relationship between stress and turnover intention, and do not play a mediating role in the relationship between protective factor self - efficacy and social support and turnover intention. In other words, if caregivers feel satisfaction about their job, they can be less stressed on their jobs, improve their self-efficacy, and have a positive attitude toward social support. Also, it was found that the more the caregiver 's immersion into the organization, the less job stress and turnover intention decreased, but the self - efficacy and social support perception were not influenced. Based on this, the director of the facility should strive to stabilize the operation of the facility and provide high-quality services by seeking ways to improve conflict resolution and adaptation to the workplace culture so that nursing care workers can adapt to their work. And it is required to develop active management strategies and institutional support for improving job satisfaction and organizational commitment of caregivers.

• Korean Journal of Culture and Social Issue
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• v.21 no.3
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• pp.299-315
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• 2015

This study examines correlations among factors that reportedly affect adjustment to military life: self esteem, locus of control,, social support and stress coping strategies. The study also attempts to validate the structural model to enhance understanding of the process of adjusting to the military life. The subjects of the study were 447 listed soldiers from private to sergeant currently stationed and serving in an army post in Gangwon-Do, Korea. The following tools were used for this study. Based on self-inventory questionnaires, evaluations were made regarding self esteem, locus of control, social support, stress management, adaptation to military life. Reliability of each criterion was evaluated based on reliability examination, correlations among parameters were analyzed, and the structural equation model was validated to verify the research model. The results of the study can be summarized as follows. First, internal control and self esteem have significant positive influence on social support and stress coping. Second, internal control and self esteem affect adjusting to military life through social support and active coping, two factors mediated in this study to act as parameters between self-esteem and internal control and military adaption. This study emphasizes intervention such as establishment of a social support system and training for taking necessary actions as limitations of studies dealing with internal traits and military adaptation. Since the subjects of this study were chosen from an army based in a single location for convenience' sake, it may be difficult to generalize the results to analyze the entire military.

• Journal of Korean Academy of Nursing
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• v.27 no.2
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• pp.329-340
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• 1997

Resourcefulness was analyzed by Walker and Avant's method to make a theoretical framework for nursing. Also, the appropriate Korean terminology was identified.“Resourcefulness”means the ability to use internal and external resources to eliminate or to control stress. '자원동원성' is chosen to be the most appropriate term to reflect the concept of resourcefulness. Upon the concept analysis, availability, controllability, confidence, and self-instruction were identified as the defining characteristics of resourcefulness. Contrary to other work, "availability" means the ability to use the social resources as well as the internal cognitive-behavioral resources. “Controllability” means the ability to delay or control immediate gratification of one's needs or to divert one's own mood in order to solve problem. "Confidence" is the self-efficacy belief in one's control ability. "Self-instruction" is the cognitive ability to instruct oneself positively. Resourcefulness is learned by active experience, vicarious experience, and formal or informal instruction, and it is cued in the presence of a stressful situation or a need for change. As a result of the use of resourcefulness, the target behavior performed immediately and ultimately improve the quality of life or adaptation. Therefore, it is necessary to develop the instrument including the defining attributes identified in this study. Also, it is necessary to simultaneously analyze the related concepts of self-efficacy, self-control, and self-regulation for appropriate use.

• The Korean Journal of Rehabilitation Nursing
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• v.15 no.1
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• pp.1-10
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• 2012

Purpose: To identify Korean versions of self-reported sleep questionnaires for cross-cultural research and practice on sleep disturbance, we searched self-reported sleep questionnaires which have been developed to measure general sleep patterns. They should be qualitative, easy to take permission, do not obligate to pay, do not need any skill or professional help for scoring and have been reported high reliability and validity. Methods: General Sleep Disturbance Scale (GSDS), Leeds Sleep Evaluation Questionnaire (LSEQ), and Pittsburgh Sleep Quality Index (PSQI) were identified based on all criteria. Korean version of GSDS, LSEQ, and PSQI were developed according to the methods combined the guidelines for the process of cross-cultural adaptation of self-reported measures with decentering method. Results: Three Korean versions of self-reported sleep questionnaires were suitable for epidemiological study. They are not full length and easy to read. It requires less than 10 minutes for each subject to complete as well as for researchers to score. Conclusion: Psychometric analysis study is necessary to evaluate the reliability and validity of those Korean versions of self-reported sleep questionnaires.

• Journal of the Semiconductor & Display Technology
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• v.17 no.1
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• pp.6-11
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• 2018

With the development of the Internet of Things technology, a system that ensures the self-adaptability of an environment that includes various IoT devices is attracting public attention. The rules for determining behavior rules in existing self-adaptation systems are based on the assumption of changes in system members and environment. However, in the IoT environment, flexibility is required to determine the behavior rules of various types of IoT devices that change in real time. In this paper, we propose a rule configuration in a self-adaptive system using SWRL based on OWL ontology. The self-adaptive system using the OWL - SWRL rule configuration has two advantages. The first is based on OWL ontology, so we can define the characteristics and behavior of various types of IoT devices as an integrated concept. The second is to define the concept of a rule as a specific language type, and to add, modify and delete a rule at any time as needed. Through the rule configuration in the adaptive system, we have shown that the rule defined in SWRL can provide flexibility and deeper concept expression function to adaptability to IoT environment.

• Journal of Medicine and Life Science
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• v.17 no.3
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• pp.86-93
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• 2020

The study aimed to understand medical students' experiences of emotional changes, including their method of adapting to experiences, and the effect of the experiences in shaping their identities. We interviewed 12 medical students who were finishing their 1-year clinical internship in 2016. Data on their opinions and reasons for emotional changes during their school life were obtained. The descriptive phenomenological approach was applied to analyze the interviews. Their stress came from disappointment in themselves, competitive environment, observing a change in their personalities, meeting their parents' expectations, and interpersonal relations. The interviewees adjusted to the medical study by exercising self-control in their studies and daily lives, by practicing self-acceptance and observing their state of mind, and by breaking free from the competition-driven environment and obsession with grades. In addition, they cultivated endurance and found external support. Finally, they achieved self-efficacy and were comfortable in their identity as medical students. They still had to address the stress from working relationships and the difficulty in balancing studies and life. The medical students' self-evaluation and compulsive tendencies increased during the medical course due to the burden of studies. They evolved by learning self-control and introspection and seeking ways to adapt. Understanding this growth process of medical students will improve student support in medical schools.