• IMMUNE NETWORK
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• v.16 no.2
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• pp.134-139
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• 2016

Programmed death-1 (PD-1) is a strong negative regulator of T lymphocytes in tumor-microenvironment. By engaging PD-1 ligand (PD-L1) on tumor cells, PD-1 on T cell surface inhibits anti-tumor reactivity of tumor-infiltrating T cells. Systemic blockade of PD-1 function using blocking antibodies has shown significant therapeutic efficacy in clinical trials. However, approximately 10 to 15% of treated patients exhibited serious autoimmune responses due to the activation of self-reactive lymphocytes. To achieve selective activation of tumor-specific T cells, we generated T cells expressing a dominant-negative deletion mutant of PD-1 (PD-1 decoy) via retroviral transduction. PD-1 decoy increased IFN-γ secretion of antigen-specific T cells in response to tumor cells expressing the cognate antigen. Adoptive transfer of PD-1 decoy-expressing T cells into tumor-bearing mice potentiated T cell-mediated tumor regression. Thus, T cell-specific blockade of PD-1 could be a useful strategy for enhancing both efficacy and safety of anti-tumor T cell therapy.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.112-116
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• 2003

An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by Self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amino (-NH$_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au Substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The Surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 10$^2$CFU per mL.

• Journal of the Institute of Convergence Signal Processing
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• v.2 no.3
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• pp.79-85
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• 2001

We obtain a solution of inverse kinematic of 3 axis manipulator by using a self-organizing neral network(SONN) with a fuzzy compensator. The self-organizing neural network using the gaussian potential function as the activation function has one hidden layer in the first learning time. The network obtains the optimal number of node by increasing the number of hidden layer node through the learning, and the fuzzy compensator has the optimal loaming rate of neutral network. In this results, we can confirmed that the learning rate is improved and the rapid convergence to the steady-state.

• Computers and Concrete
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• v.19 no.6
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• pp.659-666
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• 2017

Based on the Arrhenius equations, several hydration exothermic models that precisely calculate the influence of concrete's self-temperature duration on its hydration exothermic rate have been presented. However, the models' convergence is difficult to achieve when applied to engineering projects, especially when the activation energy of the Arrhenius equation is precisely considered. Thus, the models' convergence performance should be improved. To solve this problem and apply the model to engineering projects, the relationship between fast iteration and proper expression forms of the adiabatic temperature rise, the coupling relationship between the pipe-cooling and hydration exothermic models, and the influence of concrete's self-temperature duration on its mechanical properties were studied. Based on these results, the rapid convergence of the hydration exothermic model and its coupling with pipe-cooling models were achieved. The calculation results for a particular engineering project show that the improved concrete hydration exothermic model and the corresponding mechanical model can be suitably applied to engineering projects.

• Biomolecules & Therapeutics
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• v.28 no.6
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• pp.519-526
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• 2020

Methamphetamine (MA) is one of the most commonly abused drugs in the world by illegal drug users. Addiction to MA is a serious public health problem and effective therapies do not exist to date. It has also been reported that behavior induced by psychostimulants such as MA is related to histone deacetylase (HDAC). MeBib is an HDAC6 inhibitor derived from a benzimidazole scaffold. Many benzimidazole-containing compounds exhibit a wide range of pharmacological activity. In this study, we investigated whether HDAC6 inhibitor MeBib modulates the behavioral response in MA self-administered rats. Our results demonstrated that the number of active lever presses in MA self-administered rats was reduced by pretreatment with MeBib. In the hippocampus of rats, we also found MA administration promotes GluN2B, an NMDA receptor subunit, expression, which results in sequential activation of ERK/CREB/BDNF pathway, however, MeBib abrogated it. Collectively, we suggest that MeBib prevents the MA seeking response induced by MA administration and therefore, represents a potent candidate as an MA addiction inhibitor.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1050-1059
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• 2019

The self diffusion, hole volume, and flow thermodynamic parameters of polyamide fibers were calculated from rheological parameters and crystallite size in order to study of flow segments in amorphous region. The stress relaxation of polyamide filament fibers were carried out in air and various solvents at various temperatures using the tensile tester with the solvent chamber. The rheological parameters were obtained by applying the experimental stress relaxation curves to the theoretical equation of the Ree-Eyring and Maxwell non-Newtonian model. It was observed that the rheological parameters of these polyamide filament fibers are directly related to the relaxation spectra, self diffusion, viscosities, and activation energies of flow segments.

• Journal of Information Technology Services
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• v.18 no.3
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• pp.95-115
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• 2019

In recent years, social media that has emerged with the development of network technology has changed the channels of information dissemination. The social Q&A community is a platform for knowledge-sharing activities in a question-and-answer manner based on Web 2.0. In knowledge-sharing activities, valuable new knowledge continues to be produced and will surely bring great benefits to individuals or businesses. In the social Q&A community, the user's subjective factors play a crucial role in influencing the user's continued use and participation in knowledge-sharing activities. In order for users to actively participate in knowledge-sharing activities in the community, it needs to grasp their subjective ideas. This study explores the issue of sharing knowledge by users of the social Q&A community "Zhihu", or how to drive community revitalization efforts from these. The three factors self-efficacy, self-development motivation, and social comparison tendencies were derived, and identify their relationship with knowledge-sharing activities and community-boosting efforts through empirical analysis. In addition, the influence of knowledge acquisition on knowledge provision was investigated through sense of reciprocity. Implications of the study findings and the future research directions were also discussed.

• Journal of KIISE:Software and Applications
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• v.35 no.9
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• pp.538-546
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• 2008

It has limits that system administrator deals with many problems occurred in systems because computing environments are increasingly complex. It is issued that systems have an ability to recognize system's situations and adapt them by itself in order to resolve these limits. But it requires much experiences and knowledge to build the Self-Adaptive System. The difficulty that builds the Self-Adaptive System has been problems. This paper proposes a technique that generates automatically the codes of the Self-Adaptive System in order to make the system to be built more easily. This Self-Adaptive System resolves partially the problems about ineffectiveness of the exceeded usage of the system resource that was previous research's problem and incorrect operation that is occurred by external factors such as virus. In this paper, we applied the proposed approach to the file transfer module that is in the video conferencing system in order to evaluate it. We compared the length of the codes, the number of Classes that are created by the developers, and development time. We have confirmed this approach to have the effectiveness.

• Journal of Korea Entertainment Industry Association
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• v.14 no.7
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• pp.79-94
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• 2020

The purpose of this study is to analyze the difference in the effect of presence for the activation of virtual reality sports on participation satisfaction, to suggest continuous screen golf exercise participation, and to provide empirical and academic data for the development of the entire virtual reality sports market. To achieve this purpose, the survey period was from March 13 to May 13, 2020, with five researchers and assistants. The purpose of this study and the questionnaire were fully explained to consumers who experienced screen golf directly, and 247 questionnaires were used as the final valid sample by making a questionnaire with self-administration method. The data processing method was the statistical program Windows SPSS 18.0. First, factor analysis and reliability analysis, second, frequency analysis mean(M) and standard deviation(SD), third, Scheffe analysis among t-test and One-way ANOVA analysis, fourth, correlation analysis between variables and multiple regression analysis were conducted. The results of this study through these methods and procedures are as follows. First, there was a significant difference in participation satisfaction of presence in gender, and participation period of general characteristics. Second, there was a high difference in social presence, social self-reliance, and Ego, which are sub-factors of Presence, in social satisfaction, psychological satisfaction, and physical satisfaction. Third, the sub-factors of Presence, Social Presence, Social Self-Reliance, and Ego, were found to have a high effect on the sub-factors of Participation Satisfaction, Social Satisfaction, and Psychological Satisfaction.

• Journal of Korean Academy of Nursing
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• v.26 no.4
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• pp.963-975
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• 1996

The purpose of this study was to call attention to the mental, physical and occupational hazards of the anticancer-drug-handling nurses by examining the possible urinary mutagenicity and measuring physical symptoms and stress level of the nurses exposed to anticancer drugs. The experimental group of the urinary mutagenicity assay was 14 nurses handling anticancer drugs at the medical wards of a hospital located in J city ; the control group was 12 psychiatric nurses of the same hospital. The test material was the nurses' 24hrs urine, which was concentrated by XAD-2 column chromatography. Tester strains were TA98(±S9 mix), TA100(±S9 mix), TA1535(±S9 mix) and TA1537(±S9 mix) ; Salmonella mammalian-microsomal test(Ames test) was employed for the urinary mutagenicity assay. The physical symptoms of which the nurses experienced were investigated through self-reports on open-questionnaires. The stress levels of the experimental group were measured by a stress measuring instrument developed by this author. Reliability of this instrument was found to be adequate (Cronbach's Alpha=0.9079). To ascertain the urinary mutagenicity of the experimental group, the mean and the standard deviation of the colonies of Tester strains appearing on the minimal plates were taken and compared differences between two groups. T-test was employed for the significance test of two groups. The physical symptoms were compared between the two groups through the analysis of the nurse' self-reports. The mean and standard deviation of the stress levels of the experimental group were also calculated and were examined through t-test. The results were summarized as follows : 1. The experimental group revealed significantly higher urinary mutagenicity both in the activation method test and the non-activation method test of the tester strains TA98, TA100 and TA1535. In the case of TA1537, two groups showed no difference in the non-activation method test, but the activation method revealed difference. 2. The physical symptoms were also much more frequently reported in the experimental group. 79.3% of the experimental group reported more than 1 kind of physical symptoms. On the other hand, 33.2% of the control group complained of 1 kind of physical symptom. The items with high symptom frequency were 'headache', 'itching sensation', 'corneal congestion', 'skin allergy' 3. The mean score of stress in the experimental group was 2.41(range 1-4). The experimental group showed the stress level above 2.0 in the 14 of 15 items in all. The highest stress level were recorded in the following items in the order quoted, 'I fear that anticancer drug may touch any part of body while handling it.', 'I feel concerned there is no protective countermeasure against anticancer drug handling.', 'I am afraid the anticancer drug handling may produce a fetal loss in the future'.